Proračun ukupnog sadržaja elektrona (TE ) na temelju dvofrekvencijskih mjerenja 10. Obrada RINEX formata podataka s ciljem generiranja položajnih zapisa 11. Osnovni elementi dinamike ionosfere 12. Struktura GPS/Glonass signala 13. Svemirsko vrijeme i njegov utjecaj na GNSS sustave 14. Precizno pozicioniranje s carrier phase mjerenjem 15. count/total YouTube TV - No long term contract.
Hrvatsko biološko društvo SOCIETAS BIOLOGORUM CROATICA Croatian Biological Society
12. HRVATSKI BIOLOŠKI KONGRES s međunarodnim sudjelovanjem th 12 CROATIAN BIOLOGICAL CONGRESS with International Participation Sveti Martin na Muri, 18. – 23. IX 2015.
ZBORNIK SAŽETAKA BOOK OF ABSTRACTS
Hrvatsko biološko društvo SOCIETAS BIOLOGORUM CROATICA Croatian Biological Society
12. HRVATSKI BIOLOŠKI KONGRES s međunarodnim sudjelovanjem 18. – 23. rujna 2015. Sveti Martin na Muri, Hrvatska th 12 CROATIAN BIOLOGICAL CONGRESS With International Participation 18th – 23rd September 2015 Sveti Martin na Muri, Croatia ZBORNIK SAŽETAKA BOOK OF ABSTRACTS Zagreb, 2015.
ZBORNIK SAŽETAKA 12. HRVATSKOG BIOLOŠKOG KONGRESA BOOK OF ABSTRACTS OF THE 12th CROATIAN BIOLOGICAL CONGRESS Urednici / Editors Göran Klobučar Nevenka Kopjar Marija Gligora Udovič Žaklin Lukša Dušan Jelić Odgovorni tehnički urednici / Technical Editors in Chief Göran Klobučar Nevenka Kopjar Hrvatsko biološko društvo Croatian Biological Society Zagreb, 2015. ISSN 1848‐5553 Ključni naslov: Zbornik sažetaka (Hrvatski biološki kongres s međunarodnim sudjelovanjem) Skraćeni ključni naslov: Zb. sažet. (Hrvat. biol. kongr. međunar. sudjel.) ii
Organizator kongresa i izdavač zbornika / Organiser of the Congress and Publisher of the Book of Abstracts Hrvatsko biološko društvo Croatian Biological Society Rooseveltov trg 6, HR‐10000 Zagreb, Hrvatska Tel: +385 (0)1 4877733; Fax: +385 (0)1 4826260 e‐mail: [email protected]‐sbc.hr URL: www.hbd‐sbc.hr Organizacijski i programski odbor / Organising and Program Committee: Göran Klobučar (Predsjednik / President), Višnja Besendorfer (Dopredsjednica / Vicepresident), Marija Gligora Udovič (Tajnica / Secretary), Anamaria Štambuk (Blagajnica / Treasurer), Biljana Balen, Nataša Bauer, Sunčica Bosak, Domagoj Đikić, Mirna Ćurković Perica, Damjan Franjević, Bojan Hamer, Nenad Jasprica, Petra Korać, Petar Kružić, Mladen Kučinić, Bojan Lazar, Žaklin Lukša, Nenad Malenica, Marko Miliša, Božena Mitić, Anđelka Plenković‐Moraj, Ana Previšić, Sandra Radić Brkanac, Dijana Škorić Znanstveni odbor / Scientific Committee: Nevenka Kopjar (Predsjednica / President), Dunja Leljak‐Levanić (Dopredsjednica / Vice President), Andreja Ambriović‐Ristov, Renato Batel, Vesna Benković, Branimir K. Hackenberger, Krunoslav Brčić‐Kostić, Vera Cesar, Marko Ćaleta, Helena Ćetković, Zdravko Dolenec, Jakov Dulčić, Jerka Dumić, Hrvoje Fulgosi, Sanja Gottstein, Bojan Hamer, Dubravka Hranilović, Jasna Hrenović, Stipan Jonjić, Mladen Kerovec, Marcelo Kovačić, Valter Kožul, Stjepan Krčmar, Marijana Krsnik‐Rasol, Gordana Lacković‐Venturin, Gordan Lauc, Bojan Lazar, Zlatko Liber, Žaklin Lukša, Zrinka Ljubešić, Renata Matoničkin Kepčija, Melita Mihaljević, Milorad Mrakovčić, Toni Nikolić, Nadica Oršolić, Mirjana Pavlica, Miroslav Plohl, Martina Podnar Lešić, Jasna Puizina, Ines Radanović, Mary Sopta, Zdenko Tkalčec, Zoran Tadić, Nikola Tvrtković, Đurđica Ugarković, Željka Vidaković‐Cifrek, Damir Viličić, Kristian Vlahoviček, Nedo Vrgoč, Davor Zahradka, Irina Zupan
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Tehnička potpora / Technical support: Ivana Bošnjak, Romana Gračan, Renata Horvat, Marija Ivković, Mišel Jelić, Maja Mejdandžić, Dorotea Polović, Maja Šrut, Vedran Vuković, Petar Žutinić Tehnički organizator kongresa / Technical support for registration, accommodation and excursions: PBZ Card d.o.o., putnička agencija / Travel agency; Radnička cesta 44, 10000 Zagreb, Croatia Pokrovitelji / Patrons: Ministarstvo znanosti, obrazovanja i sporta Republike Hrvatske / The Ministry of Science, Education and Sport of Republic of Croatia Hrvatska Akademija znanosti i umjetnosti / Croatian Academy of Sciences and Arts Agencija za odgoj i obrazovanje / Education and Teacher Training Agency Prirodoslovno‐matematički fakultet Sveučilišta u Zagrebu / Faculty of Science, University of Zagreb Biološki odsjek Prirodoslovno‐matematičkog fakulteta Sveučilišta u Zagrebu / Department of Biology, Faculty of Science, University of Zagreb Međimurska županija / Međimurje county Sponzori / Sponsors: ALFA d.d. ‐ Zagreb Bioinstitut d.o.o. ‐ Čakovec Biosistemi d.o.o. ‐ Zagreb Biovit d.o.o. ‐ Zagreb Gorea Plus d.o.o. ‐ Sveta Nedjelja Hrvatske vode INEL ‐ medicinska tehnika d.o.o. ‐ Zagreb Kefo d.o.o. ‐ Zagreb Merck d.o.o. ‐ Zagreb Nastavni zavod za javno zdravstvo dr. Andrija Štampar ‐ Zagreb RU‐VE d.o.o. ‐ Zagreb Školska knjiga d.d. ‐ Zagreb Založba Hart ‐ Ljubljana Slovenija iv
Cijenjene kolegice i kolege, dragi biolozi! Za one rijetke koji iz nekog samo njima poznatog razloga čitaju predgovore u knjigama sažetaka želim napomenuti da su vremena sve teža za organiziranje kongresa a poglavito kongresa koji su ovako sveobuhvatni kao što je to Hrvatski biološki kongres. U ovom 12‐om po redu Kongresu smanjili smo broj tema kako bi objedinili sudionike kongresa sa sličnim znanstvenim interesima te smo povećali broj simpozija koji se održavaju u sklopu kongresa. Također, odlučili smo se za Sveti Martin na Muri sa željom da istaknemo prirodne vrijednosti kontinentalne Hrvatske a posebice Međimurja. Pretpostavljam da je to imalo određeni utjecaj na vašu odluku o sudjelovanju na 12. Hrvatskom biološkom kongresu jer ovaj Zbornik sažetaka sadrži 8 plenarnih i 5 pozvanih predavanja te 125 usmenih i 143 posterska priopćenja. Želim stoga zahvaliti svim sudionicima ovog Kongresa, a posebice onima koji čitaju ove predgovore, na odluci da odvoje vrijeme i određena financijska sredstva kako bi sudjelovali na ovom već tradicionalnom kongresu koji, siguran sam, ima posebno mjesto u našim srcima. U ime Organizacijskog i programskog odbora Göran Klobučar Esteemed colleagues, dear biologists! For those of you who, for some unknown reasons, read forewords in Books of Abstracts I wish to emphasize that times are not favourable for organising congresses and most of all those that are so comprehensive as Croatian Biological Congress. In this twelfth CBC we reduced the number of congress topics in order to bring together biologists with similar scientific interests and we increased the number of satellite Symposia. Also, we decided to hold this Congress at Sveti Martin na Muri in order to accentuate values of continental Croatia and especially Međimurje region for us biologists. I suppose that these changes th might have had some influence on your decision to attend 12 Croatian Biological Congress as this Book of Abstracts contain 8 plenary and 5 invited lectures, 125 oral and 143 poster presentation. Therefore, I wish to thank all the participants of this Congress, especially those who took time to read this foreword, for the decision to invest their time and certain funds to participate in this already traditional Congress which has, I am sure, a special place in our hearts. On behalf of the Organising and Programme Committee Göran Klobučar
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Poštovane kolegice i kolege, sudionici 12. Hrvatskog biološkog kongresa! Biologija 21. stoljeća suočava se sa brojnim izazovima ‐ razvoj i primjena novih tehnologija mijenjaju mnoga saznanja o živom svijetu na svim razinama organizacije, a otvaraju se i nova područja istraživanja. Premda oduvijek vrlo dinamična znanost, suvremena biologija danas možda brže i više nego ikada dolazi do vrijednih spoznaja, koje preispituju i pokreću nova istraživanja čak i u okvirima 'klasičnih' bioloških disciplina. Biolozi su i dalje vrlo važan i nezaobilazan dio složenih istraživačkih timova i u različitim srodnim znanostima. Povećana količina novih znanja i činjenica stavlja izazove i pred biologe koji se bave obrazovanjem, počevši od osnovnih škola pa do visokoškolskih ustanova, koji moraju naći nova rješenja i putove kojima će ta nova znanja što bolje prenijeti na današnje generacije učenika i studenata. Jedna od najvažnijih pretpostavki na kojima se zasniva suvremena znanost jest potreba za što bržim i dinamičnijim protokom informacija. Znanstveni skupovi stoga postaju vrlo važna mjesta susreta stručnjaka i istraživača koji aktivno komuniciraju rezultate svojih istraživanja i imaju mogućnost međusobnog upoznavanja te boljeg povezivanja radi što smislenije i svrsishodnije organizacije nekih budućih istraživačkih projekata i suradnja. Hrvatsko biološko društvo kroz organizaciju svojeg dvanaestog kongresa uspješno nastavlja dugogodišnju tradiciju okupljanja domaćih, ali i inozemnih znanstvenika i stručnjaka koji aktivno djeluju u području biologije i srodnih znanstvenih grana. Nadamo se da će, svojim bogatim znanstvenim programom, ovogodišnji kongres ispuniti očekivanja sudionika jer su zastupljena sva područja od interesa naših biologa, a kroz specijalizirane simpozije moći će se dobiti dobar uvid u najnovija istraživanja i suvremena kretanja u pojedinim biološkim disciplinama. Izborom plenarnih predavanja također smo nastojali istaknuti neka od trenutačno najvažnijih i vrlo aktivnih područja u okviru biologije, čiji vrijedni rezultati dovode do novih i ključnih spoznaja u funkcioniranju živog svijeta i nude smjerove prema nalaženjima odgovora na mnoge, još uvijek neistražene, biološke fenomene. Zadovoljni smo što je 12. Hrvatski biološki kongres okupio veliki broj biologa svih dobnih skupina i što su se studenti biologije kroz prijavu rezultata svojih istraživanja već sada aktivno uključili u istraživački prostor jer je to najbolji put za daljnji napredak biologije u okvirima hrvatske, ali i svjetske znanosti te dobar početak za njihov osobni profesionalni razvoj. Također moramo istaknuti da ovogodišnji kongres ima i naglašen međunarodni karakter kroz veliki broj prijavljenih sudionika iz inozemstva, što upućuje na činjenicu da će naši skupovi ubuduće vjerojatno prerasti nacionalne okvire. To će zasigurno utjecati i na organizaciju kongresa biologa u budućnosti, dajući im novu kvalitetu kako u znanstvenom, tako i stručnom smislu. Ova knjiga sadrži sve u roku pristigle sažetke usmenih i posterskih priopćenja prijavljenih za 12. Hrvatski biološki kongres. Znanstveni odbor proveo je recenzijski postupak i sažetke tehnički uredio i ujednačio prema zadanom predlošku. Nadamo se da će čitatelji u ovoj knjizi sažetaka naći korisne informacije i poticaj za svoj znanstveni i stručni rad, a svim sudionicima kongresa želimo uspješan rad i što bolju prezentaciju rezultata svojih istraživanja na 12. Hrvatskom biološkom kongresu. U ime Znanstvenog odbora 12. Hrvatskog biološkog kongresa Nevenka Kopjar, Predsjednica Znanstvenog odbora vi
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Esteemed colleagues, dear participants of the 12 Croatian Biological Congress! st The field of biology in the 21 century is faced with many challenges – the development and application of new technologies has greatly affected our knowledge regarding the living world at all levels of organization and uncovered many new and interesting research areas. Although always a very dynamic field of study, modern biology is probably more efficient and faster in acquiring valuable information now than ever. This leads to critical re‐examinations and initiations of new research, even within more 'classical' biological disciplines. Biologists are still a very important and vital part of complex research teams in different related scientific fields. The increased amount of new knowledge also brings challenges for biologists involved in education, starting from primary school to university level. They now have to find new solutions and ways to present this new knowledge to their students. One of the most important assumptions that underlie modern science is the need for a faster and more dynamic flow of information. Conferences have therefore become an important place for meeting researchers and experts who communicate the results of their research and engage in fruitful discussions, leading to joint research projects. th By organizing its 12 congress, the Croatian Biological Society successfully continues the longstanding tradition of joining scientists and experts active in the field of biology and related scientific fields. We hope that the enticing scientific program of this year's congress will meet the expectations of the participants because it was designed to cover almost all areas of interest. Through specialized symposia, the participants will be able to get good insight into the state‐of‐the‐art research trends in different biological disciplines. Through our selection of plenary lectures, we tried to stress some of the most important and highly active areas within biology today. The valuable results they have provided new directions towards finding answers to the many still unexplored biological phenomena. th We are pleased that the 12 Croatian Biological Congress brought together a large number of biologists of all ages, especially so many biology students. By presenting the results of their research, they will commence their own active involvement in the world of true research. This year's congress also boasts a more international character with many international participants, which points to the fact that our future meetings will probably outgrow only Croatia as their venue. This will certainly affect the organization of the biological congress in the future, giving them a new quality both scientifically and professionally. th This Book of Abstracts contains all abstracts submitted for the 12 Croatian Biological Congress. The Scientific Committee tried, within the best of its capability, to edit the received abstracts and conducted a peer review process. We hope that our readers will find useful information in this Book and deem it helpful in their scientific and professional th work. We would also like to wish all of the participants of the 12 Croatian Biological Congress a successful participation and presentation of their findings. th On behalf of the Scientific Committee of the 12 Croatian Biological Congress Nevenka Kopjar, President of the Scientific Committee
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SADRŽAJ CONTENTS
v Predgovor / Foreword PLENARNA PREDAVANJA / PLENARY LECTURES Uvodno plenarno predavanje / Introductory plenary lecture: PL‐1 2 I. Štagljar Protein interaction networks in health & disease PL‐2 2 R. Bakarić, T. Domazet‐Lošo The rise and fall of genome complexity and other macroevolutionary stories PL‐3 3 P. Nosil Divergence and constraint in the origin of new species PL‐4 3 A. Šorgo Socio‐scientific issues: a challenge for biology teachers PL‐5 4 D. Bar‐Zvi Plant response to abiotic stress PL‐6 4 A. Herrel Analyses of morphology, physiology, and microbial diversity after a recent dietary switch in a lizard PL‐7 5 D. Drobne How much room is left for conventional biology in modern nanoscale and omics research Završno plenarno predavanje / Closing plenary lecture: PL‐8 5 G. Lauc Glycans as integrators of genes and environment – an often‐ignored layer of biological complexity USMENA IZLAGANJA / ORAL PRESENTATIONS Konzervacijska biologija, zaštita prirode i okoliša Conservational biology, nature and environment protection O‐1 8 I. Sučić, L. Katušić Vrijednost faunističkih istraživanja kao temelja za planiranje aktivne zaštite prirode ‐ projekt integracije u EU Natura 2000 (NIP) Faunistic research values as the baseline for planning active nature conservation ‐ EU Natura 2000 Integration Project (NIP project) th
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9 O‐2 A. Štefan, S. Stolton, D. Porej, K.Z. Ivanić Assessing protected area values and benefits in the Dinaric arc O‐3 9 E. Patčev, M. Grgurev, A. Avdaković, M. Maslać, A. Bukovac, H. Peternel Prva primjena radarske ornitologije na području balkanske regije The first application of the radar ornithology in the Balkan region O‐4 10 D. Hamidović, S. Desnica Međunarodno važna podzemna skloništa za šišmiše Hrvatskoj ‐ primjena u očuvanju i zaštiti Internationally important underground sites for bats ‐ conservation and protection application O‐5 11 D. K. Hackenberger, T. Vondrak Sustav za multispektralnu analizu vegetacije za biološka i ekološka istraživanja Multispectral vegetation analysis system for biological and ecological research O‐6 12 M. Stanković Endemic and relicts species in special nature reserve Zasavica O‐7 13 M. Maslać, A. Partl, Z. Mesić, O. Antonić Lišajevi kao bioindikatori kvalitete zraka na području motovunske šume Lichens as bioindicators of air quality in the Motovun forest area O‐8 14 A. Vucić, D. Peroš‐ Pucar, B. Mitić, I. Hrga, B. Pucar Utjecaj meteoroloških parametara na varijacije dvosatnih koncentracija peludnih zrnaca čempresa (Cupressaceae), grad Zadar, Hrvatska Meteorological influence on diurnal Cupressaceae pollen variation (Zadar, Croatia) O‐9 15 J. Freyhof, S. Weiss, A. Adrović, M. Ćaleta, A. Duplić, B. Hrašovec, B. Kalamujić, Z. Marčić, D. Milošević, M. Mrakovčić, D. Mrdak, M. Piria, P. Simonović, S. Šljuka, T. Tomljanović, D. Zabric Mladica (Hucho hucho) na Balkanu: rasprostranjenost i budući utjecaji hidroenergetskih planova The huchen (Hucho hucho) in the Balkan region: distribution and future impacts by hydropower development O‐10 16 D. Pithart, A. Duplić, P. Kutleša, I. Petrov Rančić, N. Trenc Procjena usluga ekosustava na području Drava‐Molve prema tri potencijalna scenarija Assessment of ecosystem services for the area Drava‐Molve according three potential scenarios O‐11 18 B. K. Hackenberger, O. Antonić, Ž. Lončarić Modeliranje populacije vrste slabo istraženih karakteristika – primjer popovske gaovice (Delminichthys ghetaldii) na području Omble Modeling population of a poorly investigated species ‐ example on Delminichthys ghetaldii in the river Ombla th
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19 O‐12 K. Žganec, F. Antonelli, I. Popović Dujmović, N. Jeran U potrazi za očuvanim rijekama: identifikacija najznačajnijih rijeka za zaštitu prirode u Hrvatskoj Looking for pristine rivers: identification of the most outstanding rivers of high conservation value in Croatia O‐13 20 A. Duplić, D. Jelić, B. Barić, B. Janev Hutinec, P. Kutleša, I. Cizelj Protokol procjene rizika od invazivnosti stranih vrsta kornjača u Hrvatskoj Protocol for the invasiveness risk of the alien turtle species in Croatia O‐14 21 D. Matošević, I. Franić, I. Lukić Kako se boriti protiv invazivne vrste? Biološko suzbijanje kestenove ose šiškarice unesenom vrstom parazitoida How to control invasive species? Classical biological control of Dryocosmus kuriphilus with introduced parasitoid species O‐15 22 B. Glamuzina Štete i koristi invazija unešenih riba u slivu Neretve Damages and benefits of introduced fish invasions in the Neretva river watershed O‐16 23 K. Jelić, J. Jeremić Protokol za dojavu i djelovanje u slučaju pronalaska uginulih, bolesnih ili ozlijeđenih strogo zaštićenih morskih životinja Protocol for alerting and monitoring of dead, sick or injured strictly protected marine species O‐17 24 D. Hamidović, V. Lindić, P. Krstinić Kada i da li ljudi i šišmiši mogu dijeliti isto sklonište? When and if humans and bats may share the same roost 2. Simpozij edukacije biologije 2nd Biology Education Symposium O‐18 25 B. Bajd Simplified identification keys for organisms O‐19 26 D. Garašić, I. Radanović, Ž. Lukša, M. Sertić Perić Afinitet učenika za izbor zanimanja u području biologije Students' affinities for professional carrier in the field of biology O‐20 27 Ž. Lukša, T. Šalamon, T. Dragić Runjak, N. Sinković Utjecaj aktivnosti upoznavanja biljaka na motivaciju i znanje učenika The impact of activities of familiarizing students with plants on the motivation and knowledge of students th
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28 O‐21 D. Novoselić, N. Kletečki, M. Bulić Motivacija učenika sedmih razreda za stjecanje znanja iz nastavnog predmeta biologija Seventh graders’ motivation for knowledge acquisition of elementary school biology O‐22 29 A. Mojsović Ćuić, O. Digula E‐biologija na Zdravstvenom veleučilištu E‐biology at the University of applied health sciences O‐23 30 A. Belančić Uporaba digitalnih nastavnih sadržaja u nastavi biologije The use of digital educational content in teaching biology O‐24 31 L. Šarić, M. Varga Projektna terenska nastava na obroncima Medvednice Project field classes on the slopes of Medvednica O‐25 32 M. Cindrić, D. Crnčec, M. Mesarić Analiza stavova i interesa učenika srednjih škola o prirodnim vrijednostima Međimurske županije Middle school students´ attitudes and interes analysis about their natural values in the Međimurje county O‐26 33 M. Labaš Horvat, M. Rašan Razvoj jezičnih, društvenih i upravljačkih kompetencija u učeničkim istraživačkim projektima u programu GLOBE i biologiji Development of linguistic, social and management competencies in GLOBE and biology student research projects O‐27 34 Z. Pongrac Štimac, R. Halapir Franković Predstavljanje rezultata potprojekata istraživanje i rekreacija u prirodi i nastavne metode u biologiji i kemiji u okviru projekta afirmativna nastava i inovativno učenje i poučavanje u gimnazijama u okviru hrvatskog kvalifikacijskog okvira ‐ IPAQ Peta Presenting the results of subprojects research and recreation in nature and teaching methods in biology and chemistry in the framework of affirmative and innovative learning and teaching in gymnasiums within the croatian qualifications framework ‐ IPAQ Peta O‐28 36 Ž. Kadi, J. Pavlic Primjer integriranja različitih nastavnih predmeta na temu zaštite lokaliteta Bedekovićeve grabe An example of integrating different school subjects focusing on protecting the site „Bedekovićeve grabe“ O‐29 37 N. Graovac, E. Merdić Istraživanje stavova učenika o prehrani Pupil's diet‐related attitudes to healthy lifestyle th
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38 O‐30 A. Špernjak Anatomy and physiology of human body teaching with natural materials O‐31 38 M. Ruščić, I. Podrug, D. Sirovina Istraživanje učeničkog razumijevanja osnovnog biološkog koncepta menstruacijskog ciklusa Resarch of student understanding of basic biological concept of the menstrual cycle O‐32 39 D. Crnčec Analiza zastupljenosti različitih tipova zadataka i njihovih kognitivnih razina u testovima županijskih natjecanja biologije za osme razrede od 2009.‐2014. godine Analysis of the representation of different types of tasks and their cognitive levels in county‐level competition tests in biology for eighth grades from 2009 to 2014 40 O‐33 S. Rašan, M. Rašan Procjena kvalitete pitanja iz prirode i društva za 4. razred OŠ Estimating the quality of test questions from nature and society in the fourth grade O‐34 42 V. Begić, M. Bastić, I. Radanović Utjecaj kvalitete znanja učenika na rješavanje zadataka viših kognitivnih razina Influence of students’ knowledge on performance in solving complex cognitive tasks O‐35 43 L. Lugar, A. Mustać Uspješnost učenika 8. razreda u rješavanju pisanih zadataka iz biologije Efficiency of the 8th grade pupils in solving written assignments in biology Evolucija, sistematika, filogenija i biogeografija Evolution, systematics, phylogeny and biogeography O‐36 44 I. Buj, Z. Marčić, M. Ćaleta, T. Mihinjač, R. Šanda, D. Zanella, P. Mustafić, M. Mrakovčić Evolucija u Dinaridima – analiza filogeografske i populacijske genetske strukture riba roda Delminichthys Evolution in Dinarids – integrative analysis of phylogeography and population genetic structure of the fish genus Delminichthys O‐37 45 M. Jelić, G. Klobučar, F. Grandjean, J. Amouret, D. Franjević, M. Futo, D. Ghia, G. Fea, M. Govedič, M. Vucić, N. Puillandre, I. Maguire Filogenija i filogeografija bjelonogog raka (Austropotamobius pallipes kompleks) u rijekama jadranskog bazena Phylogeny and phylogeography of the white‐clawed crayfish (Austropotamobius pallipes complex) in the Adriatic basin rivers O‐38 46 F. Rebrina, J. Skejo, N. Tvrtković Prvi rezultati inventarizacije vrsta žohara, bogomoljki i ravnokrilaca (Insecta: Polyneoptera) šireg th
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područja planine Dinare First results of inventarisation of Blattodea, Mantodea and Orthoptera (Insecta: Polyneoptera) of the Dinara mountain area 47 O‐39 L. Lovrenčić, J. Lajtner, M. Podnar Molekularno‐filogenetička i filogeografska analiza populacija vrste Holandriana holandrii (C. Pfeiffer, 1828) (Mollusca: Gastropoda) u Hrvatskoj Molecular phylogenetic and phylogeographic analysis of populations of Holandriana holandrii (C. Pfeiffer, 1828) (Mollusca: Gastropoda) in Croatia O‐40 48 M. Šrut, A. Štambuk, A. Erdelez, D. Polović, M. Furdek, M. Peharda, G. Klobučar Utjecaj obalnog onečišćenja na epigenetiku i reproduktivni fitnes volka Hexaplex trunculus Impact of coastal pollution on epigenetics and reproductive fitness of marine snail Hexaplex trunculus O‐41 49 A. Štambuk, S. Dennis, D. Polović, M. Šrut, V. Soria‐Carrasco, Z. Gompert, V. Baković, G. Klobučar, P. Nosil Brza evolucija dagnje pod utjecajem onečišćenja ‐ eksperimentalna genomika Experimental genomic tests for pollution driven rapid evolution in the Mediterranean mussel O‐42 50 D. Polović, S. Dennis, A. Štambuk, M. Šrut, V. Soria‐Carrasco, Z. Gompert, V. Baković, G. Klobučar, P. Nosil Genetička arhitektura morfoloških obilježja dagnje Mytilus galloprovincialis Genetic architecture of morphological traits in the mediterranean mussel Mytilus galloprovincialis O‐43 51 N. Jantol, T. Kekić Analiza raznolikosti kloroplastne DNA i filogeografija ljekovite kadulje (Salvia officinalis L.) Analysis of chloroplast DNA diversity and phylogeography of common sage (Salvia officinalis L.) O‐44 52 I. Rešetnik, D. Baričevič, D. Batîr Rusu, K. Carović‐Stanko, P. Chatzopoulou, Z. Dajić‐Stevanović, M. Gonceariuc, M. Grdiša, D. Greguraš, A. Ibraliu, M. Jug‐Dujaković, E. Krasniqi, Z. Liber, S. Murtić, D. Pećanac, I. Radosavljević, Gj. Stefkov, D. Stešević, I. Šoštarić, K. Varbanova, Z. Šatović Genetska raznolikost prirodnih i kultiviranih populacija dalmatinske kadulje (Salvia officinalis L., Lamiaceae) Patterns of genetic diversity in wild and cultivated populations of dalmatian sage (Salvia officinalis L., Lamiaceae) O‐45 54 I. Radosavljević, Z. Šatović, Z. Liber Klonalna struktura endemične i ugrožene kratkozupčaste kadulje Salvia brachyodon (Lamiaceae) utvrđena mikrosatelitnim biljezima: značenje za upravljanje i zaštitu Microsatellite evidence for high clonality in narrow endemic and endangered Salvia brachyodon (Lamiaceae): implications for managment and conservation O‐46 55 J. Mlinarec, D. Franjević, L. Bočkor, V. Besendorfer th
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Evolucija, raznolikost i filogenija 5S rDNA jedinica ponavljanja u diploidnim i poliploidnim vrstama iz tribusa Anemoninae (Ranunculaceae) Evolution, diversity and phylogeny of 5S rDNA unit arrays in diploid and polyploid members of the tribe Anemoninae (Ranunculaceae) 56 O‐47 N. Koletić, M. Novosel, N. Kević, D. Franjević Evolucija mahovnjaka rekolonizacijom slatkovodnih ekosustava Evolution of bryozoans via recolonisation of freshwater ecosystems O‐48 57 M. Djurakic, A. Herrel Room to maneuver: physical environment constraints the pattern and rate of carapace shape evolution in Chelonians O‐49 58 M. Djurakic, V. Milankov A recognition of species taxon is a tail of microevolutionary processes in Testudo marginata: an evidence of a sex‐specific environmentally‐induced variation of plastron morphology 1. Balkanski herpetološki simpozij u suorganizaciji s Hrvatskim herpetološkim društvom 1st Balkan Herpetological Symposium in co‐organization with Croatian Herpetological Society IO‐1 59 M. Podnar, I. Grbac, N. Tvrtković, B. Bruvo Mađarić, W. Mayer Komparativna filogeografija tri vrste zapadnobalkanskih gušterica (Reptilia, Lacertidae) preklapajućih areala Comparative phylogeography of the three widely codistributed endemic Western Balkans lacertid lizards (Reptilia, Lacertidae) IO‐2 60 M. Lukač Zarazne bolesti vodozemaca – što se već zna, što je novo, a što treba imati na umu kako bi se spriječilo njihovo izumiranje Emerging amphibian diseases – what is known, what is new and what should be borne in mind to prevent their extinction O‐50 61 E. Padoa‐Schioppa, M. Polo Distribution, abundance and detection probability of Lacertidae in four Croatian islands O‐51 62 S. Baškiera, D. Jelić Učestalost živorodne gušterice, Zootoca vivipara Lichtenstein 1823 na području zapadnog Balkana How common is the common lizard, Zootoca vivipara Lichtenstein 1823 in West Balkan area? O‐52 63 A. Zimić Distribution (horizontal and vertical) and conservation status of herpetofauna of Sarajevo Canton and its wider area (Bosnia and Herzegovina) th
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63 O‐53 E. Kletečki, S. Leiner, M. Podnar Rezultati prve godine sustavnih istraživanja sastava, distribucije i brojnosti herpetofaune NP 'Plitvička jezera' Composition, distribution, and abundance of amphibians and reptiles of the Plitvice Lakes National Park during the first year of systematic research O‐54 64 K. van Lieshout, S. van Straten, M.J.A. Weterings, I.M. Dümmer, D. Jelić Ecological limitations and past events affect species distribution: assessing the quality of spatial distribution models for two reptile species O‐55 65 K. van Lieshout, S. van Straten, M.J.A. Weterings, I.M. Dümmer, D. Jelić Preliminary data on the assessment of the effectiveness of protected areas for two reptile species using spatial distribution modeling O‐56 65 M. Vujanović, D. Jelić Procjena osjetljivosti faune vodozemaca i gmazova u Hrvatskoj i Bosni i Hercegovini uz definiranje važnih herpetoloških područja Sensitivity assessment of fauna of amphibians and reptiles of Croatia and Bosnia and Herzegovina with detection of important herpetological areas O‐57 66 K. Ljubisavljević, L. Polović, V. Iković, S. Vuksanović, T. D. Vukov Habitat requirements and abundance of Dinarolacerta mosorensis and D. montenegrina in the mountains of Montenegro O‐58 67 M. Zadravec, R. Cesarec, Z. Tadić Biologija poskoka u kamenolomu Bizek Biology of the nose‐horned viper in the Bizek quarry O‐59 68 A. Ćurić, A. Zimić, D. Jelić New data and distribution of common spadefoot toad Pelobates fuscus (Laurenti, 1768) in Western Balkans O‐60 69 K. Čavlović, D. Karaica, I. Buj, V. Mičetić Stanković, L. Choleva, D. Jelić Komparativna morfologija, ekologija i analiza strukture hrvatske populacije Pelophylax esculentus kompleksa Comparative morphology, ecology and structure analysis of the Pelophylax esculentus complex population in Croatia O‐61 71 M. Jelić, M. Vucić, G. Klobučar, P. Korlević, D. Đikić, D. Franjević, D. Jelić Molekularna istraživanja zelenih žaba (rod Pelophylax) u Hrvatskoj – preliminarni rezultati Molecular study on water frogs (genus Pelophylax) in Croatia – preliminary results O‐62 72 T. Vološen, B. Prakljačić, D. Karaica, D. Jelić
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Procjena starosti jedinki zelenih žaba roda Pelophylax (Fitzinger, 1843) iz populacije sa Skadarskog jezera, Crna Gora Age estimation of green frog individuals belonging to the Pelophylax genus (Fitzinger, 1843) from the Lake Skadar population, Montenegro 72 O‐63 M. Vucić, M. Jelić, B. Prakljačić, G. Klobučar, D. Jelić Određivanje vrsta i hibrida zelenih žaba (rod Pelophylax) sa Skadarskog jezera (Crna Gora) analizom molekularnih biljega Determination of species and hybrids of water frogs (genus Pelophylax) from the Skadar Lake (Montenegro) using molecular markers O‐64 74 Lj. Tomović, A. Urošević, T. D. Vukov, R. Ajtić, K. Ljubisavljević, I. Krizmanić, D. Jović, N. Labus, S. Đorđević, M. L. Kalezić, G. Džukić, L. Luiselli Threatening levels and extinction risks based on distributional, ecological and life‐history datasets (DELH) versus IUCN criteria – example of Serbian reptiles O‐65 74 V. Vergilov, N. Tzankov, B. Zlatkov Hemipenial morphology as a useful complementary tool supporting the full species status of closely related scincid lizards (Scincidae, Sauria, Squamata) O‐66 75 I. Cizelj, M. Lukač, D. Jelić, I. Peranić Ex‐situ držanje autohtonih vodozemaca i gmazova u Zoološkom vrtu grada Zagreba u svrhu edukacije, zaštite i istraživanja Ex‐situ keeping of native amphibians and reptiles in Zagreb Zoo for education, conservation and research purposes O‐67 76 A. Golubović “I was a Ninja at your age” said an elderly tortoise to a hatchling – ontogeny shift in agility O‐68 77 E. Šunje, D. Jelić, M. Müller, R. Škrijelj, V. Helfer Comparative morphology of Salamandra atra prenjensis (Bosnia and Herzegovina) with its nominotypic subspecies Salamandra atra atra (Austria) O‐69 77 A. Ivanović, J.W. Arntzen Application of micro CT scanning and 3D geometric morphometrics in exploring evolution of skull shape in Triturus newts (Caudata: Salamandridae) O‐70 78 E. Saçdanaku, I. Haxhiu Data about Striped‐necked Terrapin, Mauremys rivulata in Vlora Bay, Albania O‐71 78 C. Samoila, D. Cogalniceanu, I. Ruben, M. Bogdan, A.Dumitrescu Modeling the potential distribution of the Spur‐thighed tortoise (Testudo graeca) and the Hermann’s tortoise (T. hermanni) at the northern limit of their range in Romania th
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79 O‐72 D. Stanković, Š. Gorički, M. Năpăruș‐Aljančič, M. Pavićević, M. Kuntner, A. Snoj, G. Aljančič Detection of Proteus eDNA in karst groundwater O‐73 80 A. Štih, I. Burić Značajke populacije riječne kornjače, Mauremys rivulata iz Konavoskog polja Characteristics of the Balkan Terrapin population from Konavle field Biologija mora Marine Biology O‐74 81 J. Dulčić, B. Dragičević Trenutni status bioraznolikosti ihtiofaune Jadranskoga i Sredozemnoga mora The current status of the Adriatic and Mediterranean fish biodiversity O‐75 82 P. Lučić, J. Njire, P. Kružić, B. Pestorić, D. Drakulović Mikrozooplankton Bokokotorskog zaljeva i obalnog područja južnog Jadrana Microzooplankton in the Boka Kotorska Bay and in the coastal region of the south Adriatic Sea O‐76 83 S. Bosak, M. Mejdandžić, I. Bošnjak, J. Godrijan, Z. Ljubešić Taksonomija i prostorna raspodjela fitoplanktona u južnom Jadranu; zimski aspekt Taxonomy and spatial distribution of phytoplankton in the south Adriatic; winter aspect O‐77 84 A. Žuljević, B. Antolić Usklađeno razmnožavanje algi roda Ulva Synchronized reproduction of Ulva species O‐78 85 D. Lučić, I. Onofri, M. Miloslavić, B. Gangai, I. Ivančić Novonastale promjene faune hidromeduza sjevernog Jadrana Recent changes in the northern Adriatic hydromedusan fauna O‐79 86 M. Miloslavić, D. Lučić Vremenska dinamika zajednica kalanoidnih kopepoda u izoliranom morskom jezeru (Veliko jezero, južni Jadran): veze s klimatskim promjenama na većoj skali Temporal patterns of the calanoid copepod community in Veliko jezero, an isolated marine lake (south Adriatic Sea): links to a larger‐scale climate changes 87 O‐80 G. Pleslić, G. Kett, N. Rako Gospić, T. Vučur, D. Holcer, J. Miočić‐Stošić, P. Mackelworth Utjecaj strategije hranjenja na društvenu strukturu dobrih dupina (Tursiops truncatus) u istočnom Jadranu, Hrvatska Influence of foraging strategies on social structure of bottlenose dolphins (Tursiops truncatus) in eastern Adriatic Sea, Croatia th
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88 O‐81 A. Jaklin, D. Pavičić Hamer, A. Baričević, W.E.G. Muller, R. Batel, B. Hamer Prilog poznavanju ekologije spužve Suberites domuncula (Olivi, 1872): preko morfologije do analize populacije Contribution to the ecology of the sponge Suberites domuncula (Olivi, 1872): from morphology to population analyses O‐82 89 I. Cvitković, M. Despalatović, A. Žuljević, S. Rožić, B. Antolić Ekološki status livada morske cvjetnice Posidonia oceanica uzduž hrvatske obale Ecological status of Posidonia oceanica meadows along the Croatian coast O‐83 90 M. Bilan, G. Dionísio, R. Calado, R. Rosa Rani razvojni stadiji dva tropska puža (Opisthobranchia) u klimatskim promjenama Ontogenic development of two tropical Opisthobranch mollusks in a changing ocean O‐84 91 A. Jaklin, F. Marin, D. Medaković Morfologija i struktura kućice puževa stražnjoškržnjaka Aplysia spp. Morphology and structure of the opisthobranch gastropod shell Aplysia spp. O‐85 92 R. Gračan, T. Polak, G. Lacković, B. Lazar Životni ciklus psa mekuša (Mustelus punctulatus) u sjevernom i srednjem Jadranskom moru Life –history traits of a Blackspotted smooth‐hound (Mustelus punctulatus) in the northern and central Adriatic Sea O‐86 93 P. Kružić, M. Kvarantan, S. Šaškor, V. Lonić, S. Motah, M. Andrić Naselja koralja na koraligenu pučinskih pličina u istočnom dijelu Jadranskog mora The coral assemblages of an off‐shore coralligene banks in the eastern Adriatic Sea O‐87 94 I. Bošnjak, H. Mihanović, I. Cetinić, P. Kružić, S. Bosak, M. Mejdandžić, Z. Ljubešić Prostorna raspodjela biooptičkih i termohalinih svojstava u sjeverozapadnom dijelu južnojadranske kotline Spatial distribution of biooptical and thermohaline characteristics in NW part of southern Adratic pit O‐88 96 P. Kružić Koralji podmorskih špilja Jadranskog mora Coral fauna of submerged marine caves in the Adriatic Sea 6. Simpozij Hrvatskog Društva za biljnu biologiju th 6 Symposium of the Croatian Society of Plant Biologists O‐89 97 N. Bauer, M. Juranić, M. Močibob, G. Razdorov, D. Leljak‐Levanić Protein TaMAB2 sudjeluje u remodeliranju kromatina i metabolizmu RNA u zigoti i 2‐staničnom proembriju pšenice th
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Wheat MATH‐BTB protein TaMAB2 participates in chromatin remodelling and RNA metabolism in zygote and 2‐celled proembryo 98 O‐90 J. Antunović Dunić, Lj. Mihaljević, D. Šimić, A. Lalić, J. Kovačević, V. Cesar Oporavak klijanaca ječma rehidracijom nakon stresa izazvanog sušom Recovery of drought stressed barley seedlings by re‐watering O‐91 99 S. Mlinarić, V. Cesar, H. Lepeduš Fotosintetski odgovor mladih i razvijenih listova smokve na visoki intenzitet svjetlosti Photosynthetic response of young and mature fig leaves to high irradiation O‐92 100 L. Begović, D. Pavoković, S. Mlinarić, A. Lalić, V. Cesar Određivanje izoformi peroksidaza skupine III i lakaza tijekom razvoja stabljike ječma (Hordeum vulgare L.) Determination of class III peroxidase and laccase isoforms during development of barley (Hordeum vulgare L.) stem O‐93 101 I. Šola, D. Poljuha, J. Bilić, S. Dudaš, T. Bilušić, J. Markić, G. Rusak Usporedba gastrointestinalne stabilnosti hrvatskih divljih jestivih biljaka: analiza fenolnih spojeva Comparison of gastrointestinal stability between Croatian wild edible plants: case of phenolics O‐94 103 P. Peharec Štefanić, K. Perica, K. Landeka, D. Bar‐Zvi, B. Balen ASR slični proteini otkriveni u in vitro kulturi kaktusa Mammillaria gracilis ASR‐like proteins detected in in vitro‐grown cactus Mammillaria gracilis 3. Simpozij Hrvatskog entomološkog društva 3rd Symposium of the Croatian Entomology Society O‐95 104 T. Bogdanović, M. Grgić Vretenca (Odonata) otoka Krka – ugroženost i status Dragonflies (Odonata) of the Krk Island – endangerment and status O‐96 105 I. Kurtek, M. Sudarić Bogojević, N. Turić, G. Vignjević, I. Vrućina, Ž. Zahirović, M. Bistrović, E. Merdić Sezonska dinamika komaraca na području županjske Posavine nakon poplave 2014. godine Seasonal dynamics of mosquitoes on the territory of Posavina near Županja after the 2014 flood 106 O‐97 E. Raspudić, M. Brmež, A. Budimir, Z. Pleša, J. Zdeličan Epitrix hirtipennis (Melsheimer, 1847) duhanov buhač, novi član entomofaune Hrvatske Epitrix hirtipennis (Melsheimer, 1847) tobacco flea beetle ‐ new member of Croatian entomofauna O‐98 107 T. Koren, S. Gomboc th
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Raznolikost noćnih leptira Strahinjščice i Ivanščice Diversity of moth fauna of Strahinjščica and Ivanščica 108 O‐99 M. Šag, N. Turić, Ž. Zahirović, B. Lauš, T. Koren, M. Zadravec Struktura saproksilnih kornjaša (Coleoptera) nizinskih šuma Saproxylic beetle (Coleoptera) assemblages in lowland forest O‐100 109 I. Vrućina, Ž. Zahirović, G. Vignjević, E. Merdić, A. Klobučar Vektor Aedes albopictus – Azijski tigrasti komarac u Osijeku, Hrvatska Vector Aedes albopictus ‐ Asian tiger mosquito in Osijek, Croatia Biologija kopnenih voda i kopna Biology of freshwater and terrestrial ecosystems IO‐3 110 M. Sabovljević, A. Sabovljević Bryophyte conservation biology in Europe ‐ insights into contemporary achievments, problems, solutions and management O‐101 111 M. Piria, T. Tomljanović, N. Šprem, D. Matulić, I. Aničić, R. Safner, T. Treer Promjene sastava ihtiofaune rijeke Save u Hrvatskoj tijekom posljednjeg desetljeća (2003.‐2013.) Ichthyofaunal changes of the River Sava, Croatia over last decade (2003‐2013) O‐102 112 M. Miliša, D. Đikić, I. Čolić, D. Grozić, T. Mandić Električna polja ‐ urbani pritisak na organizme u kopnenim vodama Electric fields ‐ the urban pressure on freshwater organisms O‐103 113 R. Matoničkin Kepčija, M. Miliša, M. Ivković, Z. Mihaljević Utjecaj hidrološkog stresa na obraštaj u NP Krka Influence of hydrological stress on periphyton in Krka National park O‐104 114 M. Ivković, M. Miliša, V. Baranov, Z. Mihaljević Zakrivenost vegetacijom kao glavni pokretač strukture zajednice izvorskih dvokrilaca Canopy coverage as a main driver in structuring spring Diptera assemblages O‐105 115 J. Lajtner, I. Zrinščak, M. Špoljar, T. Dražina, I. Radanović, B. Črgar, A. Ostojić Raznolikost staništa litoralne zone kao odrednica sastava zajednice puževa u plitkom eutrofnom jezeru Habitats heterogeneity of the littoral zone in the determination of snail assemblages in the shallow eutrophic lake O‐106 116 L. Vidatić, M. Ljuština, I. Ivanek, D.Lisičić Istraživačka aktivnost dinarskog voluhara (Dinaromys bogdanovi Martino, 1922) th
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Exploratory activity of Martino's snow vole (Dinaromys bogdanovi Martino, 1922) 117 O‐107 M. Kovač, M. Ljuština, D. Hamidović, M. Bračić, D. Lisičić Prvi podaci o akustičnom repertoaru dinarskog voluhara (Dinaromys bogdanovi) u zatočeništvu First data on the acoustic repertoire of Balkan snow vole (Dinaromys bogdanovi) in captivity Toksikologija i ekotoksikologija Toxicology and ecotoxicology 118 IO‐4 B. K. Hackenberger Kvalitativni pristup ekotoksikološkim istraživanjima – formalna potreba ili nužnost The qualitative approach in ecotoxicological studies – a formal need or a necessity O‐108 119 O. Jovanović, B. K. Hackenberger, Ž. Lončarić, M. Velki, G. Palijan Utjecaj pesticida na žablja jaja Effects of pesticides on frogs' eggs O‐109 120 S. Hayretdağ, B. Erkmen, E. Taşcı, F. Erkoç Spermiotoxicity and embryotoxicity of esbiothrin in sea urchin Paracentrotus lividus using short‐ term marine toxicity larval development methods O‐110 121 A. Oršolić, D. K. Hackenberger, Ž. Lončarić, V. Feigl, B.K. Hackenberger Dinamika rasta algi na medijima s dodatkom crvenog mulja Algae growth dynamics on medium suplemented with red mud O‐111 122 J.P. Bourdineaud, M. Šrut, A. Štambuk, M. Tkalec, D. Brèthes, K. Malarić, G. Klobučar Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms O‐112 122 B. Hamer, A. Baričević, N. Medić, V. S. Perusco Analiza PAH metabolita u žuči odabranih komercijalnih riba duž istočne Jadranske obale Bile PAH metabolites content in several commercial fishes along eastern Adriatic coast O‐113 123 Lj. Prester, J. Macan Alergeni grinja, žohara i plijesni u sedimentiranoj prašini u kućanstvu u Hrvatskoj Allergens of mites, cockroaches and moulds in settled dust in housholds in Croatia O‐114 124 J. Krasić, D. Đikić Sinergistički učinci vazodilatacijskih i antikoagulacijskih lijekova na oksidativni stres u mozgu Synergistic effects of vasodilatational and anticoagulative drugs on brain oxidative stress th
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Mikrobiologija Microbiology 126 O‐115 M. Šeruga Musić, A. Slović, M. Pinterić Komparativna analiza genoma fitoplazmi: od nestabilnosti genoma do prilagodbe na domaćine Comparative analysis of phytoplasma genomes: from genome instability to host adaptation O‐116 127 D. Škorić, M. Šeruga Musić, S. Černi Molekularna tipizacija uzročnika filodije uljane repice Molecular typing in deciphering etiology of rape phyllody disease O‐117 128 V. Karin, M. Ježić, M. Idžojtić, M. Ćurković Perica Raznolikost gljive Cryphonectria parasitica u zarastajućim kalusima kestena Diversity of Cryphonectria parasitica in healed chestnut cankers O‐118 129 L. Nuskern, Z. Katanić, Lj. Krstin, M. Ježić, M. Ćurković‐Perica Opstojnost virusa Cryphonectria hypovirus 1 može ovisiti o njegovom prijenosu između različitih tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica Persistence of Cryphonectria hypovirus 1 in Cryphonectria parasitica might be affected by the transfer between the different vegetative compatibility types of the host Komparativna fiziologija, imunobiologija i biologija čovjeka Comparative physiology, immunobiology and human biology IO‐5 130 R. Karlić, P. Polak, A. Koren, R. Thurman, R. Sandstrom, M.S. Lawrence, A. Reynolds, E. Rynes, K. Vlahoviček, J.A. Stamatoyannopoulos, S. R. Sunyaev Predviđanje ishodišnih stanica raka na temelju organizacije kromatina Using chromatin organization to predict cancer cell‐of‐origin O‐119 132 V. Tadić, P. Bašić Palković, B. Sasi, M. Klasić, P. Korać Replikacija u B‐staničnim limfomima Replication in B‐cell lymphoma O‐120 133 V. Vuković, M. Acman, M. Klasić, P. Dobrinić, P. Korać Razvoj testova za analizu metilacije DNA regulatornih regija gena GMNN i EZH2 i njihova primjena u istraživanju podtipova difuznog B‐velikostaničnoga limfoma Assay development for DNA methylation analysis of regulatory regions of genes GMNN and EZH2 and their application in evaluation of diffuse large B‐cell lymphoma subtypes O‐121 134 R. Žunec Histokompatibilnost i imunogenetika u transplantaciji solidnih organa u Hrvatskoj Histocompatibility and immunogenetics in solid organ transplantation in Croatia th
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135 O‐122 Z. Grubić Histokompatibilnost i imunogenetika u alogenoj transplantaciji krvotvornih matičnih stanica u Hrvatskoj Histocompatibility and immunogenetics in allogeneic hematopoietic stem cell transplantation in Croatia O‐123 136 H. Lepeduš, A. Kurtović, I. Jozić, D. Gašo Sokač, I. Marčinko, V. Cesar Akutni utjecaj kombiniranog tretmana dijafragmalnog disanja i progresivne mišićne relaksacije na antioksidacijsku učinkovitost sline u mladih odraslih ljudi povezan s njihovim crtama osobnosti Acute responses of saliva antioxidant capacity to combined diaphragmatic breathing and progressive muscle relaxation treatments in young adults related to their personality traits O‐124 137 V. Šlipogor, T. Gunhold‐de Oliveira, J.J.M. Massen, Z. Tadić, T. Bugnyar Monkeys with personality: consistent inter‐individual differences in common marmosets (Callithrix jacchus) O‐125 138 L. Vranković, I. Delaš, S. Reljić, Đ. Huber, J. Kusak, K. Klobučar, G. Krivić, D. Octenjak, N. Mikinac, Z. Stojević, J. Aladrović Usporedba masnokiselinskog sastava potkožnog masnog tkiva smeđeg medvjeda (Ursus arctos) i sivog vuka (Canis lupus) Comparison of fatty acid composition of subcutaneous adipose tissue of brown bear (Ursus arctos) and gray wolf (Canis lupus) POSTERSKA PRIOPĆENJA / POSTER PRESENTATIONS Konzervacijska biologija, zaštita prirode i okoliša Conservational biology, nature and environment protection P‐1 141 I. Boršić, V. Posavec Vukelić, T. Kirin Kockavica (Fritillaria meleagris L., Liliaceae) – 10 godina akcije praćenja stanja za osnovne i srednje škole Snake's head fritillary (Fritillaria meleagris L., Liliaceae) – 10 years of monitoring campaign for primary and secondary schools P‐2 142 A. Đanić, Z. Mesić, M. Škunca, A. Popijač, E. Patčev, S. Kapelj, J. Križan Definiranje ekoloških ciljeva u skladu s ekološkom mrežom Natura 2000 za potrebe revitalizacije Erdutsko‐daljskog dunavca Defining ecological objectives according to ecological network Natura 2000 for the purpose of revitalization of “Erdut‐Dalj dunavac” P‐3 143 M. Gubić, M. Baričević, D. Fantina, A. Mojsović Ćuić Suživot ljudi i prirode na primjeru Nacionalnog parka Plitvička jezera – je li isto moguće? th
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Coexistence of local population and nature in the example of the Plitvice Lakes National Park – is it possible? 144 P‐4 G. Gužvica, I. Bošnjak, A. Bielen, D. Babić, B. Radanović‐Gužvica, L. Šver Analiza metoda za praćenje prijelaza divljih životinja preko zelenih mostova Analysis of methods for wildlife monitoring on green bridges P‐5 145 G. Gužvica, I. Gabrić, K. Protrka, Z. Grgurić, L. Šver Preliminarni rezultati istraživanja vukova u Parku prirode Biokovo The research of wolves in the Nature Park Biokovo – preliminary results P‐6 146 S. Hudina, M. Samardžić, A. Lucić, M. Jelić, I. Maguire Nova strana vrsta deseteronožnih rakova (mramorni rak (Procambarus fallax (Hagen, 1870) f. virginalis) u slatkovodnim ekosustavima Hrvatske New non‐indigenous crayfish (the marble crayfish (Procambarus fallax (Hagen, 1870) f. virginalis) in Croatian freshwaters P‐7 147 J. Kusak, Đ. Huber, N. Trenc, J. Jeremić, S. Desnica Smjernice za procjenu utjecaja vjetroelektrana na velike zvijeri Guidelines for assessment of wind farms impact on large carnivores P‐8 148 Ž. Lončarić, M. Jambrović, B. K. Hackenberger Modificirani Leslie‐Lefkovitch genetički algoritamski modelski sustav dinamike populacija komaraca Modified Leslie‐Lefkovitch‐genetic algorithm hybrid models system of mosquito population dynamic P‐9 149 M. Mazija, Z. Domazetović, B. Benčina, A. Štefan, Ž. Drdar Šišmiši na Brijunima – rezidencijalna elita ili masovni turizam? Bats on Brijuni – residential elite or mass tourism? P‐10 150 A. Mikuška, A. Felja, D. Bjedov, K. Kajan, M. Blažević, A. Zovko, P. Benić Rasprostranjenost i trend gnijezdeće populacije vrane gačac (Corvus frugilegus L. 1758) na području Osijeka Distribution and trend of breeding population of rooks (Corvus frugilegus L. 1758) in Osijek P‐11 151 A. Mikuška, L. Begović, I. Mihić, K. Kljajić, R. Grgac Optimizacija metoda za molekularno određivanje spola kod čaplji (Ardeidae) Optimization of methods for molecular sex typing in herons (Ardeidae) P‐12 152 S. Novak Agbaba, I. Franić Biološka zaštita pitomog kestena gljivama antagonistima Biological control of sweet chestnut with antagonistic fungi P‐13 153 N. Pavlus, M. Drakšić, D. Švaljek, M. Perčić, D. Šegvić, M. Vuković, B. Matošić, S. Blažević th
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Solarni park Kosore – utjecaji na okoliš i ekološku mrežu Solar park Kosore – environmental impact and Natura 2000 network 154 P‐14 N. Pavlus, M. Perčić, M. Drakšić, M. Vuković, S. Blažević, Ž. Varga, A. Ptiček, H. Čizmek Sidrište u uvali Mezuporat, otok Biševo – kako sačuvati ekološku mrežu? The anchorage in the Mezuporat bay, island of Biševo ‐ how to preserve Natura 2000 network? P‐15 155 V. Peršić, A. Kočić, J. Horvatić Kvaliteta vode u vodotocima poljoprivrednog, močvarnog i šumskog porječja Water quality of watercourses draining agricultural, wetland and forested catchments P‐16 156 I. Plavac, A. Duplić, K. Jelić, J. Radović, R. Topić, I.Ilijaš Ekološka mreža Natura 2000 ‐ što se promijenilo nakon biogeografskog seminara? Natura 2000 network ‐ what has changed after the biogeographical seminar? P‐17 157 I. Sabolić, V. Dorić, A. Dobrović, A. Orlović, P. Crnčan, M. Rezo Istraživanje rijetkih i ugroženih svojti malakofaune Istre Research of rare and endangered taxa of Istrian malacofaune P‐18 158 M. Sabolić, I. Buj, M. Mrakovčić, M. Ćaleta Struktura i vijabilnost populacije srne, Capreolus capreolus u kontinentalnoj Hrvatskoj Structure and viability of the Roe deer Capreolus capreolus population in the continental Croatia P‐19 159 M. Sabolić, T. Čuković, A. Duplić, P. Lunko, D. Schneider Ocjena programa radova održavanja u području zaštite od štetnog djelovanja voda u Hrvatskoj ‐ uvjeti i mjere zaštite prirode: temelji, izazovi i planovi za budućnost Assessment of the programme for maintenance works for protection against harmful effects of water in Croatia – nature protection conditions and measures: fundamentals, challenges and plans for the future P‐20 160 R. Skukan, N. Koletić, L. Derežanin, N. Malešević, R. Španić, S. Dekanić Drvene obaloutvrde: ekološka alternativa kamenu i betonu na obalama vodotoka Tree revetments: ecological alternative to stone and concrete at the river bank 2. Simpozij edukacije biologije nd 2 Biology education symposium 161 P‐ 21 S. Fabac, V. Fabijanić Primjena online tečaja za učenje biologije u srednjoj školi Application of on‐line seminar for studying of biology in high school P‐22 162 N. Kletečki, D. Novoselić th
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Usvojenost osnovnih koncepata iz botanike kod učenika petog razreda osnovne škole Acquisition level of basic botany concepts by elementary school students in the fifth grade 163 P‐23 K. Latin, E. Merdić, M. Ništ, I. Labak Usvojenost nastavnog sadržaja primjenom konceptualnih mapa kod učenika srednje škole Adoption of teaching content using conceptual maps among secondary school students P‐24 164 M. Mihalić, H. Kostadinović Utjecaj kognitivnih razina zadataka na vjerodostojnost procjene usvojenosti koncepata genetike kod gimnazijskih učenika Impact of cognitive levels of tasks on genetics concepts assessment credibility in high school students P‐25 165 M. Rašan, S. Mikulić, D. Sumpor, M. Labaš Horvat Korištenje lokalnih resursa u terenskoj nastavi biologije Using local resources in biology field work P‐26 166 J. Razlog‐Grlica, N. Kletečki, M. Novosel Igra u ponavljanju nastavnih sadržaja vezanih uz životne zajednice mora The play in repeating the educational contents about marine life P‐27 167 Š. Romštajn‐Burchards, I. Bogut, Ž. Popović Ekološke teme u udžbenicima Republike Hrvatske i Savezne Republike Njemačke Environmental issues in textbooks of Croatian and Federal Republic of Germany P‐28 168 M. Srkoč, I. Štefančić, R. Halapir Franković Učeničko poznavanje životinja Hrvatske Student`s knowledge of animals that live in Croatia P‐29 169 A. Šarić, M. Marić Stavovi učitelja o biološkim nastavnim sadržajima u osnovnoj školi Teachers' attitudes toward the biology curriculum contents in elementary schools Evolucija, sistematika, filogenija i biogeografija Evolution, systematics, phylogeny and biogeography P‐30 170 V. Baković, M. Schebeck, C. Stauffer, G. Ragland, H. Schuler The potential role of ecological adaptation and Wolbachia infection in the diversification of Rhagoletis cerasi P‐31 171 N. Bilić‐Dujmušić, B. K. Hackenberger Primjena suvremenih tehnika prostorne statistike u ekološkim istraživanjima th
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Application of modern spatial statistical techniques in ecological research 172 P‐32 M. Jelić, F. Grandjean, M. Govedič, G. Klobučar, D. Guyonnet, I. Maguire Genetska struktura bjelonogog raka (Austropotamobius pallipes kompleks) otkriva moguću povezanost između tri krške rijeke tijekom geološke prošlosti Genetic structure of the white‐clawed crayfish (Austropotamobius pallipes complex) revealed possible historical connections between three karstic rivers P‐33 173 L. Jovović, B. Bruvo Mađarić, V. Mičetić Stanković, M. A. Jäch, M. Kučinić Filogenija i filogeografija roda Elmis (Coleoptera, Elmidae) krških tekućica jugoistočne Europe Phylogeny and phylogeography of genus Elmis (Coleoptera, Elmidae) in karstic running waters in southeastern Europe P‐34 174 N. Kević, S. Radić Brkanac, P. Peharec Štefanić, F. Faraguna, M. Kalafatić, G. Kovačević, D. Franjević Simbioza hidre i alge: karakterizacija endosimbiotskih zelenih algi izoliranih iz različitih sojeva zelenih hidri pomoću elektronske mikroskopije, morfometrijske i enzimske analize Hydra – algae symbiosis: characterization of endosymbiotic green algae isolated from different green hydra strains using electron microscopy, morphometric and enzyme analysis P‐35 176 D. K. Hackenberger, B. K. Hackenberger, D. K. Hackenberger ErIK: softverska platforma za identifikaciju gujavica Hrvatske ErIK: a software platform for identification of earthworms of Croatia P‐36 177 M. Ruščić, M. Šeput Flora otočića Šoltanskog arhipelaga The flora of Šolta archipelago islets P‐37 178 N. Ževrnja, B. Mitić, D. Vladović Analiza dijela herbarija Carla Studniczke (ord. Caprifoliaceen, Lonicereen i Stellateen) The analysis of part of Carl Studniczka's herbarium (ord. Lonicereen, Caprifoliaceen and Stellateen) 1. Balkanski herpetološki simpozij u suorganizaciji s Hrvatskim herpetološkim društvom st 1 Balkan herpetological symposium in co‐organization with Croatian herpetological society P‐38 179 S. Baškiera, M. Vucić, D. Jelić Populacijska ekologija i spolni dimorfizam livadne gušterice Lacerta agilis, Linnaeus 1758 s Dinare u Hrvatskoj Population ecology and sexual dimorphism in population of the sand lizard, Lacerta agilis, Linnaeus 1758 on Dinara mountain, Croatia P‐39 180 S. Baškiera, M. Vucić, B. Lauš, I. Vilaj Doprinos poznavanju herpetofaune Dinare u Hrvatskoj i bliže okolice Contribution to the knowledge of herpetofauna of Croatian part of Dinara mountain and its vicinity th
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181 P‐40 M. Cipot, A. Lešnik, K. Poboljšaj Is population of Moor frog (Rana arvalis) under severe decline in Mura river basin in Slovenia? P‐41 182 A. Ćurić, A. Zimić, D. Jelić Herpetofauna of Posavina region in Bosnia and Herzegovina and Croatia P‐42 182 S. Drakulić, H. Feldhaar, D. Lisičić, M. Mioč, I. Cizelj, M. Seiler, T. Spatz, M.‐O. Rödel Effect of environmental temperature on body condition and jumping performance of two geographically distant Rana temporaria populations P‐43 183 A. Dyugmedziev, N. Tzankov, G. Popgeorgiev Spatial replacement of close relatives, a passionate story of a mountainous population of Vipera ammodytes P‐44 183 D. Hlavati, M. Zadravec Faunistički nalazi vodozemaca i gmazova u okolici Našica Faunistic records of amphibians and reptiles in the Našice area P‐45 185 D. Jelić, M. Jelić, M. Futo, B. Halpern, K. Ljubisavljević, N. Tzankov Molekularna filogenija ivanjskog rovaša (Ablepharus kitaibelii) na središnjem i sjevernom dijelu područja rasprostranjenosti Molecular phylogeny of the Snake‐eyed Skink (Ablepharus kitaibelii) on the central and north part of its distribution range 186 P‐46 K. Koller, P. Konrad Kovač Novi dokazi o postojanju trogloksenih prilagodbi herpetofaune Dinarskog krša New evidence to existence of trogloxene adaptations in herpetofauna of Dinaric karst 187 P‐47 Y. Kornilev, F. Brischoux Coastal population of Dice snakes (Natrix tessellata) helps clarify the evolution of euryhaline physiology in marine tetrapods P‐48 187 Y. Kornilev, S. Etem, N. Natchev, I. Ivanchev, N. Tzankov, T. Ignatova‐Ivanova A preliminary study on the bioactivity of the microbiota in the post‐caecal GI tract of three Testudo species (Testudinidae, Testudines) with emphasis on lactic acid bacteria P‐49 188 P. Lisičar, B. Lauš, I. Vilaj, D. Jelić Morfološke značajke šare poljarice, Hierophis gemonensis (1768, Laurenti) Distribution and morphology of Balkan whip snake, Hierophis gemonensis (1768, Laurenti) P‐50 189 M. Lukač, E. Prukner‐ Radovčić, D. Horvatek, I. Cizelj, F. Martinković, D. Jelić, Ž. Gottstein th
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Praćenje zdravstvenog stanja čovječje ribice (Proteus anguinus) u zatočeništvu Monitoring of health status of the olm (Proteus anguinus) in captivity 190 P‐51 N. Lukić, J. Friščić, G. Šukalo, R. Dekić, B. Kukavica‐Jovanović Isoenzyme profiles of superoxide dismutase and catalase in liver and muscle selected anuran species P‐52 191 S. Mekinić, N. Ževrnja, J. Boban, G. Piasevoli, D. Vladović Zmije u herpetološkoj zbirci Prirodoslovnog muzeja u Splitu, Hrvatska Snakes in herpetological collection of the Natural history museum in Split, Croatia P‐53 191 N. Natchev, S. Handschuh, S. Lukanov, N. Tzankov, B. Naumov, I. Werneburg Discovery of an amphikinetic amphibian skull and its linkage to neotenic lifestyle P‐54 192 V. Šoltić, O. Jovanović, D. Jelić “Zamke smrti“ ‐ neprikladni građevinski zahvati u zaštićenim područjima mogu uzrokovati visoku smrtnost vodozemaca i gmazova “Death traps”‐ inappropriate constructions in protected areas can cause high mortality in amphibians and reptiles P‐55 193 G. Šukalo, S. Đorđević, Lj. Tomović Sexual dimorphism, diet and reproduction of the grass snake (Natrix natrix) in the region of the marshy‐pond ecosystem Bardača (Republic of Srpska, Bosnia and Herzegovina) P‐56 194 E. Šunje, F. Pasmans, Z. Maksimović, A. Martel, M. Rifatbefović Detection of continued annual mortality in the vulnerable alpine salamander, Salamandra atra prenjensis (Mikšić, 1969) not associated with the presence of known amphibian pathogens P‐57 195 A. Urošević, K. Ljubisavljević, A. Ivanović Preliminary assessment of the lacertid skull modularity – a case study of Podarcis muralis P‐58 195 I. Vilaj, B. Lauš, D. Jelić Riđovka, Vipera berus (Squamata, Viperidae) u Hrvatskoj: populacijska ekologija, odabir mikrostaništa i termoregulacija Common adder, Vipera berus (Squamata, Viperidae) in Croatia: population ecology, microhabitat preferences and thermoregulation P‐59 196 M. Zadravec Gmazovi i vodozemci Vugrovečke okolice Reptiles and amphibians of the Vugrovec area P‐60 197 M. Zadravec, R. Cesarec, Z. Tadić Gmazovi i vodozemci kamenoloma Bizek th
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Reptiles and amphibians of the Bizek quarry 200 P‐61 A. Zimić, D. Jelić, S. Lelo, M. Čengić New revision of the checklist of amphibian fauna in Bosnia and Hezegovina Biologija mora Marine biology P‐62 199 J. Antolović, E. Coppola, L. Bundone, S. Žalac, D. Škalić, V. Stinga Perusco, G. Hernandez Milan Uvidi o ekologiji prehrane sredozemne medvjedice Insights on the foraging ecology of the Mediterranean monk seal P‐63 200 M. Batistić, R. Garić Brooksia lacromae sp. nov. (Tunicata, Thaliacea), nova vrsta dvootvorke u Jadranskom moru Brooksia lacromae sp. nov. (Tunicata, Thaliacea), a new species of salp from the Adriatic sea P‐64 201 J. Boban, D. Vladović Zbirka rakova Prirodoslovnog muzeja u Splitu Natural history museum crustacean collection in Split P‐65 201 S. Bosak, L. Šupraha, D. Nanjappa, W. Kooistra, D. Sarno Morfologija i filogenija roda Bacteriastrum (Bacillariophyta) u Jadranskom moru Morphology and phylogeny of the genus Bacteriastrum (Bacillariophyta) in the Adriatic sea P‐66 202 M. Despalatović, I. Cvitković, A. Žuljević Sastav makrozoobentoskih beskralješnjaka u obraštajnim zajednicama u luci Split (Hrvatska, Jadransko more) Composition of macrozoobenthic invertebrates in fouling communities in the harbour of Split (Croatia, Adriatic sea) P‐67 203 N. Jasprica, D. Hafner, A. Car, T. Kapetanović Taksonomski sastav morskih bentoskih dijatomeja i njegov odnos s glavnim ekološkim čimbenicima u Bosni i Hercegovini Taxonomic composition of marine benthic diatoms in the relation to some key environmental variables in Bosnia and Herzegovina P‐68 204 P. Kružić, P. Rodić, A. Popijač, K. Vučković, P. Ankon, V. Matas, K. Cetinić, S. Sviben, A. Kovačev, N. Moraj Dubinska rasprostranjenost koralja u koraligenskoj biocenozi istočnog Jadrana Depth distribution of corals in the coralligenous of the eastern Adriatic sea P‐69 205 M. Marić, M. De Troch, S. Olenin th
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Trofičke interakcije u zaštićenim morskim područjima: uloga stranih invazivnih vrsta Trophic interactions in marine proteced areas: the role of invasive alien species 206 P‐70 S. Matić‐Skoko, J. Ferri, N. Stagličić, P. Tutman, D. Brajčić Jurica Dnevni prirasti otolita kao metoda validacije točnog određivanja starosti trlje od kamena, Mullus surmuletus Daily otolith increments as validation method for age determination of the striped red mullet, Mullus surmuletus P‐71 207 J. Miočić‐Stošić, G. Pleslić, N. Rako Gospić, C.M. Fortuna, D. Holcer Procjena brojnosti zajednice dobrih dupina (Tursiops truncatus) Viškog akvatorija Abundance estimates of the bottlenose dolphin (Tursiops truncatus) community in Vis archipelago P‐72 208 B. Mustać Stanje populacije inćuna (Engraulis encrasicolus L. 1758) u istočnom dijelu Jadranskog mora Anchovy (Engraulis encrasicolus L. 1758) population status in the eastern Adriatic sea P‐73 209 D. Pavičić‐Hamer Praćenje radioekološkog stanja sjevernog Jadrana korištenjem dagnje Mytilus galloprovincialis kao indikatora Radioecology monitoring of the northen Adriatic sea using mussels Mytilus galloprovincialis as an inidcator P‐74 210 G. Pleslić, A. Impetuoso, C. Fortuna Pojavnost i gustoća dobrih dupina (Tursiops truncatus) u Murterskom moru Occurrence and density of bottlenose dolphins (Tursiops truncatus) in Murter sea P‐75 211 I. Radonić, I. Cvitković, M. Despalatović Rasprostranjenost, abundancija i razmnožavanje vrste Siphonaria pectinata (Gastropoda, Pulmonata) u akvatoriju grada Splita Distribution, abundance and reproduction of the species Siphonaria pectinata (Gastropoda, Pulmonata) in the Split city aquatorium P‐76 212 G. Pleslić, G. Kett, N. Rako Gospić, T. Vučur, D. Holcer, J. Miočić‐Stošić, P. Mackelworth Utjecaj strategije hranjenja na društvenu strukturu dobrih dupina (Tursiops truncatus) u istočnom Jadranu, Hrvatska Influence of foraging strategies on social structure of bottlenose dolphins (Tursiops truncatus) in eastern Adriatic sea, Croatia P‐77 213 N. Rako Gospić, G. Pleslić, S. Stipić, J. Corbin, M. Picciulin Povezanost podvodne buke u cresko – lošinjskom akvatoriju sa promjenama u glasanju dobrih dupina (Tursiops truncatus) Shifts in bottlenose dolphin (Tursiops truncatus) vocalization in relation to sea ambient noise in the Cres and Lošinj waters, Croatia th
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P‐78 S. Vrgoč, N. Ževrnja Malakološka zbirka Petra Novaka Malacological collection of Petar Novak 6. Simpozij Hrvatskog društva za biljnu biologiju 6th Symposium of the Croatian Society of Plant Biologists
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P‐79 216 M. Curman, M. Tušek, M. Babić, M. Tkalec Fotosintetska učinkovitost, udio fotosintetskih pigmenata i fenolnih spojeva te antioksidacijska aktivnost u rosika (Droseraceae) pri različitim uvjetima uzgoja Photosynthetic efficiency, content of photosynthetic pigments and phenolic compounds, and antioxidative activity in Droseraceae at different growth conditions
P‐80 D. Leljak‐Levanić, N. Bauer, S. Mihaljević Zajedničke razvojne značajke somatske i zigotne embriogeneze Somatic and zygotic embryos share common developmental features
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P‐81 218 N. Kletečki, T. Vujnović, E. Kletečki, M. Vuković Rasprostranjenost drvenastih invazivnih vrsta Robinia pseudoacacia L., Reynoutria japonica Houtt. i Acer negundo L. na području Samobora Distribution of non‐herbaceous invasive species Robinia pseudoacacia L., Reynoutria japonica Houtt. and Acer negundo L. in the Samobor area
P‐82 K. Majsec, M. Pavlica Uloga transportera HMA u akumulaciji Cd u vrsti Nicotiana glauca Role of HMA transporters in Cd accumulation in Nicotiana glauca
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P‐83 220 I. Šola, B. Majić, S. Likić, I. Juranović Cindrić, G. Rusak Hranidbena vrijednost kore, plodova i sjemenki oskoruše (Sorbus domestica L.): minerali i fenolni spojevi Nutritional value of Sorbus domestica L. bark, fruits and seeds: minerals and phenolics
P‐84 221 M. Tušek, M. Curman, M. Babić, M. Tkalec Fotosintetska učinkovitost, udio fotosintetskih pigmenata i fenolnih spojeva te antioksidacijska aktivnost u različitim dijelovima vrčeva u tri hibrida roda Sarracenia Photosynthetic efficiency, photosynthetic pigments, and phenolic compounds, and antioxidant activity in different pitcher parts of three Sarracenia hybrids
P‐85 222 V. Vujčić, S. Radić Brkanac, M. Radić Stojković, S. Ivanković, J. Hrenović, M. Ruščić, B. Pevalek‐Kozlina Antioksidacijska, antibakterijska i citotoksična aktivnost vodenog ekstrakta dubrovačke zečine (Centaurea ragusina L.) Antioxidant, antibacterial and cytotoxic activities of water extract of plant species (Centaurea ragusina L.) th
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P‐86 M. Vujičić, M. Sabovljević, M. Ćosić, V. Maksimović, D. Mišić, A. Sabovljević Do sugar contents in selected moss species imply to their salt resistance?
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P‐87 224 M. Zovko, P. Cvjetko, I. Vinković Vrček, B. Balen Učinak nanočestica srebra na pojavu oksidacijskog stresa u klijancima duhana (Nicotiana tabacum) The effect of silver nanoparticles on oxidative stress in tobacco seedlings (Nicotiana tabacum) 3. Simpozij Hrvatskog entomološkog društva 3rd Symposium of the Croatian Entomology Society P‐88 225 M. Bistrović, N. Turić, M. Temunović, G. Vignjević, E. Merdić Usporedba učinkovitosti metoda uzorkovanja vodenih kukaca (Heteroptera i Coleoptera) s obzirom na atraktant, veličinu vrsta i stanište A comparison of methods for sampling aquatic insects (Heteroptera and Coleoptera) considering the bait, body size and habitat type
P‐89 226 N. Landeka, I. Čupić Rasprostranjenost crne udovice (Latrodectus tredecimgutattus, Rossi 1790.) na području Hrvatske i BIH Distribution of the black widow spider (Latrodectus tredecimgutattus, Rossi 1790.) in the area of Croatia and Bosnia and Herzegovina
P‐90 J. Skejo Fauna skakavaca (Insecta: Orthoptera: Caelifera) Hrvatske Croatian short‐horned grasshoppers' (Insecta: Orthoptera: Caelifera) fauna
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P‐91 A. Štih, T. Koren Raznolikost vretenaca Hrvatskoga zagorja Diversity of dragonfly fauna of Hrvatsko zagorje
P‐92 229 I. Vrućina, A. Previšić, E. Merdić Nove vrste tulara (Insecta: Trichoptera) za faunu Hrvatske iz parka prirode Kopački rit New species of caddisflies (Insecta: Trichoptera) for Croatian fauna from the Kopački rit nature park Biologija kopnenih voda i kopna Biology of freshwater and terrestrial ecosystems P‐93 230 D. Čerba, I. Turković Čakalić, V. Ergović, M. Koh, M. Lesjak, A. Petek, A. Vuković, I. Balković, Đ. Milošević, F. Stević Struktura zajednice i hranidbene navike slatkovodnih riba u plitkom jezeru umjerenog područja Community structure and feeding habits of freshwater fish in a shallow temperate lake 231 P‐94 I. Grigorszky, M. Gligora Udovič, I. Somlyai, B. Palombi, L. Czövek, Gy. Dévai, S. A. Nagy. th
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Relationships between algal production and the physical and chemical properties of a drinking water reservoir 231 P‐95 N. Jasprica, D. Hafner, A. Car, T. Kapetanović Taksonomski sastav morskih bentoskih dijatomeja i njegov odnos s glavnim ekološkim čimbenicima u Bosni i Hercegovini Taxonomic composition of marine benthic diatoms in the relation to some key environmental variables in Bosnia and Herzegovina P‐96 232 M. Jelić, T. Šarčević, K. Jelić, I. Katanović Rasprostranjenost i procjena veličine populacije vidre (Lutra lutra L.) u alpskoj biogeografskoj regiji Hrvatske Distribution and estimated population size of the otter (Lutra lutra L.) in the alpine biogeographical region of Croatia P‐97 233 D. Kiš‐Novak, M. Kerovec Biološko‐ekološka obilježja aluvijalnih potoka Međimurja Biological‐ecological features of alluvial streams of the region of Međimurje P‐98 235 M. Kučinić, G. Mesarić, I. Mihoci, E. Vasilić, M. Blažević, T. Koren, T. Finderle, Lj. Ladavac, M. Grgurev Vertikalna rasprostranjenost, faunističke i biogeografske značajke danjih leptira (Insecta, Lepidoptera) parka prirode Učka Vertical distribution, faunal and biogeographical features of butterflies (Insecta, Lepidoptera) from the Učka nature park P‐99 236 M. Ljuština, I. Katanović, M. Rezo, I. Ivanek, M. Damjanović, D. Lisičić Sezonske razlike u unosu, skladištenju hrane i težini dinarskog voluhara (Dinaromys bogdanovi) u zatočeništvu Seasonal differences in food intake, caching and body mass of the endemic balkan snow vole (Dinaromys bogdanovi) in captivity P‐100 237 M. Mejdandžić, N. Malešević, N. Koletić, M. Nemet, K. Kralj Borojević Fitobentos i vodeni makrofiti kao elementi biološke kvalitete i klasifikacije staništa duž subplaninske krške rijeke Phytobenthos and aquatic vegetation as biological quality and habitat description elements along submountain carstic river
P‐101 M. Mrakovčić, M. Ćaleta, Z. Marčić, D. Zanella, P. Mustafić Značake ihtiofaune rijeke Sutle Fish diversity of the river Sutla P‐102 D. Prlić Početna istraživanja vaskularne flore i staništa uz rijeku Muru u Hrvatskoj Initial research of vascular flora and habitats along the Mura river in Croatia
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240 P‐103 M. Rašan, L. Rašan, V. Pirc Mezga Staništa slatkovodnih meduza (Craspedacusta sowerbyi Lankester 1880.) na području donjeg Međimurja Freshwater jellyfish habitats in lower Međimurje (Craspedacusta sowerbyi Lankester 1880) P‐104 241 S. Smiljkov, D. Gusheski, V. Slavevska‐Stamenkovikj, R. Ilieska, K. Krstevska, S. Stojanovski Qualitative analysis of representatives from Annelida (Oligochaeta and Hirudinea) from Kumanovo bath P‐105 242 F. Stević, A. Milić, D. Špoljarić Maronić, T. Žuna Pfeiffer, V. Zahirović, M. Mihaljević Vertikalna distribucija morfoloških oblika vrste Cylindrospermopsis raciborskii u poplavnom jezeru Vertical distribution of morphological forms of Cylindrospermopsis raciborskii in a floodplain lake P‐106 243 I. Strbad, V. Mičetić Stanković, M. Rašan, M. Kučinić Antropogeni utjecaj na raznolikost bentičkih beskralježnjaka srednjeg toka rijeke Drave i pripadajućih šljunčara Anthropogenic impact on benthic macroinvertebrate diversity in the middle reach of Drava river and the accompanying gravel pits P‐107 244 B. Vlaičević, J. Vidaković Dinamika naseljavanja i sukcesije trepetljikaša u perifitonu jezera poplavnog područja Colonization and succession dynamics of periphytic ciliates in a floodplain lake P‐108 245 P. Žutinić, I. Petrić, M. Gligora Udovič, K. Kralj Borojević, I. Mikac, I. Bokan, I. Špoljarić, G. Mršić, J. Kamberović, A. Plenković‐Moraj, S. Gottstein, I. Ternjej Mikrobni biofilm periodičkog slatkovodnog krškog izvora Microbial mats in intermittent freshwater karstic spring Toksikologija i ekotoksikologija Toxicology and ecotoxicology P‐109 246 S. Babić, R. Sauerborn Klobučar, J. Barišić, G. Klobučar, N. Topić Popović, I. Strunjak‐Perović, R. Čož‐Rakovac Procjena utjecaja otpadnog mulja na okoliš – mogući biomarkeri Potential biomarkers for sewage sludge pollution monitoring P‐110 247 S. Babić, R. Sauerborn Klobučar, G. Klobučar, S. Kepec, H. Višić, J. Barišić, N. Topić Popović, I. Strunjak‐Perović, R. Čož Rakovac Procjena toksičnosti komunalnih otpadnih voda iz postrojenja za pročišćavanje otpadnih voda korištenjem testa embriotoksičnosti na zebricama Evaluation of toxic potential of municipal waste waters from wastewater treatment plant by zebrafish embryo toxicity test P‐111 248 G. Gül, A. Ç. Karasu Benli, F. Erkoç, A. Gül th
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Can histopathological investigation be a marker of pollution impacts? A case study of some economically important fish species in the euthrophic Lake Mogan, Central Anatolia 249 P‐112 K. Jurica, S. Šegan, D. Milojković Opsenica, I. Brčić Karačonji, V. Benković, N. Kopjar Procjena toksičnosti vodenog ekstrakta lista obične planike (Arbutus unedo L.) i arbutina na ljudskim limfocitima periferne krvi u uvjetima in vitro Toxicity assessment of strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes in vitro P‐113 250 A. Ç. Karasu Benli, S. Kayiran Tunca, P. Arslan, G. Gül, A. Sepici Dinçel Determination of acute and sublethal effects of permethrin on narrow clawed crayfish (Astacus leptodactylus Esch. 1823) P‐114 251 N. Kopjar, V. Kašuba, R. Rozgaj, M. Mladinić, M. Milić, D. Želježić Citotoksični, genotoksični i citogenetički učinci klorpirifosa na ljudske limfocite periferne krvi u uvjetima in vitro Evaluation of the cytotoxic, genotoxic and cytogenetic effects of chlorpyrifos in human peripheral blood lymphocytes in vitro P‐115 252 H. M. Kutlu , D. Vejselova, G. Kuş Determination of cell death mechanism on human lung adenocarcinoma cells exposed to ceranib‐2 P‐116 253 A. Maslać, M. Tkalec, M. Maslać Učinak kadmija na fotosintezu i sadržaj sekundarnih metabolita u lišajeva Parmelia sulcata, Evernia prunastri i Elavoparmelia caperata The impact of cadmium on photosynthesis and secondary metabolites in lichens Parmelia sulcata, Evernia prunastri and Elavoparmelia caperata P‐117 254 A. Perović, B. Šljukić, S. Perović, M. Šrut, G.I.V. Klobučar Pollution biomonitoring of the River Moraca and Lake Skadar using caged mussels Unio sp. P‐118 254 L. Ulm, I. Hrga, A. Krivohlavek Biološka razgradnja organskih tvari u vodi‐odabir metode HRN EN ISO 9439:2000 ili HRN EN ISO 10707:2000 Biological degradation of organic compounds in water‐selection of the method HRN EN ISO 9439:2000 or HRN EN ISO 10707:2000 P‐119 255 D. Vejselova, H. M. Kutlu, G. Kuş Investigation of ceranib‐2 induced cytotoxicity on human lung adenocarcinoma cells Mikrobiologija Microbiology P‐120 256 A.‐M. Ladiš, A. Mojsović Ćuić th
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Bez njih se ne može: eukariotski organizmi ‐ stanovnici aktivnog mulja We can't do without them: eucaryotic organisms – inhabitants of the activated sludge 257 P‐121 E. Žižić‐Gušo, N. Krstulović, S. Šestanović Heterotrofne bakterije u sedimentu šireg područja Splita Heterotrophic bacteria in sediment of a wider area of Split Komparativna fiziologija, imunobiologija i biologija čovjeka Comparative physiology, immunobiology and human biology P‐122 258 V. Balta, D. Odeh, M. Mataković, M. Kunštić, M. Kukolj, N. Oršolić Animalni model psorijaze: antiupalna i antioksidativna učinkovitost epigalokatehin galata Animal models of psoriasis: antiinflammatory and antioxidant epigallocatehin gallate efficiency P‐123 259 S. Blažević, M. Merkler, D. Hamidović, D. Hranilović Separacijska anksioznost u mladunaca štakora s perinatalno promijenjenim metabolizmom serotonina Separation anxiety in rat pups with perinatally altered serotonin metabolism P‐124 260 D. Đikić, R. Fuchs, S. Matić Skoko, D. Skaramuca, Z. Franić, I. Landeka, B. Skaramuca Razlike u sastavu masnih kiselina u murine, ugora i jegulje iz Jadranskog mora blizu Dubrovnika Differences in fatty acid composition between Moray, Conger and Common eel from Adriatic sea near Dubrovnik P‐125 262 M. Kukolj, M. Kunštić, R. Račan, E. Rođak, N. Oršolić Učinkovitost kafeinske kiseline na peritonealnu angiogenezu u Ehrlichovom ascitesnom tumoru The efficiency of caffeic acid on oxidative stress and angiogenesis in Ehrlich ascites tumor P‐126 263 M. Kunštić, M. Kukolj, N. Oršolić Galna kiselina smanjuje tumorski rast i angiogenezu u miševa s Ehrlichovim ascitesnim tumorom Gallic acid reduces tumor growth and angiogenesis in mice with Ehrlich ascites tumor P‐127 264 A. Ševo, S. Šogorić, P. Stanić, A. Horvat Knežević, V. Benković Povezanost životnih navika i primarnih oštećenja DNA spermija s reproduktivnom sposobnosti muškaraca Relationship between lifestyle habits and primary DNA damage of the sperm and reproductive ability of men P‐128 265 N. Oršolić, D. Đikić, J. Nemrava, P. Majcen, M. Kunštić, M. Kukolj, E. Rođak, E. Goluža Učinkovitost kvercetina na biomarkere za osteoporozu The effectiveness of quercetin on biomarkers for osteoporosis P‐129 266 N. Oršolić, M. Kunštić, M. Kukolj, I. Čolić th
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Inhibicija polarizacije makrofaga kafeinskom kiselinom: međuodnos ROS, antioksidansa i upalnih citokina Inhibition of macrophage polarization by caffeic acid: interplay between ROS, antioxidans, and inflammatory cytokines 267 P‐130 N. Oršolić, M. Kunštić, M. Kukolj, D. Sirovina Učinak flavonoida i hipertermalne intraperitonealne kemoterapije (HIPEC) na rast tumora i indukciju mikronukleusa u mišjem modelu tumora Effect of flavonoids and hyperthermal intraperitoneal chemotherapy (HIPEC) on tumour growth and micronucleus induction in mouse tumour model P‐131 268 N. Oršolić, M. Kunštić, M. Kukolj, D. Sirovina, I. Šopf Zaštitni i reparacijski učinak pentadekapeptida BPC 157 na stanice krvi, jetre i bubrega miša Protective and reparative effect of pentadecapeptide BPC 157 on mice blood, liver and kidney cells P‐132 269 N. Oršolić, D. Odeh, M. Mataković, V. Balta, M. Kunštić, M. Kukolj, K. Sašilo, D. Đikić Kvercetin smanjuje iritaciju kože induciranu heksil‐salicilatom ili di‐n‐propil disulfidom: oksidativni stres i upala Quercetin attenuates n‐hexyl salicylate or di‐n‐propyl disulfide‐induced skin irritation: oxidative stress and inflammation P‐133 270 D. Sirovina, N. Oršolić, M. Zovko Končić, G. Gregorović Naringenin ublažava patološke promjene u jetrima i bubregu miševa s dijabetesom Naringenin ameliorates pathological changes in liver and kidney of diabetic mice Genetika, stanična i molekularna biologija Genetics, cellular and molecular biology P‐134 272 I. Maguire, M. Jelić, G. Klobučar, A. Štambuk, M. Vucić, F. Grandjean Primjena analize mikrosatelitnih biljega na populacijama vrsta Astacus astacus i Astacus leptodactylus u Hrvatskoj ‐ preliminarni rezultati Application of microsatellite analysis on Astacus astacus and Astacus leptodactylus populations from Croatia ‐ preliminary results P‐135 273 M. Matešić, S. Jonjić, I. Jurak Istraživanje uloge mikroRNA kod herpes simpleks virusa 1 Investigating the roles of microRNAs in herpes simplex virus 1 infection P‐136 274 D. Perina, M. Korolija, M. Popović Hadžija, I. Grbeša, R. Belužić, M. Imešek, C. Morrowe, T. Bakran‐ Petricioli, A. Mikoč, H. Ćetković Karakterizacija gena/proteina FAU iz morske spužve Suberites domuncula Characterization of FAU gene and protein from marine sponge Suberites domuncula P‐137 275 D. Perina, A. Mikoč, J. Ahel, H. Ćetković, R. Žaja, I. Ahel th
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Distribucija proteina uključenih u poli(ADP‐ribozil)aciju u svim domenama života Distribution of protein poly(ADP‐ribosyl)ation systems across all domains of life 276 P‐138 M. Plazibat, A. Katušić Bojanac, K. Raković, N. Sinčić, Lj. Šerman, M.Vlahović, G. Jurić‐Lekić, F. Bulić‐Jakuš Valproat narušava rast štakorskog zametka i ektoplacentalnog stošca ex vivo Valproate impairs growth of the rat embryo and the ectoplacental cone ex vivo P‐139 278 I. Radonić, Ž. Trumbić, T. Šegvić‐ Bubić, L. Grubišić, I. Mladineo Genetska analiza populacije atlantske plavoperajne tune, Thunnus thynnus (Linnaeus, 1758) u kaveznom uzgoju primjenom mikrosatelitnih lokusa Genetic analysis of cage‐reared atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758) population by application of microsatellite loci P‐140 279 I. Tomljanović, K. Skube, D. Pavoković Ekspresija i identifikacija novih proteina djelomično neuređene tercijarne strukture (IDP) iz biljke uročnjak (Arabidopsis thaliana) nakon izlaganja abiotičkom stresu Expression and identification of novel intrinsically disordered proteins in Arabidopsis thaliana during abiotic stress P‐141 280 T. Vojvoda Zeljko, E. Šatović, M. Plohl Genomi školjkaša ukazuju na transpozicijsku mobilnost satelitnih ponavljanja Bivalve genomes suggest transposition‐driven mobility of satellite DNA repeats P‐142 281 I. Bošnjak, A. Bielen, M. Jaklič, M. Cvitanić, K. Sepčić, T. Simčič, J. Lajtner, S. Hudina, T. Vojvoda Zeljko, E. Šatović, M. Plohl Razlike u otpornosti na antropogeni stres između invazivne i nativne vrste školjkaša Differences in tolerance to anthropogenic stress between invasive and native bivalves P‐143 282 A. Zubković, I. Ratkaj, I. Jurak Ispitivanje uloge staničnih miRNA u replikaciji herpes simpleks virusa 1 Investigating the roles of host microRNAs in herpes simplex virus 1 infection Kazalo autora / Index of Authors 284 Sponzori / Sponsors 296 th
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PLENARNA PREDAVANJA
PLENARY LECTURES
Plenarna predavanja Plenary lectures PL‐1 PROTEIN INTERACTION NETWORKS IN HEALTH & DISEASE I. Štagljar Departments of Biochemistry and Molecular Genetics, Donnelly Centre, University of Toronto, Canada In the past decade, numerous comprehensive proteomics studies have allowed researchers to generate detailed protein‐protein interaction ('interactome') maps, providing an unparalleled global view of the interplay between the different systems within the cell, and greatly increasing our understanding of the physiological mechanisms involved in both normal and disease states. My lab is focused specifically on understanding how the interactions of membrane proteins contribute to cellular disease states at a systems level. Despite extensive proteomics research in the past decade, there is a lack of in‐depth understanding of protein networks associated with integral membrane proteins because of their unique biochemical features, enormous complexity and multiplicity. This is a major obstacle to understanding the biology of deregulation of integral membrane proteins which leads to numerous human diseases, and consequently hinders our development of improved and more targeted therapies to help treat these diseases. To address this problem, my lab has developed two unique technologies specifically suited for the study of full‐length integral membrane proteins in their natural cellular context; the classic Membrane Yeast Two‐Hybrid (MYTH) 1‐5 and the newly created Mammalian Membrane TwoHybrid(MaMTH)6. Our ultimate goal is to uncover a wealth of information about protein interactions for the majority of 'druggable' human membrane proteins, which should in turn greatly facilitate the discovery of new truths about diseases like cancer, schizophrenia, cystic fibrosis, hypertension and Parkinson’s disease. During my talk, I will discuss our recent findings indicating that the application of MaMTH to the human Epidermal Growth Factor Receptor (EGFR) resulted in the identification of Crk II protein as a novel interactor of oncogenic EGFR (L858R), and showed that CRKII promotes persistent activation of aberrant signaling in non‐ small cell lung cancer (NSCLC) cells. I will also illustrate how MaMTH is a powerful tool for investigating dynamic interactomes of human integral membrane proteins and why it promises significant contributions to therapeutic research. PL‐2 THE RISE AND FALL OF GENOME COMPLEXITY AND OTHER MACROEVOLUTIONARY STORIES R. Bakarić, T. Domazet‐Lošo Ruđer Bošković Institute, Zagreb, Croatia Recent advances in understanding of evolutionary life cycle of genes reveal that gain and loss of gene families is major factor in the evolution of genome composition. However, it is unclear how gene family birth‐and‐death rates fluctuate at the macroevoutionary scale. Consequently it is obscure how genome complexity changed during evolutionary time in different lineages. Here, using phylostratigraphic approach and newly developed bioinformatic toolkit, we measured gene family birth‐and‐death rates at the phylogenetic tree comprising more than 600 eukaryotic genomes. We found that, in terms of time, th
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genome reduction is predominant mode of evolution sporadically interrupted by the rapid bursts of gene family gain. These episodes of gene family gain correlate with the major macroevolutionary transitions. PL‐3 DIVERGENCE AND CONSTRAINT IN THE ORIGIN OF NEW SPECIES P. Nosil Royal Society of London, University of Sheffield, United Kingdom The origin of new species creates biological diversity and understanding species formation is thus a key goal in biology. In this talk, I will tackle the issue of why some populations that begin the speciation process diverge further than others, a phenomenon central to understanding diversification. Using a combination of theoretical modeling and empirical studies of plant‐feeding insects I will show how adaptation to different ecological environments generally promotes speciation. However, this process can be constrained or counteracted by numerous factors. Specifically, speciation can stall 'partway' before completion due to: an insufficient number of genetic differences underlying adaptive divergence, ecological shifts that are too modest to drive strong divergence, and selective processes that increase genetic mixing between populations. The origin of new diversity thus reflects a balance between these factors driving and constraining evolutionary divergence. PL‐4 SOCIO‐SCIENTIFIC ISSUES: A CHALLENGE FOR BIOLOGY TEACHERS A. Šorgo Faculty for Natural Sciences and Mathematics, University of Maribor, Maribor, Slovenia In many contemporary documents is addressed need for different kind of education, where outcome of such approach is a citizen who not only possess solid knowledge foundation but also is able to use achieved knowledge to solve real‐world practical or intellectual problems even after finished formal education. In documents underpinning education emphasis is given to development of skills like critical thinking, creativity, ability to solve problems and soft skills like ability to learn, to activate tacit knowledge or to work in groups. Real and yet unsolved challenge is how to organize instructions to achieve at least a part of listed goals balancing between well‐tested practices what worked in the past and enthusiasm of untested novelties. Because we cannot treat instructions and school activities as from the society‐isolated and value‐free systems, Biology teachers cannot avoid teaching and follow up classroom discussions of sensitive themes. Beside already 'traditional' themes like environmental education is nowadays in the middle of public interest biotechnology with themes like genetic engineering, genetically modified organisms (GMOs), food from GMOs, therapeutic and reproductive cloning, substitute maternity, cloning of human, impact of GMOs on human health, health of animals, and environment, etc. To cover the social and scientific aspects of such the term 'socio‐scientific issue' was coined. From the socio‐ scientific view, such issues are frequently controversial and, especially in cases when they become the subject of public interest debates, are often backed up with limited knowledge and strong attitudes. In educational practice, such public debates are reflected in the classroom, where teachers are challenged by complex issues, where proficiency in one th
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discipline is in most cases insufficient to answer students’ curiosity. When such a theme is assigned to a Biology teacher, probably the most important obstacle is that teachers often lack appropriate knowledge about the social side of the issue. PL‐5 PLANT RESPONSE TO ABIOTIC STRESS D. Bar‐Zvi Ben‐Gurion University, Beer‐Sheva, Israel Being sessile organisms that cannot relocate, plants are continuously exposed to the ever‐ changing environment. To accommodate to these changes, plants evolved intensive molecular, biochemical and physiological mechanisms to respond to hostile environments. These changes include a global change in the transcriptome and proteome. I will discuss three molecular examples for plant response to drought and salt stress: (a) Salt stress impairs chloroplast structure and affects this organelle’s gene expression and genome copy number. (b) Transcription factors are key players in reaching new steady states in transcript levels. The stress hormone, abscisic acid, regulated ABI4 transcription factor plays central role in root biology under stress and non‐stress conditions. ABI4 modulates the activity of the Na+ transporter HKT1;1 and controls lateral root formation. (c) The Ubiquitin‐ Proteasome System (UPS) is a major pathway for controlled protein degradation in all eukaryotes, including plants. Proteins descended for degradation are covalently marked by a short ubiquitin chain, by a three step pathway. The third step, is catalyzed by a large family of substrate specific ubiquitin‐ligases, is believed to be the modulated step in the decision of protein fate. We have identified new ubiquitin‐ligases involved in plant response to abiotic stress, and study their role in plant response to the environment. PL‐6 ANALYSES OF MORPHOLOGY, PHYSIOLOGY, AND MICROBIAL DIVERSITY AFTER A RECENT DIETARY SWITCH IN A LIZARD A. Herrel UMR 7179 C.N.R.S/M.N.H.N., Département d'Ecologie et de Gestion de la Biodiversité, Paris, France Although evolution is commonly considered a slow process, recent evidence has shown that organisms can show dramatic and measurable phenotypic responses after introductions to novel environments in relatively short time spans. We have previously shown how lizards (Podarcis sicula) have rapidly evolved differences in head morphology, bite strength, and digestive tract structure after experimental introduction onto a small island in the Adriatic Sea, Croatia. Despite the short time scale (36 years) since this introduction, the introduced lizards became omnivores and evolved caecal valves in the hindgut, a structure rarely observed in lizards. These changes in morphology and performance parallel those typically documented among species and even families of lizards in both the type and extent of their specialization. Here, we present novel data on 1) the morphology of the cranium and its muscles using µCT scanning and 3D geometric morphometric approaches, 2) the digestive physiology of the two populations, and 3) the diversity of the microbiomes in the hindgut using metagenomic sequencing approaches. th
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PL‐7 HOW MUCH ROOM IS LEFT FOR CONVENTIONAL BIOLOGY IN MODERN NANOSCALE AND OMICS RESEARCH D. Drobne Department of Biology, Biotechnical faculty, Ljubljana, Slovenia Traditional biologists were trained to work at a specific level of biological complexity, and/or to investigate a specific group organisms. But now, many biologists call themselves integrative biologists. Also academic units and study programs have changed their names to Integrative biology. What is 'Integrative Biology'? Integrative biology has different definitions. It could be defined as multidisciplinary/cross‐disciplinary research of biological systems. Multidisciplinarity means bringing together researchers from different, areas of expertise or using a diversity of techniques and approaches to address particular questions (Wake 2003; Joyce and Palsson 2006). Integrative approaches seek both diversity and incorporation. These approaches deal with integrations across all levels of biological organization, from molecules to the biosphere, and with diversity across taxa, from viruses to plants and animals. Many of the questions now being addressed by biologists require both reductionistic and incorporative elements to contribute to an answer to a larger problem. Biologists are coming to the realization that our ability to deal with questions of biological complexity would benefit from high throughput systems biology, referred to as '‐omics' technology. Integrated 'omics' approaches have created exciting opportunities for biological researches. High‐throughput studies of biological systems are rapidly accumulating a wealth of ‘omics’‐scale data. However, this abundance of information also presents many obstacle. The main one includes extraction of discernable biological meaning from multiple omics data sets. In the presentation, two examples on successful integration of conventional biological study, nanotoxicity data and omic approach in understanding the effects of engineered nanomaterials on model test organisms (Dapnia magna and Tetrachymena termophyla) are presented in detail. At present, one can conclude that, the omics technologies are likely to continue its expansion for all fields in biological but when integrating omic‐data with the traditional approaches the difficult problems on previously unprecedented scales and could be tackled. Apparently, it is all about integrating, in biology. Integrative biology. PL‐8 GLYCANS AS INTEGRATORS OF GENES AND ENVIRONMENT – AN OFTEN‐IGNORED LAYER OF BIOLOGICAL COMPLEXITY G. Lauc University of Zagreb Faculty of Pharmacy and Biochemistry Glycosylation is an essential posttranslational modification generated by a complex biosynthetic pathway comprising hundreds of glycosyltransferases, glycosidases, transcriptional factors, ion channels and other proteins. This process results in the creation of branched oligosaccharide chains, called glycans, which become integral part of proteins and significantly contribute to their structure and function. Nearly all proteins invented after the appearance of multicellular life are glycosylated and glycans are glycans are functionally relevant for nearly all processes at the multicellular level. Since glycans are created without the genetic template, alternative glycosylation creates an additional layer of structural complexity by combining genetic variability with past and present environmental factors. th
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Individual variability in glycome composition is very large, but glycosylation of an individual protein seems to be under strong genetic influence, with the heritability of the (for example) IgG glycome being up to 80%. Structural details of the attached glycans are of great physiological significance and many pathological conditions are associated with various types of glycan changes. Since the onset of genome wide association studies (GWAS), thousands of genetic loci have been associated with different diseases and traits. However, in the last few years, and particularly after recent publication of the results from the ENCODE project, it is becoming increasingly clear that GWAS studies are only a beginning of the understanding of complex human diseases. Hypotheses generated in these studies have to be put in the context of complex biology of life and a more elaborate approach that combines different ‘omics phenotypes is needed to understand disease mechanisms and perform patient stratification that transcends genomics. Glycomics, as by far the most complex epiproteomic modification, has an immense potential in this respect, which is only beginning to be investigated.
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USMENA PRIOPĆENJA
ORAL PRESENTATIONS
Konzervacijska biologija, zaštita prirode i okoliša Conservational biology, nature and environment protection O‐1 VRIJEDNOST FAUNISTIČKIH ISTRAŽIVANJA KAO TEMELJA ZA PLANIRANJE AKTIVNE ZAŠTITE PRIRODE ‐ PROJEKT INTEGRACIJE U EU NATURA 2000 (NIP) I. Sučić1, L. Katušić2 1 Ministarstvo zaštite okoliša i prirode, Zagreb, Hrvatska ([email protected]) 2 Odjel za divlje i udomaćene svojte i staništa, Državni zavod za zaštitu prirode, Zagreb, Hrvatska Projekt integracije u EU Natura 2000 (NIP projekt) je stručni projekt sektora zaštite prirode kroz koji se prikupljaju postojeći te novi podaci o rasprostranjenju vrsta iz odabranih prioritetnih taksonomskih skupina. Prvom fazom projekta predviđeno je prikupljanje literaturnih i muzejskih podataka za 13 taksonomskih skupina, a kroz drugu terensko prikupljanje recentnih podataka i laboratorijska analiza za 9 prioritetnih taksonomskih skupina. U okviru projekta izrađuje se i detaljna karta prirodnih i polu‐prirodnih ne‐šumskih staništa Hrvatske. Primjer skupine Plecoptera, prikazuju važnost faunističkih projekata za planiranje aktivne zaštite prirode. Prema Crvenom popisu obalčara (2007) u Hrvatskoj je bilo poznato 28 vrsta obalčara, a Državni zavod za zaštitu prirode je raspolagao s 152 nalaza obalčara. Obradom dostupnih literaturnih referenci i muzejskih nalaza, te terenskih istraživanja u 2014. i 2015. godini ukupno su prikupljeni podaci o 3979 nalaza za 110 vrsta. Ključne riječi: aktivna zaštita prirode, faunistička istraživanja, NIP projekt FAUNISTIC RESEARCH VALUES AS THE BASELINE FOR PLANNING ACTIVE NATURE CONSERVATION ‐ EU NATURA 2000 INTEGRATION PROJECT (NIP PROJECT) I. Sučić1, L. Katušić2 1 Ministry of Environmental and Nature Protection, Zagreb, Croatia ([email protected]) 2 Department for Wild and Domesticated Taxa and Habitats, The State Institute for Nature Protection, Zagreb, Croatia EU Natura 2000 Integration Project (NIP project) is a professional project of nature protection sector, through it will be gathered existing and new data about distribution of species of the selected priority taxonomic groups. The first phase of the project foretell gathering of literature and museum data for 13 taxonomic groups, and throughout the second gathering recent data and laboratory analysis for 9 priority taxonomic groups. As a part of the project it will be also made a detailed map of natural and semi‐natural non‐forest habitats of Croatia. Example on taxonomic group Plecoptera is showing the importance of faunistic projects in planning active nature protection. According to the Red list of stoneflies (2007) in Croatia it was known 28 stoneflie species, and State Institute for Nature Protection disposed with 152 findings of stoneflies. Analysing the available literature references and museum findings, and also field research findings in 2014 and 2015, a total of 3979 findings for 110 species were gathered. Key words: active nature protection, faunistic research, NIP project th
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O‐2 ASSESSING PROTECTED AREA VALUES AND BENEFITS IN THE DINARIC ARC A. Štefan1, S. Stolton2, D. Porej1, K.Z. Ivanić1 1 WWF Adria, Zagreb, Croatia ([email protected]) 2 Equilibrium Research, Bristol, UK WWF recently completed a protected areas benefit assessment in 58 parks, representing over 50% of all protected territory in the Dinaric Arc. The Protected Area Benefits Assessment Tool (PA‐BAT) assesses the full range of legal benefits arising from protected areas, using a structured questionnaire in a participatory way. The PA‐BAT looks at issues ranging from ecosystem services such as clean water, local products, sustainable fisheries, to tourism and less quantifiable values. In many of the national and nature parks the workshop has been the first opportunity for local communities, water and energy companies, tourism experts and fisher folk to meet park staff and discuss what the protected area means to them. In many cases, managers and other workshop participants have been unaware of the benefits that are important to other stakeholders. The PA‐BAT has been appli ed in about 60 PAs in eight countries, from Slovenia to Albania; certainly the largest assessment of protected area benefits in the world to date. The results will inform protected area management plans, rural development, interpretation and education programmes to help influence day to day management decisions. We will present an update of the PA‐BAT methodology and consolidated results, discuss lessons learned about its use at this scale to help other users; outline how the PA‐BAT results can feed into PA management and policy; and look at strategic implications for the Dinaric Arc. Key words: Protected Area Benefits Assessment Tool (PA‐BAT), Dinaric Arc Parks, WWF O‐3 PRVA PRIMJENA RADARSKE ORNITOLOGIJE NA PODRUČJU BALKANSKE REGIJE E. Patčev1, M. Grgurev2, A. Avdaković3, M. Maslać4, A. Bukovac1, H. Peternel1 1 Geonatura d.o.o. za stručne poslove zaštite prirode, Zagreb, Hrvatska ([email protected]) 2 Gekom d.o.o. geofizikalno i ekološko modeliranje, Zagreb, Hrvatska 3 JP Elektroprivreda BiH, Sarajevo, Bosna i Hercegovina 4 Oikon d.o.o. – Institut za primijenjenu ekologiju, Zagreb, Hrvatska Na temelju podataka prikupljenih uz pomoć radarskog sustava i terenskih ornitoloških istraživanja, moguće je donijeti preciznu procjenu o karakteristikama migracije, posebno o smjeru, visini i intenzitetu. Istraživanje je provedeno u Bosni i Hercegovini, na planini Velež. Osnovni cilj istraživanja je prikupljanje podataka o aktivnosti i kretanju ptica i otkrivanje mogućeg migracijskog koridora na istraživanom području uz pomoć radarskog sustava. U istraživanju su istovremeno korištena 2 morska radara u X frekvencijskoj zoni koji su radili u različitim modovima (vodoravni i uspravni). Podaci su sakupljani u periodu 24.8.‐26.9.2014. Radarski podaci (signali na radarskom zaslonu) snimljeni su uz pomoć 'frame grabber' kartice na računalo. Sakupljeni podaci sastojali su se od nekoliko mjera koje uključuju udaljenost signala i horizontalnu udaljenost od radarske antene, kao i visinu signala. Uz pomoć softvera Distance izračunat je korekcijski faktor kako bi se u izračun uključila različita vjerojatnost detekcije signala s obzirom na njegovu udaljenost od antene radara. Rezultati pokazuju razliku u dnevnom i sezonskom intenzitetu migracije. Upotreba radarskog sustava pridonosi boljem th
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poznavanju migracije s obzirom na vertikalnu distribuciju i smjer migracije. Kako bi se dobili kompletni podaci o migraciji na određenom području, potrebno je kombinirati radarski sustav i standardne ornitološke metode, a idealno bi bilo potkrijepiti ih s lokalnim i regionalnim meteorološkim podacima. Ključne riječi: radar, migracija ptica, radarska ornitologija, vertikalna distribucija leta, softver Distance THE FIRST APPLICATION OF THE RADAR ORNITHOLOGY IN THE BALKAN REGION E. Patčev1, M. Grgurev2, A. Avdaković3, M. Maslać4, A. Bukovac1, H. Peternel1 1 Geonatura Ltd., Zagreb, Croatia ([email protected]) 2 Gekom Ltd. – Geophysical and Ecological Modelling, Zagreb, Croatia 3 JP Elektroprivreda BiH, Sarajevo, Bosnia and Herzegovina 4 Oikon Ltd. – Institute of Applied Ecology, Zagreb, Croatia Based on data collected from both radar system and a field ornithologist’s surveys, a precise approximation of the bird migration patterns can be made, especially on data such as birds’ flight direction, altitude and intensity. The survey was conducted in Bosna and Herzegovina, on Velež mountain. The main goal was to collect bird activity and movements’ data with radar system in order to investigate possible migration corridor over the area.For collection of migration data two X‐band marine radars were used simultaneously, working in two different modes (horizontal and vertical). Data was collected in the period from 24th August to 26th September 2014. The radar data (signals on the radar screen) were captured using a 'frame grabber' card and recorded to a computer. Collected data consisted of several distance measurements in relation to radar antennas including Euclidean distance, altitude and land distance. To count a distance‐dependant probability of radar signals, a correction factor was calculated using Distance software. Results showed seasonal and diurnal difference of migration intensity. Use of radar system greatly contributes to better understanding of migration patterns especially vertical distribution of migration and migration direction. In order to acquire complete data on bird migration, it is necessary to integrate the radar system with standard ornithological research methods. Ideally, this would be substantiated with local and regional meteorological data. Key words: radar, bird migration, radar ornithology, migration pattern, flight attitude, Distance software O‐4 MEĐUNARODNO VAŽNA PODZEMNA SKLONIŠTA ZA ŠIŠMIŠE HRVATSKOJ ‐ PRIMJENA U OČUVANJU I ZAŠTITI D. Hamidović, S. Desnica Državni zavod za zaštitu prirode, Zagreb, Hrvatska ([email protected]) U Hrvatskoj je zabilježeno 55 međunarodno važnih skloništa za šišmiše predano UNEP/EUROBATS Sporazumu. U podzemna skloništa uvrštene su špilje, jame, rudnici, željeznički tuneli i tuneli za odvodnju. Svako sklonište sadrži sljedeće podatke: koordinate, ime objekta, tip objekta, primarno korištenje (porodiljska kolonija, hibernacija, migracije, cjelogodišnje korištenje), ime i kod POVS područja, biogeografsku regiju u kojoj se nalazi, broj th
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vrsta šišmiša koji je u skloništu zabilježen i zaštita skloništa (težak pristup, zatvoreno vratima, otvoreno za turističke potrebe). Za svaku vrstu u skloništu naveden je godina u kojoj je zabilježen najveći broj jedinki od 1994. godine te način korištenja skloništa. Ukupno je u ovim podzemnim skloništima zabilježeno 25 vrsta šišmiša od 34 prisutne u Hrvatskoj. U pravilu najveće hibernacijske kolonije nalaze se u kontinentalnoj biogeografskoj regiji, dok su u mediteranskoj najveće porodiljne kolonije većine vrsta. U ekološkoj mreži Natura 2000 nalaze se svih 55 skloništa te je za sve zahvate, planove i programe potrebno sagledati utjecaj na ciljne vrste šišmiša. Tada se provode predviđeni zakonski mehanizmi koji na razini strateškog planiranja, prostornog plana ili samog zahvata uzimaju u obzir tip skloništa, način korištenja skloništa te ekologije ciljne vrste šišmiša. Predstavljamo kriterije i primjere ocjene utjecaja na razini strateške ocjene županijskog prostornog plana te nekoliko ocjena zahvata vjetroelektrana. Ključne riječi: šišmiši, podzemna skloništa, zakonodavstvo, očuvanje, glavna ocjena utjecaja INTERNATIONALLY IMPORTANT UNDERGROUND SITES FOR BATS ‐ CONSERVATION AND PROTECTION APPLICATION D. Hamidović, S. Desnica State Institute for Nature Protection, Zagreb, Croatia ([email protected]) There are 55 Internationally important underground roosts for bats submitted to UNEP/EUROBATS Agreement. Underground sites in Croatia refer to caves, pits, mines, railway tunnels and waterway tunnels. Each site has following data: coordinates, name, type, primary usage (all year, hibernation maternity, transient/migratory), name and code of pSCI, biogeographical region, number of bat species recorded and protection (gates, grilles, difficult access, open for public). For each bat species per site the year in which the biggest number of individuals recorded since 1994 is given together with the type of usage. In all underground sites listed, altogether 25 bat species out of 34 present in Croatia are recorded. As a general rule for the most species the most numerous hibernacula are in the continental biogeographical region, whereas in the mediterranean biogeographical region the biggest maternities are present. All 55 underground sites are a part of the Natura 2000 Ecological Network which means that for every project, plan and programme an impact to the Natura site conservation objectives should be assessed. Legal mechanisms required at the strategic planning and physical planning level or project level are presented taking into account roost type, usage type and ecology of bat species. Provided are examples of criteria and evaluation of strategic impact assessment of the county physical plan and Appropriate assessment on the project level of several windfarms. Key words: bats, underground roosts, legislation, conservation, appropriate assessment O‐5 SUSTAV ZA MULTISPEKTRALNU ANALIZU VEGETACIJE ZA BIOLOŠKA I EKOLOŠKA ISTRAŽIVANJA D. K. Hackenberger, T. Vondrak Odjel za biologiju, Sveučilište Josipa Jurja Strossmayera, Osijek ([email protected]) Istražena je mogućnost uporabe autonomnog letećeg uređaja (ALU) za proučavanje vegetacije iz zraka za potrebe bioloških i ekoloških istraživanja. Prilikom istraživanja razvijen je jednostavni i mobilni sustav za daljinsko očitavanje vegetacijskih indeksa (NDVI, EVI, SAVI/MSAVI). Ovakav th
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sustav omogućava efektivno i brzo prikupljanje podataka o vegetaciji iz zraka. Sama tehnika detektiranja vegetacijskog indeksa temelji se na dvjema jednakim komercijalnim HD kamerama. Jedna od kamera je bila modificirana za detekciju valnih duljina u blisko infracrvenom području (near‐infrared, NIR) dok je druga snimala valne duljine vidljive svjetlosti. Slike dobivene kamerama su se pohranjivale i obrađivale tijekom leta čime je omogućeno neposredno korištenje rezultata u daljnjim znanstvenim istraživanjima. Preliminarna analiza dobivenih podataka pokazuje veliki potencijal za korištenje ovakvog sustava u biološkim i ekološkim istraživanjima. Ključne riječi: vegetacijski indeksi, NIR, ALU, kamera, daljinsko očitavanje MULTISPECTRAL VEGETATION ANALYSIS SYSTEM FOR BIOLOGICAL AND ECOLOGICAL RESEARCH D. K. Hackenberger, T. Vondrak Department of biology, Josip Juraj Strossmayer University, Osijek ([email protected]) Possibility of usage of unmanned aerial vehicle (UAV) for airborne vegetation observation for demand of biological and ecological studies has been researched. Throughout the study a simple and mobile system for remote sensing of vegetation indices (NDVI, EVI, SAVI /MSAVI) has been developed. This kind of system facilitates effective and rapid accumulation of aerial vegetation data. The aforementioned technique is based on two identical consumer‐grade HD cameras. One of these cameras has been modified to detect near‐infrared wavelengths (NIR), while the additional camera recorded visible spectrum wavelengths. Images given by the cameras were being stored and processed during the flight, this enabled point‐blank usage in further scientific research. Preliminary analysis of given data shows promising potential for use of this kind of system in biological and ecological research. Key words: Vegetation indices, NIR, UAV, Camera, Remote sensing O‐6 ENDEMIC AND RELICTS SPECIES IN SPECIAL NATURE RESERVE ZASAVICA M. Stanković Pokret gorana Sremska Mitrovica‐SRP Zasavica, Sremska Mitrovica, Serbia ([email protected]) The tertiary relics of the flora of the reserve are: Stratiotes aloides, Trapa natans agg., Butomus umbellatus, Erithronium dens‐canis, Humulus lupulus, Hidrocharis morsus‐ranae, Isopirum thalictroides, Loranthus europaeus, Tamus communis, Viscum album and Aldrovanda vesiculosa. The Special Nature Reserve 'Zasavica' is the only habitat in Serbia for the plant Aldrovanda vesiculosa, which was long thought to be extinct in Serbia. The three species of flora of the reserve are glacial relicts Urtica kioviensis, Scirpus triqueter, Thelypteris palustris, and Achillea asplenifolia is the Pannonian subendemic. The fauna of the reserve has recorded four endemic and relicts species of invertebrates and vertebrates. From invertebrates there is found a rare endemic branchiopods shrimp Chirocephalus brevipalpis and a relic living fossil Lepidurus apus. The entomofauna of the reserve has two endemic species Stenopterus similatus and Zeuneriana amplipennis which is found again after more than thirty years. From the class of Gastropoda there was identified a Balkan endemic species of Tandonia kusceri. A th
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special rarity of the reserve is the globally endangered relict species Umbra krameri for which the flow Zasavica is one of the three remaining habitats in Serbia, and this subpopulation is one of the most endangered in the whole complex in Europe. The fauna of amphibians and reptiles present three Balkan endemics Triturus dobrogicus, Lacerta agilis ssp.bosnica and Vipera berus bosniensis (black melanistic form in the reserve) which in Posavina has a relict character.
O‐7 LIŠAJEVI KAO BIOINDIKATORI KVALITETE ZRAKA NA PODRUČJU MOTOVUNSKE ŠUME
M. Maslać1, A. Partl2, Z. Mesić3, O. Antonić4,5 1 Geonatura d.o.o. za stručne poslove zaštite prirode, Zagreb, Hrvatska ([email protected]) 2Državni zavod za zaštitu prirode, Zagreb, Hrvatska 3 Oikon d.o.o. Institut za primjenjenu ekologiju, Zagreb, Hrvatska 4 Oikon d.o.o. Institut za primijenjenu ekologiju/Geonatura d.o.o. za stručne poslove zaštite prirode/Gekom ‐ Geofizikalno i ekološko modeliranje d.o.o., Zagreb, Hrvatska 5 Odjel za biologiju, Sveučilište J. J. Strossmayera u Osijeku, Osijek, Hrvatska
Lišajevi su među najpoznatijim biološkim indikatorima za procjenu kvalitete zraka jer svu vodu potrebnu za život u cijelosti primaju iz zraka. Obzirom na navedeno, mogu se koristiti za procjenu stanja okoliša uzimajući u obzir više izvora zagađenja i različite efekte (eng. multi‐ pollutant/multi‐effect). Određivanjem vrsta i brojnosti lišajeva, moguće je mapirati učinke dugoročnog (kumulativnog) onečišćenja zraka na velikim područjima. Korištenjem lišajeva kao bioindikatora određena je kvaliteta zraka na području Motovunske šume u sklopu projekta 'Plan restauracije starog korita Mirne ‐ Biološko‐ekološka istraživanja i podloge'. Na 18 ploha je izvršeno uzorkovanje lišajeva te su određene prisutne vrste i njihova učestalost. Prema prisutnim vrstama i njihovoj abundaciji, zaključeno je kako se gotovo cijelo područje odlikuje iznimno visokom kvalitetom zraka kao i visokom količinom vlage u zraku. Istraživanjem je utvrđeno kako u Motovunskoj šumi, u kojoj su debla većinom prekrivena mahovinom, nije moguće odrediti kvalitetu zraka prema standardiziranoj metodi za određivanje kvalitete zraka pomoću lišajeva u Europi. Stoga se za vlažne šume slične Motovunskoj šumi predlaže korištenje metode koja se trenutno primjenjuje u SAD‐u, a na koju ne utječe količina mahovina. Rezultati potvrđuju primijenjivost novih ekoloških indeksa za slična istraživanja kvalitete zraka i općeg stanja istraživanog područja. Ključne riječi: lišajevi, onečišćenje zraka, kvaliteta zraka, bioindikatori, vlažne šume LICHENS AS BIOINDICATORS OF AIR QUALITY IN THE MOTOVUN FOREST AREA M. Maslać1, A. Partl2, Z. Mesić3, O. Antonić4,5 1 Geonatura Ltd., Zagreb, Croatia ([email protected]) 2 State Institute For Nature Protection, Zagreb, Croatia 3 Oikon Ltd. Institute of Applied Ecology, Zagreb, Croatia 4 Oikon Ltd. Institute of Applied Ecology /Geonatura Ltd. /Gekom Ltd.Geophysical and ecological modelling, Zagreb, Croatia 5 Biology Department, J. J. Strossmayera University of Osijek, Osijek, Croatia Lichens are among the best known biological indicators for assessing the air quality, due to their ability to fully receive all water necessary for life directly from the air. Hence, they are important because they can be used to estimate the multi‐pollutant/multi‐effect environment condition. By determining the species and the number of lichens, it is possible th
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to map the effects of long‐term (cumulative) air pollution in large areas. By using lichens as bioindicators the air quality in the Motovun forest area was determined as part of the 'Plan for the restoration of the Mirna river old bed ‐ Biological and ecological research and background'. On the 18 plots lichens were sampled and present species and their frequency was determined. It was concluded that almost the entire area is characterized by extremely high air quality, as well as a high amount of air moisture. The survey identified shortcomings in the existing standardized method for determining air quality using lichens in Europe, when working in a humid forest with high moss abundancy on the tree trunk. When working in the woods with humidity levels comparable to this area, it is proposed to use the method currently used in the USA that is not biased with the moss on the tree trunk. The results confirm the applicability of new environmental indices for similar studies of air quality and general condition of the study area. Key words: lichens, air pollution, air quality, bioindicators, humid forest O‐8 UTJECAJ METEOROLOŠKIH PARAMETARA NA VARIJACIJE DVOSATNIH KONCENTRACIJA PELUDNIH ZRNACA ČEMPRESA (CUPRESSACEAE), GRAD ZADAR, HRVATSKA A. Vucić1, D. Peroš‐ Pucar1, B. Mitić2, I. Hrga3, B. Pucar1 1 Služba za zdravstvenu ekologiju, Zavod za javno zdravstvo Zadar, Zadar, Hrvatska ([email protected]‐ zadar.hr) 2 Biološki odsjek, Priridoslovno‐ matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 3 Služba za zaštitu okoliša i zdravstvenu ekologiju, Nastavni zavod za javno zdravstvo 'Dr. Andrija Štampar', Zagreb, Hrvatska Pelud iz porodice čempresa (Cupressaceae) najbrojnija je pelud u atmosferi grada Zadra s udjelom od gotovo 50% u ukupnom broju peludnih zrnaca u zraku. Smatra se da peludna zrnca iz porodice čempresa imaju umjeren do visok alergeni potencijal te oni spadaju u glavne alergene na mediteranskom području. Svrha istraživanja je utvrditi dvosatne fluktuacije peludnih zrnaca iz porodice čempresa u danima sa zabilježenom visokom koncentracijom (> 90 pz/m3) te utvrditi utjecaj nekih meteoroloških parametara na dvosatne varijacije peludnih zrnaca čempresa. Istraživanje je provedeno tijekom 2007.‐ 2009. na temelju preporuka EAN / REA. Aerobiološki uzorci su prikupljeni pomoću automatskog sedmodnevnog volumetrijskog uzorkivača tipa Hirst. Dobiveni rezultati pokazuju da je pelud iz porodice čempresa (Cupressaceae) prisutna u atmosferi grada Zadra tijekom cijele godine, ali većina peludnih zrnaca čempresa zabilježena su tijekom zimskih mjeseci (od veljače do travnja). Na varijacije dvosatnih koncentracija peludnih zrnaca čempresa statistički značajan i pozitivan utjecaj imaju temperatura, brzina vjetra i broj sunčanih sati, dok je utjecaj relativne vlage zraka statistički značajan i negativan. Ključne riječi: aerobiologija, Cupressaceae, dvosatne koncentracije, Hirst, Zadar METEOROLOGICAL INFLUENCE ON DIURNAL CUPRESSACEAE POLLEN VARIATION (ZADAR, CROATIA) A. Vucić1, D. Peroš‐ Pucar1, B. Mitić2, I. Hrga3, B. Pucar1 1 Department of Health Ecology, Institute of Public Health Zadar, Zadar, Croatia ([email protected]‐zadar.hr) 2 Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia 3 Department of Enviromental Protection and Health Ecology , Institute of Public Health 'Dr. Andrija Štampar', Zagreb, Croatia th
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Cupressaceae pollen is the most abundant pollen in the atmosphere of Zadar with a share of almost 50% in total pollen count. The allergenic potential of Cupressaceae pollen is moderate to high and it represents the major airborne allergen in Medierranean area. The purpose of this study was to establish two‐hour interval daily fluctuations of Cupressaceae pollen during days with marked high concentration (>90 pg/m3) and to determine influence of some meterological parameters on diurnal Cupressaceae pollen variation. The study was carried during the 2007‐ 2009 using the Spanish Aerobiological Network (EAN/ REA) recommendation. Aerobiological samples were collected via Hirst‐type, seven‐ day volumetric sampler. The obtained results showed that the Cupressaceae pollen is present in the Zadar atmosphere during the whole year, but the majority of Cupressaceae pollen is marked during the winter months (from February till April). Correlations between Cupressaceae pollen diurnal concentration and meteorological parameters were statistically significant: influence of temperature, wind speed and hours of sunshine were positive and influence of relative humidity were negative. Key words: aerobiology, Cupressaceae, diurnal concentration, Hirst, Zadar
O‐9 MLADICA (Hucho hucho) NA BALKANU: RASPROSTRANJENOST I BUDUĆI UTJECAJI HIDROENERGETSKIH PLANOVA
J. Freyhof1, S. Weiss2, A. Adrović3, M. Ćaleta4, A. Duplić5, B. Hrašovec6, B. Kalamujić7, Z. Marčić8, D. Milošević9, M. Mrakovčić8, D. Mrdak9, M. Piria10, P. Simonović11, S. Šljuka12, T. Tomljanović10, D. Zabric13 1 German Centre for Integrative Biodiversity Research, Leipzig, Njemačka 2 Institute of Zoology, Karl‐Franzens University Graz, Graz, Austrija 3 Prirodno‐matematički fakultet, Univerzitet u Tuzli, Tuzla, Bosna i Hercegovina 4 Učiteljski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 5 Državni zavod za zaštitu prirode, Zagreb, Zagreb, Hrvatska 6 Šumarski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 7 Institut za genetičko inženjerstvo i biotehnologiju, Sarajevo, Bosna i Hercegovina 8 Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) 9 Prirodno‐matematički fakultet, Univerzitet Crne Gore, Podgorica, Crna Gora 10 Agronomski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 11 Biološki fakultet, Univerzitet u Beogradu, Beograd, Srbija 12 Prirodno‐matematički fakultet, Univerzitet u Sarajevu, Sarajevo, Bosna i Hercegovina 13 Zavod za ribištvo Slovenije, Ljubljana, Slovenija
Mladica je jedna od najzagonetnijih slatkovodnih riba Europe. Povijesno, ova je vrsta bila široko rasprostranjena čitavim dunavskim slijevom. Od druge polovine 19. stoljeća, ukupna se brojnost mladice smanjila za dvije trećine pa su preostale populacije jako ugrožene, posebice razvojem hidroelektrana. U ovom je radu pregledano stvarno područje rasprostranjenosti mladice na Balkanu. Utvrđena su 1842 km rijeka u kojima postoji samoodrživa populacija mladice, čime je ovo područje postalo žarište za ovu vrstu. Ove se populacije nalaze u 43 različite rijeke ili odvojena riječna područja u Sloveniji, Hrvatskoj, Bosni i Hercegovini, Srbiji i Crnoj Gori. Oko 65% svih rijeka koje nastanjuje mladica nalaze se u ovim državama, što naglašava važnost balkanskih rijeka za preživljavanje ove vrste. Najveća je prijetnja ovim populacijama plan gradnje hidroelektrana. Ukupno su identificirana 93 projekta za brane izravno na rijekama u kojima živi mladica i veliki broj dodatnih projekata na pritocima ili th
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vodama uzvodno od sadašnjeg staništa mladice, koji će nedvojbeno degradirati stanišne uvjete nizvodno. Ako se ovi planovi izvedu, predviđamo da će najmanje 60‐70% balkanskih populacija i 35‐40% svjetske populacije mladice biti izgubljeno. Nadalje, preostale će populacije biti male i fragmentirane, što će im onemogućiti dugoročno preživljavanje.
Ključne riječi: mladica, Balkan, hidroenergija
THE HUCHEN (Hucho hucho) IN THE BALKAN REGION: DISTRIBUTION AND FUTURE IMPACTS BY HYDROPOWER DEVELOPMENT J. Freyhof1, S. Weiss2, A. Adrović3, M. Ćaleta4, A. Duplić5, B. Hrašovec6, B. Kalamujić7, Z. Marčić8, D. Milošević9, M. Mrakovčić8, D. Mrdak9, M. Piria10, P. Simonović11, S. Šljuka12, T. Tomljanović10, D. Zabric13 1 German Centre for Integrative Biodiversity Research, Leipzig, Germany 2 Institute of Zoology, Karl‐Franzens University Graz, Graz, Austria 3 Faculty of Sciences, University of Tuzla, Tuzla, Bosnia and Herzegovina 4 Faculty of Teacher Education, University of Zagreb, Zagreb, Croatia 5 State Institute for Nature Protection, Zagreb, Zagreb, Croatia 6 Faculty of Forestry, University of Zagreb, Zagreb, Croatia 7 Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina 8 Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) 9 Faculty of Sciences and Mathematics, University of Montenegro, Podgorica, Montenegro 10 Faculty of Agriculture, University of Zagreb, Zagreb, Croatia 11 University of Belgrade, Faculty of Biology, Belgrade, Serbia 12 Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina 13 Fisheries Research Institute of Slovenia, Ljubljana, Slovenia The Huchen is one of the most enigmatic species of Europe's freshwater fauna. Historically, the species was wide‐spread across the entire Danube basin. Since the late 19th century, however, Huchen populations declined by two thirds and the remaining populations are now highly endangered by hydropower development. In this study, we review the actual occurrence of Huchen in the Balkan region. A total of 1842 river km supporting self‐sustaining populations of Huchen in the region have been identified, making it the global hot spot for the species. These populations are found in 43 rivers or distinct river reaches in Slovenia, Croatia, BIH, Serbia and Montenegro. About 65% of all Huchen rivers globally are located in these countries, highlighting the importance of Balkan Rivers for the survival of the species. The major threat to these populations is a massive hydropower development plan. A total of 93 dam projects were identified directly in river reaches supporting Huchen and a large number of additional projects are located in tributaries or headwater reaches upstream of Huchen habitat that will invariably degrade environmental conditions downstream. If these plans are carried out, we predict that at least 60‐70% of the Balkan population and about 35‐40% of the global population of Huchen would be lost with the remaining populations being small and severely fragmented and eventually no longer able to survive in the long term. Key words: Huchen, Balkan, hydropower O‐10 PROCJENA USLUGA EKOSUSTAVA NA PODRUČJU DRAVA‐MOLVE PREMA TRI POTENCIJALNA SCENARIJA D. Pithart1, A. Duplić2, P. Kutleša2, I. Petrov Rančić2, N. Trenc2 th
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1
Beleco, Prag, Češka Odjel za ocjenu prihvatljivosti zahvata za prirodu, Državni zavod za zaštitu prirode, Zagreb, Hrvatska ([email protected]) Poplavne nizine Save, Drave i Dunava u Hrvatskoj pod utjecajem su različitih ljudskih aktivnosti, uključujući izgradnju naselja, poljoprivredu, promet i proizvodnju energije. One također imaju velik značaj u pružanju pitke vode, zaštiti od poplava, kao staništa brojnih vrsta, u produkciji drvne mase, ribe i divljači. Stoga informacije o funkcijama i uslugama ekosustava tog područja valja uzeti u obzir prilikom izrada prostornih planova, različitih strategija te donošenja odluka u javnom i privatnom sektoru. Istraživanje je obuhvatilo 38 km riječnog toka Drave, kod općine Molve, s poplavnom nizinom ukupne površine 201 km2. Cilj je bio dodatno argumentirati potrebu za očuvanjem riječnih ekosustava kroz promoviranje prirode kao osnove za razvoj. Analizirane su različite opskrbne, regulacijske, podržavajuće i kulturološke funkcije. Njihove vrijednosti procijenjene su prema tri potencijalna scenarija korištenja ovog područja u budućnosti: A održavanje postojećeg stanja, B provedba planiranih projekata (izgradnja HE Molve 1 i 2) i C razvoj kroz održivo korištenje prostora što uključuje proširenje poplavne zone. Korištene metode ovisile su o vrsti usluge i o ulaznim podacima. Temelj za analizu u scenariju B bili su utjecaji i promjene na uslugama ekosustava uzrokovane postojećim HE na Dravi u Hrvatskoj. Scenarij C pokazuje najvišu vrijednost za tri ključne usluge ekosustava: proizvodnji drva, osiguravanju staništa i ublažavanju poplava, dok je utjecaj scenarija B pretežno negativan. Ključne riječi: Drava, usluge ekosustava, procjena, HE Molve ASSESSMENT OF ECOSYSTEM SERVICES FOR THE AREA DRAVA‐MOLVE ACCORDING THREE POTENTIAL SCENARIOS D. Pithart1, A. Duplić2, P. Kutleša2, I. Petrov Rančić2, N. Trenc2 1 Beleco, Praha, Czech Republic 2 Department for nature impact assessment, State Institute for Nature Protection, Zagreb, Croatia ([email protected]) Floodplains of Sava, Drava and Danube Rivers in Croatia are a target of a variety of human activities, such as settlements, agriculture and transport or energy production. Also, they have potential to provide drinking water, flood protection, habitats for numerous species, timber production as well as fish and game animals. Information about ecosystem functions and services of this area should be taken into consideration during preparation of physical plans, strategies and by public and private sector decision making. In research is included 38 km of the Drava River, in municipality Molve and its floodplain, altogether 201 km2. The main goal was to get additional argumentations for purpose of riverine ecosystem conservation through promotion of nature as a basis for development. Regulating, supporting, provisioning and cultural functions were analyzed. Their values w ere evaluated according three possible future scenarios: A the status as it is, B the business as usual (with HP Molve 1 and 2) and C sustainable development characterized by extending of active floodplain zone. Used methods depend on type of services and data integrity. For the scenario B were analyzed impacts and changes on ecosystem services as consequences of the three existing HP on the Drava River in Croatia. The scenario C provides the highest value for the three key services: wood production, habitats provision and flood mitigation. On the other hand the impact of the scenario B is mostly negative. Key words: Drava, ecosystem services, assessment, HP Molve 2
th
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O‐11 MODELIRANJE POPULACIJE VRSTE SLABO ISTRAŽENIH KARAKTERISTIKA – PRIMJER POPOVSKE GAOVICE (Delminichthys ghetaldii) NA PODRUČJU OMBLE B. K. Hackenberger, O. Antonić, Ž. Lončarić Zavod za kvantitativnu ekologiju, Odjel za biologiju, Sveučilište Josipa Jurja Strossmayera u Osijeku ([email protected]) Matematički modeli su sastavni dio svakog suvremenog rješavanja problema u okolišu jer osim pronalaženja matematičkih zakonitosti problema modeli mogu u većoj ili manjoj mjeri sadržavati predikcijsku snagu iskoristivu za donošenje odluka te planiranje i monitoring mjera zaštite prirode i okoliša. Popovska gaovica (Delminichthys ghetaldii) je ugrožena vrsta šaranki koja obitava na širem području hercegovačkog i južnodalmatinskog krša. Za potrebe rješavanja jednog od okolišnih problema zahvata HE Ombla, bilo je potrebno ustanoviti da li primjerci ove vrste, koji obitavaju na području izvorišta rijeke Omble, čine samostalnu populaciju ili su samo dio veće populacijske cjeline (metapopulacije). Posebna teškoća u rješavanju ovog problema činila je činjenica da je popovska gaovica relativno slabo istražena i da je vrlo malo dostupnih podataka. Na temelju postojećih podataka, osobnih iskustava istraživača i njihovog mišljenja te teorije, načinjeno je nekoliko hipoteza. Primjenom diskretnog matričnog modeliranja u sprezi s metodama opetovanog podatkovnog uzorkovanja, utvrdile su se vjerojatnosti svake od pretpostavljenih hipoteza. Pokazano je da je popovska gaovica prisutna na području izvorišta rijeke Omble dio puno veće populacije te da bi zahvat HE Ombla, barem što se tiče opstanka ove vrste na tom području, imao pozitivan učinak. Ključne riječi: dinamika populacija, popovska gaovica, Ombla MODELING POPULATION OF A POORLY INVESTIGATED SPECIES ‐ EXAMPLE ON Delminichthys ghetaldii IN THE RIVER OMBLA B. K. Hackenberger, O. Antonić, Ž. Lončarić Subdepartment of Quantitative Ecology, Department of Biology, Josip Juraj Strossmayer University ([email protected]) Mathematical models are part of every modern environmental problem solving process. Besides finding mathematical principles models can have a strong prediction power that can be used for planning and monitoring purposes and can be useful for policy makers. Delminichthys ghetaldii is endangered species of cyprinid family that can be found on wide Herzegovinian and the southern Dalmatian karst area. In order to solve one of the environmental problems of the project HPP Ombla, it was necessary to determine whether specimens of this species that inhabit the spring area of the Ombla, constitute an independent population or are only part of a larger population (metapopulation). Special difficulties in solving this problem accounted for the fact that the Delminichthys ghetaldii is relatively poorly investigated species and that there was very little information available. Based on existing data, personal experiences of researchers and their opinions and theories, we made several hypotheses. By using discrete matrix modeling in conjunction with the methods of resampling, we determined the probability of each of the proposed hypothesis. It has been shown that the Delminichthys ghetaldii population that is present in the Ombla spring area is part of the larger population and that a project HPP Ombla, at least concerning the survival of this species, could have a positive effect. th
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Key words: population dynamics, Delminichthys, Ombla O‐12 U POTRAZI ZA OČUVANIM RIJEKAMA: IDENTIFIKACIJA NAJZNAČAJNIJIH RIJEKA ZA ZAŠTITU PRIRODE U HRVATSKOJ K. Žganec1, F. Antonelli2, I. Popović Dujmović3, N. Jeran4 1 Sveučilište u Zadru, Odjel za nastavničke studije u Gospiću, Gospić, Hrvatska ([email protected]) 2 WWF Mediteranski Program, Rim, Italija 3 WWF Adria, Zagreb, Hrvatska 4 Ministarstvo zaštite okoliša i prirode, Zagreb, Hrvatska Ovaj rad je prvi pokušaj da se sveobuhvatno ocijeni ekološko stanje i konzervacijska važnost hrvatskih rijeka. Cilj predstavljene metodologije je određivanje najznačajnijih rijeka za očuvanje cjelokupne slatkovodne bioraznolikosti u Hrvatskoj. Upotrijebili smo kombinaciju ekoregionalnog pristupa s glavnim ciljem predstavljanja svih sastavnica bioraznolikosti pojedine ekoregije u kombinaciji s određivanjem ekološkog stanja rijeka. Nakon utvrđivanja tipologije određene su glavne jedinice analize: Ocjenjivani Riječni Segmenti (ORS). Ekološko stanje svakog ORS‐a određeno je kombinacijom pet kriterija: hidrologija, morfologija korita, kvaliteta vode, riparijska zona i korištenje zemljišta. Ekološko stanje svakog ORS‐a dobiveno je zbrajanjem ocjena svih kriterija (maks. vrijednost 50). Podaci o rasprostranjenosti ugroženih i endemskih riba i prisutnosti zaštićenih područja korišteni su za prioritizaciju ORS‐a u odličnom ili dobrom stanju. Ukupno smo analizirali 82 rijeke, ukupne dužine toka 4522 km u tri ekoregije: panonskoj, dinaridsko‐kontinentalnoj i dinaridsko‐mediteranskoj. Ukupna dužina toka odabranih rijeka od najveće važnosti iznosi 1607 km (22%), a dodatni ORS‐i, koji obuhvaćaju ukupno 998 km toka odabrani su kako bi se osigurala reprezentativnost svih riječnih tipova u svakoj ekoregiji. Odabrani ORS‐i predstavljaju najznačajnije riječne segmente, čija će zaštita osigurati dugoročno očuvanje najznačajnijih sastavnica slatkovodne bioraznolikosti u Hrvatskoj. Ključne riječi: slatkovodna bioraznolikost, ekoregionalni pristup, tipologija, ekološko stanje, rijeke LOOKING FOR PRISTINE RIVERS: IDENTIFICATION OF THE MOST OUTSTANDING RIVERS OF HIGH CONSERVATION VALUE IN CROATIA K. Žganec1, F. Antonelli2, I. Popović Dujmović3, N. Jeran4 1 University of Zadar, Department of Teachers' Education in Gospić, Gospić, Croatia ([email protected]) 2 WWF Mediterranean Programme Office, Rome, Italy 3 WWF Adria, Zagreb, Croatia 4 Ministry of Environmental and Nature Protection, Zagreb, Croatia Environmental status and conservation values of Croatian rivers have never been comprehensively assessed. We present a methodology for the identification of the most pristine rivers of high conservation value which are important for the protection of freshwater biodiversity in Croatia. The methodology is a combination of ecoregional conservation approach where representation of all distinct biodiversity features is a key goal, and assessment of river reach condition. Main units of analysis, the Evaluated River Reaches (ERRs), th
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were identified after all river types were distinguished. The condition of each ERR was assessed using a combination of five criteria: hydrology, channel morphology, riparian vegetation, water quality and land use. The final condition of ERR was scored (max. 50) by summing scores for the five criteria. The distribution of endangered freshwater f ish species and protected areas was used for prioritizing the ERRs in high and good condition. Altogether 82 rivers and 4522 km of river length were analyzed in three ecoregions: Pannonian, Dinaric‐Continental and Dinaric‐ Mediterranean. The total length of very high priority ERRs was 1607 km (35%), while additional ERRs comprising 998 km (22%) of river length were included to assure representation of all river types in each ecoregion. The selected ERRs represent priority river reaches whose protection should ensure the long term persistence of the most important features of freshwater biodiversity in Croatia. Key words: freshwater biodiversity, ecoregional conservation, typology, condition, rivers O‐13 PROTOKOL PROCJENE RIZIKA OD INVAZIVNOSTI STRANIH VRSTA KORNJAČA U HRVATSKOJ A. Duplić1, D. Jelić2, B. Barić1, B. Janev Hutinec3, P. Kutleša1, I. Cizelj4 1 Odjel za ocjenu prihvatljivosti zahvata za prirodu, Državni zavod za zaštitu prirode, Zagreb, Hrvatska ([email protected]) 2 Hrvatski institut za biološku raznolikost, Hrvatsko herpetološko društvo Hyla, Zagreb, Hrvatska 3 Javna ustanova Maksimir, Zagreb, Hrvatska 4 Ustanova Zoološki Vrt Grada Zagreba, Zagreb, Hrvatska Trgovina kućnim ljubimcima jedan je od putova unosa stranih vrsta u prirodu. Među najpoznatijim primjerima invazivne strane vrste koja je na taj način proširena svijetom je crvenouha kornjača (Trachemys scripta elegans). Nakon što je trgovina njome u Hrvatskoj zabranjena, došlo do značajnog smanjenja broja kornjača na tržištu te je nastala potreba za iznalaženjem drugih pogodnih vrsta. Da bi se dozvolilo stavljanje na tržište novih vrsta potrebno je isključiti postojanje ekološkog rizika u slučaju da vrsta dospije u prirodu. Budući da ne postoji standardna procedura za procjenu rizika od invazivnosti za kornjače, na temelju analiza kriterija iz literature i specifičnosti u Hrvatskoj, za tu je svrhu izrađen protokol. Prema protokolu, kriteriji za procjenu rizika mogu se podijeliti u četiri grupe koje obuhvaćaju podatke o rasprostranjenosti vrste u prirodi i područjima u kojima je vrsta kao strana uspostavila populaciju, o biološkim i ekološkim obilježjima vrste, podatke o povijesti invazivnosti te podatke o dostupnosti vrste na tržištu EU. Analiza rizika uključuje i procjenu klimatske pogodnosti staništa za što je izrađen model s kartografskim prikazom. Pitanja su zatvorenog tipa te su im pridružene vrijednosti od 0 do 3. Što je vrijednost niža to je potencijalni rizik manji. Od analiziranih vrsta one s najmanjim rizikom invazivnosti su Graptemys versa, Sternotherus carinatus i Ocadia sinensis dok su one s najvećim rizikom Apalone ferox, A. spinifera i Pelodiscus sinensis. Ključne riječi: kornjače, strane vrste, procjena rizika, invazivnost PROTOCOL FOR THE INVASIVENESS RISK OF THE ALIEN TURTLE SPECIES IN CROATIA A. Duplić1, D. Jelić2, B. Barić1, B. Janev Hutinec3, P. Kutleša1, I. Cizelj4 1 Department for nature impact assessment, State Institute for Nature Protection, Zagreb, Croatia ([email protected]) th
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Croatian Institute for Biodiversity, Croatian Herpetological Society Hyla, Zagreb, Croatia Public institution Maksimir, Zagreb, Croatia 4 Zoo Zagreb, Zagreb, Croatia The trade in pets is one of the main paths for introduction of alien species in nature. The most widely known example of the invasive species that has spread worldwide in this way is Trachemys scripta elegans. After the trade with this species was banned in Croatia the number of turtles on the market has significantly decreased and the need to find suitable species for the market has occurred. To permit the trade with a new species it is necessary to exclude the ecological risks in the case if it enters nature. As there is no standard procedure to assess invasiveness risk for turtles a protocol was created for this purpose, based on the criteria from literature and the specifics of Croatia. In accordance with the protocol criteria for risk assessment can be divided in four groups that include the data about distribution of the species in nature and in the areas in wh ich it has introduced and established a population, data about biological and ecological characteristics of the species, data about history of invasiveness and data about the availability of the species on the EU market. Risk analysis includes assessment of climate suitability. The questions are of the closed type with attributed values from 1‐3. The lower the attributed value the potential risk is lower. From analysed species, the species with the least invasiveness risk are Graptemys versa, Sternotherus carinatus i Ocadia sinensis and with the highest risk are Apalone ferox, A. spinifera and Pelodiscus sinensis. Key words: turtle, alien species, risk assessment, invasiveness O‐14 KAKO SE BORITI PROTIV INVAZIVNE VRSTE? BIOLOŠKO SUZBIJANJE KESTENOVE OSE ŠIŠKARICE UNESENOM VRSTOM PARAZITOIDA D. Matošević1, I. Franić2, I. Lukić1 1 Hrvatski šumarski institut, Jastrebarsko, Hrvatska ([email protected]) 2 Cuglini 1, Zagreb, Hrvatska Kestenova osa šiškarica (Dryocosmus kuriphilus) je invazivna vrsta kukca u cijelom svijetu i izuzetno brzo se širi u novom staništu. Smatra se jednim od najznačajnijih štetnika na pitomom kestenu (Castanea sativa). Autohtone vrste parazitoida koje su trofički vezane uz hrastove ose šiškarice (Cynipidae) su se prilagodile ovom novom invazivnom domaćinu, ali ne mogu učinkovito utjecati na smanjenje gustoće njegove populacije. Biološko suzbijanje koristeći unesenu vrstu parazitoida Torymus sinensis se pokazalo kao jedina učinkovita metoda kontrole populacije kestenove ose šiškarice, usporavajući njezino širenje i smanjujući štete na podnošljivu razinu. Visoka specifičnost parazitoida T. sinensis upućuje na ograničeni i nepostojeći potencijal iskorištavanja autohtonih domaćina. U Hrvatskoj se u 4 godine od pojave kestenove ose šiškarice na o vog novog domaćina prilagodilo 15 vrsta autohtonih parazitoida, ali oni nemaju nikakvog utjecaja na smanjenje gustoće njezine populacije. Zbog toga je u prirodne sastojine pitomog kestena u Hrvatskog ispušten uneseni parazitoid T. sinensis kao dokazano učinkovito sredstvo biološkog suzbijanja. U izlaganje se prikazuju rezultati uzgoja i ispuštanja parazitoida T. sinensis u Hrvatskoj. Ključne riječi: Dryocosmus kuriphilus, Torymus sinensis, autohtoni parazitoidi, invazivna vrsta kukca 3
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HOW TO CONTROL INVASIVE SPECIES? CLASSICAL BIOLOGICAL CONTROL OF Dryocosmus kuriphilus WITH INTRODUCED PARASITOID SPECIES D. Matošević1, I. Franić2, I. Lukić1 1 Croatian Forest Research Institute, Jastrebarsko, Croatia ([email protected]) 2 Cuglini 1, Zagreb, Croatia Dryocosmus kuriphilus is a globally invasive insect pest, spreading very quickly in new habitats and making seriously damage to sweet chestnut (Castanea sativa) forests in Croatia. Indigenous parasitoid species trophically associated with oak gallwasps (Cynipidae) have adapted to this new invasive host but cannot effectively regulate its population density. Classical biological control using introduced parasitoid Torymus sinensis has been proven to be the only effective method of controlling the populations of D. kuriphilus. T. sinensis can successfully control the population density of D. kuriphilus, slowing down the spread and mitigating negative impact of this invasive chestnut pest and keeping the damage of D. kuriphilus at acceptable level. High specificity of T. sinensis suggests that it has limited potential of exploiting native hosts. In Croatia, during 4 years 15 species of native parasitoids have adapted to new invasive host but have no impact on lowering the population density. T. sinensis has been released in sweet chestnut forests in Croatia as promising biological control agent. Results on laboratory rearing and release of T. sinensis in Croatia are presented. Key words: Dryocosmus kuriphilus, Torymus sinensis, native parasitoids, invasive insect species O‐15 ŠTETE I KORISTI INVAZIJA UNEŠENIH RIBA U SLIVU NERETVE B. Glamuzina Odjel za akvakulturu, Sveučilište u Dubrovniku, Dubrovnik, Hrvatska ([email protected]) Recentna istraživanja ukazuju da brojnost većine autohtonih vrsta opada kao rezultat aktivnosti čovjeka ('gubitnici'), a zamjenjuje ih značajno manji broj invazivnih vrsta u promijenjenim ekosustavima ('dobitnici'). Ovo je posebice vidljivo u slatkovodnim ekosustavima Mediterana s visokom razinom endemizma riba, obilježenoga uskim arealom većine vrsta. Jedno od važnih područja mediteranskoga endemizma riba je sliv Neretve s opisanih dvadeset stenoendemskh vrsta. Neretva je najdulja rijeka istočno‐jadranske obale (230 km), koja izvire u Bosni i Hercegovini, a utječe širokom močvarnom deltom u Hrvatskoj. Poslije 1960. godine rijeka je regulirana izgradnjom pet brana i formiranjem pet umjetnih akumulacija. Donji tok je melioriran, te je većina močvara i laguna pretvorena u poljoprivredno zemljište. Sve ove promjene dovele su do formiranja ekosustava novih značajka u odnosu na prirodne, te su se autohtone vrste riba našle pred izazovima prilagodba ili nestajanja. Dodatno su novonastali ekosustavi poribljavani nizom alohtonih vrsta, te je do danas zabilježeno dvadeset pet unešenih vrsta riba. Novija istraživanja su pokazala značajno povećanje brojnosti unešenih i smanjivanje brojnosti autohtonih vrsta riba. Ovo predavanje opisati današnju strukturu populacija riba u nekim ekosustavima, dati listu pobjednika i gubitnika, šteta i koristi u ekosustavima i ribarstvu i predloziti mjere za th
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ublažavanje najznačajnijih prijetnja. Ključne riječi: invazije riba, koristi i štete, sliv Neretve DAMAGES AND BENEFITS OF INTRODUCED FISH INVASIONS IN THE NERETVA RIVER WATERSHED B. Glamuzina Department for Aquaculture, University of Dubrovnik, Dubrovnik, Croatia ([email protected]) Recent evidences show that most species are declining as a result of human activities ('losers') and are replacing by a much smaller number of expanding species that thrive in man altered environments ('winners'). This is the case in freshwater ecosystems of the Mediterranean region, where a high level of endemism makes native fish extinctions much more likely because of the small natural ranges. One of these hotspots of Mediterranean endemism is the Neretva River watershed, with 20 stenoendemic species recently described there. The Neretva River is the longest river of the Eastern Adriatic watershed, 230 km long, originating in Bosnia and Herzegovina. Only the most downstream 20 km, which are wetlands and the estuary, are in Croatia. Since the 1960s the river and its flow is regulated due to the construction of five hydroelectric power dams leading to the formation of five reservoirs. The lower part was reclaimed and significant areas or wetlands and shallow lagoons were converted to agricultural land. This provided a completely new ecological situation, creating problems for the survival of the native fish fauna. The recent list of introduced fish in the Neretva River includes 25 species. Recent studies showed significant emerging of introduced fish populations and significant decrease of the native species. This presentation will discuss new fish community structure, present list of the winners and losers and describe measures to mitigate most significant threats. Key words: fish invasions, costs and benefits, Neretva watershed O‐16 PROTOKOL ZA DOJAVU I DJELOVANJE U SLUČAJU PRONALASKA UGINULIH, BOLESNIH ILI OZLIJEĐENIH STROGO ZAŠTIĆENIH MORSKIH ŽIVOTINJA K. Jelić, J. Jeremić Odjel za divlje i udomaćene svojte i staništa, Odsjek za more, Državni zavod za zaštitu prirode, Zagreb, Croatia ([email protected]) U Jadranskom moru obitava nekoliko vrsta kitova, dupina i morskih kornjača. Utjecaj ljudskih aktivnosti poput ribarstva i turizma čine te vrste osobito ranjivima. Uzimajući u obzir migracijsku prirodu ovih vrsta te zajedničku odgovornost država na Jadranu, suradnja je od velike važnosti za planiranje učinkovite i dugoročne strategije zaštite te je zbog navedenog pokrenut međunarodni projekt IPA Adriatic NETCET. Državni zavod za zaštitu prirode je u okviru razvoja nacionalnog Sustava za dojavu i djelovanje još 2010. godine započeo u suradnji s Državnom upravom za zaštitu i spašavanje (112) rad na uspostavi Protokola za dojavu i djelovanje u slučaju pronalaska uginulih, bolesnih ili ozlijeđenih strogo zaštićenih morskih životinja. U rad protokola do danas su uključeni obalne i otočne veterinarske ambulante, MOC Pula, Hrvatski veterinarski institut, Veterinarski fakultet u Zagrebu, te odjeli th
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i službe u županijama odgovorni za Eco brodice. Vrste za koje se najčešće primaju dojave su dobri dupin (Tursiops truncatus) i glavata želva (Caretta caretta). U proteklih pet godina zaprimljene su i dojave za plavobijelog dupina (Stenella coeruleoalba), krupnozubog dupina (Ziphius cavirostris) i kornjaču sedmoprugu usminjaču (Dermochelys coriacea). Svi podaci skupljeni putem postojećeg Protokola upisuju se u bazu i analiziraju, dio su monitoringa vrsta, daju uvid u prijetnje i pomažu u planiranju učinkovitije zaštite. Ključne riječi: Jadran, protokol, zaštićene morske vrste, kitovi, morske kornjače PROTOCOL FOR ALERTING AND MONITORING OF DEAD, SICK OR INJURED STRICTLY PROTECTED MARINE SPECIES K. Jelić, J. Jeremić Departement for Wild and Domesticated Species and Habitats, Sea Section, State Institute for Nature Protection, Zagreb, Croatia ([email protected]) Several species of cetaceanas, dolphins and sea turtles are recorded for the Adriatic Sea. Impact of human activities such as fishing and tourism makes those species highly vulnerable. Due to the migratory nature of those species cross‐border collaboration and shared management responsibility between Adriatic states is crucial in order to plan effective long‐term conservation strategies. For that purpose, an international IPA Adriatic project NETCET is launched. Since 2010, in the frame of National Alerting and Monitoring System, State Institute for Nature Protection started cooperation with National Protection and Rescue Directorate (112) on development of Protocol for Alerting and Monitoring of dead, sick or injured strictly protected marine species. At the moment, Protocol involves veterinary ambulances on the coast and islands, MEC Pula, Croatian Veterinary Insti tute, Faculty of Veterinary Medicine and County offices responsible for eco‐vessels. The most reported species are Bottlenose dolphin (Tursiops truncatus) and Loggerhead turtle (Caretta caretta). In the last five years there have also been reports on Striped dolphin (Stenella coeruleoalba), Cuvier's beaked whale (Ziphius cavirostris) and Leatherback turtles (Dermochelys coriacea). All data collected through the existing Protocol are registered in the database and analysed, they are the part of the monitoring of species, and they give an insight into threats and help in the planning of more effective protection. Key words: Adriatic, Protocol, protected marine species, cetaceans, sea turtles O‐17 KADA I DA LI LJUDI I ŠIŠMIŠI MOGU DIJELITI ISTO SKLONIŠTE? D. Hamidović1, V. Lindić2, P. Krstinić3 1 Državni zavod za zaštitu prirode, Zagreb, Hrvatska ([email protected]) 2 Ministarstvo zaštite okoliša i prirode, Uprava za inspekcijske poslove, Zagreb, Hrvatska 3 Javna ustanova za upravljanje zaštićenim dijelovima prirode Primorsko‐goranske županije 'Priroda', Rijeka, Hrvatska Ovaj rad naglašava važnost suradnje na civilnoj, stručnoj, zakonodavstvenoj i regionalnoj razini u očuvanju i zaštiti strogo zaštićenih vrsta šišmiša. Zbog gubitka skloništa primarno u šumskim tipovima staništa, neke vrste šišmiša prilagodile su se na suživot s ljudima. Rezolucija 5.7. UNEP/EUROBATS Sporazuma daje Smjernice za zaštitu šišmiša u nadzemnim th
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skloništima s posenim osvrtom na kulturnu baštinu. Manje vrste šišmiša roda Pipistrellus obitavaju u čitavom nizu skloništa vezanim uz ljudske nastambe uz uvjet da je otvor kroz koji mogu proći širi od 5 mm. Za razliku od njih vrste roda Rhinolophus biraju napuštene objekte ili tavanske prostore koji se ne koriste ili se koriste rijetko. Od 2009. godine provedeni su uviđaji na temelju dojave građana većim dijelom u središnjoj Hrvatskoj. Zabilježeni su tipovi, veličina i starost stambenih objekata, način korištenja i vrste šišmiša ukoliko je bilo moguće prema predviđenom Protokolu. Na temelju različitih tipova zahtjeva stanara u smislu obnove objekta ili problema zbog suživota predstavljena su moguća rješenja u vidu postavljanja kućica za šišmiše, potrebna zakonska procedura te uspješnost pozitivnih rješenja. Predstavljeni su i mehanizmi očuvanja i zaštite šišmiša u Starom gradu Bosiljevo kroz praćenje stanja i ograničavanje konzervatorskih radova na restauraciji dvorca. Ključne riječi: šišmiši, nadzemna skloništa, sinantropija, EUROBATS, mjere ublažavanja WHEN AND IF HUMANS AND BATS MAY SHARE THE SAME ROOST D. Hamidović1, V. Lindić2, P. Krstinić3 1 State Institute for Nature Protection, Zagreb, Croatia ([email protected]) 2 Ministry of Environmental and Nature Protection, Directorate for inspectional affairs, Zagreb, Croatia 3 Public Institution for Managing Protected Nature Areas ‐ County of Primorje & Gorski Kotar 'Priroda', Rijeka, Croatia In this work, importance of cooperation on citizen, expert, legislative and regional level in conservation and protection of strictly protected bat species is highlighted. Certain bat species adapted to the cohabitation with humans due to roost loss primarily in forests. Resolution 5.7 of UNEP/EUROBATS Agreement gives Guidelines for the Protection of Overground Roosts, with Particular Reference to Roosts in Buildings of Cultural Heritage. Smaller bat species of the genus Pipistrellus may find roosts that has opening more than 5 mm. Some other species of the genus Rhinolophus prefer abandoned houses or attics that are rarely used. Investigation based on the citizen calls were conducted since 2009 mostly in central part of Croatia. According to the proposed Protocol object type, age and usage together with bat species were recorded. Taking into account different demands of building owners like renovation or cohabitation conflict, different solutions in terms of bat box installation, the legislation procedure needed and success of positive solutions are presented. Conservation and protection mechanisms during restauration of Old Town Bosiljevo through monitoring and limitation of renovation works are given as an example. Key words: bats, overground roosts, sinantropy, EUROBATS, mitigation
2. Simpozij edukacije biologije 2nd Biology Education Symposium O‐18 SIMPLIFIED IDENTIFICATION KEYS FOR ORGANISMS B. Bajd Faculty of Education in Ljubljana, Ljubljana, Slovenia ([email protected]) th
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Simplified identification keys for organisms are important for many reasons. Using such keys and observing the details, we could learn how to find similarities and differences between organisms. We thus improve our powers of observation, which is nowadays quite often replaced by slipshod neglect. Furthermore, we can familiarise ourselves with the exceptional diversity of species and actually learn a few names. We also learn the basic structure of a key. Professional identification keys for plants and animals, used by biologists, are very complex and practically useless for laymen. They include numerous technical terms and too much information – they demand a great deal of prior knowledge from the reader. Using simplified identification keys, we can learn to classify plants and animals, which is one of the basic activities of natural sciences. And this encourages our thirst for knowledge, since we often want to find out more interesting facts about the various organisms, which we could look up later in other books or on the internet. If we find the right solution – the name of the animal or plant – we’re usually delighted and encouraged to research further. The knowledge obtained by observation with a simplified key thus becomes more permanent. However, we must keep in mind that this simple key will not help us identify all organisms but only a limited number of them. Using simplified key, we learn how keys are structured and how to properly read them, while we familiarise ourselves with characteristics of animals and plants and their adaptations to the environment in which they live. Key words: primary science education, biology education, identification keys for organisms, field work in nature O‐19 AFINITET UČENIKA ZA IZBOR ZANIMANJA U PODRUČJU BIOLOGIJE D. Garašić1, I. Radanović2, Ž. Lukša3, M. Sertić Perić2 1 Agencija za odgoj i obrazovanje, Zagreb, Hrvatska ([email protected]) 2 Biološki odsjek, PMF Sveučilišta u Zagrebu, Zagreb, Hrvatska 3 Gimnazija Josipa Slavenskog Čakovec, Čakovec, Hrvatska Istraživana je povezanost učeničkog opredeljenja za zanimanja u znanstvenom području biologije, s iskazanim interesima i životnim iskustvom. Istraživanje transverzalnog tipa provedeno je tijekom 2010. godine na uzorku 2114 učenika od 10 do 18 godina starosti. Afirmativni učenički odgovori za tematsko područje Razvoj i dosezi znanosti, sa srednjom vrijednošću ≥2,8 uzeti su kao pokazatelj istraživačke znatiželje. Utvrđeno je kako se učenici, s interesom za znanost u različitim dobnim skupinama odnose prema 8 faktora interesa, relevantnih za izbor zanimanja u području biologije ili u srodnim znanostima. Najveće su razlike interesa u korist biološki zainteresiranih učenika za faktore Uvjetovanost nasljeđem i okolišem te za Reakcije na fizikalne čimbenike. Biološki zainteresirani učenici su selektivniji od ukupnog uzorka ispitanika, a i kod njih interes za predmet biologiju opada s godinama. Izrazita je povezanost izbora profesije u znanstvenom području biologije i afiniteta za predmet Priroda/Biologija (r = 0,87), koju prati i vrlo visoka povezanost variranja iskaza učenika (c = 0,89). Učenici koji se žele baviti nekim oblikom znanstvenog rada u biologiji visoko vrednuju Korisnost predmeta (r = 0,56; c = 0,45) i potvrđuju u svojem iskustvu češće neposredne kontakte s prirodom (r = 0,44; c = 0,39). Ključne riječi: učenički interesi, predmet biologija, izbor zanimanja, biološka znanost th
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STUDENTS' AFFINITIES FOR PROFESSIONAL CARRIER IN THE FIELD OF BIOLOGY D. Garašić1, I. Radanović2, Ž. Lukša3, M. Sertić Perić2 1 Education&Teacher Training Agency, Zagreb, Croatia ([email protected]) 2 Faculty of Science, Division of Biology, Zagreb University, Zagreb, Croatia 3 High School Josipa Slavenskog Čakovec, Čakovec, Croatia We investigated the correlation of students' opting for occupations in the scientific field of biology, with the interest shown and their life experiences. Transversal study was conducted during 2010 on a sample of 2,114 students from 10 to 18 years of age. Affirmative students' responses to the thematic area of Development and achievements of science, with a mean ≥2.8 were taken as an indicator of research curiosity. Compared were the answers given by the different age groups of students interested in science concerning 8 factors, relevant to occupational choice in the field of biology or related sciences. The biggest differences in favour of the interested students were shown for topics dealing with the heritage and the environment, as well as for biological reactions to physical factors. Biologically interested students were more selective in their answers than the total sample. Biologically interested students also show the drop of interest for school subject biology as they are older. Distinct connection between professional choice for scientific work in the field of biology and a distinctive affinity for the subject Biology (r = 0.87) were found. Students who wish to engage in some form of scientific research in biology highly value the usefulness of biology as school subjects (r = 0.56, c = 0.45), and confirmed more frequent direct contacts with nature (r = 0.44, c = 0.39). Key words: students' interests, Biology as school subject, carrier choice, biological scientific area O‐20 UTJECAJ AKTIVNOSTI UPOZNAVANJA BILJAKA NA MOTIVACIJU I ZNANJE UČENIKA Ž. Lukša, T. Šalamon, T. Dragić Runjak, N. Sinković Gimnazija Josipa Slavenskog Čakovec, Čakovec, Hrvatska ([email protected]) U istraživanju se pošlo od pretpostavke da će mogućnost izbora i izražavanja kreativnosti u aktivnosti upoznavanja biljaka kroz izradu radova o biljkama utjecati na motivaciju učenika, a time i na promjenu njihovih stavova o njima te pridonijeti trajnijem usvajanju znanja. U istraživanju je sudjelovalo ukupno po 120 učenika 2., 3. i 4. razreda Gimnazije Josipa Slavenskog Čakovec u šk. god. 2014./15. Učenici 2. razreda ispitivani su prije provedbe zadatka, a učenici 3. i 4. razreda nakon što su u 2. razredu sudjelovali u izradi mape biljaka. Učenici su tijekom ljetnih praznika imali obvezu izraditi mapu biljaka prema vlastitom izboru i mogli su korisiti različite tehnike ili njihovu kombinaciju npr. fotoherbarij, crteže biljaka, prešane biljke, informatičke prikaze i sl. Učenici su uz svaku biljku u mapi prikazivali i dodatne podatke o biljci, također prema svom izboru. Analizirani su radovi učenika, ispitana su njihova znanja te su anketiranjem ispitana mišljenja učenika o provedenoj aktivnosti i vlastitom napretku kroz njih. Učenici 3. i 4. razreda pokazuju statistički značajno bolje rezultate od učenika 1. i 2. razreda. Čak 43% učenika bolje je motivirano za rad zbog mogućnosti izbora načina na koji će obraditi biljke. Preko 66% učenika nakon odrađenog zadaka prepoznaje obrađene biljke u svojoj okolini. Rezultati pokazuje da unatoč početnom nezadovoljstvu zadaćom učenici uočavaju svoj napredak i da su nakon zadatka više opažali biljke u okolišu te govore u prilog postavljene hipoteze o potrebi korištenja različitih oblika rada u nastavi koji potiču aktivnost i motivaciju učenika za trajnije usvajanje znanja. th
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Ključne riječi: aktivno učenje, izbor, mapa biljaka, motivacija THE IMPACT OF ACTIVITIES OF FAMILIARIZING STUDENTS WITH PLANTS ON THE MOTIVATION AND KNOWLEDGE OF STUDENTS Ž. Lukša, T. Šalamon, T. Dragić Runjak, N. Sinković High School Josipa Slavenskog Čakovec, Čakovec, Croatia ([email protected]) The study starts from the hypothesis that a possibility of choice and expression of creativity in the activities of familiarizing students with plants through creating a herbarium will affect the motivation of students, and their attitudes about plants and contribute to a more lasting acquisition of knowledge. The study included 120 students of 2nd, 3rd and 4th grade of high school, during the 2014/2015 The students 2th grade were surveyed prior to the implementation of the task, and others after having created herbaria of plants. The students had to complete a herbarium according to their choice during their summer holidays, and could use different techniques or their combinations, such as photo‐herbarium, drawings of plants, pressed plants, etc. and added additional information with every plant in the herbarium. We analyzed the students herbaria, tested their knowled ge and surveyed their opinions on the performed activities and their own progress through them. Students grade 3 and 4 show significantly better results than students 1st and 2nd grade. Even 43% of students are better motivated to work because of the choice of a way of process plants. Over 66% of students after completing the task recognizes the plants in their environment. Results showed that despite the initial displeasure task students observed their progress and that after the task was seen more plants in the environment and in favor of the hypotheses about the need to use different forms of work in teaching that encourage activity and student motivation for permanent learning. The results collected through interviews show that despite initial displeasure with the assigned task, the students recognize their progress and that after the task has been carried out they notice more plants in their environment, which supports the hypothesis about the need to use different forms of work in teaching which encourage activity and motivation of students for permanent acquisition of knowledge. Key words: active learning, choice, herbarium, motivation O‐21 MOTIVACIJA UČENIKA SEDMIH RAZREDA ZA STJECANJE ZNANJA IZ NASTAVNOG PREDMETA BIOLOGIJA D. Novoselić1, N. Kletečki2, M. Bulić3 1 Alfa d.d., Zagreb, Hrvatska ([email protected]) 2 OŠ Bogumila Tonija, Samobor, Hrvatska 3 OŠ Pujanki, Split, Hrvatska Cilj istraživanja bio je korištenjem 'Upitnika o motiviranosti za učenje biologije' utvrditi jesu li učenici sedmih razreda motivirani za učenje biologije. Uzorak su činili učenici sedmih razreda jedne gradske osnovne škole. Temeljni instrument bio je anketni upitnik koji sadrži trideset čestica zatvorenog tipa na koja su odgovori mogući unutar 5‐stupanjske skale Likertovog tipa. Šest je elemenata unutar kojih su raspoređena pitanja upitnika: intrinzična motivacija, ekstrinzična motivacija, osobna procjena važnosti učenja biologije, lokus unutarnje kontrole, procjena vlastite uspješnosti, anksioznost pri provjeri znanja iz biologije. Ispitanici su ponekad do često motivirani za učenje biologije. Ekstrinzično su motivirani: važna im je dobra ocjena iz biologije no do takve ocjene žele doći uz povremeno učenje. Ne uočavaju povezanost između th
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sadržaja biologije i svakodnevnog života. Učenicima je stalo do postizanja uspjeha, ali su istovremeno obuzeti snažnim strahom od neuspjeha. Uzrok svog uspjeha i/ili neuspjeha učenici percipiraju kao više ili manje podložan njihovoj kontroli. Ključne riječi: motivacija, biologija, ekstrinzična motivacija, intrinzična motivacija, osjećaj uspješnosti, strah od neuspjeha SEVENTH GRADERS’ MOTIVATION FOR KNOWLEDGE ACQUISITION OF ELEMENTARY SCHOOL BIOLOGY D. Novoselić1, N. Kletečki2, M. Bulić3 1 Alfa d.d., Zagreb, Croatia ([email protected]) 2 Bogumil Toni Elementary School, Samobor, Croatia 3 Pujanke Elementary School, Split, Croatia The aim of this research is to determine whether eight grade students are motivated to learn biology by using the 'Motivation to Learn Biology Questionnaire'. The sample consists of seventh‐grade students from an urban elementary school. The main research instrument is a questionnaire containing 30 close‐ended items with a five‐point Likert‐type scale. Items are distributed between six different components: intrinsic motivation, extrinsic motivation, personal relevance of learning biology, self‐determination, personal success assessment, and anxiety about biology assessment. The majority of participants stated they are 'sometimes to often' motivated to learn biology. They do not consider it a challenge. They feel successful when they understand the content of the subject. They are extrinsically motivated: a good grade in biology is important to them but they want to achieve it by learning occasionally. They do not see the connection between the content of biology as a school subject and everyday life. Students care about being successful, but at the same time, they dread failure. Students perceive the cause of their success and/or failure as being more or less under their control. Key words: motivation, biology, extrinsic motivation, intrinsic motivation, feeling of success, fear of failure O‐22 E‐BIOLOGIJA NA ZDRAVSTVENOM VELEUČILIŠTU
A. Mojsović Ćuić1, O. Digula2 1 Katedra za biologiju, Zdravstveno veleučilište, Zagreb, Hrvatska (ana.mojsovic‐[email protected]) 2 Informatička služba, Zdravstveno veleučilište, Zagreb, Hrvatska
Kako bismo studentima omogućili kvalitetnije savladavanje gradiva i olakšali nastavni proces, na Zdravstvenom veleučilištu osmislili smo jedan hibridni nastavni proces koji uz klasične načine poučavanja uključuje i napredne tehnološke istupke. Tako smo predavanja i seminare smjestili na platformu za e‐učenje Moodle te na studentsku platformu za razmjenu datoteka baziranu na Microsoft sharepoint tehnologiji. Studentima smo omogućili konstantan pristup nastavnim materijalima, jednostavniju predaju seminarskih radova te ostvarili jednostavniju i bržu suradnju sa studentima. U vježbovnoj nastavi korištenje samo jednog tipa nastavnog pomagala često ograničava mogućnost izravnog tumačenja određenog gradiva, naročito kada je riječ o staničnoj biologiji. Svjetlosnim mikroskopom, kao osnovnim pomagalom u vježbovnoj nastavi stanične biologije, ne možemo vidjeti stanične organele u citoplazmi eukariotskih organizama. U tom th
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slučaju, osim izrade mikroskopskih preparata, koristimo i aplikacije na tabletima, poput 'Cell World', 'Cell and cell structure', 'Cytogenetics' i 'Gene Screen', koje kao i u slučaju mikroskopa, svaki student dobiva na korištenje za vrijeme nastave. Također, jedan od mikroskopa je opremljen kamerom i povezan sa svim tablet računalima.Treba naglasiti da niti jedno od navedenih pomagala nije zamjena za ono drugo, već isključivo nadopuna i tek njihovom kombinacijom, odnosno zajedničkom primjenom, dobivamo cjeloviti pristup u procesu poučavanja.
Ključne riječi: nastavni proces, biologija, mikroskop, tablet, aplikacije E‐BIOLOGY AT THE UNIVERSITY OF APPLIED HEALTH SCIENCES
A. Mojsović Ćuić1, O. Digula2 1 Department of Biology, University of Applied Health Sciences, Zagreb, Croatia (ana.mojsovic‐ [email protected]) 2 IT Department, University of Applied Health Sciences, Zagreb, Croatia
In order to enable the students to master the subject matter with more quality and in order to ease the teaching process, we have conceived a hybrid teaching process, which in addition to classic ways of teaching also includes advanced technological approaches. So we installed the teaching materials on an e‐study platform Moodle and on the student platform for file exchange, based on Microsoft sharepoint technology. We have enabled the students to have constant access to teaching material, simpler hand over of seminar papers and we have accomplished a simpler and faster collaboration with students. In the practical lessons, the use of only one teaching tool often limits the possibility of direct interpretation of a certain subject topic, especially in cellular biology. By using the light microscope, as a basic tool in practical lessons, we cannot see cellular organelles in the cytoplasm of eukaryotic organisms. In that case, besides making microscopic preparations, we also use applications on tablets, such as 'Cell World', 'Cell and cell structure', 'Cytogenetics' and 'Gene Screen', which are given to each student during classes, the same as in the case of microscopes. Also, one of the microscopes is equipped with a camera and connected to all tablets. It should be emphasized that none of the mentioned tools is a replacement for the other, but exclusively a supplement and we attain a complete approach to the teaching process only through their mutual application. Keywords: teaching process, biology, microscope, tablet, applications O‐23 UPORABA DIGITALNIH NASTAVNIH SADRŽAJA U NASTAVI BIOLOGIJE
A. Belančić Osnovna škola Ivane Brlić‐Mažuranić, Ogulin, Hrvatska ([email protected]) Cilj istraživanja je analiza utjecaja informacijsko‐komunikacijske tehnologije (IKT) na nastavu biologije i prirode u hrvatskim osnovnim školama, s naglaskom na korištenje digitalnih nastavnih sadržaja. Uporabom online upitnika dobiveni su odgovori ukupno 60 učitelja biologije. Dobiveni podaci potvrđuju značajan utjecaj IKT na suvremene nastavne metode u nastavi biologije. Projekti kojima se financijski podupiru škole u nabavci informatičke opreme imaju utječu na uvjete rada 70% ispitanika. Gotovo polovica ispitanika sudjelovala je u nekom od školskih projekata koji su uključivali korištenje IKT. Međutim čak polovica ispitanika tvrdi da o ovoj tematici uči samostalno te ističe nedostatak formalne edukacije. 65% učitelja izjavljuje da koristi digitalni nastavni sadržaj u svakodnevnom radu, ali od toga je veći broj onih koji th
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koriste gotove materija le izdavačkih kuća, a nešto manji broj onih koji materijale nalaze na Internetu ili izrađuju sami. Skup informacija i sadržaja dostupan putem Interneta potencijalno značajno mijenja način rada učitelja u današnje vrijeme. Način njihova korištenja i prilagodba predviđenom nastavnim sadržajima je zahtjevan, a posljedično prespor zadatak za širu zajednicu nastavnika biologije. U svrhu bolje efikasnosti, a i ubrzanja ovog procesa, potrebno je raditi na razmjeni iskustava, dijeljenju digitalnih sadržaja i formalnoj edukaciji učitelja u okviru ideje o cjeloživotnom učenju.
Ključne riječi: digitalni nastavni sadržaj, biologija, osnovna škola
THE USE OF DIGITAL EDUCATIONAL CONTENT IN TEACHING BIOLOGY A. Belančić Ivana Brlić‐Mažuranić Primary School, Ogulin, Croatia ([email protected]) The aim of the research is to analyze the impact of Information and Communication Technologies (ICT) in teaching Biology in Croatian elementary schools, with an emphasis on the use of digital educational content. Online questionnaire was used and responses of a total of 60 teachers of biology were obtained. The obtained data confirm the significant impact of ICT on the modern teaching methods. Projects which provide financial support for the school in the procurement of IT equipment have affected labor conditions 70% of respondents. Nearly half of respondents participated in some of the school projects involving the use of ICT. On this subject teachers learn independently and they emphasize the lack of formal education. 65% of teachers state that they use digital educational content in their daily work, but there is more of those who use the ready‐made materials, and a slightly smaller number of those who search digital content on the Internet or develop it by themselves. The set of information and content available on the Internet potentially significantly changes nowadays teachers methods. The way of their use and adaptation of the planned teaching content is demanding, and consequently slow task for the wider community of teachers. To improve the efficiency and acceleration of this process, it is necessary to work on the exchange of experiences, sharing of digital content and the formal education of teachers in the framework of the idea of lifelong learning. Key words: digital educational content, biology, primary school O‐24 PROJEKTNA TERENSKA NASTAVA NA OBRONCIMA MEDVEDNICE
L. Šarić1, M. Varga2 1 Osnovna škola Remete, Zagreb, Hrvatska ([email protected]) 2 Osnovna škola Retkovec, Zagreb, Hrvatska
Terenska nastava kao oblik učioničke nastave pedagoški je učinkovita i zanimljiva praksa. Nastavni sadržaji predočeni kao primarni izvor znanja postaju vidljivi i opipljivi, a samim time i zanimljiviji, što uzrokuje njihovo lakše pamćenje. Okoliš Medvednice pruža mnogobrojne mogućnosti za otkrivanje i razumijevanje svijeta u kojem jesmo. Cilj je projekta motivirati i osvijestiti učenike o važnosti očuvanja prirodne baštine. Projekt je zamišljen kao višegodišnji (započet u svibnju 2014. godine) kroz koji se učenicima ukazuje na promjene u vodenom svijetu nastale djelovanjem prirode i čovjeka. Učenici su nosioci svih aktivnosti u sve tri etape terenske th
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nastave pri čemu je naglasak stavljen na praktičan rad na terenu – određivanje različitih parametara vode metodama i načinima prilagođenim kognitivnim i psihomotoričkim sposobnostima dane učeničke dobi. Sve se etape izvode metodom grupnog rada polustrukturiranim zadacima koji obuhvaćaju kemijsku analizu vode potoka, odnosno određivanje kloridnih, fosfatnih, nitratnih, nitritnih te amonijevih iona u vodi, tvrdoće, pH i temperature vode, slobodnog i vezanog CO2, brzine protoka te YTT‐test. Projektna terenska nastava kao složeni oblik praktičnog, misaonog i neposrednog stjecanja znanja potiče kod učenika intelektualnu radoznalost, što utječe na međuvršnjačku (znanstvenu) komunikaciju te stvaranje kvalitetnih odnosa među samim učenicima te između nastavnika i učenika.
Ključne riječi: projektna nastava, terenska nastava, motivacija, zaštita prirode
PROJECT FIELD CLASSES ON THE SLOPES OF MEDVEDNICA L. Šarić1, M. Varga2 1 Primary School Remete, Zagreb, Croatia ([email protected]) 2 Primary School Retkovec, Zagreb, Croatia Field classes as a form of teaching process are an educationally effective and interesting practice. The curricula content presented as a primary knowledge source become visible and tangible, and in turn more interesting, which makes them easily memorable.The natural resources of Medvednica Hill offer a variety of possibilities for discovering and understanding the world we live in. The objective of the project is to motivate students and to make them aware of the importance of conservation of natural heritage. The project started in May of 2014 and it is envisioned as a several‐year long productive project through which students discover the changes in the aquatic world that have occurred due to natural and man‐caused effects. Students are the main contributors in the activities and the stress is on practical work, i.e. determining various properties and parameters of creek water methods tailored to the students’ age in the cognitive and psychomotor sense. All the work stages are implemented by means of group work on semi‐ structured tasks comprising a chemical and physical analysis of the creek water. The project field classes, as a complex practical, cognitive and direct knowledge acquisition process, arouse the intellectual curiosity in students, which in turn influences not only the quality of inter‐coeval (scientific) communication, but also enhances relationships and camaraderie among the students themselves. Keywords: project strategies, field classes, motivation, environmental protection O‐25 ANALIZA STAVOVA I INTERESA UČENIKA SREDNJIH ŠKOLA O PRIRODNIM VRIJEDNOSTIMA MEĐIMURSKE ŽUPANIJE M. Cindrić1, D. Crnčec2, M. Mesarić3 1 Srednja škola Čakovec, Čakovec, Hrvatska ([email protected]) 2 OŠ Sv. Martin na Muri, Sv. Martin na Muri 3 Međimurska priroda ‐ Javna ustanova za zaštitu prirode, Mursko Središće Zadaća ekološke edukacije je prenijeti poruku o značaju i nužnosti očuvanja prirodnih i drugih vrijednosti, a najmoćnijim sredstvom ekološke edukacije smatra se interpretacija. Već je otac interpretacije Freeman Tilden istaknuo: 'Interpretacijom do razumijevanja, razumijevanjem do th
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poštovanja, poštovanjem do zaštite'. Korištenjem izravnih i neizravnih oblika interpretacije, od strane djelatnika Međimurske prirode‐JU za zaštitu prirode i dvoje nastavnika osmišljen je edukativni program Mladi čuvari prirode. Predmetni odgojno‐obrazovni program polazilo je četerdesetak učenika šestih razreda osnovnih škola Međimurske županije. Program se realizirao kroz predavanja, radionice i terenski rad tijekom proljeća 2009. godine s ciljem ekološke edukacije učenika o prirodnim vrijednostima Međimurske županije i povećanjem interesa za prirodoslovlje. Cilj nam je u ovom radu utvrditi utjecaj programa Mladi čuvari prirode na razvoj pozitivnijih stavova o zaštiti prirode na području Međimurske županije te povećanju interesa za prirodoslovlje. Stavovi učenika ispitani su online anketom. U anketiranju su sudjelovali polaznici programa Mladi čuvari prirode 2009. godine, a kao kontrolna skupina njihovi vršnjaci koji nisu sudjelovali u programu. Za analizu ankete primijenjene su neparametrijske statističke metode budući da je riječ o malom uzorku. U anketi se, osim stavova, ispituju i interesi iz područja prirodoslovlja s posebnim naglaskom na zaštićene dijelove prirode. Ključne riječi: prirodoslovlje, prirodne vrijednosti, čuvari prirode, stavovi MIDDLE SCHOOL STUDENTS´ ATTITUDES AND INTERES ANALYSIS ABOUT THEIR NATURAL VALUES IN THE MEĐIMURJE COUNTY M. Cindrić1, D. Crnčec2, M. Mesarić3 1 High School Čakovec, Čakovec, Croatia ([email protected]) 2 Primary school Sv. Martin na Muri, Sv. Martin na Muri, Croatia 3 Međimurje Nature ‐ Public Institution for the Protection of Nature, Mursko Središće Croatia The task of ecological education is to convey a message about the importance and necessity of preserving natural and other values, and the most powerful tool for ecological education is considered to be the interpretation. The father of interpretation, Freeman Tilden said: „Through interpretation, understanding; through understanding, appreciation; through appreciation, protection.“ Employees of Međimurska priroda (Međimurje nature), and two teachers worked out, using direct and indirect forms of interpretation, an educational program called Young guardians of nature. Fourty pupils from the sixth grade from all primary schools of the Međimurje county attended the educational program. The program was realised through a series of lectures, workshops and field work which took place during the spring of 2009 with a goal of ecological education and increasing publi c interest for natural sciences. The goal in this paper is to determine the influence of the program Mladi čuvari prirode on the development of a more positive attitude on the protection of nature in the Međimurje county aswell as increasing the public interest for natural sciences. Students took an online survey and gave their opinion. Participants of the Mladi čuvari prirode 2009 took the survey, while their peers were the control group, who didn't participate in the mentioned program. Except viewpoints, the survey tested interests for natural sciences with special emphasis on protected parts of nature. Key words: natural science, natural values, Young guardians of nature, attitudes O‐26 RAZVOJ JEZIČNIH, DRUŠTVENIH I UPRAVLJAČKIH KOMPETENCIJA U UČENIČKIM ISTRAŽIVAČKIM PROJEKTIMA U PROGRAMU GLOBE I BIOLOGIJI M. Labaš Horvat, M. Rašan th
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Srednja škola Prelog, Prelog, Hrvatska ([email protected]) Srednja škola Prelog je 12 godina uključena u GLOBE program i u okviru GLOBE‐a i izborne nastave biologije odrađeni su brojni učenički istraživački projekti vezani uz prirodne ekosustave u okruženju (jezero, rijeka, travnjak, šuma) koje učenici rade u paru ili grupi od 3 učenika. Za njihovu su izradu potrebne različite komunikacijske, socijalne i druge kompetencije. Učenici pri proučavanju uočenog problema i odabiru metoda za rješavanje istraživačkog pitanja koriste literaturu na engleskom jeziku. Cilj istraživanja je pronaći odgovarajuće alate za mjerenje jezičnih i upravljačkih vještina prije i nakon izrade projekta te odabranim alatima mjeriti društvene kompetencije u različitim fazama izrade projekta ispitivane skupine učenika (koji rade istraživačke radove) u odnosu na kontrolnu skupinu (koji ne rade istraživačke radove). Pretpos tavlja se da će ispitivana skupina mjerenjem navedenih kompetencija tijekom istraživanja pokazati bolje rezultate. Za mjerenje jezičnih kompetencija su korištene CEFR tablice te upitnici za ispitivanje društvenih i upravljačkih kompetencija. Rezultati istraživanja su sljedeći: učenici uključeni u projekte su pokazali izniman napredak unutar CEFR tablice, društvene vještine su uvelike poboljšane, učenici su naučili dijeliti zaduženja te su razvili brojne upravljačke vještine, kao što su prezentacija, javno govorenje, donošenje odluka i pregovaranje. Ključne riječi: jezične, društvene i upravljačke kompetencije DEVELOPMENT OF LINGUISTIC, SOCIAL AND MANAGEMENT COMPETENCIES IN GLOBE AND BIOLOGY STUDENT RESEARCH PROJECTS M. Labaš Horvat, M. Rašan High School Prelog, Prelog, Croatia ([email protected]) Secondary school Prelog has been involved in GLOBE for 12 years and a number of student research projects connected to nearby natural ecosystems have been done by students in pairs or groups of three. In order to work on the project, students need various communicative, social and other competencies. While researching the noticed problem and deciding on the methods to solve the research question, students search through the English literature. The goal of the research is to find adequate tools to measure linguistic and management skills before and after the project, as well as to measure social competencies by using chosen tools in different stages of the project of the questioned group of students (working on projects) compared to the contolled group (not working on projects). Iti is assumed that the questioned group will show better results of the measured competen cies during the research. CEFR grids were used to measure linguistic competencies, and questionaires for measuring social and management competencies. The results of the research are: students involved in projects have shown amazing progress within CEFR grid, their social skills were also enhanced and they have learned to share responsibilities and have also developed various management skills, such as presentation, public speaking, decision making and negotiating. Key words: linguistic, social and menagement skills O‐27 PREDSTAVLJANJE REZULTATA POTPROJEKATA ISTRAŽIVANJE I REKREACIJA U PRIRODI I NASTAVNE METODE U BIOLOGIJI I KEMIJI U OKVIRU PROJEKTA AFIRMATIVNA NASTAVA I INOVATIVNO UČENJE I POUČAVANJE U GIMNAZIJAMA U OKVIRU HRVATSKOG KVALIFIKACIJSKOG OKVIRA ‐ IPAQ PETA th
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Z. Pongrac Štimac, R. Halapir Franković V. gimnazija, Zagreb, Hrvatska ([email protected]) V. gimnazija je bila nositelj projekta IPAQ Peta, koji su osmislili njeni profesori, a proveden je u suradnji s partnerima: PMF‐om, školama iz Vukovara, Metkovića, Pakraca i Knina te suradnikom, AZOO. Kroz sedam potprojekata, namijenjenih nastavnicima, željeli smo razviti i modernizirati obrazovni sustav i kvalifikacije u gimnazijama; modernizirati postojeće i razviti napredne kurikule za gimnazije temeljene na ishodima učenja i razviti nastavne kapacitete za provedbu inovativnog učenja i poučavanja. Potprojektom Istraživanje i rekreacija u prirodi izradili smo kurikul koji sadržajima obuhvaća biologiju, kemiju, geologiju i tjelesnu i zdravstvenu kulturu. Nekoliko znanstvenih metoda istraživanja u prirodi prilagođeno je učenicima i nastavnicima te je osmišljena kvalitetna rekreacija u prirodi. Potprojektom Nastavne metode u biologiji i kemiji izradili smo kurikul koji se dijelom nadovezuje na terensku nastavu, a dijelom već poznate metode usmjerava na učenika i nastoji ostvariti pozitivan i odgovoran stav prema zdravlju, živom svijetu, prirodi i znanosti. Najvažniji cilj ovih potprojekata je promijeniti položaj učenika i položaj nastavnika u nastavnom procesu. Nastavnikovu ulogu predavača želimo promijeniti u ulogu organizatora, menadžera, mentora, instruktora i suradnika, a učenike želimo iz pasivnih promatrača promijeniti u angažirane sudionike nastave, osamostaliti ih u učenju, dodatno ih motivirati i poticati na suradnju i preuzimanje određene odgovornosti. Ključne riječi: kurikul, ishodi učenja, Hrvatski kvalifikacijski okvir PRESENTING THE RESULTS OF SUBPROJECTS RESEARCH AND RECREATION IN NATURE AND TEACHING METHODS IN BIOLOGY AND CHEMISTRY IN THE FRAMEWORK OF AFFIRMATIVE AND INNOVATIVE LEARNING AND TEACHING IN GYMNASIUMS WITHIN THE CROATIAN QUALIFICATIONS FRAMEWORK ‐ IPAQ PETA Z. Pongrac Štimac, R. Halapir Franković Fifth Gymnasium, Zagreb, Croatia ([email protected]) Fifth Gymnasium was the project leader of IPAQ Peta, which was conceived by its professors and implemented in cooperation with the Faculty of Science, four schools from Vukovar, Metković, Pakrac and Knin and AZOO (Education and Teacher Training Agency). In our project, consisting of 7 subprojects, we wanted to develop and modernize the teaching system and advance qualifications in grammar schools; modernize the existing and develop advanced curricula for grammar schools based on the proper use of learning outcomes, and develop teaching capacities for the implementation of student–centred learning. Within the subproject Research and recreation in nature, we have created a curriculum that includes the fields of biology, chemistry, geology and physical education. Several scientific research methods in nature are adapted to students and teachers and it also contains quality recreation in nature. Within the subproject Teaching methods in biology and chemistry, we have created a curriculum consisting of well‐known methods directed at students and aiming to achieve a positive and responsible attitude towards health, the living world, nature and science. The most important objective of this subproject is to change the position of the students and the role of teachers in the teaching process. We want to transform the teachers' role of trainers into the role of organizers, managers, mentors, instructors and assistants, and the students' role of passive observers into engaged participants, teaching them to become independent learners and further motivate and encourage them to cooperate and take some responsibility. th
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Keywords: curriculum, learning outcomes, Croatian Qualifications Framework O‐28 PRIMJER INTEGRIRANJA RAZLIČITIH NASTAVNIH PREDMETA NA TEMU ZAŠTITE LOKALITETA BEDEKOVIĆEVE GRABE Ž. Kadi1, J. Pavlic2 1 Ekonomska i trgovačka škola Čakovec, Čakovec, Hrvatska ([email protected]) 2 Osnovna škola Ivana Gorana Kovačića Sveti Juraj na Bregu, Lopatinec, Hrvatska Na osnovi rezultata istraživanja bioloških osobitosti leptira iz roda Maculinea na lokalitetu Bedekovićeve grabe u Međimurju osmišljene su i provedene višegodišnje edukativne aktivnosti radi očuvanja i zaštite staništa velikog livadnog plavca Maculinea teleius Brgstr. i zagasitog livadnog plavca Maculinea nausithous Brgstr. Cilj rada je aktivnim uključivanjem u interdisciplinarne projekte senzibilizirati učenike različitog uzrasta i područja obrazovanja za očuvanje ugroženih i zaštićenih livadnih plavaca u Međimurju. Aktivnosti se provode uz potporu Županijskog ogranka HPKZ‐a Međimurske županije, Međužupanijskog stručnog vijeća nastavnika biologije, i udruge 'Ekološka škola'. Ostvareno je integriranje različitih nastavnih predmeta u osnovnoj školi (1.‐8. razreda) i projekata učenika srednjih škola ovisno o njihovom području interesa; ekologija, ekonomija, trgovina, turizam i ugostiteljstvo. Uključeni su učenici OŠ I. G. Kovačića Sv. Juraj na Bregu, Ekonomske i trgovačke škole Čakovec, Gimnazije Josipa Slavenskog Čakovec, SŠ Prelog, Gospodarske škole Čakovec i njihovi nastavnici u skladu s obrazovnom razinom i sektorom. U drugom djelu rada se, na temelju provedbe i rezultata navedenih aktivnosti u školama anketiranjem izabranog uzorka učenika, istražuju njihovi stavovi i kompetencije, posebice poduzetničke i prirodoznanstvene. Ključne riječi: leptiri roda Maculinea, očuvanje staništa, interdisciplinarna i međugeneracijska edukacija, stavovi AN EXAMPLE OF INTEGRATING DIFFERENT SCHOOL SUBJECTS FOCUSING ON PROTECTING THE SITE 'BEDEKOVIĆEVE GRABE' Ž. Kadi1, J. Pavlic2 1 Secondary School of Economics and Trade Čakovec, Čakovec, Croatia ([email protected]) 2 Primary School Ivan Goran Kovačić Sv. Juraj na Bregu, Lopatinec, Croatia Based on the results of the research into the biological features of the butterflies of the species Maculinea at the site 'Bedekovićeve grabe' in Međimurje many long lasting educational activities have been devised and implemented with the aim of preservation and protection of the habitat of the scarce large blue butterfly Maculinea teleius Brgstr. and the dusky large blue butterfly Maculinea nausithous Brgst. The aim of the activities was to make students of different age and educational sector more aware of the need to preserve endangered and protected large blue butterflies in Međimurje by their active involvement in interdisciplinary projects. The results achieved were the integration of various school subjects in primary school (grade 1 to grade 8) and secondary school students' projects which depended on their area of interest: ecology, economics, trade, tourism and hotel industry. The participants involved were students and teachers from Primary School Ivan Goran Kovačić, Sv. Juraj na Bregu; Secondary School of Economics and Trade, Čakovec; Secondary School Prelog; Grammar School J. Slavenskog Čakovec and Vocational School Čakovec. Using the results of the implemented th
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activities, the second part of the study focuses on conducting the sample survey into the students’ attitudes and competence, particularly entrepreneurial and scientific competence. Keywords: butterflies of the species Maculinea, protection of habitat, interdisciplinary and intergenerational education, attitudes O‐29 ISTRAŽIVANJE STAVOVA UČENIKA O PREHRANI N. Graovac, E. Merdić Odjel za biologiju, Sveučilište Josipa Jurja Strossmayera, Osijek, Hrvatska ([email protected]) Osnovni cilj ovoga rada bio je saznati prehrambene i druge navike, stavove i aktivnosti učenika, te utvrditi postoje li razlike među učenicima ovisno o spolu, dobi i sredini u kojoj žive. Istraživanje je provedeno putem upitnika konstruiranog za potrebe ovog istraživanja. U istraživanju je sudjelovao 651 učenik. Najveći broj učenika je normalne tjelesne mase, 481 (73,88%). Uhranjenost dječaka i djevojčica se razlikuje. Učenici ruralne sredine doručkuju redovitije (p=0,010), češće jedu perad (p=0,008) te domaće suhomesnate proizvode (p=0,003), dok učenici urbane sredine češće jedu ribu (p=0,044) i cjelovite žitarice (pF9>F4>F2>F8.
Ključne riječi: PAH, ribe, Jadransko more, nafta, onečišćenje
BILE PAH METABOLITES CONTENT IN SEVERAL COMMERCIAL FISHES ALONG EASTERN ADRIATIC COAST
B. Hamer1, A. Baričević1, N. Medić2, V. S. Perusco1 1 Ruđer Bošković Institute, Center for Marine Research, Rovinj, Croatia ([email protected]) 2 Juraj Dobrila University of Pula, Pula, Croatia
Bile PAH metabolite content in selected commercial species Mugil cephalus, Sarpa salpa and Mullus barbatus purchased from local fish markets: Ploče (F2), Split (F4), Šibenik (F5), Rijeka (F8) and Rovinj (F9) were analyzed as a biomarker of exposure to oil and possible indicator of marine environment quality. Local fish markets were used because it was not possible to cath chosen species at sites investigated in the frame of pilot Project Adriatic II. Fishes were selected according food web positions: M. cephalus (water column, omnivore), S. salpa (water column, herbivore) and M. barbatus (benthic, detritofag). The analysis of cox1 gene was applied as tool for fish specimens’ identification (barcoding). PAH metabolites were analyzed in bile by means of fixed wavelength fluorescence (FF) measurement using fluorimeter excitation/emission wavelength pairs 290/335, 341/383, and 380/ 430 nm. By FF290/335, mainly naphthalene type of metabolites, typically associated with petroleum products are detected, benzo[a]pyrene type of metabolites are best detected by FF380/430 and by FF341/383 mainly pyrene‐derived metabolites. Results indicate mostly naphthalene‐ type fish exposure cca. 10‐30 times higher values for FF290/335 (304±91), than two other types FF341/383 (24±6) and FF380/430 (14±4). Further, total sum values of all three fish species ranked fish contaminant exposure at investigated areas as follows: F5>F9>F4>F2>F8.
Key words: PAH metabolites, fish, Adriatic Sea, oil, pollution
O‐113 ALERGENI GRINJA, ŽOHARA I PLIJESNI U SEDIMENTIRANOJ PRAŠINI U KUĆANSTVU U HRVATSKOJ Lj. Prester, J. Macan th
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Jedinica za medicinu rada i okoliša, Institut za medicinska istraživanja i medicinu rada, Zagreb, Hrvatska ([email protected]) Svrha rada bila je ustanoviti razine alergena unutarnjih prostora koji su povezani sa zdravstvenim rizicima. U sedimentiranoj prašini odredili smo koncentracije alergena: grinja Dermatophagoides pteronyssinus (Der p1) i Dermatophagoides farinae (Der f 1), žohara Blatella germanica (Bla g 1), te plijesni Alternaria alternata (Alt a 1) i Aspergillus fumigatus (Asp f 1). Razina alergena grinja Der p 1 i Der f 1 veća od 2 μg/g prašine povezana je s alergijskom senzibilizacijom, a razina veća od 10 μg/g s kliničkim respiratornim simptomima. Sakupljeno je 30 uzoraka sedimentirane prašine uzorkovane u kućanstvima sa zagrebačkog područja. Nakon ekstrakcije, alergeni su određeni standardnom enzim‐imunokemijskom metodom (ELISA) (Indoor Biotechnologies Ltd, Cardiff, UK). Alergeni grinje ustanovljeni su u 75% kućanstava. Koncentracija Der p 1 bila je u rasponu od 0,1‐ 12,5 μg/g, a Der f 1 od 0,10‐3,2 μg/g. Koncentracija Der p 1 + Der f 1 > 2 μg/g, povezana s povećanim rizikom za senzibilizaciju, ustanovljena je u 27% kućanstva, a Bla g 1 u manje od 3% kućanstva. Alergeni plijesni Alt a 1 i Asp f 1 nisu ustanovljeni niti u jednom uzorku kućne prašine (ispod granice detekcije metode). Zaključuje se da su alergeni grinja najčešći alergeni u kućanstvima zagrebačkog područja. Ključne riječi: alergeni unutarnjih prostora, grinje kućanske prašine, žohari, plijesni, Der p 1 ALLERGENS OF MITES, COCKROACHES AND MOULDS IN SETTLED DUST IN HOUSHOLDS IN CROATIA Lj. Prester, J. Macan Occupational Health and Environmental Medicine Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia ([email protected]) We evaluated the level of main allergens of indoor environment associated with health effects in man. We have determined the level of dust mites allergens Der p 1 (Dermatophagoides pteronyssinus) and Der f 1 (Dermatophagoides farinae), cockroach allergen Bla g 1 (Blatella germanica), and mould allergens Alt a 1 (Alternaria alternata) and Asp f 1 (Aspergillus fumigatus). For mite allergens Der p 1 and Der f 1 a threshold levels of 2 and 10 μg/g of dust have been proposed for development of allergic sensitization and respiratory symptoms, respectively. A total of 30 dust samples were collected in urban homes in Zagreb. After extraction, all allergens were detected by using standard enzyme immunoassays (ELISA) (Indoor Biotechnologies Ltd, Cardiff, UK). Mite allerges were detected in 75% of households. The concentration of Der p 1 and Der f 1 were in the range 0.1‐12.5 μg/g and 0.10‐3.2 μg/g, respectively. While the levels of Der p 1 + Der f 1 >2 μg/g associated with increased risk of sensitization were found in 27% of homes, this risk was found in less than 3% of dust samples for cockroach allergen Bla g 1. Mold allergens Alt a 1 and Asp f 1 were not detected in any dust samples (lower than limit detection of method). The results suggest that mite allergens are the most common indoor allergens in households in Zagreb area. Key words: indoor allergens, house dust mites, cockroaches, moulds, Der p 1 O‐114 SINERGISTIČKI UČINCI VAZODILATACIJSKIH I ANTIKOAGULACIJSKIH LIJEKOVA NA OKSIDATIVNI STRES U MOZGU J. Krasić, D. Đikić th
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Zavod za animalnu fiziologiju, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) Prethodna toksikološka istraživanja ukazuju da su krvno moždana barijera i krvotok unutar središnjeg živčanog sustava vrlo osjetljivi na učinke lijekova s vazodilatacijskim ili antikoagulativnim svojstvima. Dobro su opisani štetni učinci oksidativnog stresa na staničnoj razini uzrokovanog lijekovima uobičajenima u domaćinstvu, koji se uzimaju bez recepta (acetilsalicilna kiselina) te biljnih lijekova i tableta (npr. ginko) ili lijekova na recept s antikoagulativnim svojstvima (varfarin). Mnogo manje se zna o međusobnim interakcijama navedenih tvari na razine oksidativnog stresa, osobito u mozgu. U okviru ove studije Y59 štakori tretirani su dnevno s odgovarajućim preporučenim dozama i kombinacijama acetilsalicilne kiseline, ginka i varfarina, tijekom 15 dana. Razina oksidativnog stresa procijenjena je mjerenjem četiri markera oksidativnog stresa; superoksid dismutaze (SOD), katalaze (CAT), glutationa (GSH) i malondialdehida (MDA) u različitim regijama mozga. Rezultati su pokazali promjenjene razine oksidacijskog stresa u prefrontalnom korteksu, kortikalnoj regiji i malom mozgu izloženih životinja. Ove promjene ukazuju na sinergistički toksični potencijal izazvan simultanim uzimanjem navedenih lijekova. Ključne riječi: mozak, oksidativni stres, antikoagulativni i vazodilatacijski lijekovi SYNERGISTIC EFFECTS OF VASODILATATIONAL AND ANTICOAGULATIVE DRUGS ON BRAIN OXIDATIVE STRESS J. Krasić, D. Đikić Division of Animal Physiology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) Previous toxicological research showed that blood brain barrier and brain vascular system are highly susceptible to the effects of pharmaceuticals with vasodilatation or anticoagulative properties. The adverse effects on cellular oxidative stress caused by over the counter (OTC) of household drugs (acetylsalicylic acid), herbal remedies and pills (for example gingko) or prescription drugs (warfarin) are well described. Much less is known about mutual interaction between these substances on oxidative stress levels especially in the brain. Within this study Y59 rats were treated daily with corresponding recommended doses and combinations of acetylsalicylic acid, ginkgo and warfarine during 15 days. Four markers of oxidative stress were assessed; superoxide dismutase (SOD), catalase (CAT), gluthathione (GSH) and malondialdehyde (MDA) in various brain regions. The results have shown changes of oxidative stress levels in prefrontal cortex, cortical regions and cerebellum of exposed animals. These changes implicate synergistic and potentiated toxic potential of combinations of named drugs. Key words: brain, oxidative stress, vazodilatational and anticoagulative drugs th
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Mikrobiologija Microbiology O‐115 KOMPARATIVNA ANALIZA GENOMA FITOPLAZMI: OD NESTABILNOSTI GENOMA DO PRILAGODBE NA DOMAĆINE M. Šeruga Musić, A. Slović, M. Pinterić Zavod za mikrobiologiju, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) Fitopatogene bakterije roda 'Candidatus Phytoplasma' malih su genoma koji su kroz dinamičnu koevoluciju s biljnim domaćinima i kukcima‐vektorima doživjeli specifične gubitke i dobitke gena. Još uvijek ih nije moguće uzgojiti u čistoj kulturi in vitro. Unatoč tome, u potpunosti su sekvencirana i dostupna 4 fitoplazmatska genoma uz nekolicinu nepotpunih. Iako reducirani, genomi fitoplazmi bogati su ponavljajućim sljedovima. Ponavljajući sljedovi nazvani „potencijalne pokretne jedinice“ (potential mobile units; PMUs) nalik su replikativnim transpozonima i mogu postojati kao kružni ekstrakromosomski elementi s povećanom ekspresijom u kukcima u odnosu na biljke. PMUs doprinose varijacijama u veličini te visokoj plastičnosti genoma i prilagodbi na različite domaćine. Jedan od ponavljajućih gena jest i gen ssb, Samo jedna kopija gena ssb u genomu im a drugačije evolucijsko porijeklo, nalazi se u specifičnom genomskom okruženju te se smatra izvornom kopijom. Izvorna kopija gena ssb izolata vrsta 'Ca. P. asteris' i 'Ca. P. solani' umnožena je i sekvencirana. Filogenetskom analizom pokazana je očuvanost izvorne kopije gena ssb, uz izuzetak vrste 'Ca. P. mali' što je u skladu s posebnom organizacijom i stabilnošću genoma ove fitoplazme. Metodom Southern blot, u genomu 'Ca. P. solani' dokazana je prisutnost još jedne kopije tog gena, što ukazuje na mogućnost postojanja ekstrakromosomskog elementa karakterističnog za ovu vrstu čiji je genom najveći i najnestabilniji među fitoplazmama. Ključne riječi: fitoplazma, genomika, filogenija, genom, biljni patogen COMPARATIVE ANALYSIS OF PHYTOPLASMA GENOMES: FROM GENOME INSTABILITY TO HOST ADAPTATION M. Šeruga Musić, A. Slović, M. Pinterić Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) Insect‐transmitted bacteria from the genus 'Candidatus Phytoplasma' have small genomes that experienced specific gene losses and gains through their dynamic co‐evolution with plant and insect hosts. Axenic cultivation of these bacteria is still challenging. However, a number of genome drafts and 4 fully annotated genomes are available. In spite of being reduced, phytoplasma genomes are repeat‐rich and contain multicopy genes. Repeats termed potential mobile units (PMUs) have characteristics of replicative transposons and may exist as circular extrachromosomal elements with enhanced expression in insects compared with plant hosts. PMUs contribute to phytoplasma genome size variations and high plasticity as well as to adaptation to diverse hosts. One of the multicopy genes is the ssb gene with only one copy located in a specific genomic neighbourhood having a diffe rent evolutionary origin. In this study, ssb gene was amplified and sequenced from a number of 'Ca. P. asteris' and 'Ca. P. solani' th
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isolates. Phylogenetic analyses have shown that all “original” ssb genes from different phytoplasmas clustered together. The exception was the 'Ca. P. mali' single ssb gene, which is in accordance with its' different genome organization and stability. Southern blot analyses have revealed another copy of the ssb gene in 'Ca. P. solani', suggesting the presence of an extrachromosomal element characteristic of the species having the largest and the most unstable genome among phytoplasmas. Key words: phytoplasma, plant pathogen, genomics, genome, phylogeny O‐116 MOLEKULARNA TIPIZACIJA UZROČNIKA FILODIJE ULJANE REPICE D. Škorić, M. Šeruga Musić, S. Černi Biološki odsjek, Prirodoslovno‐matematički fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska ([email protected]) Filodija uljane repice (Brassica napus ssp. oleifera (DC.) Metzg.) je bolest koja izaziva velike gubitke i do sada je bila povezivana sa zarazom fitoplazmama (rod 'Candidatus Phytoplasma'). Biljke prikupljene na području Zagreba imale su, tipično za sindrom filodije, virescenciju, male deformirane mahune i reduciran broj sjemenki. Također su zabilježene nekroze stabljika i listova, te kloroze listova tipične za infekciju virusom mozaika repe (Turnip mosaic virus; TuMV). Kako bi se otkrio uzročnik bolesti, provedena je molekularna tipizacija fitoplazmi i virusa. Biološka i molekularna istraživanja otkrila su infekciju TuMV‐om, a izolat CRO184A klasificiran je u skupinu „world‐B“. Filogenija nije pokazala tragove rekombinacija. PCR‐RFLP analizom i sekvenciranjem 16S rRNA očekivano je potvrđena prisutnost fitoplazme 'aster yellows' (AY, 'Ca. P. asteris'), soja iz ribosomske podskupine 16SrI‐B. Multigenska analiza konzerviranih gena tufB, secY, groEL i ribosomskih proteinskih gena te varijabilnog gena amp potvrdila je klasifikaciju fitoplazme. Gen amp kodira AY‐specifični imunodominantni membranski protein, a promjene u njemu ukazuju na drukčiju prenosivost kukcima‐vektorima tj. prilagodbe novom tipu vektora. Molekularnom tipizacijom po prvi puta je detaljno opisana fitoplazma‐uzročnik filodije uljane repice, a također je prvi put dokazana koinfekcija repice fitoplazmom i virusom. Ova studija predstavlja osnovu za daljnja istraživanja njihovih sinergističkih učinaka. Ključne riječi: 'Candidatus Phytoplasma asteris', genom, MLST, virus mozaika repe MOLECULAR TYPING IN DECIPHERING ETIOLOGY OF RAPE PHYLLODY DISEASE D. Škorić, M. Šeruga Musić, S. Černi Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) Rape phyllody disease associated with phytoplasmas can decimate the oilseed rape (Brassica napus ssp. oleifera (DC.) Metzg.) production. Plants sampled in Zagreb area had typical rape phyllody syndrome including virescence, small deformed siliques and seed reduction. Also, atypical stem necrosis, leaf chlorotic spots and necrotic changes reminiscent of a Turnip mosaic virus (TuMV) infection were recorded. Molecular typing was performed aimed at characterizing agents involved in this rape phyllody outbreak. Biological and molecular investigations revealed the infection with TuMV and the isolate CRO184A was classified into the world‐B phylogenetic th
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lineage. No evidence for interlineage recombination was obtained. PCR‐RFLP and 16S rRNA gene sequencing identified an aster yellows phytoplasma (AY, 'Candidatus Phytoplasma asteris') strain of the 16SrI‐B subgroup. Multig ene sequence characterization of house‐keeping tufB, secY, groEL and ribosomal protein genes plus a highly variable amp gene confirmed its affiliation. The AY specific amp gene encoding an immunodominant membrane protein showed significant variability suggesting altered insect vector specificity and transmissibility. Molecular typing provided the first detailed characterization of 'Ca. P. asteris' strain associated with the rape phyllody disease. Also, this was the first recorded 'Ca. P. asteris' and TuMV co‐infection in oilseed rape. This research lays foundation for investigating their possible synergistic effects. Key words: 'Candidatus Phytoplasma asteris', MLST, Turnip mosaic virus, genome O‐117 RAZNOLIKOST GLJIVE Cryphonectria parasitica U ZARASTAJUĆIM KALUSIMA KESTENA V. Karin1, M. Ježić1, M. Idžojtić2, M. Ćurković Perica1 1 Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) 2 Zavod za šumarsku genetiku, dendrologiju i botaniku, Šumarski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska Cryphonectria parasitica je patogena gljiva koja uzrokuje rak kestenove kore. Zaraženi pitomi kesteni mogu se oporaviti prirodnim putem ukoliko se gljiva koja je uzrokovala rak zarazi hipovirusom Cryphonectria hypovirus 1 (CHV1). Time dolazi do konverzije gljive u hipovirulentnu, što omogućuje biološku kontrolu bolesti. CHV1 kao genom ima dsRNA, čija prisutnost u miceliju reducira virulenciju i reproduktivni kapacitet gljive. Ukoliko se CHV1 unese u aktivni rak uzrokovan virulentnim sojem gljive C. parasitica, prestaje širenje raka te se formira kalus – zarastajući rak. Zbog toga je važno da hipovirulentni sojevi opstaju u populaciji gljive C. parasitica. Cilj ovog istraživanja bio je ustanoviti raznolikost sojeva gljive C. parasitica u zarastajućim kalusima kestena u populacijama iz Ozlja i Markuševca u Zagrebu. Uzorci kore prikupljeni su s 24 zarastajuća raka kestena i to iz kalusnog tkiva i tkiva na granici kalusa i debla. Iz uzoraka kore izolirana je čista kultura gljive C. parasitica. Prisutnost CHV1 provjerena je izolacijom hipovirusne dsRNA i RT‐PCR‐om. Zanimljivo je da smo često nalazili virulentne sojeve gljive C. parasitica u zarastajućim rakovima, iako hipovirulentni sojevi potiču zarastanje. Pretpostavljamo da se to moglo dogoditi ili zbog toga što se soj koji je uzrokovao zarastanje, nakon nekog vremena spontano oslobodio od virusa, ili zbog naseljavanja novih, virulentnih, sojeva gljive koji bi ponovno mogli izazvati bolest na već zaraslim rakovima. Ključne riječi: Cryphonectria parasitica, rak kestenove kore, Cryphonectria hypovirus 1 (CHV1), zarastajući rak DIVERSITY OF Cryphonectria parasitica IN HEALED CHESTNUT CANKERS V. Karin1, M. Ježić1, M. Idžojtić2, M. Ćurković Perica1 1 Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) 2 Department of Forest Genetics, Dendrology and Botany, Faculty of Forestry, University of Zagreb, Zagreb, Croatia th
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Cryphonectria parasitica is a pathogenic fungus that causes chestnut blight. Diseased chestnuts recover naturally if the fungus which caused the canker is infected with Cryphonectria hypovirus 1 (CHV1). This virus causes the conversion of fungus from virulent into hypovirulent and thus enables biological control of the disease. CHV1 is dsRNA virus without a capsid. The presence of this dsRNA in the mycelium reduces virulence and reproductive capacities of the fungus. If CHV1 is introduced into an active canker caused by virulent C. parasitica strain, canker expansion ceases and callus (healing canker) is formed. Therefore, it is important that hypovirulent strains persist in C. parasitica populations. The aim of this research was to evaluate the diversity of C. parasitica populations in healed cankers in Ozalj and Markuševac in Zagreb. Bark samples were collected from 24 heal ed cankers; from callus tissue and callus‐ wood interface pure C. parasitica cultures were isolated. The presence of CHV1 was confirmed by dsRNA isolation and RT‐PCR. It is interesting that virulent C. parasitica strains were found quite frequently in healed cankers, although hypovirulent strains are usually associated with healing. We suspect that this is the result of either spontaneous loss of the virus from hypovirulent fungal strain, which previously caused healing or colonization of the callus by new, virulent, fungal strains which could reinitiate the disease on the healed cankers. Key words: Cryphonectria parasitica, chestnut blight, Cryphonectria hypovirus 1 (CHV1), healed canker O‐118 OPSTOJNOST VIRUSA Cryphonectria hypovirus 1 MOŽE OVISITI O NJEGOVOM PRIJENOSU IZMEĐU RAZLIČITIH TIPOVA VEGETATIVNE KOMPATIBILNOSTI GLJIVE Cryphonectria parasitica L. Nuskern1, Z. Katanić2, Lj. Krstin2, M. Ježić1, M. Ćurković‐Perica1 1 Zavod za mikrobiologiju, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska (mirna.curkovic‐[email protected]) 2 Odjel za Biologiju, Sveučilište Josipa Jurja Strossmayera, Osijek, Hrvatska Cryphonectria parasitica, fitopatogena gljiva koja uzrokuje rak kestenove kore značajno je uništila sastojine pitomoga kestena u Europi. Gljiva je domaćin virusa Cryphonectria hypovirus 1 koji omogućuje prirodnu biokontrolu ovog patogena. Učestali način prijenosa virusa između različitih sojeva gljive je putem anastomoze hifa. Mogućnost anastomoze regulirana je sustavom vegetativne kompatibilnosti: prijenos virusa najuspješniji je između micelija istog vegetativnog (vc) tipa, dok je između micelija različitih vc tipova otežan. U laboratorijskim uvjetima prenijeli smo različite sojeve virusa u izolate gljive različitih vc tipova. Zamijetili smo da je opstojnost virusa u miceliju akceptoru ovisila o tome pripadaju li donorski i akceptorski micelij istom ili različitom vc tipu. Pretpostavljamo da do gubitka virusa iz micelija dolazi zbog nedovoljne prilagodbe na novog domaćina koji je drugačijeg vc tipa. Rezultati dobiveni in vitro ne moraju nužno implicirati isti proces u prirodi, ali čini se da se ovi rezultati podudaraju s rezultatima terenskih istraživanja u kojima je zamijećeno da u kalusima, rak‐ranama zaraslima pod utjecajem gljive zaražene virusom, često nakon nekog vremena više ne nalazimo virus. Ključne riječi: biološka kontrola, invazivna vrsta, virulentnost PERSISTENCE OF Cryphonectria hypovirus 1 IN Cryphonectria parasitica MIGHT BE AFFECTED BY THE TRANSFER BETWEEN THE DIFFERENT VEGETATIVE COMPATIBILITY TYPES OF THE HOST L. Nuskern1, Z. Katanić2, Lj. Krstin2, M. Ježić1, M. Ćurković‐Perica1 th
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1
Division of Microbiology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia (mirna.curkovic‐[email protected]) 2 Department of Biology, Josip Juraj Strossmayer University, Ulica cara Hadrijana 8A, HR‐31000 Osijek, Croatia Phytopathogenic fungus Cryphonectria parasitica, causing agent of chestnut blight, severely devastated sweet chestnut forests in Europe. This fungus is a host of the virus Cryphonectria hypovirus 1, which is a natural biocontrol agent of this pathogen. Hyphal anastomosis is a frequent way of virus transmission between fungal strains. The persistence of anastomosis is regulated by vegetative compatibility (vc) system of the fungus. The most efficient virus transmission occurs between mycelia of the same vc type, but it becomes difficult between mycelia that belong to different vc types. In laboratory conditions, we transferred various virus strains into various fungal isolates belonging to different vc types. The persistence of the virus in the acceptor mycelia was determined by vc types of both, the virus‐donor and virus‐acceptor mycelia in cases when they belonged to different vc types. We hypothesize that the loss of the virus is caused by unsuccessful adaptation of the virus to the new host of different vc type. These in vitro results do not necessarily implicate similar scenario in the natural conditions, but they support observation in nature, where it was found that calli (healed cankers formed under the influence of hypovirulent fungus) often lose viruses with time. Key words: biological control, invasive species, virulence
Komparativna fiziologija, imunobiologija i biologija čovjeka Comparative physiology, immunobiology and human biology IO‐5 PREDVIĐANJE ISHODIŠNIH STANICA RAKA NA TEMELJU ORGANIZACIJE KROMATINA R. Karlić1*, P. Polak2,3*, A. Koren3,4, R. Thurman5, R. Sandstrom5, M. S. Lawrence3, A. Reynolds5, E. Rynes5, K. Vlahoviček1,7, J. A. Stamatoyannopoulos5,6, S. R. Sunyaev2,3 * ( podjednako pridonijeli radu) 1 Grupa za bioinformatiku, Zavod za molekularnu biologiju, Biološki odsjek, Prirodoslovno matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) 2 Division of Genetics, Department of Medicine, Brigham & Women’s Hospital and Harvard Medical School, Boston, Massachusetts, SAD 3 The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, SAD 4 Department of Genetics, Harvard Medical School, Boston, Massachusetts, SAD 5 Department of Genome Sciences, University of Washington, Seattle, Washington, SAD 6 Department of Medicine, Division of Oncology, University of Washington, Seattle, Washington SAD 7 Department of Informatics, University of Oslo, Oslo, Norveška Rak je bolest potencirana somatskim mutacijama, koje nisu jednoliko distribuirane duž genoma stanice raka, već se pojedine genomske regije razlikuju u lokalnoj gustoći somatskih mutacija. Pokazano je da je organizacija kromatina jedna od glavnih odrednica genomske raspodjele somatskih mutacija u stanicama raka. Kako su i somatske mutacije i organizacija kromatina tkivno specifične pojave, koristeći metode računalne biologije usporedili smo raspodjelu mutacija u više neovisnih tipova raka s brojnim epigenetskim podacima mjerenim u različitim th
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normalnim tkivima. Pokazali smo da epigenetska svojstva i vrijeme replikacije objašnjavaju do 86% varijance u raspodjeli gustoće mutacija duž genoma različitih tipova raka. Najbolja točnost predviđanja lokalne gustoće somatskih mutacija dobivena je upotrebom epigenetskih svojstava stanica u kojima je proučavani tip raka najvjerojatnije nastao. Uz to, otkrili smo da je moguće točnije predvidjeti raspodjelu mutacija u raku na temelju kromatinske strukture stanica u kojima je rak nastao nego na temelju kromatinske strukture odgovarajućih staničnih linija. Nadalje, pokazali smo da se u skoro 90% slučajeva tip stanica iz kojih je rak nastao može precizno odrediti na temelju raspodjele mutacija uz njegovu genomu. Dakle, naši rezultati pokazuju da genom stanica raka obuhvaća mnoštvo informacija o identitetu i epigenetskim obilježjima stanica u kojima je pojedini rak nastao. Taj podatak, pak, može biti izuzetno važan u dijagnozi i tretmanu malignih bolesti. Ključne riječi: epigenetska obilježja, genom, rak, somatske mutacije USING CHROMATIN ORGANIZATION TO PREDICT CANCER CELL‐OF‐ORIGIN R. Karlić1*, P. Polak2,3*, A. Koren3,4, R. Thurman5, R. Sandstrom5, M. S. Lawrence3, A. Reynolds5, E. Rynes5, K. Vlahoviček1,7, J. A. Stamatoyannopoulos5,6, S. R. Sunyaev2,3 (*equally contributed) 1 Bioinformatics Group, Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia 2 Division of Genetics, Department of Medicine, Brigham & Women’s Hospital and Harvard Medical School, Boston, Massachusetts, USA 3 The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA 4 Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA 5 Department of Genome Sciences, University of Washington, Seattle, Washington, USA 6 Department of Medicine, Division of Oncology, University of Washington, Seattle, Washington, USA 7 Department of Informatics, University of Oslo, Oslo, Norway Cancer is a disease potentiated by mutations in somatic cells, which are not distributed uniformly along the human genome. Instead, different human genomic regions vary by up to fivefold in the local density of cancer somatic mutations. Epigenomic organization has been proposed as a major determinant of the cancer mutational landscape. However, both somatic mutagenesis and epigenomic features are highly cell‐type‐specific. We investigated the distribution of mutations in multiple independent samples of diverse cancer types and compared them to cell‐type‐specific epigenomic features. Here we show that chromatin accessibility and modification, together with replication timing, explain up to 86% of the variance in mutation rates along cancer genomes. The best predictors of local somatic mutation density are epigenomic features derived from the most likely cell type of origin of the corresponding malignancy. Moreover, we find that cell‐of‐origin chromatin features are much stronger determinants of cancer mutation profiles than chromatin features of matched cancer cell lines. Furthermore, we show that the cell type of origin of a cancer can be accurately determined based on the distribution of mutations along its genome. Thus, the DNA sequence of a cancer genome encompasses a wealth of information about the identity and epigenomic features of its cell of origin. This information can be important for the accurate diagnosis and treatment of malignant diseases. Key words: cancer, epigenomic features, genome, somatic mutation th
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O‐119 REPLIKACIJA U B‐STANIČNIM LIMFOMIMA V. Tadić, P. Bašić Palković, B. Sasi, M. Klasić, P. Korać Zavod za molekularnu biologiju, Biološki odsjek, Prirodoslovno‐matemtički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) Mutacije pojedinih gena smatraju se ključnim koracima tumorigeneze, no to je složen proces koji uključuje i regulaciju transkripcije i adaptaciju tumorskih stanica na signale iz mikrookoliša. Iako tumorske stanice pojedinih vrsta B‐limfoma potječu od B‐stanica istog stadija diferencijacije, njihova prilagodba na mikrookoliš dovodi do promjene ravnotežnog stanja proteina uključenih u proces replikacije. Replikacija tako postaje manje pouzdana pa dolazi do nakupljanja dodatnih pogrešaka koje uzrokuju bržu progresiju tumora. U ovome je istraživanju analizirana količina transkripata onih gena koji kodiraju ključne proteine prereplikacijskog/replikacijskog kompleksa (PCNA, MCM2, MCM7, GMNN i CDT1) te proteine koji osiguravaju ispravnu uspostavu kromatina nakon prepisivanja DNA (p300, DNMT1 i EZH2). Istraživanje je provedeno na uzorcima B‐limfoma različitih stupnjeva agresivnosti. Iz tumorskog tkiva laserskom mikrodisekcijom izdvojene su tumorske stanice i okolno netumorsko tkivo, a razina ekspresije navedenih gena određena je lančanom reakcijom polimerazom u stvarnom vremenu. Kao kontrola služile su tonzile. Rezultati su pokazali značajno povećanu ekspresiju gena DNMT1, MCM2, CDT1 i p300 te smanjenju ekspresiju geminina u tumorima veće agresivnosti. Ova studija ukazuje na važnost promjene ekspresije gena uključenih u replikaciju i ponovnu uspostavu kromatina za progresiju malignih tumora. Ključne riječi: B‐stanični limfomi, replikacija, kromatin REPLICATION IN B‐CELL LYMPHOMA V. Tadić, P. Bašić Palković, B. Sasi, M. Klasić, P. Korać Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) Mutations of specific genes are considered as key steps in tumorigenesis, but this process equally includes transcription regulation and tumor cell adaptation to the signals from microenvironment. Although tumor cells of specific B‐cell lymphoma have origin in B‐cells from the same differentiation stage, their adaptation to the microenvironment changes equilibrium of proteins involved in replication process and makes them different from each other. Replication then becomes less accurate leading to the accumulation of aberrations in DNA. Additional changes contribute to tumor progression. In this study we analyzed expression level of prereplication/replication complex proteins (PCNA, MCM2, MCM7, GMNN and CDT1) and proteins included in reassembly of chromatin after the replication (p300, DNMT1 and EZH2). Study was conducted on tissue samples with the diagnosis of diffe rent B‐lymphomas. From the tissue samples, using laser microdissection technique, tumor cells and non‐tumor parts were isolated. Expression levels were analyzed using real‐time quantitative PCR. As a control, tonsils were used. Results showed significant DNMT1, MCM2, CDT1 and p300 overexpression as well as geminin underexpression during B‐lymphoma progression. This study suggests importance of changes in expression levels of genes involved in replication and chromatin reassembly for malignant tumor progression. Key words: B‐cell lymphoma, replication, chromatin th
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O‐120 RAZVOJ TESTOVA ZA ANALIZU METILACIJE DNA REGULATORNIH REGIJA GENA GMNN I EZH2 I NJIHOVA PRIMJENA U ISTRAŽIVANJU PODTIPOVA DIFUZNOG B‐VELIKOSTANIČNOGA LIMFOMA V. Vuković*, M. Acman*, M. Klasić, P. Dobrinić, P. Korać (*podjednako pridonijeli radu) Zavod za molekularnu biologiju, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) Istraživanja malignih tumora ukazala su na važnost gena uključenih u replikaciju i ponovnu uspostavu kromatina u onkogenezi. Njihova poremećena funkcija može biti povezana s epigenetičkim promjenama. Cilj ovog istraživanja bio je analizirati stupanj metilacije DNA regulatornih regija gena GMNN i EZH2 kod podtipova difuznog B‐velikostaničnog limfoma. U tu su svrhu razvijeni testovi za procjenu stupnja metilacije pojedinih CpG mjesta unutar regulatornih regija tih gena. Odabrani su parovi početnica koji lančanom reakcijom polimerazom umnažaju fragmente bisulfitno konvertirane DNA unutar regulatornih regija gena od interesa. Razvijena su tri testa za regulatorne regije gena GMNN i četiri testa za regulatorne regije gena EZH2. Nakon toga određeni su optimalni uvjeti reakcija pirosekvenciranja. Završno je razvijen po jedan test za svaki gen. Ti su testovi primijenjeni u analizi podtipova difuznog B‐ velikostaničnog limfoma. Analizirani dijelovi regulatornih regija gena GMNN i EZH2 nisu pokazali značajne razlike između podtipova. Budući da su ovim istraživanjem obuhvaćeni relativno mali dijelovi regulatornih regija oba gena, analizu bi trebalo proširiti na dodatna CpG mjesta te uključiti veći broj uzoraka. Ključne riječi: GMNN, EZH2, DLBCL, pirosekvenciranje, metilacija DNA ASSAY DEVELOPMENT FOR DNA METHYLATION ANALYSIS OF REGULATORY REGIONS OF GENES GMNN AND EZH2 AND THEIR APPLICATION IN EVALUATION OF DIFFUSE LARGE B‐CELL LYMPHOMA SUBTYPES V. Vuković*, M. Acman*, M. Klasić, P. Dobrinić, P. Korać (*equally contributed) Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) Cancer research pointed to the importance of genes involved in replication and reestablishment of chromatin in oncogenesis. Gene dysfunction in tumours is often associated with epigenetic changes. The aim of this study was to analyse DNA methylation of regulatory regions of genes GMNN and EZH2 in diffuse large B‐cell lymphoma subtypes. Methylation assays were developed for the analysis of those regions at single CpG site resolution. Primers for PCR‐amplification of bisulfite treated DNA fragments were designed. PCR conditions were optimized for the amplification of three and four fragments of regulatory region of GMNN and EZH2, respectively. Subsequently, optimal conditions for pyrosequencing were determined. Finally, one assay per gene was created. Newly developed assays were used in the analysis of diffuse large B‐cell lymphoma subtypes. The results showed no statistically significant difference between the subtypes. Since the analysis covered only a small part of the regulatory regions of GMNN and EZH2, the study should be expanded to additional CpG sites and repeated on a larger group of patients. Key words: GMNN, EZH2, DLBCL, pyrosequencing, DNA methylation th
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O‐121 HISTOKOMPATIBILNOST I IMUNOGENETIKA U TRANSPLANTACIJI SOLIDNIH ORGANA U HRVATSKOJ R. Žunec Zavod za tipizaciju tkiva, KBC Zagreb, Zagreb, Hrvatska ([email protected]‐zagreb.hr) Program transplantacije solidnih organa odvija se u Hrvatskoj od 2007. g. u okviru međunarodne organizacije Eurotransplant. Pristupanje je uz ispunjavanje mnogobrojnih zakonskih i organizacijskih uvjeta, zahtijevalo i posjedovanje laboratorija za tipizaciju tkiva s važećom akreditacijom Europske federacije za imunogenetiku (EFI) kako bi se osigurala standardizirana kvaliteta rada na području histokompatibilnosti i imunogenetike. Ovaj preduvjet Hrvatska je ispunila akreditiranjem Zavoda za tipizaciju tkiva KBC Zagreb (ZZTT) 2007. g., koji je time postao prvi medicinski laboratorij u Hrvatskoj s europskom akreditacijom. U ZZTT danas se provodi imunogenetska obrada bolesnika za prijavu na listu čekanja za 4 transplantacijska centra (KBC Zagreb, KB Merkur, KB Dubrava i KBC Osijek) te imunogenetska obrada kadaveričnih davatelja organa 15 donorskih bolnica. U razdoblju 2007.‐2015. godine imunogenetska obrada za listu čekanja napravljena je za 1842 primatelja bubrega, 364 primatelja srca, 1074 primatelja jetre i 92 primatelja gušterače. U istom razdoblju provedeni su imunogenetski testovi za 1423 transplantacije bubrega, 230 transplantacija srca, 774 transplantacija jetre i 75 transplantacija gušterače. Ovako intenzivan program učinio je Hrvatsku jednom od vodećih zemalja u svijetu na području transplantacije organa, čemu Zavod za tipizaciju tkiva KBC Zagreb daje svoj stručan i znanstveni doprinos kao europski akreditiran imunogenetski centar izvrsnosti. Ključne riječi: imunogenetika, tipizacija tkiva, transplantacija, solidni organi HISTOCOMPATIBILITY AND IMMUNOGENETICS IN SOLID ORGAN TRANSPLANTATION IN CROATIA R. Žunec Tissue Typing Center, UHC Zagreb, Zagreb, Croatia ([email protected]‐zagreb.hr) Transplantation program of solid organs in Croatia is the integral part of Eurotransplant since 2007, when Croatia become a full member of this biggest European non‐profit organization for optimal use and cross‐border sharing of cadaveric donor organs. Joining Eurotransplant requests fulfilling numerous organizational and legislative prerequisites having an EFI accredited tissue typing laboratory being the one of them. Croatia fulfilled these criteria when Tissue Typing Centre UHC Zagreb obtained the EFI accreditation in 2007, becoming the first Croatian medical laboratory with European accreditation. Since then, Tissue Typing Center Zagreb is responsible for immunogenetic testing of patients from 4 transplantation centers (UHC Zagreb, CH Merkur, CH Dubrava and UHC Osijek) and for donor typing from 15 donor hospitals. In the period 2007‐2015 immunogenetic testing were conducted for 1842 kidney recipients, 364 heart recipients, 1074 liver recipients and 92 pancreas recipients. In the same period, testings were done for supporting 1423 kidney transplantations, 230 hear transplantation, 774 liver transplantations and 75 pancreas transplantations. Such an intensive donor and transplantation program put Croatia among the world most successful countries with Tissue Typing Center giving its professional and scientific impact as a European accredited immunogenetic center of excellence. Key words: immunogenetics, tissue typing, transplantation, solid organs th
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O‐122 HISTOKOMPATIBILNOST I IMUNOGENETIKA U ALOGENOJ TRANSPLANTACIJI KRVOTVORNIH MATIČNIH STANICA U HRVATSKOJ Z. Grubić Zavod za tipizaciju tkiva, KBC Zagreb, Zagreb, Hrvatska ([email protected]‐zagreb.hr) Program transplantacije krvotvornih matičnih stanica (TKMS) započeo je u Hrvatskoj 1983. godine prvom alogenom transplantacijom od srodnog davatelja. Odabir davatelja prema imunogenetskim kriterijima osnova je i od presudnog značaja za uspješan ishod TKMS, a provodi se u Zavodu za tipizaciju tkiva KBC Zagreb. U programu srodne TKMS prosječno se godišnje obradi 150 bolesnika i 700 članova obitelji, s uspješnošću pronalaska HLA genotipski identičnog davatelja u oko 30% obitelji. Bolesnici kojima davatelj nije pronađen u obitelji, uključuju se u program TKMS s HLA fenotipski podudarnog nesrodnog davatelja. Program se temelji na postojanju nacionalnih registara uključenih u svjetski registar (Bone Marrow Donors Worldwide ‐ BMDW) dobrovoljnih davatelja KMS. Hrvatski registar dobrovoljnih davatelja KMS (RDDKMS) danas ima upisanih, i u Zavodu za tipizaciju tkiva imunogenetski obrađenih, više od 40 000 dobrovoljnih davatelja, dok BMDW broji 26 milijuna davatelja. Odabir nesrodnog davatelja uključuje složene algoritme pretraživanja hrvatskog i svjetskog registra, tipizaciju visokog razlučivanja lokusa HLA (A, B, C, DRB1 i DQB1) bolesnika i odabranih mogućih davatelja te završni odabir s potvrdnim testiranjem primatelja i davatelja. Godišnje se ovaj postupak provodi za oko 100 bolesnika, a uključuje obradu i 400 mogućih nesrodnih davatelja. TKMS je u Hrvatskoj opsežan i uspješan program liječenja u kojem Zavod za tipizaciju tkiva KBC Zagreb ima značajnu i nezaobilaznu ulogu. Ključne riječi: imunogenetika, tipizacija tkiva, transplantacija, krvotvorne matične stanice HISTOCOMPATIBILITY AND IMMUNOGENETICS IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION IN CROATIA Z. Grubić Tissue Typing Center, UHC Zagreb, Zagreb, Croatia ([email protected]‐zagreb.hr) Hematopoietic stem cell transplantation (HSCT) program in Croatia started in 1983 when first allogeneic transplantation from related donor was performed. Recruitment of the donor following immunogenetic criteria is a crucial step in HSCT which is done exclusively in Tissue Typing Center UHC Zagreb. Related HSCT program includes testings of around 150 patients and 700 family members annually, resulting in successful search of HLA genotipically identical donor in 30% of families. Patients for whom the donor was not found within family are included in HSCT program from HLA phenotipically identical unrelated donor. This program relies on national registries of unrelated volunteer HSCT donors which are all included in the world registry Bone marrow donors worldwide (BMDW). Croatian bone marrow donor registry (CBMDR) has more than 40 000 registered donors, all of them being HLA typed in Tissue Typing Center Zagreb, while BMDW reached the number of 26 million donors. Protocols for finding unrelated donor includes sophisticated search algorithms of CBMDR and BMDW, high resolution HLA typing at five loci (A, B, C, DRB1, DQB1) and final confirmatory testings of the recipient and the most phenotipically compatible donor. Around 100 patients are included in this program annually together with more than 400 potential unrelated donors. HSCT is a large and successful program in Croatia with a Tissue Typing Center UHC Zagreb being its integral part with a crucial role. th
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Key words: immunogenetics, tissue typing, transplantation, hematopoietic stem cells O‐123 AKUTNI UTJECAJ KOMBINIRANOG TRETMANA DIJAFRAGMALNOG DISANJA I PROGRESIVNE MIŠIĆNE RELAKSACIJE NA ANTIOKSIDACIJSKU UČINKOVITOST SLINE U MLADIH ODRASLIH LJUDI POVEZAN S NJIHOVIM CRTAMA OSOBNOSTI H. Lepeduš1, A. Kurtović1, I. Jozić2, D. Gašo Sokač3, I. Marčinko1, V. Cesar4 1 Odsjek za psihologiju, Filozofski fakultet, Sveučilište J. J. Strossmayera u Osijeku, Osijek, Hrvatska ([email protected]) 2 Sprećanska 31, Osijek, Hrvatska 3 Prehrambeno‐tehnološki fakultet, Sveučilište J. J. Strossmayera u Osijeku, Osijek, Hrvatska 4 Odjel za biologiju, Sveučilište J. J. Strossmayera u Osijeku, Osijek, Hrvatska Stres je postao sastavni dio modernog stila života današnjice, te sukladno tome i predmet znanstvenih istraživanja u različitim disciplinama. Brojni dokazi biokemijskih mehanizama patogeneze velikog broja bolesti i poremećaja povezanih sa stresom pokazali su poveznicu sa stvaranjem i djelovanjem reaktivnih kisikovih jedinki (ROS). Kad su prisutne u suvišku, ROS uzrokuju oksidacijska oštećenja najvažnijih biomolekula te na taj način dovode do poremećaja u funkcioniranju stanica. Veliki broj molekula pokazuje antioksidacijska svojstva što omogućuje učinkovitu zaštitu od oksidacijskog stresa. U ovom istraživanju određena je antioksidacijska učinkovitost sline (SAC) mladih odraslih ljudi prije i neposredno nakon jednog kombiniranog tretmana (u trajanju od oko 35 min.) koji se sastojao od dijafragmalnog disanja (DB) i progresivne mišićne relaksacije (PMR). I DB i PMR su ne‐medikamentozni tretmani koji se rutinski koriste u kliničkoj psihologiji i psihijatriji. Primjenom jednog tretmana došlo je do značajnog povećanja vrijednosti SAC. Individualne razlike u porastu SAC su, međutim, bile u rasponu od 2‐3% do 60%. Pokazalo sa da je takav veliki rasap vrijednosti u značajnoj negativnoj korelaciji s neuroticizmom, jednom od 'velikih pet' crta osobnosti. Biološki učinci koji su se pokazali u ovom istraživanju potvrđuju potrebu upotrebe DB i PMR kao vrlo moćnih tehnika ne samo u kliničkoj praksi već i u smislu svakodnevne mentalne higijene kod zdrave populacije mladih odraslih ljudi. ACUTE RESPONSES OF SALIVA ANTIOXIDANT CAPACITY TO COMBINED DIAPHRAGMATIC BREATHING AND PROGRESSIVE MUSCLE RELAXATION TREATMENTS IN YOUNG ADULTS RELATED TO THEIR PERSONALITY TRAITS H. Lepeduš1, A. Kurtović1, I. Jozić2, D. Gašo Sokač3, I. Marčinko1, V. Cesar4 1 Department of Psychology, Faculty of Humanities and Social Sciences, University of J. J. Strossmayer in Osijek, Osijek, Croatia ([email protected]) 2 Sprećanska 31, Osijek, Croatia 3 Faculty of Food Technology, University J. J. Strossmayer in Osijek, Osijek, Croatia 4 Department of Biology, University of J. J. Strossmayer in Osijek, Osijek, Croatia Presently, stress became integral part of the modern life style and thus a scientific topic in different disciplines. Biochemical mechanisms of stress‐related pathogenesis are well documented in a broad spectrum of illnesses and disorders and were shown to comprise the formation and action of different reactive oxygen species (ROS). When present in excess, ROS induce oxidative damage to essential biomolecules and in turn disturb normal cell functioning. A plethora of molecules reveals antioxidative properties facilitating efficient th
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protection against oxidative stress. In this investigation saliva antioxidant capacity (SAC) was measured in young adults before and immediately after a single combined treatment (duration of app. 35 min.) consisting of diaphragmatic breathing (DB) and progressive muscle relaxation (PMR). Both, DB and PMR are routinely used as non medicamentous treatments in clinical psychology and psychiatry. After a single treatment applied the significant increase in SAC was revealed. However, the individual differences in SAC increase appeared to be dissipated from 2‐3 to 60%. Such great dissipation was further confirmed to be significantly negatively correlated with the neuroticism, the one of the 'big five' personality traits. Biological effects revealed in our study confirmed the requirement of DB and PMR application as powerful anti‐stress tools in both clinical praxis and especially as daily mental hygiene in healthy young adult population. Key words: stress, saliva, diaphragmatic breathing, progressive muscle relaxation, non medicamentous anti‐stress treatments O‐124 MONKEYS WITH PERSONALITY: CONSISTENT INTER‐INDIVIDUAL DIFFERENCES IN COMMON MARMOSETS (Callithrix jacchus) V. Šlipogor1,2, T. Gunhold‐de Oliveira1, J.J.M. Massen1, Z. Tadić2, T. Bugnyar1 1 Department of Cognitive Biology, University of Vienna, Vienna, Austria ([email protected]) 2 Division of Animal Physiology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia The first ideas of personality came from human psychology, nowadays is this research question studied in a wide range of study species, from insects to non‐human primates. Consistent individual differences (animal personalities) are defined as groups of correlated behaviours, that are temporally and contextually repeatable within an individual, and are usually divided in three non‐social ('Boldness‐Shyness', 'Exploration‐Avoidance' and 'Activity') and two social ('Aggressiveness' and 'Sociability') behavioural traits. In this study, we tested 21 individuals from three family groups of common marmosets (Callithrix jacchus) in five different experiments (general activity, novel food, novel object, predator and foraging under risk) in a solitary setting. Our results indicated that monkeys behaved consistently not only throughout time, but also across different contexts. These behaviours formed a construct of four major non‐social personality components: 'Boldness‐Shyness in Foraging', 'Boldness‐Shyness in Predation', 'Stress‐Activity' and 'Exploration‐Avoidance'. The models revealed no sex or age differences in the components, but they revealed a difference between groups in 'Exploration‐Avoidance'. As common marmosets are highly gregarious animals, we will discuss the presence of the group‐ specific personality trait 'Exploration‐Avoidance' with regards to the possible influence of social environment on behaviour of individuals, even outside the immediate social context. Key words: personality, consistent inter‐individual differences, exploration‐avoidance, common marmosets th
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O‐125 USPOREDBA MASNOKISELINSKOG SASTAVA POTKOŽNOG MASNOG TKIVA SMEĐEG MEDVJEDA (Ursus arctos) I SIVOG VUKA (Canis lupus) L. Vranković1, I. Delaš2, S. Reljić3, Đ. Huber3, J. Kusak3, K. Klobučar4, G. Krivić4, D. Octenjak4, N. Mikinac4, Z. Stojević1, J. Aladrović1 1 Zavod za fiziologiju i radiobiologiju Veterinarskog fakulteta Sveučilišta u Zagrebu, Zagreb, Hrvatska ([email protected], [email protected], [email protected]) 2 Zavod za kemiju i biokemiju Medicinskog fakulteta Sveučilišta u Zagrebu, Zagreb, Hrvatska 3 Zavod za biologiju Veterinarskog fakulteta Sveučilišta u Zagrebu, Zagreb, Hrvatska 4 Studenti Veterinarskog fakulteta Sveučilišta u Zagrebu, Zagreb, Hrvatska Analiza masnokiselinskog sastava (MS) masnog tkiva koristi se za utvrđivanje prehrambenih navika slobodno živućih životinja. Ovisno o vrsti, mnoge masne kiseline (MK) masnog tkiva potječu direktno iz hrane. Cilj ovog rada bio je bolje razumijevanje prehrambenih navika i fiziološkog statusa slobodno živućih jedinki smeđeg medvjeda (SM) i sivog vuka (SV), što je ključno za strategije gospodarenja i zaštite populacija divljih životinja. Istraživanje je provedeno na 91 jedinki SM (ženke: n=25, 4,66±2,65 g.; mužjaci: n=66, 5,30±2,72 g.) i 20 jedinki SV (ženke: n=11, 1,95±1,86 g.; mužjaci: n=9, 2,59±1,59 g.). Uzorci su skupljeni tijekom sezone lova (uzorci SM 2013. i uzorci SV 2009.‐2011.). Nakon odmrzavanja uzoraka, homogenizacije i ekstrakcije lipida, MS određen je plinskom kromatografijom. U MS potkožnog masnog tkiva obje vrste dominiraju MK s jednom dvostrukom vezom (48% u ženki i 41% u mužjaka SM vs. 46% u ženki i 46% u mužjaka SV). Zasićene MK čine 26% u ženki i 38% u mužjaka SM vs. 42% u ženki i 44% u mužjaka SV. Višestrukonezasićene MK (PUFA) čine 26% u ženki i 21% u mužjaka SM. U ženki SV PUFA su bile zastupljene u 12% dok su u mužjaka činile 10%. U MS potkožnog masnog tkiva SM i SV utvrđene su značajne razlike s obzirom na dob, tjelesnu masu, sezonu i lokaciju uzorkovanja. Sličnosti MS potkožnog masnog tkiva u SM i SV proistječu iz filogeneze zvijeri, dok su razlike rezultat različitog fiziološkog statusa i prehrambenih navika jedinki. Ključne riječi: smeđi medvjed, sivi vuk, masnokiselinski sastav, potkožno masno tkivo COMPARISON OF FATTY ACID COMPOSITION OF SUBCUTANEOUS ADIPOSE TISSUE OF BROWN BEAR (Ursus arctos) AND GRAY WOLF (Canis lupus) L. Vranković1, I. Delaš2, S. Reljić3, Đ. Huber3, J. Kusak3, K. Klobučar4, G. Krivić4, D. Octenjak4, N. Mikinac4, Z. Stojević1, J. Aladrović1 1 Department for Physiology and Radiobiology, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia ([email protected]) 2 Department for Chemistry and Biochemistry, School of Medicine University of Zagreb, Zagreb, Croatia 3 Department for Biology, Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia 4 Students of Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia Analysis of fatty acid (FA) composition is a technique used to examine the foraging habits of free‐ ranging animals. Depending on the species, many of the FAs contained in adipose tissue are derived directly from diet. The objective of this study was better understanding of foraging patterns and physiological status of free ranging brown bears (BB) and gray wolves (GW), which is essential for management and conservation strategies of wildlife populations. The study was conducted on 91 BB (female: n=25, 4.66±2.65 yr.; male: n=66, 5.30±2.72 yr.) and 20 GW (female: n=11, 1.95±1.86 yr.; male: n=9, 2.59±1.59 yr.). Samples were collected during the legal hunting th
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season (2013 for BB and 2009‐2011 for GW). After thawing, homogenization and lipid extraction, the FA composition was determined by gas chromatography. The subcutaneous adipose tissue (SAT) of both species is do minated by FAs with one double bond (48% in female and 41% in male BB vs. 46% in female and 46% in male GW). Saturated fatty acids constituted of 26% in female and 38% in male BB vs. 42% in female and 44% in male GW. Polyunsaturated fatty acids were found in 26% in female and 21% in male BB. In females GW PUFA was in 12% while in males it was 10%. The FAs of SAT of BB and GW showed age‐, body mass‐, season‐ and location related significant differences. Similarities in FAs of BB and GW are derived from phylogeny of carnivores, while diferences are the result of different physiological status and foraging patterns. Key words: brown bear, gray wolf, fatty acid composition, subcutaneous adipose tissue
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POSTERSKE PREZENTACIJE POSTERS
KONZERVACIJSKA BIOLOGIJA, ZAŠTITA PRIRODE I OKOLIŠA CONSERVATIONAL BIOLOGY, NATURE AND ENVIRONMENT PROTECTION P‐1 KOCKAVICA (Fritillaria meleagris L., LILIACEAE) – 10 GODINA AKCIJE PRAĆENJA STANJA ZA OSNOVNE I SREDNJE ŠKOLE I. Boršić, V. Posavec Vukelić, T. Kirin Odjel za divlje i udomaćene svojte i staništa, Državni zavod za zaštitu prirode, Zagreb, Hrvatska ([email protected]) Kockavica (Fritillaria meleagris L., Liliaceae) je proljetnica vlažnih livada i svijetlih, otvorenih vlažnih šikara i šuma. Rasprostranjena je u kontinentalnoj Hrvatskoj, s nekoliko izoliranih nalazišta s područja Velebita. Prema Crvenoj knjizi vaskularne flore Hrvatske smatra se osjetljivom vrstom (VU). Akcija praćenja stanja kockavice započela je 2005. godine u suradnji sa školama Međimurske i Krapinsko‐zagorske županije. Do danas se akcija proširila, te se provodi u suradnji s javnim ustanovama za upravljanje zaštićenim prirodnih vrijednosti ukupno devet županija, Međimurske, Krapinsko‐zagorske, Koprivničko‐križevačke, Varaždinske, Bjelovarsko‐bilogorske, Sisačko‐moslavačke, Zagrebačke, Karlovačke i Požeško‐slavonske županije, te osnovnim i srednjim školama ovih županija. Akcijom su prikupljeni značajni podaci o rasprostranjenosti kockavice u Hrvatskoj koji su prikazani u ovom priopćenju. Isto tako, akcijom je utvrđeno kako je kockavica s pojedinih nalazišta nestala, uglavnom zbog melioracija, širenja građevinskih područja, izgradnje cesta te pretvaranja livada u obradive površine. Temeljem svih dostupnih podataka, koji uključuju literaturne navode, vlastita terenska opažanja te podatke prikupljene akcijom praćenja stanja kockavice provedena je revizija kategorije ugroženosti kockavice u Hrvatskoj. Ključne riječi: prava kockavica, prebrojavanje, kategorija ugroženosti, prijetnje SNAKE'S HEAD FRITILLARY (Fritillaria meleagris L., LILIACEAE) – 10 YEARS OF MONITORING CAMPAIGN FOR PRIMARY AND SECONDARY SCHOOLS I. Boršić, V. Posavec Vukelić, T. Kirin Department for Wild and Domesticated Taxa and Habitats, State Institute for Nature Protection, Zagreb, Croatia ([email protected]) Snake's head fritillary (Fritillaria meleagris L., Liliaceae) is a spring flower of wet grasslands and light, open wet thickets and forests. It is widespread in continental Croatia, with a few isolated localities from Velebit area. According to the Red Book of Vascular Flora of Croatia it is considered a vulnerable species (VU). Snake's head fritillary monitoring campaign began in 2005 in cooperation with schools of Međimurje and Krapina‐Zagorje County. To date, the action spread, and is being conducted in collaboration with public institutions for management of protected natural values of the nine counties, Međimurje, Krapina‐Zagorje, Koprivnica‐ Križevci, Varaždin, Bjelovar‐Bilogora, Sisak‐Moslavina, Zagreb, Karlovac and Požega‐Slavonia County, and primary and secondary schools of these counties. During the action important data on the distribution of Snake's head fritillary in Croatia was collected, as presented in this presentation. Also, it was found that the Snake's head fritillary has disappeared from several localities, mainly due to land ameliorations, expansion of commercial or residential areas, road construction and the conversion of grassland to arable land. Based on all available data, th
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including the literature data, our own field observations and data collected through the monitoring campaign the threat category of Snake's head fritillary was reassessed. Key words: Fritillary, counting, threat category, threats P‐2 DEFINIRANJE EKOLOŠKIH CILJEVA U SKLADU S EKOLOŠKOM MREŽOM NATURA 2000 ZA POTREBE REVITALIZACIJE ERDUTSKO‐DALJSKOG DUNAVCA A. Đanić1, Z. Mesić2, M. Škunca1, A. Popijač1, E. Patčev1, S. Kapelj3, J. Križan4 1 Geonatura d.o.o. za stručne poslove zaštite prirode, Zagreb, Hrvatska ([email protected]) 2 Oikon d.o.o. ‐ Institut za primijenjenu ekologiju, Zagreb, Hrvatska 3 Udruga BIOM, Zagreb, Hrvatska 4 Gekom d.o.o., Hrvatska Područje Erdutsko‐daljskog dunavca je sustav od tri riječna rukavca u desnoj inundaciji Dunava. Dio je ekološke mreže Natura 2000 (područje očuvanja značajno za ptice 'HR1000016 Podunavlje i donje Podravlje', područje očuvanja značajno za vrste i staništa 'HR2000372 Dunav‐Vukovar'). Dosad izvedeni građevinski zahvati (željeznički i cestovni most, hidrotehnički objekti za potrebe održavanja plovnog puta), uz prirodno zapunjavanje riječnim materijalom, smanjenje dubine i eutrofikaciju, doprinose ubrzanju sukcesijskih procesa, zbog čega dolazi do promjene strukture i funkcije dunavca. Cilj projekta bio je izraditi idejno rješenje revitalizacije kojim bi se dugoročno osiguralo izmjenu i osvježavanje dunavca vodom iz Dunava, kako bi se postojeći nepovoljni procesi usporili te očuvalo ili poboljšalo stanje vodenih i močvarnih staništa, uz istovremeno osiguranje održivog korištenja područja. U sklopu projekta revitalizacije utvrđeno je postojeće stanje vodenih i močvarnih staništa te značaj područja za očuvanje faune, s naglaskom na ciljne vrste riba i ptica. Analizom stanja ciljnih vrsta i staništa te ključnih ekoloških uvjeta potrebnih za njihovo očuvanje, utvrđeni su specifični (ekološki) ciljevi. Svrha ekoloških ciljeva revitalizacije bila je dati smjernice i uvjete za projektiranje idejnog rješenja revitalizacije i planiranje korištenja prostora u svrhu rekreativnog ribolova i drugih turističkih aktivnosti. Ključne riječi: revitalizacija riječnih rukavaca, idejno rješenje, Natura 2000, očuvanje ciljnih vrsta i staništa DEFINING ECOLOGICAL OBJECTIVES ACCORDING TO ECOLOGICAL NETWORK NATURA 2000 FOR THE PURPOSE OF REVITALIZATION OF 'ERDUT‐DALJ DUNAVAC' A. Đanić1, Z. Mesić2, M. Škunca1, A. Popijač1, E. Patčev1, S. Kapelj3, J. Križan4 1 Geonatura Ltd. Consultancy in Nature Protection, Zagreb, Croatia ([email protected]) 2 Oikon Ltd. – Institute for Applied Ecology, Zagreb, Croatia 3 Association BIOM, Zagreb, Croatia 4 Gekom d.o.o., Zagreb, Croatia The area of 'Erdut‐Dalj dunavac' is the system of three river side arms in the right inundation of Danube River. It is part of ecological network Natura 2000 (SPA 'HR1000016 Podunavlje i donje Podravlje', SCI 'HR2000372 Dunav‐Vukovar'). Already implemented construction works (railway and road bridge, hydrotechnical objects for the maintenance of waterway), natural filling with river sediments, lowering of depth and eutrophication, all together contribute to th
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acceleration of succession processes, causing changes in structure and function of river side arms. The project aim was to design concept solution of revitalization to ensure long‐term water exchange and refreshment with water from Danube main channel. This would slow down the existent unfavourable processes in order to maintain or improve the state of aquatic and wetland habitats, and allow sustainable use of the area. Current state of aquatic and wetland habitats was assessed, as well as the value of the project area for conservation of fauna, with an emphasis on target fish and target bird species. Specific (ecological) objectives were defined based on the analysis of current status of target species and target habitats, and key ecological requirements needed for their conservation. The purpose of defining ecological objectives of revitalization is to give recommendations and framework for designing the concept solution and planning the use of the area for recreational fishing and other touristic activities. Key words: river side arms revitalization, concept solution, Natura 2000, conservation of target species and target habitats P‐3 SUŽIVOT LJUDI I PRIRODE NA PRIMJERU NACIONALNOG PARKA PLITVIČKA JEZERA – JE LI ISTO MOGUĆE? M. Gubić, M. Baričević, D. Fantina, A. Mojsović Ćuić Zdravstveno veleučilište, Zagreb, Hrvatska (ana.mojsovic‐[email protected]) Cilj provedenog istraživanja bio je ispitati kakve su mogućnosti suživota stanovništva zaštićenog područja s prirodom te jesu li u skladu sa zakonskom regulativom. U svrhu istraživanja provedeno je anketiranje među lokalnim stanovništvom. Na temelju rezultata ankete te direktnog uvida u način života lokalnog stanovništva u sklopu NP Plitvička jezera može se zaključiti da lokalno stanovništvo u najvećoj mjeri i dalje živi od turizma koji je vezan uz postojanje Nacionalnog parka, no ne u tolikoj mjeri kao nekad. Kao najveći problem života u ovom kraju stanovnici ističu da je školovanje mladih, u svrhu daljnjeg zapošljavanja u parku skoro u potpunosti nestalo, kako lokalne poljoprivrede povezane s opskrbom ugostiteljsko‐ turističkih sadržaja u parku više nema, a sezonske poslove rade ljudi iz svih krajeva Hrvatske, bez prednosti lokalnog stanovništva. Smatraju kako bi Uprava parka, kao i općine, zaštićenom području trebali pridati mnogo veću pozornost te bi samim time poboljšali suživot prirode i lokalnog stanovništva. Na samom kraju nameće se zaključak kako je suživot prirode i stanovništva moguć ukoliko se postigne ravnoteža između poštivanja zakonskih odredbi o zaštiti prirode te ukoliko iste ne onemogućuju radnje nužne za normalan život. Ključne riječi: Nacionalni park, Plitvička jezera, lokalno stanovništvo, suživot, priroda COEXISTENCE OF LOCAL POPULATION AND NATURE IN THE EXAMPLE OF THE PLITVICE LAKES NATIONAL PARK – IS IT POSSIBLE? M. Gubić, M. Baričević, D. Fantina, A. Mojsović Ćuić University of Applied Health Sciences, Zagreb, Croatia (ana.mojsovic‐[email protected]) The goal of research was to examine what are the possibilities of coexistence of the population in the protected area with the nature and whether they are in compliance with the statutory regulations. For the purpose of research, an opinion poll was conducted among the local th
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population. Based on the results of the poll and direct inspection of the manner of life of the locals within the Plitvice Lakes National Park, it may be concluded that the local population for the most part still lives of tourism, which is related to the National Park, but not in the proportions as it once did. As the major problem in their life, the locals point out that the schooling of the young for the purpose of further employment in the park has almost completely disappeared, that there is no longer local agriculture linked to the supply of the catering‐tourist facilities in the park, and the locals no longer have privileges in seasonal jobs which are nowadays also available to other people from all parts of Croatia. They think that the park's Management, as well as the municipalities, should devote much greater attention to the protected area, which would simultaneously improve coexistence of the nature and local population. At the end, the conclusion is that the coexistence of nature and locals is only possible if the balance is achieved between the compliance of statutory provisions on protection of nature and if such provisions do not hinder activities required for normal life. Key words: National Park, Plitvice Lakes, local population, coexistence, nature P‐4 ANALIZA METODA ZA PRAĆENJE PRIJELAZA DIVLJIH ŽIVOTINJA PREKO ZELENIH MOSTOVA G. Gužvica1, I. Bošnjak2, A. Bielen3, D. Babić4, B. Radanović‐Gužvica5, L. Šver3 1 Grupa za velike zvijeri, Geonatura d.o.o., Zagreb, Hrvatska ([email protected]) 2 Sveučilište u Zagrebu, Prirodoslovno‐matematički fakultet, Botanički zavod, Zagreb, Hrvatska 3 Sveučilište u Zagrebu, Prehrambeno‐biotehnološki fakultet, Zavod za biokemijsko inženjerstvo, Zagreb, Hrvatska 4 Byte Lab, Zagreb, Hrvatska 5 Hrvatski prirodoslovni muzej, Zagreb, Hrvatska Zeleni mostovi smanjuju negativan učinak prometnica na populacije divljih životinja, a njihova učinkovitost procjenjuje se različitim metodama praćenja. Temeljem trogodišnjeg praćenja četiri zelena mosta na autocesti A1, uspoređena je učinkovitost metode praćenja tragova na kontrolnoj pješčanoj traci, metode fotozamki i metode infracrvenih (IC) senzora. Za svaku metodu je utvrđeno koliko točno detektira različite vrste divljih životinja, kao i može li se temeljem nje procijeniti stvarni broj prijelaza. Granulometrijski sastav pješčane trake utjecao je na točnost određivanja životinjskih vrsta. Najbolji rezultati dobiveni su kada je korišten materijal s većim udjelom silta i gline. Što se tiče sastava vrsta, metodom praćenja tragova na kontrolnoj traci potcijenjen je udio malih kanida, dok je metodom fotozamki potcijenjen udio srna. Nadalje, metodom fotozamki je potcijenjen ukupan broj prijelaza životinja. Suprotno tome, IC senzorima je zabilježeno od 11 do 19 puta više prijelaza nego fotozamkama. Međutim, oko 80% IC događaja bili su artefakti, odnosno nisu ih uzrokovali prijelazi životinja. Stoga je razvijen algoritam za korekciju IC podataka kako bi se dobila točnija procjena stvarnog broja prijelaza. Zaključno, prikazani rezultati predstavljaju vrijedan doprinos jer daju pregled prednosti i nedostataka različitih metoda monitoringa te će doprinijeti većoj točnosti rezultata praćenja prijelaza divljih životinja preko zelenih mostova. Ključne riječi: zeleni mostovi, monitoring prijelaza životinja, usporedba metoda ANALYSIS OF METHODS FOR WILDLIFE MONITORING ON GREEN BRIDGES G. Gužvica1, I. Bošnjak2, A. Bielen3, D. Babić4, B. Radanović‐Gužvica5, L. Šver3 th
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Group for large carnivores, Geonatura Ltd., Zagreb, Croatia ([email protected]) University of Zagreb, Faculty of Science, Department of Botany, Zagreb, Croatia 3 University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Zagreb, Croatia 4 Byte Lab, Zagreb, Croatia 5 Croatian Natural History Museum, Zagreb, Croatia Green bridges mitigate negative impact of roads/highways on wildlife populations. Their effectiveness is evaluated by various monitoring methods. Based on the 3‐year monitoring period of four green bridges on A1 motorway in Croatia, the effectiveness of three monitoring methods was compared: track‐pads, camera traps and active infrared (IR) trail monitoring system. The methods were evaluated in terms of their ability to detect different species and to give good estimation of the number of animal crossings. The accuracy of species detection by track‐pad method was influenced by granulometric composition of the track‐pad material, with the best results obtained when higher percentages of silt and clay were used. Regarding the species composition, monitoring by tracks underestimated the ratio of small canids, while camera traps underestimated the ratio of roe deer. Furt her, camera trap method underestimated the real number of animal crossings. On the contrary, active IR detectors recorded from 11 to 19 times more events than camera traps. However, circa 80% of IR events were not caused by wildllife crossings. Therefore, an algorithm for correction of the IR dataset was developed, in order to approximate the real number of crossings. In conclusion, presented results are valuable for future monitoring of wildlife crossings since they provide advantages and disadvantages of different monitoring methods. Key words: green bridges, wildlife crossing monitoring, comparison of methods P‐5 PRELIMINARNI REZULTATI ISTRAŽIVANJA VUKOVA U PARKU PRIRODE BIOKOVO G. Gužvica1,2, I. Gabrić3, K. Protrka3, Z. Grgurić1, L. Šver4 1 Grupa za velike zvijeri, Geonatura d.o.o., Zagreb, Hrvatska ([email protected], [email protected]) 2 Zavod za kopnene ekosustave, Oikon d.o.o., Institut za primijenjenu ekologiju, Zagreb, Hrvatska 3Javna ustanova 'Park prirode Biokovo', Makarska, Hrvatska 4 Zavod za biokemijsko inženjerstvo, Prehrambeno‐biotehnološki fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska U okviru projekta 'Istraživanje i monitoring vukova u Parku prirode Biokovo' od 2013. godine provodi se istraživanje s ciljem utvrđivanja prisutnosti, brojnosti i stabilnosti čopora vukova te utvrđivanja veličine područja obitavanja čopora. U istraživanju se primjenjuju metode fotozamki, praćenja tragova, akustična metoda i metoda GPS telemetrije te analiza strukture prehrane. Tijekom prvih 18 mjeseci istraživanja postavljene su fotozamke na 20 lokacija te su prikupljene 3922 fotografije pogodne za analizu. Vuk je snimljen na 5 različitih lokacija na 46 fotografija odnosno 1,17% slika. Na istoj fotografiji su zabilježene najviše dvije jedinke vuka. Na temelju tragova ili izmeta prisutnost vuka utvrđena je na 25 lokacija te je prikupljeno 53 izmeta pogodnih za analizu strukture prehrane. Provedbom akustične metode odaziv vukova zabilježen je na osam lokacija. Samo na jednoj lokaciji utvrđen je odaziv najmanje 5 jedinki. Provedba GPS telemetrije je u fazi hvatanja i obilježavanja jedinke vuka. Na temelju dosadašnjih rezultata još nije moguće izvesti pouzdane zaključke o broju čopora i brojnosti 2
th
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jedinki vuka u Parku prirode Biokovo. Rezultati akustične metode ukazuju na najmanje pet jedinki u čoporu, dok ostale metode ukazuju na pojedinačno pojavljivanje vukova kao privremeno ili trajno dispergiranih jedinki ili ostatku 'razbijenog' čopora što je moguće posljedica značajnog pritiska na vuka od strane lovaca i stočara. Ključne riječi: sivi vuk, Canis lupus, Park prirode Biokovo THE RESEARCH OF WOLVES IN THE NATURE PARK BIOKOVO – PRELIMINARY RESULTS G. Gužvica1,2, I. Gabrić3, K. Protrka3, Z. Grgurić1, L. Šver4 1 Group for large carnivores, Geonatura Ltd., Zagreb, Croatia ([email protected], [email protected]) 2 Department for terrestrial ecology, Oikon Ltd., Institute for Applied Ecology, Zagreb, Croatia 3 Public Institution 'Nature Park Biokovo', Makarska, Croatia 4 Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia From 2013, the project 'Research and monitoring of wolves in the Nature Park Biokovo' is being conducted to determine the presence, abundance and stability of the wolf packs and to determine the size of their home range. Monitoring methods being applied are: digital camera traps, recording of footprints and scat, acoustic method, GPS telemetry and analysis of the diet. During the first 18 months of research, camera traps were placed at 20 locations and 3922 photos suitable for analysis were collected. Wolves were recorded in 46 photos (1.17%) at 5 different locations. No more than two wolves were recorded in the same photograph. According to observed footprints or scats, the presence of wolf was determined at 25 locations and 53 scats suitable for diet analysis were collected. Using acoustic method, howling reply was recorded at 8 locations while the reply of at least five individuals is recorded only in one location. GPS telemetry is at the stage of capturing and marking an individual. Based on the preliminary results it is not possible to draw reliable conclusions about the number of packs and number of wolf’s individuals in the Nature Park Biokovo. Results of acoustic method indicate at least five individuals in a pack, while other methods point to appearance of individuals as a temporary or permanent dispersed individuals or the remainder of the 'broken' pack which could be the result of a significant influence on the wolf by hunters and stockbreeders. Key words: grey wolf, Canis lupus, Nature Park Biokovo P‐6 NOVA STRANA VRSTA DESETERONOŽNIH RAKOVA (MRAMORNI RAK (Procambarus fallax (Hagen, 1870) f. virginalis) U SLATKOVODNIM EKOSUSTAVIMA HRVATSKE S. Hudina1, M. Samardžić2, A. Lucić1, M. Jelić1, I. Maguire1 1 Zoologijski zavod, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) 2 Gimnazija 'Fran Galović', Koprivnica, Hrvatska Mramorni rak (Procambarus fallax (Hagen, 1870) f. virginalis) treća je alohtona vrsta slatkovodnih deseteronožnih rakova koja zabilježena u vodotocima Hrvatske. Ova partenogenetska vrsta raka zabilježena je prvi put 2013. godine u šljunčari Šoderica na sjeveru Hrvatske. Tijekom 2015. godine ispitali smo potencijalne putove unosa mramornog raka u Hrvatsku, te istražili osnovne značajke populacije zabilježene u šljunčari Šoderica (relativna th
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gustoća rakova, uzrasna struktura populacije, značajke godišnjeg ciklusa) i potencijal daljnjeg širenja ove vrste na području Međimurja i Podravine. Kako bi upravljanje i aktivnosti usmjerene na kontrolu širenja ove vrste bile pravovremene, neophodni su detaljni podaci o strukturi i dinamici populacije mramornog raka u Šoderici. Ključne riječi: slatkovodne invazije, partenogenetska vrsta, populacijske značajke, invazivni areal NEW NON‐INDIGENOUS CRAYFISH (THE MARBLE CRAYFISH (Procambarus fallax (Hagen, 1870) f. virginalis) IN CROATIAN FRESHWATERS S. Hudina1, M. Samardžić2, A. Lucić1, M. Jelić1, I. Maguire1 1 Division of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) 2 Gymnasium “Fran Galović”, Koprivnica, Croatia The marble crayfish (Procambarus fallax (Hagen, 1870) f. virginalis) is the third non‐indigenous crayfish species established in Croatian freshwater habitats. This parthenogenetic species was discovered in 2013 in the Šoderica gravel pit lake, located in the north of Croatia. In this study we looked into potential introduction pathways of the marble crayfish to Croatia, examined some of its basic population characteristics (i.e. relative crayfish abundance, population size structure and year cycle characteristics) and analysed its potential for dispersal in the region. Research and monitoring programme for the Šoderica marble crayfish population is urgently required in order to immediately start with management actions targeted at its control. Key words: freshwater invasion, parthenogenetic species, population characteristics, invasive range P‐7 SMJERNICE ZA PROCJENU UTJECAJA VJETROELEKTRANA NA VELIKE ZVIJERI J. Kusak1, Đ. Huber1, N. Trenc2, J. Jeremić2, S. Desnica2 1 Zavod za biologiju, Veterinarski fakultet Sveučilište u Zagrebu, Zagreb 2 Državni zavod za zaštitu prirode, Zagreb ([email protected] ) Razvoj vjetroparkova u Hrvatskoj velikim je dijelom zbog odgovarajućeg vjetropotencijala usmjeren na Dinarsko područje koje ujedno predstavlja i vrijedno stanište velikih zvijeri (vuka, medvjeda i risa). Najveću prijetnju opstanku velikih zvijeri predstavljaju fragmentacija i gubitak staništa, a vjetroelektrane s pripadajućim pristupnim putovima značajno doprinose ovom procesu. S obzirom na potrebu da se u adekvatno ocijene utjecaji vjetroparkova u različitim postupcima sukladno propisima iz područja zaštite prirode i okoliša te EU direktivama, Zavod za biologiju Veterinarskog fakulteta u Zagrebu i Državni zavod za zaštitu prirode zajednički su pristupili izradi stručnih Smjernica za procjenu utjecaja vjetroelektrana na velike zvijeri. Dosadašnja praksa pokazala je da je za smanjivanje i izbjegavanje pojedinačnih samostalnih i kumulativnih utjecaja vjetroparkova ključan njihov odgovarajući smještaj u prostoru. Stoga Smjernice predlažu metodologiju ocjene temeljenu na karti pogodnosti staništa koja putem matematičkog modela i GIS‐a objedinjava informacije o opažanjima velikih zvijeri s informacijama o osobinama staništa te pokazuje koliko je određeno područje značajno za pojedinu vrstu velikih zvijeri. Izdvojeno je devet kategorija pogodnosti staništa, te su th
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postavljeni postotni i apsolutni limiti daljnjeg gubitka za svaku kategoriju. Također je obrazložen način kako koristiti kartu u različitim postupcima ocjene. Ključne riječi: vjetroelektrane, velike zvijeri, ocjena utjecaja, smjernice, GIS GUIDELINES FOR ASSESSMENT OF WIND FARMS IMPACT ON LARGE CARNIVORES J. Kusak1, Đ. Huber1, N. Trenc2, J. Jeremić2, S. Desnica2 1 Biology Department of Veterinary Faculty, University of Zagreb, Zagreb 2 State Institute for Nature Protection, Zagreb ([email protected] ) The Dinaric mountain range due to its wind potential is the preferred area for wind farm development in Croatia but in the same time it represents a valuable habitat for large carnivores (wolf, bear and lynx). The biggest threat to large carnivores’ survival is habitat fragmentation and loss. Wind farms with access roads and associate disturbance significantly contribute to that process. To ensure adequate assessment of wind farm impacts in different environmental and nature protection procedures pertaining to national and EU legislation, Biology Department of Veterinary Faculty in Zagreb and State Institute for Nature Protection jointly developed Guidelines for assessment of wind farms impact on large carnivores. The experience shows that the best approach for reduction and avoidance of individual and cumulative impacts of wind farms is their appropriate spatial p ositioning. Therefore the Guidelines propose an assessment methodology based on the sensitivity map, which was created throughout mathematical modeling and GIS using data on large carnivores’ occurrences and related habitat characteristics. The sensitivity map indicates importance of certain area for each species of large carnivores and defines nine categories of significance. Also percent and absolute limits of further loss for each category have been determined. In addition, the appropriate use of sensitivity map in as assessment procedures is described and proposed. Key words: wind mills, large carnivores, impact assessment, guidelines, GIS P‐8 MODIFICIRANI LESLIE‐LEFKOVITCH GENETIČKI ALGORITAMSKI MODELSKI SUSTAV DINAMIKE POPULACIJA KOMARACA Ž. Lončarić, M. Jambrović, B. K. Hackenberger Zavod za kvantitativnu ekologiju, Odjel za biologiju Sveučlišta Josipa Jurja Strossmayera, Osijek, Hrvatska ([email protected]) Fluktuacije veličine i promjene starosne strukture populacija komaraca tijekom godine važni su parametri nužni za planiranje i provođenje mjera kontrole brojnosti komaraca na nekom području. Osim toga, dinamika populacija komaraca je i izuzetno zanimljivo akademsko pitanje zbog njenih osobina kao što su podložnost Allee‐efektu i periodična kaotičnost. Upravo zbog tih osobina je dinamiku populacija komaraca teško predviđati temeljem modela načinjenih uobičajenim tehnikama. Do sada su najbolje rezultate u modeliranju dinamike populacija komaraca pokazali modificirani diskretni Leslie‐Lefkovitch matrični modeli (MLL). Kako bi se poboljšala svojstva predikcije rezultata tretiranja komaraca larvicidima i adulticidima te doprinijelo izradi računalne podrške sustavu odlučivanja, u ovom radu su se postojećim modelima dodali programski moduli na temelju gen etičkih algoritama. Tako dobiveni hibridni th
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sustav iskoristio se za dobivanje serija predikacija koje su uspoređene s predikcijama dobivenim pomoću jednostavnog MLL‐sustava. Osim u poboljšanju prediktivnosti rezultata tretiranja, hibridni sustav je pokazao i nova aplikativna svojstva modela koja znatno doprinose računalnom upravljanju kontrole komaraca. Ključne riječi: komarci, genetički algoritam, MLL, predikcija MODIFIED LESLIE‐LEFKOVITCH‐GENETIC ALGORITHM HYBRID MODELS SYSTEM OF MOSQUITO POPULATION DYNAMIC Ž. Lončarić, M. Jambrović, B. K. Hackenberger Department of Biology, Josip Juraj Strossmayer University Osijek, Osijek, Croatia ([email protected]) Fluctuations in size and changes in age structure of mosquito populations during the year are important parameters necessary for planning and implementation of mosquito control measures. Additionally, mosquito population dynamic is an exceptionally interesting academic question due to its periodic chaos system and susceptibility to Allee‐effect. It is those characteristics that make mosquito population dynamic difficult to predict using the usual methods. Until now, discrete Leslie‐Lefkovitch matrix models (MLL) showed the best results in mosquito population dynamic modelling. To improve result prediction properties of larvicides and adulticides, and to contribute to the development of computer support to the deciding system, program modules based on genetic algorithms were added to the existing models. This hybrid system was used to make a series of predictions whic h were compared to the predictions made by simple MLL‐system. Hybrid system, aside from improving prediction of treatment results, showed new applicable model properties which significantly contribute to computer management of mosquito control. Key words: mosquitoes, genetic algorithm, MLL, predictions P‐9 ŠIŠMIŠI NA BRIJUNIMA – REZIDENCIJALNA ELITA ILI MASOVNI TURIZAM? M. Mazija, Z. Domazetović, B. Benčina, A. Štefan, Ž. Drdar Udruga za zaštitu šišmiša Tragus, Zagreb, Hrvatska ([email protected]) Prikupljanje prvih podataka o fauni šišmiša Nacionalnog parka Brijuni započelo je preliminarnim istraživanjem u rujnu 2010. godine. Pretražen je veći broj potencijalnih skloništa i provedena pregledna istraživanja hvatanjem mrežama i snimanjem ultrazvučnim detektorom. Već prvi podaci pokazali su iznenađujuću brojnost jedinki i raznolikost vrsta. Iz tog razloga su u razdoblju od 2011. do 2014. nastavljena istraživanja na većem broju objekata te na odabranim staništima značajnim za šišmiše. Istraživanja su provedena na Velikom i Malom Brijunu, ali i na drugim manjim otocima Brijunskog arhipelaga. Hvatanjem mrežama, snimanjem ultrazvučnim detektorom i pretragom objekata utvrđeno je ukupno 14 vrsta šišmiša. Značajno je istaknuti prisutnost porodiljne kolonije vrste Myotis nattereri, jedinke vrste Myotis mystacinus u hibernaciji na otoku Mali Brijun te uhvaćenog mužjaka vrste Nyctalus lasiopterus s izraženim testisima i bukalnim žlijezdama, što je tek drugi nalaz žive jedinke ove vrste u Hrvatskoj nakon 89 godina. Analiza sonograma ukazuje na područja s većom brojnošću vrsta i gustoćom jedinki te na prostore koje koriste vrste od osobitog značaja. Temeljem analize rezultata istraživanja, th
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dani su prijedlozi mjera zaštite šišmiša i podržavanja populacija na području NP Brijuni. Obzirom na značaj dosad prikupljenih podataka, daljnja istraživanja temeljit će se na usmjerenom praćenju najvažnijih elemenata (staništa i vrsta) u razdobljima značajnim za godišnji ciklus šišmiša. Ključne riječi: šišmiši, Nacionalni park Brijuni BATS ON BRIJUNI – RESIDENTIAL ELITE OR MASS TOURISM? M. Mazija, Z. Domazetović, B. Benčina, A. Štefan, Ž. Drdar Association for Bat Conservation Tragus, Zagreb, Croatia ([email protected]) Initial data gathering on bat fauna in Brijuni National Park began with preliminary research in September 2010. Many potential sites were examined and screening was conducted by catching with mist nets and recording with ultrasound detector. The first data indicated a surprising number of individuals and great species diversity. For this reason, during the period between 2011 and 2014, research was extended to a greater number of sites and selected habitats important to bats. The research was conducted on Veliki and Mali Brijun, as well as on other smaller islands within the Brijuni archipelago. Altogether, 14 bat species were found by mist netting, recording with ultrasound detectors and examination of sites. It is important to emphasise the presence of Myotis nattereri maternity colony, hibernating Myotis mystacinus individuals on island Mali Brijun and captured Nyctalus lasiopterus male, with enlarged testes and buccal glands, which is the second finding of a live individual of this species in Croatia after 89 years. Sonogram analysis reveals areas with greater number of species and density of individuals, as well as areas used by species of importance. Based on the analysis of the results, measures to protect bats and support bat populations in Brijuni National Park were suggested. Given the importance of the collected data, further research will be focused on monitoring of the key elements (habitats and species) in the periods important for the annual cycle of bats. Key words: bats, Brijuni National Park P‐10 RASPROSTRANJENOST I TREND GNIJEZDEĆE POPULACIJE VRANE GAČAC (Corvus frugilegus L. 1758) NA PODRUČJU OSIJEKA A. Mikuška1, A. Felja1, D. Bjedov2, K. Kajan2, M. Blažević2, A. Zovko2, P. Benić2 1 Zavod za zoologiju,Odjel za biologiju, Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek ([email protected]) 2 Udruga studenata biologija ‐ ZOA, Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek Iako je u nedavnoj prošlosti bila tipična stepska ptica, vrana gačac (Corvus frugilegus) je danas sve brojnija u urbanim područjima. Cilj istraživanja je utvrđivanje trenda gnijezdeće populacije, te utvrđivanje veličine zimujuće populacije vrane gačca na području grada Osijeka. Prebrojavanje broja gnijezdećih parova gačaca u kolonijama obavljeno je metodom brojanja sigurno aktivnih gnijezda u kolonijama tijekom proljeća 2014. i 2015. godine, a tijekom zima gačci su brojani na okupljalištima prije odlaska na spavalište. Obradom literaturnih i podataka s terenskih istraživanja uz pomoć softverskog paketa TRIM izračunat je trend populacije u zadnje 23 godine. Tijekom zima 2014. i 2015. godine, na okupljalištima se prije spavanja nalazilo prosječno 4000 gačaca. Tijekom 2014. godine na području grada Osijeka gnijezdilo je 560 th
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parova gačaca u 13 kolonija, dok je u 2015. godini gnijezdilo 623 parova u 12 kolonija. Najveći broj kolonija (šest) pripada malim kolonijama, do 50 parova, u kojima je prosječno gnijezdilo 19 parova. Srednje velikih kolonija (50‐100 parova) je bilo između jedne (2014.) i četiri (2015.), a velikih kolonija sa više od 100 parova su tijekom istraživanog perioda je bilo samo dvije. Trend gnijezdeće populacije gačaca na području grada Osijeka tijekom 23 godine je umjereno opadajući. Glavni razlozi tomu jesu stalna rastjerivanja kolonija, orezivanje stabala na kojima su kolonije izgrađene, te rušenje njihovih gnijezda. Ključne riječi: vrana gačac, trend gnijezdeće populacije, Osijek DISTRIBUTION AND TREND OF BREEDING POPULATION OF ROOKS (Corvus frugilegus L.1758) IN OSIJEK A. Mikuška1, A. Felja1, D. Bjedov2, K. Kajan2, M. Blažević2, A. Zovko2, P. Benić2 1 Subdepartment of zoology,Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia ([email protected]) 2 Biology Students Association – ZOA, Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek Despite the fact that Rook was a typical bird of the steppe habitats, their numbers are nowadays increasing in urban areas. The aim of this study is to present the trend of breeding population and size of wintering population of Rook in Osijek town. Breeding population was established by counting Apparently Occupies Nest in Rook colonies during springs. Wintering population was studied by counting Rook at their roosts during 2014 and 2015. Software package TRIM was used to calculate the breeding population trend for last 23 years. On winter roosting localities 4000 Rooks were counted in average during winters of 2014 and 2015. During 2014 560 breeding pairs were counted in 13 colonies, while 623 breeding pairs were breeding in 12 colonies during 2015. Majority of the colonies (6) were classified as small (below 50 pairs), with average of 19 nesting pairs. Number of medium‐sized colonies (50‐100 pairs) varied between one in 2014 and four in 2015, while two big colonies (over 100 pairs) were present in both years. In the past 23 years the breeding population trend shows moderate decline, mainly because of the harassment of the colonies, nesting sites destruction and disturbance of breeding Rooks. Key words: Rook, breeding population trend, Osijek P‐11 OPTIMIZACIJA METODA ZA MOLEKULARNO ODREĐIVANJE SPOLA KOD ČAPLJI (ARDEIDAE) A. Mikuška, L. Begović, I. Mihić, K. Kljajić, R. Grgac Zavod za zoologiju, Odjel za biologiju, Sveučilište J. J. Strossmayer u Osijeku, Osijek, Hrvatska ([email protected]) Genetski markeri su koristan alat za praćenje promjena u populacijskim studijama. Cilj rada je optimiziranje metode za izolaciju DNA i određivanje spola molekularnim metodama kod čaplji (Ardeidae). Genomska DNA je izolirana iz perja i ljuski jaja koristeći dva različita protokola. Za određivanje spola korištene su početnice 2550F/2781R za kromo‐helikazu DNA‐vezujući gen (CHD). Rezultati su pokazali da je najveći prinos DNA dobiven iz pera koja su u razvoju (prokrvljena pera) i ljuski jaja. Prinos DNA je bio manji kad se koristio komercijalni kit za izolaciju u odnosu na izolaciju s TNE puferom uz dodatak natrijevog acetata za taloženje th
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keratina. Ključan korak u izolaciji je koncentracija ditiotreitola (DTT) i proteinaze K u puferu za liziranje kao i količina krvi prisutna u perju. Ključne riječi: genetski markeri, određivanje spola, čaplje OPTIMIZATION OF METHODS FOR MOLECULAR SEX TYPING IN HERONS (ARDEIDAE) A. Mikuška, L. Begović, I. Mihić, K. Kljajić, R. Grgac Subdepartment for Zoology, Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia ([email protected]) Genetic markers are useful tool for tracking changes in population studies. This work, is focused on optimization of method for DNA isolation and molecular sex determination in herons (Ardeidae). Genomic DNA was isolated from feathers and eggshells by using two different protocols. For sex‐typing of herons we used primers 2550F/2781R for chromo‐ helicase–DNA binding gene 1 (CHD1). Results showed that the highest DNA yield was obtained from the blood feathers and eggshells while mature feathers had the lowest yield. DNA yield was lower when commercial kit was used for isolation compared to the isolation with TNE buffer followed by addition of sodium acetate for keratin precipitation. Critical step is concentration of dithiothreitol (DTT) and proteinase K in lysis solution as well as the amount of blood present in the feathers. Key words: genetic markers, sex‐typing, herons P‐12 BIOLOŠKA ZAŠTITA PITOMOG KESTENA GLJIVAMA ANTAGONISTIMA S. Novak Agbaba1, I. Franić2 1 Zavod za zaštitu šuma i lovno gospodarenje, Hrvatski šumarski institut, Jastrebarsko, Hrvatska ([email protected]) 2 Cuglini 1, Zagreb, Hrvatska Sastojine pitomog kestena ugrožene su od dva invazivna organizma, gljive Cryphonectria parasitica i ose šiškarice Drycosmus kuriphilus. Gljiva C. parasitica uzročnik je raka kore, a osa šiškarica D. kuriphilus oštećuje pupove i listove te uzrokuje formiranje šiški u krošnji. Na šiškama, granama i plodu nalazimo gljivu C. parasitica, saprotrofne i druge parazitske gljive. Osim njih, nalazimo i gljive antagoniste, najčešće Trichoderma viride, T. citrinoviride i Trichothecium roseum. Cilj ovog istraživanja bio je utvrditi djelovanje gljiva antagonista na rast gljive C. parasitica u svrhu biološke zaštite pitomog kestena. Da bismo utvrdili odnos gljiva antagonista T. viride, T. citrinoviride i T. roseum prema gljivi C. parasitica, postavljen je laboratorijski pokus. T. viride je ostvarila kontakt 4.‐5. dan, a prerastanje 6.‐10. dan. T. citrinoviride je ostvarila kontakt 4.‐6. dan, a prerastanje i djelomično prerastanje 25.‐46. dan. T. roseum je ostvarila kontakt 9.‐11. dan, a prerastanje 15.42. dan. T. viride ima najbrži rast i najjače antagonističko djelovanje. Gljive antagonisti T. viride, T. citrinoviride i T. roseum sveprisutne su u kestenovoj sastojini. Laboratorijskim pokusom dokazano je da navedene gljive antagonisti djeluju inhibicijski prema C. parasitica. To ukazuje na to da je u sastojinama pitomog kestena prisutna prirodna biološka zaštita gljivama antagonistima. Ključne riječi: pitomi kesten, Cryphonectria parasitica, gljive antagonisti, biološka zaštita th
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BIOLOGICAL CONTROL OF SWEET CHESTNUT WITH ANTAGONISTIC FUNGI S. Novak Agbaba1, I. Franić2 1 Division for forest protection and game management, Croatian forest research institute, Jastrebarsko, Croatia ([email protected]) 2 Cuglini 1, HR‐ 10000 Zagreb, Croatia Chestnut stands are threatened by two invasive organisms, fungus Cryphonectria parasitica and gall wasp Drycosmus kuriphilus. The fungus C. parasitica cause cancer bark disease. Gall wasp D. kuriphilus cause the damage on buds and leaves and form galls in the crown. On the galls, branches and nut C. parasitica, saprophytic and parasitic fungi were found. Apart from them antagonistic fungi, usually Trichoderma viride, Trichoderma citrinoviride and Trichothecium roseum were found. The aim of this study was investigate the effect of the antagonistic fungi on C. parasitica growth and possibility of biological control. To determine the interaction between antagonistic fungi T. viride, T. citrinoviride and T. roseum and C. parasitica the laboratory test were established. T. viride has made contact on days 4‐5, and was overgrown by C. parasitica within 6‐10 days. T. citrinoviride has made the contact on days 4‐6, and was overgrown or partial overgrown within 25‐46 days. T. roseum has made contact on day 9‐11, and was overgrown within 15‐29‐42 days. T. viride has the fastest grow and the most powerful antagonistic activity. Antagonistic fungi T. viride, T. citrinoviride and T. roseum are ubiquitous in chestnut stands. Laboratory experiment demonstrated that the antagonistic fungi were inhibited C. parasitica. This indicates that in the chestnut stands natural biological control with antagonistic fungi is present. Key words: sweet chestnut, Cryphonectria parasitica, antagonistic fungi, biological control P‐13 SOLARNI PARK KOSORE – UTJECAJI NA OKOLIŠ I EKOLOŠKU MREŽU N. Pavlus, M. Drakšić, D. Švaljek, M. Perčić, D. Šegvić, M. Vuković, B. Matošić, S. Blažević Zeleni servis d.o.o., Split, Hrvatska ([email protected]) Solarni park Kosore graditi će se na području radne zone Kosore, na području grada Vrlike. Planirana je gradnja sedam različitih fotonaponskih elektrana na tlu, svaka snage 300 kW. Zahvat će omogućiti proizvodnju električne energije, koja će se distribuirati u elektroenergetsku mrežu. Obzirom da se radi o solarnom parku u kojem se prezentira sedam različitih tehnologija, može poslužiti kao ogledni prostor za optimiranje učinkovitoski tehnologije u odnosu na utjecaje na okoliš i ekološku mrežu, za potrebe budućih solarnih parkova na sličnim lokacijama u Republici Hrvatskoj.
Ključne riječi: sunčane elektrane, sedam različitih tehnologija, utjecaji, okoliš, ekološka mreža
SOLAR PARK KOSORE – ENVIRONMENTAL IMPACT AND NATURA 2000 NETWORK N. Pavlus, M. Drakšić, D. Švaljek, M. Perčić, D. Šegvić, M. Vuković, B. Matošić, S. Blažević Zeleni servis d.o.o., Split, Croatia ([email protected]) Solar park Kosore will be build within Kosore work zone, in the town of Vrlika. It is planned to build seven different photovoltaic power plants on the ground, each rated at 300 kW. The th
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procedure will allow production of electricity, which will then be distributed in to the electricity network. Given that this is a solar park which represents seven different technologies and it can be used as a prime area for technology effectivenes optimization in relation to the impacts on the environment and Natura 2000 network, and also for the future solar parks in similar locations in the Republic of Croatia.
Key words: solar power stations, seven different technologies, impacts, environment, Natura 2000 network
P‐14 SIDRIŠTE U UVALI MEZUPORAT, OTOK BIŠEVO – KAKO SAČUVATI EKOLOŠKU MREŽU?
N. Pavlus1, M. Perčić1, M. Drakšić1, M. Vuković1, S. Blažević1, Ž. Varga2, A. Ptiček2, H. Čizmek3 1 Zeleni servis d.o.o., Split, Hrvatska ([email protected]) 2 INSTITUT IGH, d.d. Zagreb, Hrvatska 3 JANOLUS d.o.o., Zadar, Hrvatska
Sidrište u uvali Mezuporat, na otoku Biševu je planirano za 20 brodova, na površini akvatorija od 4500 m2, koji je dio ekološke mreže HR3000098 Biševo more. Sidrište se nalazi u blizini Modre špilje – jedinog od rijetkih zaštićenih objekta te vrste na Jadranu koji se posjećuje i dio je ekološke mreže, HR3000184 Modra špilja. Razlog za izgradnju sidrišta je pritisak posjetitelja na Modru špilju, koji raste iz godine u godinu, uslijed čega u ljetnim mjesecima cijela uvala postaje prostor sve brojnijeg 'divljeg' sidrenja brodova, čime se sustavno uništava morsko dno, zaštićene biljne i životinjske vrste, kao dio ekološke mreže Republike Hrvatske. Ključne riječi: Modra špilja, uvala Mezuporat, posjetitelji, utjecaji, ekološka mreža, sidrište THE ANCHORAGE IN THE MEZUPORAT BAY, ISLAND OF BIŠEVO ‐ HOW TO PRESERVE NATURA 2000 NETWORK? N. Pavlus1, M. Perčić1, M. Drakšić1, M. Vuković1, S. Blažević1, Ž. Varga2, A. Ptiček2, H. Čizmek3 1 Zeleni servis d.o.o., Split, Croatia ([email protected]) 2 INSTITUT IGH, d.d. Zagreb, Croatia 3 JANOLUS d.o.o., Zadar, Croatia The anchorage in the Mezuporat bay, on the island of Biševo is planned for 20 ships, in the aquatorium measuring 4500 m2 of surface of which is the part of Natura 2000 network HR3000098 Biševo more. The anchorage is located near the cave Modra ‐ one of the fewest protected areas of this kind in the Adriatic, which is frequently visited and it is part of the Natura 2000 network HR3000184 Modra špilja.The reason for building the anchorage is the rising visitor pressure on to the Modra cave, which is growing each year, resulting in the impact of the 'wild' ships anchoring through in the whole bay area in the summer months which systematically destroys the seabed, protected plant and animal species all being a part of Croatia Natura 2000 network. Key words: Modra cave, Mezuporat bay, visitors, impacts, Natura 2000 network, anchorage th
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P‐15 KVALITETA VODE U VODOTOCIMA POLJOPRIVREDNOG, MOČVARNOG I ŠUMSKOG PORJEČJA V. Peršić, A. Kočić, J. Horvatić Zavod za biokemiju i ekofiziologiju biljaka, Odjel za biologiju, Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska ([email protected]) Procjena ograničavajućih čimbenika rasta algi i trofičkog stanja vodotoka i poplavnog područja, koja karakteriziraju nepravilni hidrološki uvjeti i promjene vodostaja, uključuje različite pristupe potrebne za prihvatljivo razumijevanje problema eutrofikacije. Cilj ovog rada je pružiti informacije o prostornoj i vremenskoj raspodjeli hranjivih tvari i ograničavajućih čimbenika rasta algi, kako bi se stvorila znanstvena osnova za procjenu stanja trofije kao nužnog preduvjeta definiranja mjera za smanjenje opterećenja voda hranjivim tvarima. Za postizanje tog cilja dizajniran je eksperiment u svrhu kvantitativne procjene rasta algi nakon dodatka hranjivih tvari, a kao rezultat dobivena je mjerljiva vrijednost ograničavajućeg djelovanja određene hranjive tvari. Način korištenja zemljišta kao i vrijeme uzorkovanja imali su značajan utjecaj na utvrđe nu varijabilnost fizikalno kemijskih čimbenika, kao i potencijala rasta algi. U skladu s tim, kvaliteta voda u slivu s najvišim postotkom poljoprivrednog zemljišta bila je vrlo loša s iznimno visokim koncentracijama fosfora. Samo blagi znakovi odstupanja od referentnih vrijednosti zabilježeni su u vodotocima pretežno šumskog sliva kao rezultat antropogenih aktivnosti. S druge strane, u vodotocima močvarnih slivnih područja povremeno je utvrđen i nedostatak hranjivih tvari. Ključne riječi: eksperiment obogaćivanja nutrijentima, trofički uvjeti, N/P omjer WATER QUALITY OF WATERCOURSES DRAINING AGRICULTURAL, WETLAND AND FORESTED CATCHMENTS V. Peršić, A. Kočić, J. Horvatić Sub Department of Plant Ecophysiology and Biochemistry/Department of Biology, University of Osijek, Osijek, Croatia ([email protected]) Assessment of resources that are limiting algal growth and evaluation of the trophic status of rivers and wetlands, which present hydrological irregularities, incorporates different approaches in order to provide acceptable understanding of the problem. Our goal was to provide information on the temporal and spatial variation of resource limitation of algal growth rates to create a scientific basis for evaluating regional strategies of nutrient management in the investigated catchments. Therefore, a responsive test was designed to assess quantitatively algal growth after nutrient additions in order to obtain a biologically relevant measure of nutrient deficiency. As a result, a bioassay method was introduced for the evaluation of trophic status and limiting nutrients in the investigated watercourses. The observed variability in many of the physical and chemical const ituents, as well as algal growth potential, was attributed to the significant influence of both catchment land use and sampling time. Accordingly, the water quality of watercourses in the catchment with the highest percentage of arable land was determined to be very bad with extremely high phosphorus concentrations. Only moderate signs of distortion were observed in flowing waters of forested catchments as a result of anthropogenic activities. On the other hand, watercourses draining wetland watersheds showed even some nutrient deficiency. Key words: nutrient enrichment experiment, trophic conditions, N/P ratio th
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P‐16 EKOLOŠKA MREŽA NATURA 2000 ‐ ŠTO SE PROMIJENILO NAKON BIOGEOGRAFSKOG SEMINARA? I. Plavac, A. Duplić, K. Jelić, J. Radović, R. Topić, I.Ilijaš Državni zavod za zaštitu prirode, Zagreb, Hrvatska ([email protected]) Ekološka mreža Natura 2000 je mreža očuvanih područja Europe, namijenjena očuvanju više od 1000 ugroženih divljih vrsta i oko 230 stanišnih tipova. Dva su propisa temelj politike EU za postizanje očuvanja bioraznolikosti – Direktiva o staništima i Direktiva o pticama. Pristupanjem EU, Hrvatska je predložila Natura 2000 područja za više od 250 vrsta i 70 stanišnih tipova značajnih za Uniju, koji se pojavljuju u Hrvatskoj i time značajno doprinijela zaustavljanju gubitka bioraznolikosti na razini Europe. Područja mreže odabrana su na temelju stručnih kriterija za pojedinu vrstu i stanišni tip, prošla su proces konzultacija s javnošću te ih je Uredbom u 2013. (NN 124/13) donijela Vlada RH. Kako bi zadovoljila zahtjeve Direktive o staništima, Hrvatska je morala proći postupak potvrđivanja mreže, koji se provodi u vidu Biogeografskog seminara i za Hrvatsku je proveden u rujnu 2014. Na Seminaru su, osim predstavnika DZZP‐a kao koordinatora inventarizacije divljih vrsta i stanišnih tipova i predstavnika Europske komisije, sudjelovali i predstavnici NVO te neovisni stručnjaci. Temeljem zaključaka EK sa Seminara, DZZP usklađuje popis Područja očuvanja značajnih za vrste i stanišne tipove te ih RH dostavlja EK. Ključne promjene u područjima ekološke mreže predstavljene su u ovom radu, a obuhvaćaju dopunu mreže područjima rijeke Korane i Save uzvodno od Zagreba te dopunu postojećih područja novim ciljnim vrstama i stanišnim tipovima. Ključne riječi: ekološka mreža, Natura 2000, Biogeografski seminar, Direktiva o staništima NATURA 2000 NETWORK ‐ WHAT HAS CHANGED AFTER THE BIOGEOGRAPHICAL SEMINAR? I. Plavac, A. Duplic, K. Jelic, J. Radovic, R. Topic, I.Ilijas State Institute for Nature Protection, Zagreb Croatia ([email protected]) Natura 2000 network is a network of conservation areas in Europe, aimed to conserve more than 1000 threatened species and about 230 habitat types. The cornerstones of EU policy for achieving biodiversity conservation are two Directives – Habitats Directive and Birds Directive. By joining EU, Croatia has proposed Natura 2000 areas for more than 250 species and 70 habitat types present in Croatia, thus significantly contributing to halting the biodiversity loss in Europe. The areas have been selected according to scientific benchmarks, undergone the process of public consultations and in 2013 promulgated with the Ordinance of Croatian Government. To meet the requirements of the Habitats Directive, Croatia went through the confirmation process, conducted in the form of Biogeographical Seminar, which was carried out in September 2014. Apart from the SINP representatives as the species and habitats inventory coordinator and the representatives of the European Commission, the representatives of NGOs as well as independent experts participated in the Seminar. On the basis of the EC conclusions from the Seminar, SINP is harmonising the Sites of Community Importance which the Republic of Croatia is submitting to the EC. The essential changes in Natura 2000 areas are presented in this paper and they include two new sites as an addendum to the network (River Korana and Upper Sava) as well as addition of new target species and habitat types to existing areas. Key words: Natura 2000 network, Biogeographical Seminar, Habitats Directive th
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P‐17 ISTRAŽIVANJE RIJETKIH I UGROŽENIH SVOJTI MALAKOFAUNE ISTRE I. Sabolić, V. Dorić, A. Dobrović, A. Orlović, P. Crnčan, M. Rezo Udruga studenata biologije, Prirodoslovno‐matematički fakultet, Zagreb, Hrvatska ([email protected]) Prilikom projekta inventarizacije malakofaune Istre provođenog od 2012. do 2014. godine zabilježen je veći broj rijetkih i ugroženih vrsta mekušaca. Među njima je prema stupnju ugroženosti odabrano pet vrsta od posebnog značaja ‐ slatkovodni školjkaši Unio mancus i Microcondylaea compressa, slatkovodni puž Istriana mirnae i kopneni puževi Vertigo angustior i Vertigo moulinsiana). Cilj istraživanja bio je detaljnije proučiti rasprostranjenost i stanje populacija odabranih vrsta na području Istre, odrediti čimbenike koji ih ugrožavaju i predložiti eventualne mjere zaštite. Prilikom terenskih istraživanja korištene su uobičajene metode prikupljanja kopnenih i slatkovodnih mekušaca. Materijal je nakon prikupljanja determiniran te su izrađene karte rasprostranjenosti za svaku vrstu. Rezultati su pokazali da su odabrane vrste na području Istre prisutne na malom broju lokaliteta, a u nekim slučajevima (Microcondylaea compressa i Istriana mirnae) radi se i o jedinom nalazištu vrste u Hrvatskoj. Čimbenici koji ugrožavaju istraživane vrste su zahvati na vodotocima, onečišćenja, melioracije i unos alohtonih vrsta mekušaca. U svrhu zaštite istraživanih vrsta potrebno je redovito provjeravati stanje njihovih populacija i neutralizirati ili smanjiti sve štetne čimbenike koji na njega djeluju. Ključne riječi: mekušci, ugrožene vrste, Microcondylaea compressa, Istriana mirnae, Istra RESEARCH OF RARE AND ENDANGERED TAXA OF ISTRIAN MALACOFAUNE I. Sabolić, V. Dorić, A. Dobrović, A. Orlović, P. Crnčan, M. Rezo Biology Students Association (BIUS), Faculty of Science, Zagreb, Croatia ([email protected]) During the project of molluscs inventarisation in Istria carried out between 2012 to 2014, a large number of rare and endangered species was recorded. Based on the level of threat they are facing, five of them were chosen for this study: the freshwater Bivalvia ‐ Unio mancus and Microcondylaea compressa, the freshwater Gastropoda ‐ Istriana mirnae, terrestrial Gastropoda ‐ Vertigo angustior and Vertigo moulinsiana. The purpose of the project was to examine the prevalence and population states of the chosen species in Istria, as well as determine the factors that present a treat to them, and suggest possible measures of protection. During the field work operations, usual methods for sampling of terrestrial and freshwater molluscs were used. The samples were determined and distribution maps were made for each species. The results show that the chosen species are present in a small number of locations in Istria, and for some of them (i.e. Microcondylaea compressa, and Istriana mirnae) this was the first time their presence was confirmed in Croatia. The factors that present a threat to the investigated species are interventions on the watercourses, polution, land reclamation, and alien molluscs species. For protection purposes, state of populations of these species should be examined regulary, and all harmful factors neutralized or reduced. Key words: molluscs, endangered species, Microcondylaea compressa, Istriana mirnae, Istria th
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P‐18 STRUKTURA I VIJABILNOST POPULACIJE SRNE, Capreolus capreolus U KONTINENTALNOJ HRVATSKOJ M. Sabolić1, I. Buj2, M. Mrakovčić2, M. Ćaleta3 1 Državni zavod za zaštitu prirode, Zagreb, Hrvatska 2 Zoologijski zavod, Biološki odsjek, PMF, Zagreb, Hrvatska ([email protected]) 3 Učiteljski fakultet, Zagreb, Hrvatska Kako bi predvidjeli buduće promjene u dinamici divljih populacija, osobito vjerojatnost izumiranja, potrebne su kvantitativne analize temeljene na podacima o ekološkim populacijskim značajkama. Ovo je istraživanje provedeno s ciljem određivanja ekoloških značajki populacije srne na lovištu u kontinentalnoj Hrvatskoj i kvantitativne procjene preživljavanja te populacije. Na temelju dobivenih rezultata zaključili smo da stanišna struktura i antropološke aktivnosti uvelike utječu na rasprostranjenost, populacijsku gustoću i preživljavanje populacije srne. Istraživana je populacija prometnicom podijeljena u dvije subpopulacije. Ukupan broj jedinki te gustoća i broj lanadi po ženki veći su u istočnoj subpopulaciji, vjerojatno kao posljedica bolje kvalitete staništa (većeg udjela šuma) na području njenog obitavanja. Usporedbom utvrđenih populacijskih parametara s literaturnim podacima dolazi se do zaključka da je kvaliteta staništa na cijelom istraživanom području visoka, a populacijska gustoća zadovoljavajuća, s obzirom da se radi o populaciji pod lovnim pritiskom. Rezultati analize vijabilnosti ukazuju na tanku liniju između stabilne populacije i izumiranja, naglašavajući važnost strogih kontrola i sprječavanja krivolova. Ključne riječi: srna, struktura populacije, procjena vijabilnosti, lovni pritisak STRUCTURE AND VIABILITY OF THE ROE DEER Capreolus capreolus POPULATION IN THE CONTINENTAL CROATIA M. Sabolić1, I. Buj2, M. Mrakovčić2, M. Ćaleta3 1 State Department for Nature Protection, Zagreb, Hrvatska 2 Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) 3 Faculty of Teacher Education, University of Zagreb, Zagreb, Croatia
In order to predict future alterations of the dynamics of wild animal populations, in particular the possibility of extinction, quantitative analyses based on the information on the ecological population parameters, are required. This investigation was conducted in order to estimate the population parameters of the Roe deer population on the hunting‐ground in northern Croatia and to quantitatively explore its viability. It was concluded that the habitat structure and anthropological activities have great effect on the distribution, population density and survival of the investigated population. That population is divided into two subpopulations by a traffic road. The total number of individuals, population density and the number of fawns per female are greater in the eastern subpopulation, probably due to the better habitat quality (greater portion of forests) in its distribution range. Nevertheless, by comparing the population parameters obtained with literature data, it can be concluded that the environment quality is high in the whole investigated area and that the population density is satisfactory for populations which are under hunting pressure. Results of the viability analyses indicate that the line between balanced population density and extinction is very thin for the Roe deer populations under the anthropological influence, implying the importance of rigorous control and prevention of illegal hunting. th
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Key words: Roe deer, population structure, viability estimation, hunting pressure
P‐19 OCJENA PROGRAMA RADOVA ODRŽAVANJA U PODRUČJU ZAŠTITE OD ŠTETNOG DJELOVANJA VODA U HRVATSKOJ ‐ UVJETI I MJERE ZAŠTITE PRIRODE: TEMELJI, IZAZOVI I PLANOVI ZA BUDUĆNOST
M. Sabolić1, T. Čuković2, A. Duplić1, P. Lunko1, D. Schneider1 1 Odjel za ocjenu prihvatljivosti zahvata za prirodu, Državni zavod za zaštitu prirode, Zagreb, Hrvatska ([email protected]) 2 Paljetkova ulica 2, Zagreb, Hrvatska
Radovi održavanja voda utječu na hidromorfološke i biološke elemente vodotoka što može znatno narušiti njihov ekološki status. Ukupna duljina prirodnih i umjetnih vodotoka u Hrvatskoj iznosi oko 32.100 km, a njima upravljaju Hrvatske vode. Zakonom o zaštiti prirode propisano je da planovi gospodarenja prirodnim dobrima sadrže uvjete zaštite prirode, a ocjena prihvatljivosti provodi se za one koji mogu imati značajan negativan utjecaj na ciljeve očuvanja i cjelovitost područja ekološke mreže. Suradnja vodnog i sektora zaštite prirode na Programu radova održavanja započela je 2011. godine podjelom radova u 11 grupa. Za njih je DZZP temeljem niza primjera dobre prakse iz literature do danas razradio 70 tipiziranih mjera zaštite i preporuka. Ocjenom Programa izdaju se mjere koje se odnose na ukupnu bioraznolikost te ciljne vrste i staništa područja ekološke mreže. Ovisno o ekološkim potrebama ciljnih vrsta osmišljene su specifične mjere. Od 2012. godine problemi kod ocjene Programa su kasno dostavljanje zahtjeva što dovodi do slučajeva da se radovi počnu izvoditi prije izdanih uvjeta, nedostatak detaljnog opisa radova te krivo tumačenje mjera/neupućenost izvođača radova. Planira se nastavak suradnje ključnih sektora, edukacija dionika, odobravanje izvođenja samo nužnih radova, bolji sustav nadzora i izvješćivanja, monitoring učinkovitosti mjera te njihovo unapređivanje u skladu s rezultatima, daljnja razrada mjera zaštite te provođenje ocjene prihvatljivosti na strateškoj razini.
Ključne riječi: uvjeti i mjere zaštite prirode, Program radova održavanja voda, ocjena prihvatljivosti ASSESSMENT OF THE PROGRAMME FOR MAINTENANCE WORKS FOR PROTECTION AGAINST HARMFUL EFFECTS OF WATER IN CROATIA – NATURE PROTECTION CONDITIONS AND MEASURES: FUNDAMENTALS, CHALLENGES AND PLANS FOR THE FUTURE M. Sabolić1, T. Čuković2, A. Duplić1, P. Lunko1, D. Schneider1 1 Department for nature impact assessment, State Institute for Nature Protection, Zagreb, Croatia ([email protected]) 2 Paljetkova ulica 2, Zagreb, Croatia Maintenance works affect the hydromorphological and biological elements of watercourses which can adversely affect their ecological status. Around 32,100 km of natural and artificial watercourses in Croatia is managed by state's water agency Hrvatske vode. The Nature Protection Act stipulates that natural resources management plans include nature protection conditions, and appropriate assessment is conducted for those which can have a significant impact on conservation objectives and integrity of the ecological network. Cooperation of water and nature protection sectors began in 2011 with dividing works into 11 groups, for which SINP has developed 70 standardised protection measures, based on a good practice examples from the literature. Issued measures refer to the overall biodiversity and conservation objectives of the ecological network. Regarding ecological needs of target species th
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specific measures were devised. Since 2012 problems in assessing the Programme are late submission which can lead to the beginning of works before conditions are issued, deficient works description and misinterpretation of measures/uninformed contractors. Plans for the future are further cooperation between key sectors, stakeholder education, realization of only necessary works, better system of monitoring and reporting, monitoring efficiency of measures and their improvement based on the results, further elaboration of measures and appropriate assessment at the strategic level. Key words: nature protection conditions and measures, Programme for water maintenance works, appropriate assessment P‐20 DRVENE OBALOUTVRDE: EKOLOŠKA ALTERNATIVA KAMENU I BETONU NA OBALAMA VODOTOKA R. Skukan, N. Koletić, L. Derežanin, N. Malešević, R. Španić, S. Dekanić Institut za istraživanje i razvoj održivih ekosustava, Velika Gorica, Hrvatska ([email protected]) Održivo upravljanje vodama u skladu s Okvirnom direktivom o vodama uključuje ekološke, hidrotehničke i upravljačke mjere i postupke koji su usmjereni prema zadržavanju ili obnovi prirodnog stanja vodotoka. Erozija obale predstavlja jedan od ključnih prirodnih procesa dinamike vodotoka kojim je u određenim slučajevima potrebno upravljati. Ovaj rad za cilj ima prikazati mogućnost uporabe drvenih obaloutvrda kao ekološki prihvatljive hidrotehničke mjere za stabilizaciju obala vodotoka u svrhu upravljanja procesom erozije. Za gradnju drvenih obaloutvrda koriste se stabla tvrdih listača koja su uzgojena u rijetkim nasadima. Sidrenjem stabala duž obale potiče se prirodno taloženje nanosa unutar grana krošanja te se usporava tok vode i smanjuje erozija obale. Nataloženi riječni nanos unutar i izvan krošanja pruža vlažnu i plodnu podlogu za razvoj vegetacije i mjesta za skrovišta divljim životinjama. S vremenom drveni dijelovi obaloutvrde trule, a na lokaciji ostaje posađena autohtona obalna vegetacija koja sprječava daljnje erodiranje obale. Za razliku od klasičnih obaloutvrda koje se grade od kamenog nabačaja i materijala, drvene obaloutvrde uz kontrolu erozije obale omogućuju i zadržavanje ili obnovu prirodi bliskog obalnog staništa. Drvene bi obaloutvrde trebale imati prednost nad kamenim i betonskim konstrukcijama u nedirnutim dijelovima vodotoka, u zahvatima revitalizacije antropogeno izmijenjenih vodotoka te posebice u upravljanju vodotocima u zaštićenim područjima prirode. Ključne riječi: drvene obaloutvrde, upravljanje vodama, erozija obala, ekološke metode stabilizacije obala TREE REVETMENTS: ECOLOGICAL ALTERNATIVE TO STONE AND CONCRETE AT THE RIVER BANK R. Skukan, N. Koletić, L. Derežanin, N. Malešević, R. Španić, S. Dekanić Institute for research and development of sustainable ecosystems, Velika Gorica, Croatia ([email protected]) Sustainable water management in accordance with the Water Framework Directive includes environmental, hydro‐technical and control measures designed to maintain or reintroducenatural features of watercourses. One of the key processes in the riverine natural dynamics, that requires active management, is erosion of river banks. In this paper we aim to demonstrate the possibility th
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of using wood revetmentsas anenvironmentally‐friendly methodto stabilize stream banks and control erosion. Hardwood trees grown in plantations are commonly used for the construction of tree revetments. By anchoring the trees along the river banks, natural deposition of sediment within the branches of the tree crowns is encouragedwhile slowing the flow of water in the same time, thereby reducing the overall effect of erosion. Moreover, precipitated river deposit among canopies provides a moist and fertile basis for the development of vegetation and shelter for wild animals. Finally, woodrots and planted native riparianvegetation remains to prevent further erosion. In contrast to conventional revetments that are built from stone and concrete, tree revetments control erosion and allow the maintenance or revitalization of riparian habitats. Tree revetments have clear advantage over the stone and concrete in pristine natural parts of the watercourses, for the revitalization of anthropologically altered watercourses and especially in the management of watercourses in protected areas. Key words: tree revetments, watercourse management, coastal erosion, ecological methods of river banks stabilization 2. SIMPOZIJ EDUKACIJE BIOLOGIJE 2nd BIOLOGY EDUCATION SYMPOSIUM P‐ 21 PRIMJENA ONLINE TEČAJA ZA UČENJE BIOLOGIJE U SREDNJOJ ŠKOLI S. Fabac1, V. Fabijanić2 1 Gimnazija Vladimira Nazora, Zadar, Hrvatska ([email protected]) 2 Srednja škola Bartula Kašića, Pag, Hrvatska Online alati se sve više primjenjuju u nastavnom procesu. E‐obrazovanje postaje tehnološki imperativ, a uvode se nove metode učenja koje će učenike pratiti kroz daljnje školovanje. Online tečajevi uključuju moderne elemente učenja i poučavanja u odnosu na tradicionalnu nastavu. Učenik uči po vlastitom tempu, u vrijeme koje mu odgovara. Pretpostavka je da primjena online tečaja doprinosi boljem razumijevanju i povećanom interesu za nastavne sadržaje iz biologije. Pomoću online tečajeva uspostavlja se bolja komunikacija među učenicima preko razmjene iskustava i ideja na forumima. Također se uspostavlja i bolja komunikacija učenika s nastavnicima kroz razmjenu pitanja i odgovora. Dodatno motivira i mogućnost vrednovanja znanja. U tečaju je omogućeno da nastavnici evaluiraju ishode učenja polaznika. Cilj istraživanja bio je usporediti rezultate učenika 1. i 3. razreda Gimnazije Vladimira Nazora iz Zadra i Srednje škole Bartula Kašića iz Paga, koji imaju podjednak uspjeh iz biologije. Učenici kontrolne skupine nisu koristili online alate, a učenici pokusne skupine bili su aktivni sudionici online tečaja. Provedena je i anketa s pitanjima zatvorenog tipa među učenicima pokusne skupine. Obrada podataka potvrdila je našu hipotezu: (1) da je povećan interes za sadržaje iz biologije i (2) da su postignuti bolji rezultati. Stoga možemo zaključiti da se korištenjem online tečaja kod učenja lakše postižu ciljevi i ishodi nastave biologije, te se unaprjeđuju kognitivna postignuća učenika. Ključne riječi: Online tečaj, ciljevi i ishodi, kognitivna postignuća, biologija APPLICATION OF ON‐LINE SEMINAR FOR STUDYING OF BIOLOGY IN HIGH SCHOOL S. Fabac1, V. Fabijanić2 th
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1
High School Vladimir Nazor, Zadar, Croatia ([email protected]) High School Bartul Kašić, Pag, Croatia
2
The online tools are being more frequently applied in education process. Our presumption is that the application of the online seminars contributes to a better understanding and to an increased interest for education alcontents of biology. Via on‐line seminars, it is established a better communication between pupils through exchange of experiences and ideas on the forums. Also, a better communication is established between pupils and teachers through exchange of questions and answers. The aim of this investigation was to compare the results of learning between pupils of the 1st and 3rd class of the High School Vladimir Nazor, Zadar and those from the High School Bartul Kašić, Pag, which have attained almost the same success in learning biology. The pupils from the control group did not use the online tools while the pupils from the experimental group actively participated in the online seminar. We have also performed the questionnaire including of the questions of the 'closed type' (blind type) among the pupils from the experimental group. Data analysis confirmed our hypothesis that the online learning resulted in: (1) an increased interest for biology contents and (2) better results of learning. According to that, we concluded that the use of the online seminar for learning may facilitate the attaining of objectives and outcomes in the education of biology and may improve the cognitive accomplishments of pupils. Keywords: online seminar, objectives and outcomes, cognitive accomplishments, biology P‐22 USVOJENOST OSNOVNIH KONCEPATA IZ BOTANIKE KOD UČENIKA PETOG RAZREDA OSNOVNE ŠKOLE N. Kletečki1, D. Novoselić2 1 OŠ Bogumila Tonija, Samobor, Hrvatska ([email protected]) 2 Alfa d.d., Zagreb, Hrvatska Cilj istraživanja bio je utvrditi usvojenost osnovnih koncepata botanike koje su učenici usvojili na kraju prvog obrazovnog ciklusa iz nastavnog predmeta „Priroda i društvo“ u nižim razredima osnovne škole. Istraživanje je provedeno s učenicima 5. razreda osnovne škole, a prije obrade nastavne teme 'Biljka cvjetnjača' nastavnog predmeta 'Priroda'. Instrument istraživanja sastojao se od niza zadataka objektivnog tipa. Sadržavao je ukupno 26 pitanja koja su ispitivala tri kognitivne razine znanja: reproduktivno znanje (prva razina), konceptualno razumijevanje (druga razina) te rješavanje problema (treća razina). Zadaci su bili otvorenog tipa (nadopunjavanja, dosjećanja i esejskog tipa) te zatvorenog tipa (alternativnog izbora, višestrukog izbora, povezivanja ujednačenog tipa). Utvrđena je nedovoljna usvojenost osnovnih koncepata botanike. Učenici bolje rješavaju zadatke kojima se ispituje reproduktivno znanje, a manje su uspješni u rješavanju zadataka kojima se ispituju više kognitivne razine znanja. Ključne riječi: priroda, botanika, niži razredi osnovne škole, koncepti, kognitivne razine ACQUISITION LEVEL OF BASIC BOTANY CONCEPTS BY ELEMENTARY SCHOOL STUDENTS IN THE FIFTH GRADE N. Kletečki1, D. Novoselić2 th
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1
Bogumil Toni Elementary School, Samobor, Croatia ([email protected]) Alfa d.d., Zagreb, Croatia
2
The aim of this research was to determine the acquisition level of basic botany concepts that students adopted by the end of the first educational cycle in the subject 'Nature and Society' during the first four years of their formal education. Research was conducted among elementary school students in the fifth grade, in Nature Class, before the chapter 'The Flowering Plant'. The survey instrument consisted of 26 objective test items that assessed three levels of cognitive domain: recalling knowledge (first level), conceptual understanding (second level) and problem solving (third level). Tasks were open‐ended (short answers, recalling facts and essays) and fixed‐choice (alternative choice, multiple choice, matching). Results show an unsatisfactory permanence of knowledge of basic botany concepts. Students were more successful at solving tasks that tested knowledge, and less successful at solving tasks that required higher thinking skills. Keywords: nature, botany, primary school, elementary school, concepts, cognitive domain P‐23 USVOJENOST NASTAVNOG SADRŽAJA PRIMJENOM KONCEPTUALNIH MAPA KOD UČENIKA SREDNJE ŠKOLE K. Latin1, E. Merdić2, M. Ništ3, I. Labak2 1 Osnovna škola Vladimir Nazor , Čepin, Hrvatska ([email protected]) 2 Odjel za biologiju, Osijek, Hrvatska 3 Agencija za odgoj i obrazovanje, Osijek, Hrvatska Cilj istraživanja bio je usporediti nivo usvojenog znanja stečenog primjenom konceptualnih mapa s nivoom usvojenog znanja stečenog tradicionalnim (frontalnim) oblikom rada. Također se ispitao i nivo retencije znanja. U istraživanje su bili uključeni učenici drugih razreda Prirodoslovno‐matematičke gimnazije u Osijeku (101 učenik). Tijekom travnja i svibnja školske godine 2013./2014. provedeni su nastavni sati s obradom nastavnih tema 'Ptice' i 'Sisavci'. Nastavne teme u dva su razreda obrađene frontalnim oblikom rada, a u dva razreda pomoću konceptualnih mapa nakon čega je uslijedilo testiranje učenika pisanom provjerom znanja. U rujnu sljedeće školske godine (2014./2015.) proveden je test retencije znanja sa svim učenicima. Istraživanjem je utvrđeno da učenici koji su u učenju koristili konceptualnu mapu postižu statistički značajno bolje rezultate u odnosu na učenike koji su sadržaj učili frontalnim oblikom rada. Isti rezultat je dobiven i nakon provedenog testa retencije. Ovim radom prikazana je mogućnost primjene konceptualnih mapa u učenju i poučavanju sadržaja iz Biologije kao efikasne nastavne metode koja pridonosi postizanju konceptualnog razumijevanja. Ključne riječi: konceptualna mapa, retencija znanja, biologija ADOPTION OF TEACHING CONTENT USING CONCEPTUAL MAPS AMONG SECONDARY SCHOOL STUDENTS K. Latin1, E. Merdić2, M. Ništ3, I. Labak2 1 Primary School Vladimir Nazor , Čepin, Croatia ([email protected]) 2 The Department of Biology, the University of Osijek, Osijek, Croatia th
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3
Education and Teacher Training Agency, Osijek, Croatia
The aim of this research was to explore whether the students achieve better results during their learning process using conceptual maps or expository teaching. Also, the level of knowledge retention was tested.This research was conducted on the sample of 101 students of second grade of Natural Sciences High School and Mathematics High School in Osijek. During April and May in the school year 2013‐2014 students have been teaching about two topics: 'Bird' and 'Mammals'. In two classes students used traditional way of learning, so‐called direct instruction. In other two classes student taught by themselves using conceptual maps. After teaching period was completed, all students wrote final test. Next school year (2014‐ 2015) in September the knowledge retention test was carried out with all students. This research showed that students who used conceptual maps during learning process achieve statistical significant better results compared to students who have teach by using direct instructions. The same result is obtained after the retention test. This paper presents the good possibility for applying the conceptual maps in learning and teaching biology topics as an effective teaching method that contributes to the achievement of conceptual understanding.
Keywords: conceptual map, retention of knowledge, biology
P‐24 UTJECAJ KOGNITIVNIH RAZINA ZADATAKA NA VJERODOSTOJNOST USVOJENOSTI KONCEPATA GENETIKE KOD GIMNAZIJSKIH UČENIKA
PROCJENE
M. Mihalić, H. Kostadinović Prirodoslovno‐matematički fakultet, Zagreb, Hrvatska ([email protected])
Cilj ovog rada bio je istražiti kako na vjerodostojnost provjere usvojenosti nastavnih koncepata utječe struktura zadataka postavljenih u tu svrhu. Analiziran je 141 test, što predstavlja 10% najbolje riješenih testova sa županijskog natjecanja iz biologije za IV. razred gimnazije, provedenog 2014. godine, na području cijele Hrvatske. Izdvojena su tri zadatka čija je riješenost bila najlošija, a koji su obuhvaćeni nastavnom cjelinom genetika. Utvrđeno je koje koncepte ispituju navedeni zadaci, a to su: 1) Kodiranje za aminokiseline; 2) Kretanje alela u populaciji; 3) Nastanak rekombinantnih potomaka. Zatim su sastavljeni novi testovi koje je činilo ukupno devet zadataka. Ti zadaci su postavljeni tako da se pomoću njih provjerava usvojenost utvrđenih koncepata na sve tri razine znanja (svaki od tri koncepta ispitan je s tri nova pitanja, svako na jednoj od tri razine znanja). Novi testovi su dani na riješavanje učenicima četvrtih razreda V. i XV. gimnazije u Zagrebu (ukupno 54 učenika). Iz dobivenih rezultata može se izvući zaključak da vjerodostojnost procjene usvojenosti koncepata dobrim dijelom ovisi o pristupu ispitivača u oblikovanju zadataka. Isto tako možemo zaključiti da riješenost zadataka viših kognitivnih razina ne mora biti garancija za potpunu usvojenost koncepta i ostvarenje planiranih ishoda učenja na nižim razinama. Da se ostvari što sigurnija procjena usvojenosti određenog koncepta od strane učenika, poželjno je ispitati taj koncept na sve tri razine znanja. Ključne riječi: kognitivna razina, koncept, zadatak IMPACT OF COGNITIVE LEVELS OF TASKS ON GENETICS CONCEPTS ASSESSMENT CREDIBILITY IN HIGH SCHOOL STUDENTS
M. Mihalić, H. Kostadinović Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) th
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The aim of this study was to investigate how the structure of tasks affects concepts assessment credibility. 141 tests were analyzed, which represents 10% of the best solved tests in Croatian National Biology Competition for 12th grade (4. high school grade) students, conducted in 2014. Three worst solved tasks from genetics topic were observed. Concepts that evaluate those tasks were determined, and they are: 1) Amino acid coding; 2) Movement of alleles in a population; 3) Recombinant offspring production. New tests with nine tasks were prepared. These tasks were used to evaluate adoption of the established concepts at all three cognitive levels of knowledge (each of the three concepts was evaluated with three new tasks, each task on one of the three cognitive levels). New tests were given to 12th year students of V. Gimnazija and XV. Gimnazija in Zagreb (54 students total). It can be concluded from the results that concept assessment credibility depends on structure of tasks. We can also conclude that solving of tasks on higher cognitive levels may not be a guarantee for realization of intended learning outcomes at lower cognitive levels. It is desirable to examine the concept at all three cognitive levels of knowledge, to make sure students have adopted the concepts properly.
Keywords: cognitive level, concept, task
P‐25 KORIŠTENJE LOKALNIH RESURSA U TERENSKOJ NASTAVI BIOLOGIJE M. Rašan1, S. Mikulić2, D. Sumpor3, M. Labaš Horvat1 1 Srednja škola Prelog, Prelog, Hrvatska ([email protected]) 2 Gimnazija Fran Galović, Koprivnica, Hrvatska 3 Srednja škola Tina Ujevića, Kutina, Hrvatska U Srednjoj školi Prelog, Gimnaziji Fran Galović i Srednjoj školi Tina Ujevića je u okviru izborne nastave biologije u drugim i trećim razredima gimnazije zastupljen terenski rad. Nastava ekologije, botanike i zoologije na terenu je vezana uz istraživačke učeničke projekte. Za učenička istraživanja živog svijeta korišteni su prirodni ekosustavi. Cilj rada je ispitati stavove učenika o kompetencijama koje steknu tijekom vlastitog istraživanja koristeći ekosustave svog okruženja. Kontrolnu skupinu čine učenici drugih i trećih razreda gimnazije koji nisu radili terensko istraživanje, a iz biologije su ocjenjeni ocjenom vrlo dobar ili odličan. Eksperimentalnu skupinu čine učenici drugih i trećih razreda navedenih škola koji su radili terensko istraživanje. Pretpostavka je da će učenici koji su radili terenska istraživanja cjelovitije, svrsishodnije sagledavati i objasnjavati uzročno‐posljedične veze i povezivati s kognitivnim postignućima te znati povezati sa primjenom u svakodnevnom životu. Za istraživanje je korištena anketa u kojoj su ispitanici odgovarali na pitanja zatvorenog tipa brojčano prema skali po Likertu (1‐ uopće ne; 2‐ uglavnom ne; 3‐ nisam siguran; 4‐ uglavnom da; 5‐ u potpunosti). Obradom rezultata ankete ustanovljeno je da je eksperimentalna skupina izrazila pozitivnije statove prema uspjesima baziranih na korištenju lokalnih prirodnih resursa u učeničkim istraživačkim projektima. Nastava prirodnih znanosti trebala bi se više usredotočiti na metodu istraživačkog rada koristeći lokalne prirodne resurse. Kvalitenije usvojeni koncept održivog razvoja je temelj za aktivnije uključivanje u rješavanje problema u lokalnoj zajednici. Učenici eksperimentalne grupe smatraju da je rad na istraživačkom projektu poboljšao razvoj njihovih govorničkih vještina. Pri provjeri znanja o ekosustavima koji ne pripadaju lokalnom prirodnom okolišu učenici eksperimentalne skupine su pokazali bolje rezultate.
Ključne riječi: učenički istraživački projekt, terenski rad, kompetencije th
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USING LOCAL RESOURCES IN BIOLOGY FIELD WORK
M. Rašan1, S. Mikulić2, D. Sumpor3, M. Labaš Horvat1 1 High School Prelog, Prelog, Croatia ([email protected]) 2 High School Fran Galović, Koprivnica, Croatia 3 High School Tina Ujevića, Kutina, Croatia
In Secondary school Prelog, Grammar school Fran Galović and Secondary school Tin Ujević field work is a part of elective Biology classes for students in the second and third grade. Teaching ecology, botany and zoology in the field is connected to student research projects. For the student research of the living world, natural ecosystems have been used. The topic of the paper is to examine student attitudes towards competencies that they acquire during their individula research while using ecosystems in their surroundings. The control group is made up of the second and third year grammar school students who have never done field research and have an A or B in Biology. The experimental group is made up of the second and third year students from above mentioned schools who have done field research. The assumpion is that the students who have done field work will look at problems more thoroughly and with more sense and will be able to explain cause and effect relationships, connect it to cognitive achievements and will know how to connect it to every‐day situations. For the sake of research, a poll was used where the examinees answered closed type questions on the Likert scale (1‐ not at all; 2‐ mostly no; 3‐ not sure; 4‐ mostly yes; 5‐ completely). After data processing, the results showed that the experimental group has stated more positive attitudes towards accomplishments based on usage of local natural resouces in student research projects. Science classes should focus more on the method of research study, using local natural resources leads to a quality understanding of sustainable development which leads to more active involvement in the political life of a grown‐up responsible citizen. Experimental group students think that working on a research project has lead to development of independence and security in presenting. Experimental group of students has shown better results during tests when examined about ecosystems which do not belong to local environment. Key words: students' research projects, field research, competencies P‐26 IGRA U PONAVLJANJU NASTAVNIH SADRŽAJA VEZANIH UZ ŽIVOTNE ZAJEDNICE MORA J. Razlog‐Grlica1, N. Kletečki2, M. Novosel3 1 OŠ Ivane Brlić‐Mažuranić, Virovitica, Hrvatska (jasna.razlog‐[email protected]‐com.hr) 2 OŠ Bogumila Tonija, Samobor, Hrvatska 3 Prirodoslovno‐matematički fakultet, Biološki odsjek, Zagreb, Hrvatska U nastavi prirode 6. razreda obrađuju se teme vezane uz more u nastavnoj cjelini 'Životne zajednice mora i voda na kopnu'. Kod ponavljanja sadržaja iz ovog područja učiteljima je teško postići maksimalnu učeničku koncentraciju i aktivnost svih učenika, pogotovo ako se sat svede na klasično propitivanje. Upravo zato se umjesto klasičnih metoda ponavljanja uvodi igra. Cilj je unaprjeđivanje kvalitete nastavnog procesa i uspostavljanje aktivnijeg sudjelovanja učenika u usvajanju nastavnih sadržaja vezanih uz more kao stanište u 6. razredu. Tijekom provođenja ponavljanja gradiva primjenjuju se igre asocijacije, križaljke i rebusi, koje učenici rješavaju u grupama. Uspoređuju se i rezultati anketiranja po 30 učenika 6. razreda iz OŠ Bogumila Tonija th
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Samobor i OŠ Ivane Brlić‐Mažuranić Virovitica, čime se ispituje motiviranost učenika i njihovo zadovoljstvo ovakvim načinom rada. Učenici OŠ Bogumila Tonija Samobor su tijekom obrade nastavnih sadržaja vezanih uz more koristili zbirku morskih organizama i edukacijsku knjižicu 'More je živo' Društva za ekološka istraživanja Paks s popratnim video materijalima dok su učenici OŠ Ivane Brlić‐Mažuranić Virovitica koristili samo zbirku morskih organizama.Vrijednost primjene igre u nastavi prirode je u tome što se kod učenika, razvija osjećaj zadovoljstva usvojenim znanjem uz pozitivan natjecateljski duh kojim se potiče usmena komunikacija, tolerancija, uči slušati i jača samopuzdanje učenika. Na ovaj način učenici brže i lakše usvajaju sadržaje i uče se timskom radu. Ključne riječi: ponavljanje, igra, osnovna škola, životne zajednice mora THE PLAY IN REPEATING THE EDUCATIONAL CONTENTS ABOUT MARINE LIFE J. Razlog‐Grlica1, N. Kletečki2, M. Novosel3 1 'Ivana Brlić‐Mažuranić' Primary school, Virovitica, Croatia (jasna.razlog‐[email protected]‐com.hr) 2 'Bogumil Toni' Primary school, Samobor, Croatia 3 Faculty of Science, Biological Department, Zagreb, Croatia In primary schools topics about marine life are elaborated in the 6th grade nature education, in the educational unit 'Marine and freshwater life communities'. It is difficult for teachers to achieve maximum pupils' concentration and activity while repeating the contents of this topic, especially if the class is done in a way of classical knowledge testing. It is for this reason that the play is introduced into the learning process. The goal is to improve the educational process and to establish a more active participation of pupils in acquiring the educational contents related to marine habitats in the 6th grade. In repeating of these contents, games of association, crosswords, dice and rebus are introduced into the learning process. Results of a poll taken among 30 pupils of the 6th grade from the 'Bogumil Toni' Primary school in Samobor and 30 pupils of the 6th grade from the 'Ivana Brlić‐Mažuranić' Primary school in Virovitica are compared, examining their motivation and their satisfaction with this way of learning. Pupils from the 'Bogumil Toni' Primary school in Samobor in their classes about marine life also used a collection of sea organisms and an educational booklet 'The sea is alive' published by the Society for ecological research Paks with accompanying video material, while pupils from the 'Ivana Brlić‐Mažuranić' Primary school in Virovitica used only a collection of sea organisms. The value of play in teaching nature contents lies in the fact that pupils develop a capability of oral communication and tolerance, learn to listen, strengthen their self‐confidence, develop a sense of satisfaction with the acquired knowledge and encourage their competitive skills. In this way pupils learn faster and more easily acquire educational contents, as well as learn how to work in team. Keywords: repeating, play, primary school, marine life P‐27 EKOLOŠKE TEME U UDŽBENICIMA REPUBLIKE HRVATSKE I SAVEZNE REPUBLIKE NJEMAČKE Š. Romštajn‐Burchards, I. Bogut, Ž. Popović Fakultet za odgojne i obrazovne znanosti, Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek ([email protected]) th
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Europska obrazovna politika usmjerena na razvoj kompetencija učenika naglašava važnost integracije ekoloških kompetencija u nacionalne okvirne kurikule. Kompetencije učenika za područje ekologije obuhvaćaju poznavanje svijeta prirode, zakonitosti održivog razvoja, kritičko poimanje znanosti i tehnologije po pitanju etike, sigurnosti i zaštite okoliša i dr. U radu su analizirani sadržaji Nacionalnih obrazovnih kurikula (NOK i NRW), npr. kompetencije učenika nižih razreda osnovne škole u svezi ekološkog odgoja, te udžbenici Prirode i društva s ciljem usporedbe zastupljenost ekoloških tema u njima u Republici Hrvatskoj i u Saveznoj Republici Njemačkoj, pokrajini Nordrhein‐Westfallen. Može se zaključiti kako su u oba kurikula vrlo dobro zastupljene kompetencije učenika koje odražavaju ekološku pismenost. Rezultati kvantitativne analize govore da se u hrvatskim udžbenicima iz prirode i društva 1.‐ 4. razreda nalazi najviše šest ekoloških tema, a u njemačkim njih dvadeset. Utvrđene su i raspravljane razlike u pristupu pojedinim temama. Pretpostavlja se kako je holistički pristup ekološkoj tematici u Nacionalnom kurikulu Njemačke i njihovim udžbenicima jednim dijelom posljedica okolišne politike Europske Unije koja promiče ekološki standard temeljen na akcijskim programima. Uspjeh se ekološkog odgoja najbolje mjeri ponašanjem čovjeka pa je zato važno osigurati uvjete u kojima učenici svakodnevno mogu razvijati svoje ekološke kompetencije doprinoseći tako što boljoj sutrašnjici. Ključne riječi: Nacionalni obrazovni kurikul, Hrvatska i Njemačka, udžbenici, okoliš, priroda i društvo ENVIRONMENTAL ISSUES IN TEXTBOOKS OF CROATIAN AND FEDERAL REPUBLIC OF GERMANY Š. Romštajn‐Burchards, I. Bogut, Ž. Popović Faculty of Education, Josip Juraj Strossmayer University of Osijek, Osijek ([email protected]) European educational policy focused on developing competencies of students emphasizes the importance of integration of environmental competencies in national framework curricula. Competences of students in the field of environmental issues include understanding world of nature, the principles of sustainable development, a critical understanding of science and technology in terms of ethics, safety and environmental protection etc. The paper analyzes contents of the national educational curricula (NOK and NRW), e.g.: competences of lower grade primary school students on environmental issues, and analyzes textbooks for Nature and Society in Croatia and Germany (Nordrhein‐Westfalen province) in order to compare presence of environmental topics in them. It can be concluded that in both curricula pupils’ competencies reflecting environmental literacy are very well represented. Results of quantitative analysis show that the Croatian Nature and Society textbooks 1st‐4th grade is up to six environmental topics, contrary to twenty in German textbooks. Some observed differences in the approach to particular topics were discussed as well. We assume that a holistic approach to environmental issues in the National Curriculum of Germany and their textbooks, are partly a consequence of the environmental policy of the EU which promotes standards based on action programs. It is important to provide conditions in which pupils can develop their daily environmental abilities. Keywords: National education curriculum, Croatia and Germany, textbooks, environment, nature and society P‐28 UČENIČKO POZNAVANJE ŽIVOTINJA HRVATSKE M. Srkoč1, I. Štefančić1, R. Halapir Franković2 th
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1
Prirodoslovno matematički fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska ([email protected]) 2 V. gimnazija, Zagreb, Hrvatska Cilj istraživanja bio je analizirati koliko učenici poznaju činjenice o životinjama koje žive u Hrvatskoj te je postavljena hipoteza da poznavanje životinja ovisi o nastavnom programu, pri čemu će najbolje rezultate ostvariti učenici 3. razreda. Provedeno je 2013. godine na uzorku od 127 učenika od 1. do 4. razreda V. gimnazije u Zagrebu.Učenici su rješavali anketu s 2 tipa pitanja. U prva 3 pitanja traženo je opće znanje o životinjama Hrvatske, a u sljedećih 16 učenici su trebali zaokružiti jesu li navedene tvrdnje o životinjama točne ili netočne. Pitanja su podijeljena u kategorije: a) jesu li ili nisu u nastavnom planu i programu, b) na ona koja jesu ili nisu važna za život učenika, c) pitanja vezana uz raznolikost te d) pitanja vezana uz prilagodbe životinja.Na temelju dobivenih podataka može se uočiti da su najslabije rezultate postigli učenici 1. razreda, a najbolje učenici 3. razreda. Nisu uočene razlike s obzirom na spol te se može zaključiti da djevojčice i dječaci podjednako poznaju životinje Hrvatske. Ukupna riješenost od oko 60% upućuje na zaključak da bi se u nastavi biologije trebalo više pažnje posvetiti toj tematici kako bi učenici shvatili važnost upoznavanja određenih vrsta i njihovih karakteristika što zbog životne važnosti (prepoznavanje zmija otrovnica) što zbog opće kulture (autohtone pasmine Hrvatske). Ključne riječi: istraživanje, V. gimnazija, životinje Hrvatske, analiza anketa STUDENT`S KNOWLEDGE OF ANIMALS THAT LIVE IN CROATIA M. Srkoč1, I. Štefančić1, R. Halapir Franković2 1 Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) 2 Fifth Gymnasium, Zagreb, Croatia The aim of this study was to analyze how much facts the students know about the animals that live in Croatia and it has been hypothesized that knowledge of animals depends on the curriculum. The best results achieved 3rd grade students. The study was made in 2013 on a sample of 127 students into 4 grades in V. High School in Zagreb. Students were solving 2 types of survey questions. In the first three questions they were asked about general knowledge of animals in Croatia, and in the following 16, students had to choose whether the following statements about animals are correct or incorrect. The questions are divided into categories: a) to those that are or are not in the curriculum, b) to those that are or are not relevant to the life of students, c) questions related to diversity and d) questions related to the adaptation of animals.Based on the results, the lowest score had 1st grade students, and the best score had students of 3rd grade. There is no essential difference between the knowledge of girls and boys. The total score of about 60% indicates that biology teachers should pay more attention to this topic so that students understand the importance of getting to know certain species and their characteristics. Because that is very important for every student (for example, students should be able to identifying poisonous snakes). Keywords: study, V. High School, animals of Croatia, analisis of survey P‐29 STAVOVI UČITELJA O BIOLOŠKIM NASTAVNIM SADRŽAJIMA U OSNOVNOJ ŠKOLI A. Šarić, M. Marić th
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Prirodoslovno matematički fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska ([email protected]) Cilj istraživanja bio je utvrditi stavove učitelja Prirode i Biologije o Nastavnom planu i programu u osnovnoj školi. 180 učitelja u razdoblju od 2004. do 2006. godine izražavali su slaganje uz 18 pitanja anketnog upitnika vezanih za nastavni program Prirode i Biologije. Nastavni plan i program itekako utječe na učenička postignuća te na njihove interese i stavove prema predmetu (citat). 84% učitelja se izjasnilo da se zadaće u Nastavnom planu i programu temelje na činjenicama.46% učitelja smatra da se novi koncepti ne izgrađuju prema usvojenim predkoncepcijama. Učitelji su se također izjasnili da učenici ne razumiju gradivo o razmnožavanju u 5 razredu te njih 67% smatra da učenici to gradivo ne mogu povezati sa stvarnim životom i razvojem stava prema vlastitom zdravlju. Većina učitelja, njih 73% smatra da bi trebalo promijeniti postojeći Nastavni plan i program. Sadašnji Nastavni plan i program nije u dovoljnoj mjeri prilagođen dobi i interesima učenika te ne podupire izgradnju novih koncepata na usvojenim predkoncepcijama. Sadržaji Nastavnog programa bi trebali podupirati usvajanje prirodoznanstvene metode i kritički pogled na svijet na principu aktivnog učenja, što veći broj profesora u anketi smatra da nije zastupljeno u sadašnjem Nastavnom programu. Ključne riječi: kurikulum, učitelji, interesi, učenici TEACHERS' ATTITUDES TOWARD THE BIOLOGY CURRICULUM CONTENTS IN ELEMENTARY SCHOOLS A. Šarić, M. Marić Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) The aim of this study was determing attitudes of elementary school teachers toward the biology curriculum. From 2004 to 2006, 180 elementary school biology teachers took part in this study. They answered to a yes/no questionnaire, which contains 18 questions related to the biology curriculum. The curriculum has a major influence on students' achievements and their interests and attitudes toward the school subject (quote). 84% of teachers stated that the homework tasks in the curriculum are based on solely theoretical knowledge. 46% of teachers reported that students often do not use theoretical knowledge for acquisition of new knowledge. In addition, students in 5th grade do not comprehend the lessions about human reproduction, therefore they can not apply this knowledge in real life and do not see the benefits of it. 73% of teachers considered that the existing curriculum should be changed. The current curriculum is not sufficiently arranged to the age and interests of the students and does not encourage the developement of new knowledge. The content of the curriculum should support studying a natural‐scientific method, have a critical view and encourage active learning. Many teachers in this survey agree with methods not being represented in the current curriculum.
Keywords: curriculum, students, biology, teachers EVOLUCIJA, SISTEMATIKA, FILOGENIJA I BIOGEOGRAFIJA EVOLUTION, SYSTEMATICS, PHYLOGENY AND BIOGEOGRAPHY
P‐30 THE POTENTIAL ROLE OF ECOLOGICAL ADAPTATION AND Wolbachia INFECTION IN THE DIVERSIFICATION OF Rhagoletis cerasi th
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V. Baković1, M. Schebeck1, C. Stauffer1, G. Ragland2, H. Schuler3 1 Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences, Vienna, Austria ([email protected]) 2 Department of Entomology, Kansas State University, USA 3 Department of Biological Sciences, University of Notre Dame, USA
Ecological adaptation to different hosts can be an important factor in the diversification of different species. The genus Rhagoletis is a model for sympatric speciation (speciation with gene flow). The apple maggot, Rhagoletis pomonella, has proved to be a prominent model case for sympatric speciation, due to adaptation to different hosts. Host choice is positively related to mate choice which results in prezygotic isolation. Similarly, the European cherry fruit fly Rhagoletis cerasi infests two different hosts, Prunus spp and Lonicera spp. The phenological and behavioural adaptation to the different hosts could lead to reduction of gene flow and potentially drive speciation. A previous study documented genetic differences between the different host populations for a single allozyme locus. To perform a more thorough genome wide search for differentiation we will use ddRADSeq, to identify genetic markers of host differentiation within sympatric and allopatric populations of R. cerasi. Additionally, previous studies showed that R. cerasi is infected by Wolbachia that causes cytoplasmic incompatibilities between host associated populations in allopatry. Therefore our ddRADSeq approach will allow us to determine the potential role of Wolbachia in the diversification of R. cerasi.
Key words: sympatric speciation, host‐induced differentiation, cytoplasmic incompatibility P‐31 PRIMJENA SUVREMENIH TEHNIKA PROSTORNE STATISTIKE U EKOLOŠKIM ISTRAŽIVANJIMA N. Bilić‐Dujmušić, B. K. Hackenberger Zavod za kvantitativnu ekologiju/Odjel za biologiju, Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska ([email protected])
Bitan dio kvantitativnog pristupa ekološkim istraživanjima je prostorna komponenta. Stoga su metode prostornog određenja, bez obzira radi li se o veličini areala vrste ili koncentraciji nutrijenata ili polutanata u nekom okolišnom kompartmentu, nezaobilazan korak u interpretaciji istraživanja. Razvojem informatičke znanosti paralelno s tehnologijom otvorile su se mogućnosti vrlo složenog simuliranja ekoloških eksperimenata što je dovelo do razvoja novih tehnika njihovog dizajniranja. Zahvaljujući suvremenim metodama prostorne statistike moguće je postići optimizaciju ekoloških istraživanja u smislu reduciranja potrebnog vremena, potrebne veličine uzoraka i veličine obuhvaćenog prostora. U ovom radu će biti prikazana primjena simulacije temeljene na prostorno statističkim modelima i simulacijama uzorkovanja faune tla. Usporedbom rezultata dobivenih simuliranim eksperimentom u laboratoriju i realnim eksperimentom u okolišu ustanovila se statistički značajna sličnost. U okviru rada donesene su hipoteze o porijeklu greške simuliranog i realnog rezultata. Ključne riječi: prostorna statistika, ekologija, fauna tla APPLICATION OF MODERN SPATIAL STATISTICAL TECHNIQUES IN ECOLOGICAL RESEARCH N. Bilić‐Dujmušić, B. K. Hackenberger Subdepartment of Quantitative Ecology/Department of Biology, University of Josip Juraj Strossmayer in Osijek, Osijek, Croatia ([email protected]) th
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Spatial component is an important part of quantitative approach to ecological research. Whether they’re used for areal size or nutrient (or pollutant) concentrations in the environmental compartment, methods based on spatial determination are the essential step in the interpretation of research. Development of informatics sciences alongside with technology opened a lot of possibilities for complex simulation of ecological experiments which then led to development of new design techniques. Thanks to modern methods of spatial statistics, it is possible to achieve the optimization of ecological research in the term of reducing needed time, sample size and size of the sampled area. In this paper the application of simulation based on spatial statistical models will be shown together with simulation of sampling soil fauna. Through comparison of results obtained from simulated experiment in laboratory and real experiment in environment, statistical significance was established. In the framework of this paper, hypotheses were adopted regarding the origin of error in results obtained from simulated and real experiment. Key words: spatial statistics, ecology, soil fauna P‐32 GENETSKA STRUKTURA BJELONOGOG RAKA (Austropotamobius pallipes KOMPLEKS) OTKRIVA MOGUĆU POVEZANOST IZMEĐU TRI KRŠKE RIJEKE TIJEKOM GEOLOŠKE PROŠLOSTI M. Jelić1, F. Grandjean2, M. Govedič3, G. Klobučar1, D. Guyonnet4, I. Maguire1 1 Sveučilište u Zagrebu, Prirodoslovno‐matematički fakultet, Biološki odsjek, Zoologijski zavod, Zagreb, Hrvatska ([email protected]) 2 Université de Poitiers, Laboratoire “Ecologie et Biologie des Interactions», équipe “Ecologie, Evolution, Symbiose”, Poitiers, Francuska 3 Centre for Cartography of Fauna and Flora, Ljubljana, Slovenija 4 Laboratoire Signalisation et Transports Ioniques Membranaires STIM ERL 7368, Poitiers, Francuska Provedeno je opsežno istraživanje populacijsko‐genetičkih i morfoloških obilježja bjelonogog raka u jadranskom slijevu. Ovdje prikazujemo rezultate koji se odnose na analize triju populacija (rijeke Rječina (RJE), Reka (REK) i Glinščica (GLI)) kod kojih je zabilježen neočekivani genetski uzorak. Unatoč sadašnjoj geografskoj izoliranosti tih triju populacija, prema rezultatima genetskih analiza mitohondrijskih i mikrosatelitnih biljega, one se svrstavaju u istu grupu. Dobivena genetska struktura mogla je nastati kao posljedica prenošenja posredovanog čovjekom, ili kao posljedica prirodnog širenja. Prirodno širenje je najvjerojatniji uzrok dobivene strukture iz nekoliko razloga; Malo je vjerojatno da bi se dobivene umjerene vrijednosti očekivane heterozigotnosti (He =0,34 u GLI, =0,36 u REK, =0,44 u RJE) pojavile u novo uspostavljenim populacijama nako n prenošenja posredovanog čovjekom. Nadalje, povezanost između Reke i Rječine je već otprije utvrđena na osnovu proučavanja rasprostranjenosti vrsta roda Niphargus. Zaključno, prema geografskom položaju i hidrologiji istraživanih rijeka, prirodno širenje se moglo dogoditi kao posljedica povezanosti izvorišnih dijelova tijekom geološke prošlosti ili zbog širenja rakova kroz podzemne vodene sustave. Zadnju mogućnost treba uzeti u obzir jer postoje brojni nalazi bjelonogog raka, i njegove sestrinske vrste, potočnog raka, vrlo duboko unutar špiljskih sustava. Ključne riječi: istočno‐jadranski sljevovi, Hrvatska, Slovenija, mitohondrijski biljezi, mikrosatelitni biljezi th
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GENETIC STRUCTURE OF THE WHITE‐CLAWED CRAYFISH (Austropotamobius pallipes COMPLEX) REVEALED POSSIBLE HISTORICAL CONNECTIONS BETWEEN THREE KARSTIC RIVERS M. Jelić1, F. Grandjean2, M. Govedič3, G. Klobučar1, D. Guyonnet4, I. Maguire1 1 University of Zagreb, Faculty of Science, Department of Biology, Division of Zoology, Zagreb, Croatia ([email protected]) 2 Université de Poitiers, Laboratoire “Ecologie et Biologie des Interactions», équipe “Ecologie, Evolution, Symbiose”, Poitiers, France 3 Centre for Cartography of Fauna and Flora, Ljubljana, Slovenia 4 Laboratoire Signalisation et Transports Ioniques Membranaires STIM ERL 7368, Poitiers, France The comprehensive research on population genetics and morphological characteristics of the white‐clawed crayfish from Adriatic Basin was conducted. We present results that include analyses of three populations (Rječina (RJE), Reka (REK), and Glinščica (GLI) rivers), in which an unexpected genetic pattern was observed. Despite the current geographical isolation of these three populations, results of genetic analyses grouped them into the same cluster. The obtained genetic structure could be a consequence of human‐mediated translocations, or a consequence of natural dispersal. Natural dispersal is the most probable cause of obtained structure for several reasons; Observed moderate values of expected heterozygosity (He =0.34 in GLI, =0.36 in REK, =0.44 in RJE) were not likely to appear in newly established populations after human‐mediated translocations. Further, the connection between the Reka and Rječina rivers was recognized based on the distribution of Niphargus species. Finally, according to geographical position and hydrology of examined rivers, natural dispersal could appear due to historical headwater river captures or due to dispersal of crayfish through underground water systems. The last possibility should be taken into consideration because it is not unusual to find the white‐clawed crayfish and its congeneric species, the stone crayfish, very deep inside cave systems. Key words: Eastern Adriatic Basins, Croatia, Slovenia, mitochondrial markers, microsatellite markers P‐33 FILOGENIJA I FILOGEOGRAFIJA RODA Elmis (COLEOPTERA, ELMIDAE) KRŠKIH TEKUĆICA JUGOISTOČNE EUROPE L. Jovović1, B. Bruvo Mađarić1, V. Mičetić Stanković2,3, M. A. Jäch4, M. Kučinić3 1 Zavod za molekularnu biologiju, Institut “Ruđer Bošković”, Bijenička 54, HR‐10000 Zagreb, Hrvatska ([email protected]) 2 Hrvatski prirodoslovni muzej, Zagreb, Hrvatska 3 Bološki odsjek, Zoologijski zavod, Prirodoslovno‐matematički fakultet, Zagreb, Hrvatska 4 Prirodoslovni muzej u Beču, Vienna, Austria Vodeni kornjaši iz porodice Elmidae su slabo istraženi na području jugoistočne Europe pa je malo podataka i o njihovim filogenetskim i filogeografskim odnosima. Tako su za ovo istraživanje sakupljeni predstavnici navedene porodice u Hrvatskoj, Bosni i Hercegovini i Makedoniji, dok su oni iz Austrije korišteni za usporedbu. Upotrebom standardnih genetičkih markera (ITS1, COI i COII) pokazali smo da su vrste Elmis aenea i E. rioloides sestrinske te zajedno najsrodnije vrsti E. bosnica. Također, sve su tri navedene vrste u srodstvu sa vrstom th
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E. maugetii maugetii. S druge strane, filogenetski položaj vrste E. obscura nije još razjašnjen. Kod vrsta E. aenea i E. rioloides sakupljenih na rijeci Cetini uočene su morfološke razlike u odnosu na jedinke istih vrsta u središnjem dijelu Europe. Stoga je glavni cilj ovog istraživanja analizirati može li se uočena morfološka razlika potvrditi i na molekularnoj razini. Uzorci vrste E. aenea ne pokazuju značajne molekularne razlike s obzirom na uočeni morfološki dimorfizam i lokalitete. Analiza uzoraka vrste E. rioloides s rijeke Cetine ukazuje na odvajanje od tipičnih predstavnika srednjoeuropske faune podupirući dosadašnji status potencijalne kompleks vrste. Metodom molekularnog datiranja izračunali smo da je do razdvajanja između grupa vrste E. rioloides iz Srednje Europe i s područja Cetine došlo u Pleistocenu što je u skladu s pretpostavkom o važnosti pleistocenskih refugija u specijacijskim procesima na području jugoistočne Europe. Ključne riječi: vodeni kornjaši, Elmidae, kompleks vrste, Balkan, pleistocen PHYLOGENY AND PHYLOGEOGRAPHY OF GENUS Elmis (COLEOPTERA, ELMIDAE) IN KARSTIC RUNNING WATERS IN SOUTHEASTERN EUROPE L. Jovović1, B. Bruvo Mađarić1, V. Mičetić Stanković2,3, M. A. Jäch4, M. Kučinić3 1 Department for Molecular Biology, Ruđer Bošković Institute, Zagreb, Croatia ([email protected]) 2 Croatian Natural History Museum, Zagreb, Croatia 3 Department of Biology, Division of Zoology, Faculty of Science, Zagreb, Croatia 4 Museum of Natural History in Vienna, Vienna, Austria Elmidae is a water beetles family poorly studied in southeastern Europe, so phylogenetic and phylogeographic data are very scarce. Representatives of the family were collected in various freshwater habitats in Croatia, Bosnia and Herzegovina and Macedonia, while Austrian representatives were used for needed comparisons. Using standard genetic markers (ITS1, COI and COII) we revealed sister group of Elmis aenea and E. rioloides together being related to E. bosnica. E. maugetii maugetii forms a separate branch, while position of E. obscura could not be affirmed with high support. In addition, E. aenea and E. rioloides collected in Cetina River showed morphological variations from Central European representatives. Therefore, the main aim of this study was to analyse whether the observed differences can be confirmed at the molecular level. E. aenea shows high molecular h omogeny across all sampled populations in respect to both morphological features and locations. On contraire, screening of E. rioloides specimens indicated the separation of population from the Cetina River from typical representatives of Central European fauna, supporting the hypothesis that this is a complex species. By molecular dating we calculated that E. rioloides separated from Central European representatives in the Pleistocene. This is consistent with the observation of the importance of Pleistocene refugia in speciation processes in the Southeastern Europe. Key words: water beetles, Elmidae, complex species, Balkan, Pleistocene P‐34 SIMBIOZA HIDRE I ALGE: KARAKTERIZACIJA ENDOSIMBIOTSKIH ZELENIH ALGI IZOLIRANIH IZ RAZLIČITIH SOJEVA ZELENIH HIDRI POMOĆU ELEKTRONSKE MIKROSKOPIJE, MORFOMETRIJSKE I ENZIMSKE ANALIZE th
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N. Kević1, S. Radić Brkanac2, P. Peharec Štefanić3, F. Faraguna4, M. Kalafatić1, G. Kovačević1, D. Franjević1 1 Zoologijski zavod, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) 2 Botanički zavod, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 3 Zavod za molekularnu biologiju, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 4 Fakultet kemijskog inženjerstva i tehnologije, Sveučilište u Zagrebu, Zagreb, Hrvatska Proces endosimbioze jedan je od pokretača specijacije. Zelena hidra (Hydra viridissima Pallas, 1766) tipičan je primjer endosimbioze, jer u svojim gastrodermalnim mioepitelnim stanicama sadrži jednostanične fotoautotrofne alge. Endosimbiotske alge izolirane iz zelenih hidri održavamo u trajnim stabilnim laboratorijskim kulturama. Sojevi zelenih hidri prikupljeni su sa šest različitih geografskih lokaliteta: s četiri različite lokacije u Hrvatskoj, te iz Izraela i iz Njemačke. Endosimbiotske zelene alge izolirane su koristeći mikrobiološke metode. Izolirane endosimbiotske alge karakterizirane su prema citološko‐morfometrijskim parametrima te prema aktivnosti i sastavu enzima katalaze, peroksidaze i esteraza. Stanice endosimbiotskih algi karakterizirane su morfometrijskim mjerenjem promjera, opsega i površine. Alge HV i M9 odvojile su se od T i BV u veličini stanica. Rezultati dobiveni analizom sastava izoenzima upućuju na razliku između algalnih endosimbionata s hrvatskih lokaliteta i algalnih endosimbionata iz Europe, ukazujući na biološku raznolikost među algalnim simbiontima. PAGE elektroforezom izolirano je ukupno pet izoenzima katalaze i pet izoenzima peroksidaze u endosimbiotskim algama. Izoenzim K1 pojavio se jedino kod endosimbiotske alge M9 iz Izraela, a izoenzim K3 jedino kod T soja. Izoenzim P1 pojavio se jedino kod endosimbionta HV, izoenzim P5 kod HV i M9. Izoenzimi P3 i P4 pojavili su se jedino kod algi BV i T iz Hrvatske. Ključne riječi: endosimbiotske alge, zelena hidra, simbioza, morfometrija, izoenzimska analiza HYDRA – ALGAE SYMBIOSIS: CHARACTERIZATION OF ENDOSYMBIOTIC GREEN ALGAE ISOLATED FROM DIFFERENT GREEN HYDRA STRAINS USING ELECTRON MICROSCOPY, MORPHOMETRIC AND ENZYME ANALYSIS N. Kević1, S. Radić Brkanac2, P. Peharec Štefanić3, F. Faraguna4, M. Kalafatić1, G. Kovačević1, D. Franjević1 1 Division of Zoology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia ([email protected]) 2 Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia 3 Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia 4 Faculty of Chemical Engineering and Technology, University of Zagreb, Zagreb, Croatia Process of endosymbiosis is one of promoters of speciation. The green hydra (Hydra viridissima Pallas, 1766) is a typical example of endosymbiosis. Its gastrodermal myoepithelial cells harbor the individuals of unicellular photoautotrophic algae. We have established prosperous laboratory cultures of endosymbiotic green algae isolated from green hydra strains. Hydra strains were from 6 different geographical localities: 4 from Croatia, 1 from Israel and 1 from Germany. Endosymbiotic green algae were successfully isolated using microbiological methods. Isolated endosymbiotic algae were characterized employing cytological morphometric parameters, enzyme activity and isoenzyme pattern analysis (catalase, peroxidase and th
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esterases). Cells of endosymbiotic algae were characterized by morphometric measurements: diameter, perimeter and area. Algal HV and M9 strains were separated from strains T and BV. The results obtained by isoenzyme pattern analysis suggest a difference between Croatian and European algal endosymbionts, indicating biological diversity among algal symbionts isolated from green hydras. Five isoenzymes of catalase and five isoenzymes of peroxidase were resolved by PAGE electrophoresis. Isoenzyme K1 appeared only in endosymbiotic algae M9 from Israel and isoenzyme K3 only in T strain. Isoenzyme P1 was observed only in endosymbiont HV while isoenzyme P5 in both endosymbionts HV and M9. Isoenzymes P3 and P4 were visible only in algal strains BV and T from Croatia. Key words: endosymbiotic algae, green hydra, symbiosis, morphometry, isoenzyme analysis P‐35 ErIK: SOFTVERSKA PLATFORMA ZA IDENTIFIKACIJU GUJAVICA HRVATSKE D. K. Hackenberger, B. K. Hackenberger, D. K. Hackenberger Odjel za biologiju, Sveučilište J. J. Strossmayera u Osijeku, Osijek, Hrvatska ([email protected]) Fauna beskralješnjaka u Hrvatskoj nedovoljno je istražena. Čest razlog neistraženosti je 'neaktraktivnost' svojti, ali i nedovoljna dostupnost ključeva za određivanje. Osobito su neistraženi beskralješnjaci tla, kako u Hrvatskoj tako i u Europi što se u posljednje vrijeme pokušava promijeniti s obzirom na značaj edafobionata za funkcionalnost i zdravlje ekoloških sustava. Jedna skupina edafobionata su gujavice (Lumbricidae), kojih je za faunu Hrvatske do sada zabilježeno 68 vrsta raspoređenih u 17 rodova. Osobitu faunističku značajnost čini deset endemskih vrsta. Kako bi se olakšalo njihovo određivanje razvili smo Ključ za određivanje vrsta gujavica Hrvatske ‐ ErIK u obliku računalnog programa. Ključ je dizajniran tako da se pomoću različitih alata i slika olakša određivanje do razine vrste. Glavni prozor programa sadrži opisne tekstove (vanjska i unutrašnja građa, distribucija), kartu pronalaska vrste, crtež vrste i popis svih vrsta u Hrvatskoj. Određivanje se može provesti na dva načina ‐ pomoću tabličnog i pomoću dihotomskog ključa. Tablični ključ omogućuje izravno upisivanje morfoloških i anatomskih parametara promatrane jedinke. Moguće je upisati ukupno 18 parametara, a nije potrebno upisati sve kako bi ključ radio. Dihotomski ključ temelji se na klasičnom ključu određivanja vrsta po principu dva izbora. Unutar programa se nalazi i priručnik za upotrebu s opisom svih morfoloških i anatomskih obilježja. Program je besplatan i dostupan za skidanje sa mrežne stranice. Ključne riječi: Lumbricidae, tablični ključ, dihotomski ključ ErIK: A SOFTWARE PLATFORM FOR IDENTIFICATION OF EARTHWORMS OF CROATIA D. K. Hackenberger, B. K. Hackenberger, D. K. Hackenberger Department of Biology, J. J. Strossmayer University in Osijek, Osijek, Croatia ([email protected]) The fauna of invertebrates in Croatia is insufficiently researched. A frequent reason lies in 'unattractiveness' of taxa and insufficient availability of determination keys. Particularly unexplored are soil invertebrates both in Croatia and Europe which has recently being changed due to the importance of edaphobionts for functionality and health of ecosystems. One group of edaphobionts are earthworms (Lumbricidae). So far 68 species distributed in 17 genera have th
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been recorded for Croatia, of which 10 endemic species have a particular faunistic significance. In order to facilitate their determination we have developed Earthworm Identification Key ‐ ErIK as a computer program. The key is designed so that usage of different tools and images facilitates determination to species level. The main window contains descriptions (anatomy and morphology, distribution), map with species records, drawing of the species and the list of all species in Croatia. Determination can be carried out in two ways, using tabular and dichotomous key. The tabular key enables entering morphological and anatomical parameters of observed individuals. A total of 18 parameters can be inserted, but not all for key to function. The dichotomous key is based on a classic determination key with two choices. There is a manual with description of all morphological and anatomical characteristics within the software. The software is free and available for download from a website. Key words: Lumbricidae, tabular key, dichotomous key P‐36 FLORA OTOČIĆA ŠOLTANSKOG ARHIPELAGA M. Ruščić1, M. Šeput2 1 Prirodoslovno‐matematički fakultet Split, Sveučilište u Splitu, Split ([email protected]) 2 Osnovna škola Cavtat, Cavtat Istraživana je flora 7 otočića: Stipanska, Balkun, Rudula, Polebrnjak, Grmelj, Saskinja i hrid Kamik, smještenih jugozapadno od obala otoka Šolte. Utvrđena je velika raznolikost vaskularne flore, najveći broj vrsta je zastupljen na otočiću Stipanska i iznosi 141 vrstu. Najzastupljenije porodice u ukupno istraživanoj flori su Fabaceae (10,55%), Poaceae (8,69%) i Asteraceae (5,59%). Najveći broj vrsta i podvrsta flore otočića pripada mediteranskom flornom elementu (63,96%) i to opće mediteranskim biljkama (64%). U okviru mediteranskog elementa veliko značenje imaju ilirsko‐mediteranske biljke (9,7%) te u sklopu njih ilirsko‐ jadranske endemične biljke (9,87%). Analizom životnih oblika flore otočića utvrđena je najveća zastupljenost terofita (30,43%), potom fanerofita s (24,85%) i hemikriptofita (18,63 %). U flori otočića utvrđeno je 5,6 % endemičnih biljaka. Istražene su i ugrožene svojte (2%), zaštićene (12%) i strogo zaštićene svojte (6,8%). Ključne riječi: flora, Stipanska, Balkun, Rudula, Polebrnjak, Grmelj, Saskinja, Kamik, otočići, endemi
THE FLORA OF ŠOLTA ARCHIPELAGO ISLETS
M. Ruščić1, M. Šeput2 1 Faculty of Sciences Split, University of Split, Split ([email protected]) 2 Elementary School Cavtat, Cavtat
We investigated the flora of 7 islands: Stipanska Balkun, Polebrnjak, Grmelj, Saskinja and rock Kamik, located south‐west coast of the island of Solta. There was a great diversity of vascular flora, the largest number of species is represented on the island Stipanska and is 141 species. The most abundant families in the studied flora of the Fabaceae (10.55%), Poaceae (8.69%), and the Asteraceae (5.59%). The highest number of species and subspecies of flora islands belong to the Mediterranean floral element (63.96%) and general Mediterranean plants (64%). Within the Mediterranean element of great significance have Illyrian and Mediterranean plants (9.7%) and within them Illyrian‐Adriatic endemic plants (9.87%). The analysis of life forms flora islands established a major presence Therophyta th
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(30,43%), then Phanerophytes with (24.85%) and Hemikryptophytes (18.63%). The flora of the islands was found 5.6% of endemic plants. Have been investigated and threatened species (2%), protected (12%) and strictly protected species (6.8%).
Key words: flora, Stipanska, Balkun, Rudula, Polebrnjak, Grmelj, Saskinja, Kamik, endems
P‐37 ANALIZA DIJELA HERBARIJA CARLA STUDNICZKE (ORD. CAPRIFOLIACEEN, LONICEREEN I STELLATEEN) N. Ževrnja¹, B. Mitić2, D. Vladović¹ ¹Prirodoslovni muzej i zoo, Split, Hrvatska ([email protected]) 2 Botanički zavod, PMF Sveučilišta u Zagrebu, Zagreb, Hrvatska Analizirani su redovi originalno označeni kao 'Lonicereen', 'Caprifoliaceen' i 'Stellateen' (157 herbarijskih listova). Najveći dio biljaka sakupljen je u Europi (146 listova), a najviše s područja današnje Austrije (30 listova). Prema pripadnosti pojedinim herbarijima ističu se biljke iz zbirke 'Flora Dalmatiens'. U odnosu na dosada obrađeni dio herbarija navode se nove zbirke: Flora d. from Brandeburg, Herb F. Tremols i Herbier de la Société d'Histoire Naturelle de Colmar. Najviše biljnog materijala sakupio je sam Studniczka (92 herbarijska lista). Najstariji herbarijski list je iz 1856. god., a najmlađi iz 1904. god. Studniczka unutar 157 herbarijskih listova navodi 15 rodova s 83 vrste u okviru kojih je zabilježeno 19 varijeteta. Ključne riječi: Prirodoslovni muzej Split, herbarij, Studniczka THE ANALYSIS OF PART OF CARL STUDNICZKA'S HERBARIUM (ORD. LONICEREEN, CAPRIFOLIACEEN AND STELLATEEN) N. Ževrnja¹, B. Mitić2, D. Vladović¹ ¹The Natural Science Museum and Zoo Garden, Split, Hrvatska ([email protected]) 2 Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia Orders originally assigned as 'Lonicereen', 'Caprifoliaceen' and 'Stellateen' (157 herbarium sheets) were analysed. The majority of herbarim material was collected in Europe (146 sheets) in the area of Austria (30). Most herbarium sheets belong to Flora Dalmatiens collection. In reference to the part of Studniczka's herbarium which has already been analysed, there are some new collections: Flora d. from Brandeburg, Herb F. Tremols and Herbier de la Société d'Histoire Naturelle de Colmar. Most herbarium sheets were collected by Studniczka himself (92). The oldest herbarium sheet dates from the year 1856 and the newest one is from 1904. According to Studniczka, within 157 sheets 15 genera with 83 and 19 varietets were recorded. Key words: Studniczka's herbarium, Natural History Museum Split th
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1. BALKANSKI HERPETOLOŠKI SIMPOZIJ U SUORGANIZACIJI S HRVATSKIM HERPETOLOŠKIM DRUŠTVOM 1st BALKAN HERPETOLOGICAL SYMPOSIUM IN CO‐ORGANIZATION WITH CROATIAN HERPETOLOGICAL SOCIETY P‐38 POPULACIJSKA EKOLOGIJA I SPOLNI DIMORFIZAM LIVADNE GUŠTERICE Lacerta agilis, LINNAEUS 1758 S DINARE U HRVATSKOJ S. Baškiera1, M. Vucić2, D. Jelić1 1 Hrvatski institut za biološku raznolikost, Hrvatsko herpetološko društvo HYLA, Zagreb, Hrvatska ([email protected]) 2 Sveučilište u Zagrebu, Prirodoslovno‐matematički fakultet, Biološki odsjek, Zoologijski zavod, Zagreb, Hrvatska Livadna gušterica, Lacerta agilis Linnaeus, 1758 je mali gušer koji naseljava Europu i istočni dio Azije, uključujući i veći dio Hrvatske. Početkom kolovoza 2012. godine, proveli smo istraživanje ove vrste na planini Dinara u Hrvatskoj. Tijekom tog istraživanja smo uhvatili i izmjerili 100 jedinki livadne gušterice koje smo kasnije podijelili u dvije grupe na temelju spola. Jedna grupa je sadržavala 56 odraslih ženki dok je druga sadržavala 40 odraslih mužjaka. Ulovljene su i četiri juvenilne jedinke koje su izostavljene iz statističke analize. Za svaku je jedinku zabilježen set ekoloških i stanišnih parametara po ulovu. A prije puštanja je za svaku jedinku izmjerena masa (m) i sljedeće morfometrijske karakteristike: ukupna dužina tijela ženke mjerena od vrha njuške do kraja repa (Ltot), dužina tijela ženke od vrha njuške do kloake (SVL), d užina repa (Lt), dužina od vrha njuške do kraja slušnog otvora (Lhel), dužina od vrha njuške do prednjeg uda (Lsfl), duljina od prednjeg do stražnjeg uda (Lfhl), visina glave (HH) i širina glave (HW). Sve su jedinke ulovljene na visokoplaninskom travnjaku između 1000 i 1400 mnm sa središnjom temperaturom staništa od 24,9 °C. Srednja tjelesna temperatura gušterica bila je 27,7 °C bez statistički značajne razlike između dvije grupe. Na temelju analize morfometrijskih podataka, zabilježen je spolni dimorfizam u tri kategorije: SVL, Lt i tjelesna masa. Oko 60% ulovljenih jedinki je imalo regeneriran rep što upućuje na visoki predatorski pritisak.
Ključne riječi: Lacerta agilis, dimorfizam, morfometrija
POPULATION ECOLOGY AND SEXUAL DIMORPHISM IN POPULATION OF THE SAND LIZARD, Lacerta agilis, Linnaeus 1758 ON DINARA MOUNTAIN, CROATIA
S. Baškiera1, M. Vucić2, D. Jelić1 1 Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia ([email protected]) 2 Faculty of Science, Department of Biology, Division of Zoology, Zagreb, Croatia
The sand lizard, Lacerta agilis Linnaeus, 1758 is a small lacertid lizard distributed across Europe and eastern Asia. Sand lizard also inhabits most of Croatia and is proclaimed strictly protected by Croatian Nature Protection Act. In August 2012, we conducted a research on Dinara Mountain in Croatia. Dinara is the highest Croatian mountain stretched from Dalmatia over to Bosnia and Herzegovina. During this research we collected and measured one hundred specimens. Later on, we put them into two groups according to sex. One group consisted of 56 females and other of 40 males. Four juveniles were captured during this research but were excluded from following statistical analysis. For each individual a set of ecological and habitat parameters was taken within th
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minutes from capture. Before release, body mass (m) and following morphometric characters were taken: total body length (Ltot), snout‐to‐vent length (SVL), tail length (Lt), snout to ear ‐ end length (Lhel), snout to forelimb length (Lsfl), forelimb to hind limb length (Lfhl), head height (HH) and head width (HW). All individuals were collected on high mountain grasslands between 1000 and 1400 m a.s.l. with mean habitat temperature of 24.9 °C. Mean body temperature was 27.7 °C with no significant difference between sexes. Morphological data indicate that there is statistically significant sexual dimorphism in SVL, Lt and body mass. Almost 60% of captured individuals had regenerated tails, indicating high predation pressure. Key words: Lacerta agilis, dimorphism, morphometry
P‐39 DOPRINOS POZNAVANJU HERPETOFAUNE DINARE U HRVATSKOJ I BLIŽE OKOLICE
S. Baškiera1, M. Vucić2, B. Lauš1, I. Vilaj1 1 Hrvatski institut za biološku raznolikost, Hrvatsko herpetološko društvo HYLA, Zagreb, Hrvatska ([email protected]) 2 Sveučilište u Zagrebu, Prirodoslovno‐matematički fakultet, Biološki odsjek, Zoologijski zavod, Zagreb, Hrvatska Dinara je planina smještena u Dinarskom gorju na granici Hrvatske i Bosne i Hercegovine. Izgrađena je od karbonatnih stijena i duga 84 kilometra. Vrhom Dinara ili Sinjal (1831 mnm) čini najvišu planinu u Hrvatskoj. Ovo planinsko, nepristupačno područje je nedovoljno istraženo, dijelom i zbog miniranog terena. Tijekom svibnja i kolovoza 2012. godine, Udruga studenata 'BIUS' provodila je istraživačko‐edukacijski projekt 'Dinara 2012'. Cilj našeg dijela istraživanja je bio kvalitativno ispitivanje sadržaja herpetofaune Dinare. Literaturni podaci navode 20 vrsta vodozemaca i gmazova koji žive na ovim područjima. Našim istraživanjem je zabilježeno sveukupno 16 vrsta: 13 vrsta gmazova i 3 vrste vodozemaca, uključujući i vrste pronađene na putu od Kina do Dinare. Najčešće zabilježena vrsta je bila livadna gušterica, Lacerta agilis, a najznačajniji je bio pronalazak planinskog žutokruga, Vipera ursinii, koji je regionalni endem ovog područja, vrsta koja je uvrštena u Crvenu knjigu vodozemaca i gmazova Hrvatske te strogo zaštićena Zakonom o zaštiti prirode. Ključne riječi: Dinara, vodozemci, gmazovi CONTRIBUTION TO THE KNOWLEDGE OF HERPETOFAUNA OF CROATIAN PART OF DINARA MOUNTAIN AND ITS VICINITY S. Baškiera1, M. Vucić2, B. Lauš1, I. Vilaj1 1 Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia ([email protected]) 2 Faculty of Science, Department of Biology, Division of Zoology, Zagreb, Croatia Dinara is a mountain located in the large mountain chain Dinarides on the border between Croatia and Bosnia and Herzegovina. It is made of carbonate rocks and is 84 kilometers long. Its peak Sinjal, also called Dinara (1831 m a.s.l.), is the highest mountain peak in Croatia. Due to its limited accessibility and mined areas, knowledge of Dinara's flora and fauna is incomprehensive. Biology Students Association – BIUS carried out two field trips to mountain Dinara, one in May and one in August 2012. Main goal of our research was to make a quantitative list of herpetofauna of researched parts of Mt. Dinara. Literature data states th
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that 20 species of amphibians and reptiles inhabit these areas. Our inventory list counts 13 reptile and 3 amphibian species (16 in total), including species found on hiking trail to mountain Dinara from town Knin. The most frequently recorded species was the sand lizard, Lacerta agilis. The most important finding was Orsini's viper, Vipera ursinii, which is a regional endemic species of the area, a species that is listed in the Croatian Red Book of Amphibians and Reptiles and strictly protected by the Croatian Nature Protection Act. Key words: Dinara, amphibians, reptiles P‐40 IS POPULATION OF MOOR FROG (Rana arvalis) UNDER SEVERE DECLINE IN MURA RIVER BASIN IN SLOVENIA? M. Cipot, A. Lešnik, K. Poboljšaj 1 Center for Cartography of Fauna and Flora, Miklavž na Dravskem Polju, Slovenija ([email protected]) Distribution of Rana arvalis in Slovenia is well known. Since it is a charismatic species in Slovenia, there are many known observations by enthusiast and only in few occasions data are results of systematic research. As a result population conservation status and population size and trends are in majority unknown. The only systematic survey targeted for Rana arvalis was made in 2011 on Ljubljansko Barje and in spring 2013 and 2014 on a large part of Mura river Basin (presented here). We performed a complete screening of Natura 2000 site Mura from Gornja Radgona to Vučja vas. An insight of population size and density in this area was obtained by egg mass counting and counting of blue adult males. Data are also available for some other major spawning sites of the species in Mura basin (from years 2013‐ 2014, 2011 and 2008 respectively). Numbers gathered during our study were surprisingly low. There are only few known localities with high number of breeding animals (more then 100 egg mass or adult males counted) in the Mura river basin: oxbows Zaton (Petanjci), Bunčani, Krapje (Veržej), Bobri (Gornja Bistrica), Muriša (Lendava) and forest Polanski log (Velika Polana) and forest Črni log (Lendava). We noticed a severe decline of populations in the area in the last decade. The results of egg mass counts in Polanski log between years 2011‐2014 showed that in 2014 only one tenth of numbers of 2011 were counted. A similar trend of decline is observed from 2008 to 2013 from Zaton oxbow. Exact causes for the decline are not known, but several natural and anthropogenic factors such as pollution, habitat disappearance and degradation are in place. Potential causes for the decline are the drainage of the oxbows due to lowering of the ground water level, afforestation of oxbow banks and shading of breeding sites and large forest clear cuts. Moor frogs namely prefer to deposit eggs in shallow water on the banks of oxbows, with higher water temperatures in springtime whereas later in summer these areas often dry out. We cannot say weather 90% decline in the numbers of moor frogs gathered in last few years are a sign of population decline heading toward local extinctions, but the results emphasize the need for further population monitoring and better identification of threats. Key words: Moor frog, Rana arvalis, Slovenia, river Mura, population decline th
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P‐41 HERPETOFAUNA OF POSAVINA REGION IN BOSNIA AND HERZEGOVINA AND CROATIA A. Ćurić1, A. Zimić2, D. Jelić3 1 Society for Research and Protection of Biodiversity, Banja Luka, Bosnia and Herzegovina ([email protected]) 2 Herpetological Association in Bosnia and Herzegovina ATRA, Sarajevo, Bosnia and Herzegovina 3 Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia Posavina region received its name from Sava River, which dominates its landscape, and it incorporates a large part of the surrounding hilly areas and lowlands of large tributary rivers. Border area is still mine polluted, especially on B&H side, and there is a serious lack of data for many species groups, including herpetofauna. During year 2014 and 2015 we collected significant new data for B&H and Croatia, which we combined with known literature data, data from museum collections and local expert personal data in order to present updated list of Posavina herpetofauna. Field work was done during day and night and all species were recorded and usually photographed. We were strictly focused on lowland areas (up to 300 m a.s.l.) with typical riparian habitats. In Posavina region we found total of 17 amphibian and 12 reptile species. Specifically for B&H we recorded all together 14 amphibian species (we highlight Pelobates fuscus and Pelophylax lessonae as species confirmed for the first time) and 11 reptile species (we highlight Lacerta agilis argus and Vipera berus bosniensis also first time confirmed). One individual of Pelodiscus sinensis, allochthonous species, has been caught on Bardača in 2010 and moved into captivity. Croatia had much more reliable literature data and combined with new personal data we arrive to the number of 17 amphibian and 11 reptile species. The most interesting data for Croatia are lowland populations of Zootoca vivipara and Ichtyosaura alpestris. Key words: amphibia, reptilia, lowland, distribution, Pannonian Basin P‐42 EFFECT OF ENVIRONMENTAL TEMPERATURE ON BODY CONDITION AND JUMPING PERFORMANCE OF TWO GEOGRAPHICALLY DISTANT Rana temporaria POPULATIONS S. Drakulić1, H. Feldhaar2, D. Lisičić3, M. Mioč3, I. Cizelj4, M. Seiler2, T. Spatz5, M.‐O. Rödel1 1 Museum für Naturkunde, Leibniz Institute for Evolution and Biodiversity Science, Berlin, Germany ([email protected]‐berlin.de) 2 Department of Animal Ecology I, University of Bayreuth, Bayreuth, Germany 3 Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia 4 Zoological garden of Zagreb, Zagreb, Croatia 5 Department of Animal Ecology and Tropical Biology, University of Würzburg, Würzburg, Germany Environmental temperatures affect all physiological processes and performance of ectotherms. Performance directly influences fitness by determining the probability of survival. Therefore, physiological adjustments to local thermal environment are extremely important for achieving optimal performance levels. Rana temporaria is a widely distributed European frog, present in a variety of habitats. This makes it an exceptional model for studying population‐specific physiological adjustments to the local environmental conditions. We hypothesized that R. temporaria populations from colder (Central Europe) and warmer (Southeast Europe) th
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environments show population‐specific variation in developmental time, morphometric traits and jumping performance, as a consequence of differences in local thermal conditions. We raised tadpoles from two populations under the same set of developmental conditions – in two constant temperature treatments, and in a semi‐natural setup, in garden. After metamorphosis, we compared the influence of environmental temperatures on duration of developmental period, morphometric traits and jumping performance among froglets from different treatments, within and across populations. We detected significant effect of developmental temperatures on all measured traits. Moreover, we found significant differences in those traits between different populations, indicating that reaction mechanisms to environmental temperatures are population‐specific. Key words: frog, environmental temperature, physiological adjustment, climate change P‐43 SPATIAL REPLACEMENT OF CLOSE RELATIVES, A PASSIONATE STORY OF A MOUNTAINOUS POPULATION OF Vipera ammodytes A. Dyugmedziev, N. Tzankov, G. Popgeorgiev Vertebrates Department, National Museum of Natural History – BAS, Sofia, Bulgaria ([email protected]) We studied the uppermost part of Struma valley in Western Bulgaria, where the nominate subspecies and V. ammodytes montandoni come into contact. Historically, till the mid‐nineties only the nominate subspecies has been found regularly there. In the mid‐nineties, however, multiple farms for venom went bankrupt and thus an unknown number of specimens were released outside of their native range; unconfirmed data suggests that hundreds of snakes were incidentally released close to the study area. When a single V. a. montandoni was found in 2008, an incidental introduction as well as a possible natural penetration in the study area was assumed. During the next years, intensive studies revealed virtually a complete exchange of the nominate subspecies by V. a. montandoni. In order to evaluate the degree of this process, spatial analyses with Maxent software of the existing locations were carried out together with six geographic and same number climatic variables. The analyses demonstrated a great degree of spatial niche overlap (73%) between the two taxa. The additional analysis of the locations raster values revealed statistically significant difference in two parameters, namely the potential evapo‐transpiration, where the nominate ssp. was with higher values, being more related to places with higher overall humidity, and the altitude, where V. a. montandoni showed a more narrow altitudinal range. Likely, recent global and local climate changes contributed to the observed process that could also be a natural distribution shift. Key words: invasion, ecological substitution, co‐ocurrence P‐44 FAUNISTIČKI NALAZI VODOZEMACA I GMAZOVA U OKOLICI NAŠICA D. Hlavati1, M. Zadravec2 1 Josipa Jurja Strossmayera 100, Našice, Hrvatska ([email protected]) 2 Hrvatsko herpetološko društvo – Hyla, Zagreb Hrvatska th
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U novije vrijeme nije bilo mnogo fanustičkih istraživanja herpetofaune na području Slavonije. U ovom radu prikazani su nalazi istraživanja vodozemaca i gmazova na osam lokaliteta u okolici Našica od 2010. do 2014. godine. Vodozemci su hvatani mrežama ili rukom, a gmazovi samo rukom. Jedinke se fotografiralo i pustilo neozljeđene nazad na mjesto ulova. Ukupno je zabilježeno devet vrsta vodozemaca i devet vrsta gmazova. Gotovo sve zabilježene vrste su očekivane, uz iznimku invazivne crvenouhe kornjače, Trachemys scripta elegans (Wied‐ Neuwied, 1839). Nađena je jedna odrasla jedinka u umjetnom jezeru u gradskom parku u Našicama, 2010. Vrlo vjerojatno je riječ o namjerno puštenom kućnom ljubimcu. Dvije zabilježene vrste, žuti mukač, Bombina variegata (Linaneus, 1758) i barska kornjača, Emys orbicularis (Linnaeus, 1758), koji su na Dodacima II i IV Direktive o staništima Europske komisije. Kroz ovo istraživanje prikupljeni su prvi konkretni podaci o prisutnosti vrsta vodozemaca i gmazova na tom području Hrvatske i može koristiti kao podloga za buduća istraživanja. Ključne riječi: herpetofauna, Natura 2000, Slavonija FAUNISTIC RECORDS OF AMPHIBIANS AND REPTILES IN THE NAŠICE AREA D. Hlavati1, M. Zadravec2 1 Josipa Jurja Strossmayera 100, Našice, Croatia ([email protected]) 2 Croatian Herpetological Society – Hyla, Zagreb, Croatia In recent times there has not been a lot of faunistic research of the herpetofauna in the area of Slavonija. Here we give the list of amphibians and reptiles found on eight localities around Našice, from 2010 till 2014. Amphibians were caught by nets or by hand, while reptiles were caught only by hand. Caught individuals were photographed and released unharmed immediately afterwards at the same place where they had been found. A total of nine amphibian and nine reptile species were found. Almost all of the species had been expected for the area, except the invasive red‐eared slider, Trachemys scripta elegans (Wied‐Neuwied, 1839). One adult individual was found in the artificial lake in the city park in Našice, in 2010. Most likely it is an intentionally introduced pet. Two of the species we found, the yellow‐bellied toad, Bombina variegata (Linaneus, 1758) and the European pond terrapin, Emys orbicularis (Linnaeus, 1758) are on Annexes II and IV of the European Commision's Habitats Directive. This research yields the first exact records on the occurence of amphibians and reptiles in this part of Croatia and can be used as a basis for future work. Key words: herpetofauna, Natura 2000, Slavonia P‐45 MOLEKULARNA FILOGENIJA IVANJSKOG ROVAŠA (Ablepharus kitaibelii) NA SREDIŠNJEM I SJEVERNOM DIJELU PODRUČJA RASPROSTRANJENOSTI D. Jelić1, M. Jelić2, M. Futo3, B. Halpern4, K. Ljubisavljević5,6, N. Tzankov7 1 Hrvatski institut za biološku raznolikost, Hrvatsko društvo za biološka istraživanja, Zagreb, Hrvatska ([email protected]) 2 Sveučilište u Zagrebu, Prirodoslovno‐matematički fakultet, Biološki odsjek, Zoologijski zavod, Zagreb, Hrvatska 3 University of Münster, Institute for Evolution and Biodiversity, Animal Evolutionary Ecology Group, Münster, Njemačka th
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4
MME BirdLife Hungary, Budimpešta, Mađarska Univerzitet u Beogradu, Institut za biološka istraživanja „Siniša Stanković“, Beograd, Srbija 6 Crnogorsko društvo ekologa, Podgorica, Crna Gora 7 Natural Museum of Natural History, Vertebrates Department, Sofija, Bugarska Ivanjski rovaš je termofilna vrsta guštera i spada među najmanje gmazove Europe. Rasprostranjen je od Slovačke, prema jugu Europe sve do Turske. U Hrvatskoj je nedavno potvrđena njegova prisutnost na obroncima Papuka i u gradu Iloku, što čini sjeverozapadnu granicu rasprostranjenosti. Do sada su korištenjem mitohondrijskih molekularnih biljega (16S rRNA i citokrom b) istraživani filogenetski odnosi populacija ivanjskog rovaša u južnom dijelu areala. Zaključeno je da ivanjski rovaš nije monofiletska skupina, već kompleks vrsta. Cilj ovog istraživanja je da se korištenjem mitohondrijskih biljega, istraže populacije na ostatku područja rasprostranjenosti u Europi, kako bi se upotpunili zaključci o filogeniji. Zabilježeni haplotipovi iz rumunjskih populacija, odvojili su se kao nova zasebna linija na filogenetskom stablu. Međutim, genetska varijabilnost populacija s područja Hrvatske, Srbije, Bugarske i Makedonije je izrazito niska. Na cijelom tom području, zabilježena je prisutnost samo jednog haplotipa na 16S rRNA i citokrom b biljegu. Haplotipovi su istovjetni onima iz kontinentalne Grčke. Taj rezultat ukazuje na naglo širenje područja rasprostranjenosti, najvjerojatnije iz izvorišne populacije s područja Grčke. Naša hipoteza je da se naglo širenje zbilo u najtoplijem razdoblju zadnjeg interglacijala koje odgovara razdoblju atlantika (prije 8000 do 5000 godina). Ključne riječi: termofilna vrsta guštera, Balkanski poluotok, pleistocenska pribježišta, brzo širenje areala, holocen MOLECULAR PHYLOGENY OF THE SNAKE‐EYED SKINK (Ablepharus kitaibelii) ON THE CENTRAL AND NORTH PART OF ITS DISTRIBUTION RANGE D. Jelić1, M. Jelić2, M. Futo3, B. Halpern4, K. Ljubisavljević5,6, N. Tzankov7 1 Croatian Institute for Biodiversity, Croatian Biological Research Society, Zagreb, Croatia ([email protected]) 2 University of Zagreb, Faculty of Science, Division of Biology, Department of Zoology, Zagreb, Croatia 3 University of Münster, Institute for Evolution and Biodiversity, Animal Evolutionary Ecology Group, Münster, Germany 4 MME BirdLife Hungary, Budapest, Hungary 5 University of Belgrade, Institute for Biological Research „Siniša Stanković“, Belgrade, Serbia 6 Montenegrin Ecologists Society, Podgorica, Montenegro 7 Natural Museum of Natural History, Vertebrates Department, Sofia, Bulgaria The Snake‐eyed Skink is a small thermophilous lizard. It is distributed from Slovakia, towards south of Europe all the way to Turkey. In Croatia, this species has been recently recorded in the Papuk Mountain and in the city of Ilok, forming its western distribution border. Up to now, mitochondrial molecular markers (16S rRNA and cytochrome b) have been used to assess the phylogeny of the Snake‐eyed Skink populations in the southern part of its distribution range. It was concluded that the species does not form a monophyletic group; rather it is a complex of several species. The aim of our research was to examine populations from the remaining parts of distribution range in Europe, using mitochondrial markers. Obtained haplotypes from a Romanian population, form a new distinct lineage on the phylogenetic tree. However, extremely low genetic variability was observed in Croatia, Serbia, Bulgaria and Macedonia, where only a single haplotype was detected on each particular mitochondrial marker. The 5
th
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same haplotypes were reported for the Central Greece. This result indicates a rapid range expansion, from ancestral population most probably situated in Greece. Being a thermophilous species, we suppose that the Snake‐eyed Skink’s expansion happened in the warmest periods during the Holocene, corresponding to the Atlantic period (8000‐5000 years BP).
Key words: thermophilous lizard species, Balkan Peninsula, Pleistocene refugium, rapid range expansion, Holocene
P‐46 NOVI DOKAZI O POSTOJANJU TROGLOKSENIH PRILAGODBI HERPETOFAUNE DINARSKOG KRŠA
K. Koller1,2, P. Konrad Kovač1,2 1 Hrvatsko herpetološko društvo HYLA, Zagreb, Hrvatska ([email protected]) 2 Hrvatski institut za biološku raznolikost, Zagreb, Hrvatska Ulazak vodozemaca u podzemlje Dinarskog krša do sada nije detaljnije promatran. U literaturnim podacima su do sada zabilježeni slučajni ulasci vrstau podzemlje, ali njima se nije pridavala posebna pažnja.U ovom radu pružali smo više dokaza o ovom fenomenu i dali smo osnovan pregled literaturnih podataka. 14. i 15. travnja 2015. godine organizirano je speleoronilačko istraživanje Pazinske jame, s glavnim ciljem pronalaska našeg krškog endema čovječje ribice (Proteus anguinus). Pazinska jama je smještena u Pazinu (Istra, Hrvatska) (N 5416357; E 5011182, 185 m n.v.). Tok rijeke Pazinčice je dug 16,5 km te ponire u Pazinsku jamu. Nakon ulaza slijedi sto metara dug kanal koji se proširuje u dvoranu (80 * 20 m) s velikim podzemnim jezerom (Martelovo jezero). Nakon detaljnog pregleda jezera, sifona i kanala, čovječja ribica nije pronađena, ali su pronađene 20 jedinki lombardijske smeđe žabe (Rana latastei), dvije jedinke smeđe krastače (Bufo bufo) te jedna jedinka riječne kornjače (Emys orbicularis). Sve tri vrste su zamijećene u najudaljenijem suhom dijelu jame. Vrste su uhvaćene, fotografirane i potom puštene. Ovo je prvi nalaz lombardijske smeđe žabe i riječne kornjače u špilji u Hrvatskoj, i to na mjestu gdje je prisutna potpuna tama. Duž plaže Martelovog jezera zabilježene su i nakupine jaja smeđe krastače. Nije primjećeno da su vrste pothranjene. Uočeno je da je vanjska obojanost smeđe krastače bila nešto svijetlija od uobičajene. Budući da je u Pazinskoj jami zabilježen ovako veliki broj vodozemaca, te da postoje i druge indikacije o prisutnosti vodozemaca u podzemlju, naša hipoteza je da bi ove vrste mogle biti ne samo stigoksene, već možda i stigofilne vrste.
Ključne riječi: podzemlje, vodozemci, Rana latastei, Pazinska jama, dinarski krš
NEW EVIDENCE TO EXISTENCE OF TROGLOXENE ADAPTATIONS IN HERPETOFAUNA OF DINARIC KARST K. Koller1,2, P. Konrad Kovač1,2 1 Croatian Herpetological Society HYLA, Zagreb, Croatia ([email protected]) 2 Croatian Institute for Biodiversity, Zagreb, Croatia
Entering of amphibians into the underground of Dinaric Karst has received very little attention in published literature. There is only literature data about the accidental access of amphibians into the underground, but without any special overview. Here we provide more evidence on this phenomenon and give basic overview of previous records. During the 14th and 15th of April 2015 we made speleological exploration of Pazinska cave with the main purpose of prospecting for the Proteus anguinus. Enterance of Pazinska cave is located in Pazin (Istria, Croatia) at about 185 m a.s.l.. Watercourse of river Pazinčica is 16.5 km long and plunges into Pazinska cave. Behind the th
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entrance there is a hundred meter long channel which expands into the hall (80 * 20 m) with a large cave lake (Martel Lake). After detailed research of the lake, siphons and channels, the Proteus has not been found, but we made observation of 20 individuals of Italian agile frog (Rana latastei), two individuals of Common toad (Bufo bufo) and one individual of European pond turtle (Emys orbicularis). All of these three species were observed in the deepest dry part of the cave. The specimens were caught, photographed and released.This is the first finding of Italian agile frog and pond turtle in Croatia inside the cave, where complete darkness is presented. Along the sandy edge of the underground lake a stripes of Bufo bufo eggs were also noticed. There was no indication of starvation in species. It was observed that the external coloration of the Bufo bufo speciments was somewhat brighter than usual. Because of such a big number of amphibians in Pazinska cave and because of the other indications about the presence of this species in the caves, our hypothesis is that these species may be not only stygoxene, but maybe also stygophile.
Key words: underground, amphibians, Rana latastei, Pazinska cave, Dinaric Karst
P‐47 COASTAL POPULATION OF DICE SNAKES (Natrix tessellata) HELPS CLARIFY THE EVOLUTION OF EURYHALINE PHYSIOLOGY IN MARINE TETRAPODS Y. Kornilev1, F. Brischoux2 1 National Museum of Natural History, Bulgarian Academy of Sciences, Sofia, Bulgaria ([email protected]) 2 Centre d’Etudes Biologiques de Chizé, Villiers en Bois, France The widespread relationship between salt excreting structures (e.g. salt glands) and marine life strongly suggests that the ability to regulate salt balance has been crucial during the transition to marine life in tetrapods. However, lacking data from intermediate stages (species lacking salt glands but occasionally using saline environments) preclude to draw a comprehensive picture of the evolution of euryhaline physiology in these organisms. Populations of Dice snakes (Natrix tessellata) foraging in the marine environment along the coast of the Black sea provide a unique opportunity to study physiological adaptations to marine life in a marine snake prototype lacking salt glands. We show that coastal free‐ranging Dice snakes display highly variable natremia (plasma sodium concentration) ranging from normonatremia (~145 mmol L‐1) up to strong hypernatremia (i.e. >195 mmol L‐1). Our laboratory experiments indicate that this species can sustain life in full‐strength seawater (~35 g of salt per L) up to one week with no visible long‐term damage, but that such treatment entailed a marked hypernatremia that can be regulated only trough access to freshwater. Taken together these results suggest that hypernatremic Dice snakes require regular access to freshwater in order to periodically restore osmotic balance. Our results further suggest that the development of a strong physiological tolerance toward deviations of the osmotic balance (e.g., increased plasma sodium) has been a critical innovation in the evolution of aeuryhaline physiology and may well have preceded the evolution of salt glands. Key words: salinity, natremia, marine life P‐48 A PRELIMINARY STUDY ON THE BIOACTIVITY OF THE MICROBIOTA IN THE POST‐CAECAL GI TRACT OF THREE Testudo SPECIES (TESTUDINIDAE, TESTUDINES) WITH EMPHASIS ON LACTIC ACID BACTERIA Y. Kornilev1, S. Etem2, N. Natchev2,3, I. Ivanchev4, N. Tzankov1, T. Ignatova‐Ivanova2 th
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1
Section Vertebrates, National Museum of Natural History, Bulgarian Academy of Sciences, Sofia, Bulgaria ([email protected]) 2 Faculty of Natural Science, Shumen University, Shumen, Bulgaria 3 Department of Integrative Zoology, Vienna University, Vienna, Austria 4 Gea Chelonia Foundation, Sofia, Bulgaria We investigated the microbiota inhabiting the colon of Testudo graeca, T. marginata and T. hermanni. We sampled fragments of fresh feces obtained from specimens kept in a rescue centre. Fecal samples were stored in sterile tubes, chilled to 6 °C and transported to the laboratory in cooling bags. Samples were incubated for 24 hours at 37 °C. The delivered inoculum (100 µl) was filtered over a sterile media MRS agar with staining agent X‐gal dissolved in 20 ml DMSO (dimethyl sulfoxide). Applying this specific staining, we identified Lactobacillus species. Out of three samples we isolated pure cultures that were then stained by the Gram method. As expected, the cultures were all Gram positive. We will perform DNA and biochemical analysis to identify the exact bacterial species. In addition, we conducted investigation on the antimicrobial and antifungal activities of the isolated bacterial strains (colonies) on eleven pathogen species. We compared the bioactivity of the colonies to those of two broad‐spectrum antibiotics ‐ Sefpotect and Chloronitromycin (250 mg/ml). Our results indicate that the investigated tortoise possess microflora which protects them from a variety of gastro enteric pathogens. Key words: tortoise, gastro enteric bacteria, pathogens P‐49 MORFOLOŠKE ZNAČAJKE ŠARE POLJARICE, Hierophis gemonensis (1768, Laurenti) P. Lisičar, B. Lauš, I. Vilaj, D. Jelić Hrvatski institut za biološku raznolikost, Hrvatsko herpetološko društvo HYLA, Zagreb, Hrvatska ([email protected]) Makar se šara poljarica smatra jednom od najčešćih vrsta hrvatskog primorja i otoka dosadašnji radovi ne obuhvaćaju njenu morfologiju i rasprostranjenost. Kako bi odredili točnu distribuciju vrste Hierophis gemonensis u Hrvatskoj koristili smo bazu podataka HHD Hyla sa potvrđenim GPS koordinatama. Cilj je također bio analizirati karakteristike i morfološke značajke vrste. Istraživanje je provedeno na uzorku od 50 jedinki sačuvanih u 70%‐tnom etanolu i 51 živih jedinki premjerenih tijekom terenskih istraživanja. Za svaku jedinku su zabilježena 3 meristička i 14 morfometrijskih obilježja. Ženke su uglavnom manje i lakše od mužjaka. Spolni dimorfizam je potvrđen u dužini repa te broju trbušnih i podrepnih ljuski. Rezultati ukazuju na značajne razlike u morfometrijskim značajkama između kopnenih i otočnih populacija vrste H. gemonensis. Ključne riječi: morfološke razlike, spolni dimorfizam, merističke značajke, Hrvatska DISTRIBUTION AND MORPHOLOGY OF BALKAN WHIP SNAKE, Hierophis gemonensis (1768, Laurenti) P. Lisičar, B. Lauš, I. Vilaj, D. Jelić Croatian Institute for Biodiversity, Croatian Herpetological Society HYLA, Zagreb, Croatia ([email protected]) th
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Although Balkan whip snake is considered one of the most common snakesofcostal Croatia and islands, there are no studies about morphological differences and distribution of Hierophis gemonensis to this day. To determine exact distribution of H. gemonensis we used HHD Hyla database of confirmed finding sites with GPS coordinates.Our goal was also to analyse characteristics of species morphology. The study was carried out on 50 specimens preserved in 70% ethanol and 51 living specimens measured during field studies. Specimens were examined for 3 meristic and 14 morphometric characters. Females showed tendency to be smaller and lighter than males. Sexual dimorphism is confirmed in tail length, and number of ventral and subcaudal scales. Results indicate that there is significant difference in morphometric characteristics between insular and land population of Balkan whip snake. Key words: morphological differences, sexual dimorphism, meristic, Croatia
P‐50 PRAĆENJE ZDRAVSTVENOG STANJA ČOVJEČJE RIBICE (Proteus anguinus) U ZATOČENIŠTVU M. Lukač1, E. Prukner‐Radovčić1, D. Horvatek1, I. Cizelj2, F. Martinković3, D. Jelić4, Ž. Gottstein1 1 Zavod za bolesti peradi s klinikom, Veterinarski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) 2 Ustanova Zoološki vrt grada Zagreba, Zagreb, Hrvatska 3 Zavod za parazitologiju i invazijske bolesti s klinikom, Veterinarski fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 4 Hrvatski institut za bioraznolikost, Zagreb, Hrvatska U sklopu projekta zaštite čovječje ribice u Hrvatskoj obavljaju se i in situ istraživanja životinja u zatočeništvu. Budući da nema podataka o fiziološkoj mikroflori, ni o mikroorganizmima koji mogu narušiti zdravstveno stanje čovječje ribice, svrha je ovog istraživanja steći što detaljniji uvid u zdravstveno stanje životinja, razlučiti fiziološku mikrofloru od oportunističkih mikroorganizama i pravih patogena, te u slučaju potrebe provesti ispravnu terapiju. Obavljaju se sljedeća testiranja: (1) Standardna mikrobiologija – obrisci usne šupljine, kloake i kože; (2) Parazitološka testiranja – izmet, strugotine crijeva i organi uginulih životinja – nativni preparati i otisci; (3) Testiranje na Batrachochytrium dendrobatidis (BD) i na ranavirus – obrisci kože metodom Real Time PCR; (4) Testiranje na Chlamydia sp. – obrisci usne šupljine i kloake metodom Real time PCR; (5) Razudba i patohistološka pretraga uginulih životinja – da se dobije što više podataka o anatomiji i funkciji pojedinih organa. Dosad je nađen veći broj mikroorganizama tla, poput Aeromonas hydrophila i Aspergillus flavus, koji u nepovoljnim uvjetima mogu uzrokovati bolesti vodozemaca, no nađene su i bakterije koje sudjeluju u sprječavanju zaraza vodozemaca BD‐om poput Janchtinobacterium lividum. Parazitološkom pretragom izmeta nađeni su samo pseudoparaziti iz vode i vlažnog tla. Sve dosad testirane životinje bile su negativne na BD, ranavirus i bakterije Chlamydia sp. Ključne riječi: čovječja ribica, zdravstveno stanje, mikroorganizmi, Real time PCR MONITORING OF HEALTH STATUS OF THE OLM (Proteus anguinus) IN CAPTIVITY M. Lukač1, E. Prukner‐Radovčić1, D. Horvatek1, I. Cizelj2, F. Martinković3, D. Jelić4, Ž. Gottstein1 th
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Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia ([email protected]) 2 Zagreb Zoo, Zagreb, Croatia 3 Department of Parasitology and Parasitic Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia 4 Croatian Institute for Biodiversity, Zagreb, Croatia
Within the conservation of Proteus anguinus (the olm) project in Croatia, besides the field observations, in situ investigation of the species is also carried out.Since there is a lack of information on physiological flora and microorganisms which can compromise the olm’s health, the aim of the veterinary part of the project is to learn as much as possible about the health status of these animals. In order to distinguish between physiological microflora, opportunistic and true pathogens, and to provide the right treatment, if necessary, the followings tests are performed:(1) Standard microbiology of oral cavity, cloacal and skin swabs; (2) Parasitological tests of feces, intestinal scrapings and organs of dead specimens – native preparations and prints; (3) Batrachochytrium dendrobatidis (BD) and Ranavirus tests ofskin swabs by the Real Time PCR method; (4) Chlamydia sp. test oforal cavity and cloacal swabs by the Real time PCR method; (5) Autopsy and pathohistologyof dead specimens, to get more information on various organ anatomy and function. So far, a larger number of soil microorganisms were identified, such as Aeromonas hydrophila and Aspergillus flavus, potential causes of disease in amphibians under suboptimal conditions. Bacteria species, such as Janchtinobacterium lividum, which could participate in prevention of BD infection in certain amphibian species were also identified. Parasitological tests of fecal samples revealed the presence of pseudoparasites from water. All the animals analyzed so far have been negative for BD, Ranavirus and Chlamydia sp.
Key words: Olm, health status, microorganisms, Real time PCR
P‐51 ISOENZYME PROFILES OF SUPEROXIDE DISMUTASE AND CATALASE IN LIVER AND MUSCLE SELECTED ANURAN SPECIES
N. Lukić, J. Friščić, G. Šukalo, R. Dekić, B. Kukavica‐Jovanović Faculty of Science and Mathematics, University Banja Luka, Banja Luka, Bosnia and Herzegovina ([email protected])
Reactive oxygen species (ROS) may cause cell damage and contribute to changes in cellular function. Superoxide dismutase (SOD) and catalase (CAT) represent key enzymes of cell antioxidative metabolism. This study focuses on first comparative analysis of CAT and SOD isoenzyme profiles found in liver and muscle of following anuran species: Rana graeca, Bombina variegata and Pelophylax kl. esculentus. Animals were collected at several localities in Bosnia and Herzegovina. SOD and CAT isoforms were identified using native polyacrylamide electrophoresis (PAGE). Native electrophoresis resolved the presence of one CAT isoform in liver of all three frog species, out of which the highest activity was observed in species Pelophylax kl.esculentus. In the muscle, CAT isoform was detected only in species Bombina variegata. Comparative analysis of liver and muscle SOD profiles showed gender and species correlated differences. Obtained results are discussed in terms of differences in the environments which these species inhabit in correlation with the antioxidative metabolism. th
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Key words: antioxidative metabolism, Rana graeca, Bombina variegata, Pelophylax kl. esculentus P‐52 ZMIJE U HERPETOLOŠKOJ ZBIRCI PRIRODOSLOVNOG MUZEJA U SPLITU, HRVATSKA S. Mekinić1,3, N. Ževrnja2, J. Boban2, G. Piasevoli1, D. Vladović2 1 Javna ustanova za upravljanje zaštićenim dijelovima prirode na području Splitsko‐dalmatinske županije 'More i krš', Split, Hrvatska ([email protected]) 2 Prirodoslovni muzej i zoološki vrt, Split, Hrvatska 3 Gata 73, Gata, Hrvatska Analizom herpetološke zbirke Prirodoslovnog muzeja u Splitu utvrđeno je da su zmije u herpetološkoj zbirci zastupljene kroz 183 inventarna broja sa 224 primjeraka. U navedenoj zbirci se nalaze primjerci 4 porodice, 5 potporodica, 11 rodova i 15 vrsta zmija. Najzastupljeniji su primjerci iz porodice Natricinae i Viperidae odnosno rodovi Natrix i Vipera. Najveći broj primjeraka prikupili su U. Girometta i A. Cvitanić sakupljanjem na području Dalmacije. Veliki dio zbirke ne sadrži podatke o sakupljaču kao ni datum sakupljanja. Zbirka je nastala u periodu od 20‐tih godina XX. stoljeća do kraja 2014. godine. Ključne riječi: zmije, herpetološka zbirka, prirodoslovni muzej u Splitu, Hrvatska SNAKES IN HERPETOLOGICAL COLLECTION OF THE NATURAL HISTORY MUSEUM IN SPLIT, CROATIA S. Mekinić1,3, N. Ževrnja2, J. Boban2, G. Piasevoli1, D. Vladović2 1 Public Institution for the Management of Protected Areas in the County of Split and Dalmatia – „Sea and karst“, Split, Croatia ([email protected]) 2 Natural History Museum and Zoo, Split, Croatia 3 Gata 73, 21253 Gata, Croatia Analysis of the herpetological collection of Natural History Museum in Split found that snakes were represented in the herpetological collection by 183 inventory numbers with 224 samples. In the herpetological collection there are samples of 4 families, 5 subfamilies, 11 genus and 15 snakes species. The most represented samples are from Natracinae and Viperidae family, infact genders Natrix and Vipera. The largest number of samples were collected by Girometta U. and Cvitanić A. collecting at the area od Dalmatia. A large part of the collection does not contain information of collector or collection date. The collection was created in period of 20‐ies of XX. century to the end of 2014. Key words: snakes, herpetological collection, natural history museum in Split, Croatia P‐53 DISCOVERY OF AN AMPHIKINETIC AMPHIBIAN SKULL AND ITS LINKAGE TO NEOTENIC LIFESTYLE N. Natchev1,2, S. Handschuh3, S. Lukanov4, N. Tzankov5, B. Naumov4, I. Werneburg6 1 Department of Integrative Zoology, Vienna University, Vienna, Austria 2 Faculty of Natural Science, Shumen University, Shumen, Bulgaria 3 VetCore Facility for Research, University of Veterinary Medicine Vienna, Vienna, Austria th
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4
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria ([email protected]) 5 National Museum of Natural History, Bulgarian Academy of Sciences, Sofia, Bulgaria 6 Museum für Naturkunde Leibniz‐Institut für Evolutions‐ & Biodiversitätsforschung an der Humboldt‐Universität zu Berlin, Berlin, Germany Among urodeles there is a significant variation in the degree of skull kinetics, with some species possessing a highly kinetic skull during their larval stages, which then ossifies in the course of the ontogenetic development. We used high‐speed cinematography (420 fps) and micro‐computed tomography (μCT) to study the feeding behaviour in the recently described Buresch’s newt Triturus ivanbureschi Arntzen & Wielstra, 2013. In addition to the typical amphibian articulation between skull roof and palatoquadrate associated structures (pleurokinesis) there were also flexible connections between parietals and frontals (mesokinesis) and between parietals and the exocipitals (metakinesis). This morphological pattern is similar to that observed in modern lizards but the combination of pleuro‐, meta‐, and mesokinetic skull articulations found in the Buresch’s newt represents a new kind of amphikinetics not described for any vertebrate to date. The functional analysis of the cranial kinesis in T. ivanbureschi indicates that the advantage of having less rigid skull morphology is to optimize for jaw closing mechanism. We propose that the cranial kinesis in T. ivanbureschi increases the efficiency of fast jaw closure and may represent a neotenic feature retained from the newt larvae. The presence of intracranial mobility may be influenced by specific (yet undetermined) environmental conditions. Key words: Urodela, kinetics, metamorphosis, adaptation, biomechanics. P‐54 'ZAMKE SMRTI' ‐ NEPRIKLADNI GRAĐEVINSKI ZAHVATI U ZAŠTIĆENIM PODRUČJIMA MOGU UZROKOVATI VISOKU SMRTNOST VODOZEMACA I GMAZOVA V. Šoltić1, O. Jovanović2, D. Jelić3 1 Udruga studenata biologije ‐ 'ZOA', Osijek, Hrvatska ([email protected]) 2 Odjel za biologiju, Sveučilište Josipa Jurja Strossmayera, Osijek,Hrvatska 3 Hrvatski institut za biološku raznolikost, Hrvatsko herpetološko društvo, Zagreb, Hrvatska Vodozemci i gmazovi spadaju među globalno najugroženije skupine životinja. Njihov je opstanak danas direktno vezan uz mogućnost prilagodbe na izmijenjena antropogena staništa. No, od presudne važnosti je i naša mogućnost prilagodbe zahvata da budu čim više ekološki prihvatljivi. Ovdje iznosimo problem građevinskih objekata koji zbog svoje neprilagođenosti prirodi uzrokuju vrlo visoku smrtnost jedinki različitih vrsta – 'zamke smrti'. Naše istraživanje obuhvatilo je primjer odvodnog sustava postavljenog na parkiralištu ispred zgrade JUPP Kopački rit u Kopačevu, Hrvatska. Građevinski objekt sastoji se od oko 80 m odvodnih kanala i velikog spremnika za sabirnu vodu (zapremine oko 15 m3). Kanali djeluju kao prepreka životinjama koje tuda prolaze i njima se usmjeravaju u podzemni spremnik. Ovaj efekt je prvi put zabilježen u svibnju 2010. godine kada je slučajnim pregledom u spremniku pronađeno oko 200‐tinjak jedinki vodozemaca od čega je 50% već bilo mrtvo. Tijekom 2015. godine proveli smo praćenje ovog sustava kako bi pokušali kvantificirati negativan utjecaj i mortalitet. Ukupno je iz spremnika izvađeno 245 jedinki, i to šest vrsta vodozemaca i jedna vrsta gmaza, od čega je oko 5% već bilo mrtvo, a preostale su spašene i puštene natrag u prirodu. Da jedinke nisu izvađene iz spremnika, sigurno bi sve stradale. Tijekom travnja i svibnja zbilježeno je da u th
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prosjeku dnevno u spremnik upadne 4,98 jedinki.Trenutno se razmatraju moguće mjere za smanjenje negativnog utjecaja, te generalne smjernice za buduće građevinske zahvate. Ključne riječi: vodozemci, gmazovi, smrtnost, zamka smrti, građevinarstvo, parkiralište 'DEATH TRAPS' – INAPPROPRIATE CONSTRUCTIONS IN PROTECTED AREAS CAN CAUSE HIGH MORTALITY IN AMPHIBIANS AND REPTILES V. Šoltić1, O. Jovanović2, D. Jelić3 1 Biology Student Association ‐ 'ZOA', Osijek, Croatia ([email protected]) 2 Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia 3 Croatian Institute for Biodiversity, Croatian Herpetological Society, Zagreb, Croatia Amphibians and reptiles are among the most threatened groups of animals on our planet. Their future survival is directly connected to their ability to adapt to ever spreading anthropogenic habitats. But it is also important that we make our impact to be as ecologically friendly as possible. Here we describe the problem of urban constructions which cause very high animal mortality when used in natural habitats – called 'death traps'. Our research was focused on parking drainage system built in front of Kopačkirit Nature Park visitor centre in Kopačevo, Croatia. This construction is made out of around 80 meters of collection channels and a large underground tank (volume of around 15 m3). Collection channels act as pitfall traps collecting animals from around parking surface and focusing them to underground tank. This effect was first observed in May 2010 when more than 200 animals were found within the underground tank (around 50% of animals were already dead). In 2015 we started a continuous monitoring on this location in order to quantify the negative effect and mortality. In total we collected 245 individuals, belonging to six amphibian and one reptile species, from which around 5% was already found dead. All animals were removed from the tank during each research occasion and live individuals were released back into nature. If not removed all animals would die eventually. During April and May sampling we calculated that in average 4.98 individuals fall into the tank each day. We give a set of conservation measures that can reduce the mortality and suggest general guidelines for development of more eco‐friendly constructions. Key words: Amphibian, reptile, mortality, construction, death trap, car parking P‐55 SEXUAL DIMORPHISM, DIET AND REPRODUCTION OF THE GRASS SNAKE (Natrix natrix) IN THE REGION OF THE MARSHY‐POND ECOSYSTEM BARDAČA (REPUBLIC OF SRPSKA, BOSNIA AND HERZEGOVINA) G. Šukalo1, S. Đorđević3, Lj. Tomović2,3 1 Faculty of Sciences, University of Banja Luka, Banja Luka, Republic of Srpska, Bosnia and Herzegovina ([email protected]) 2 Institute for Biological Research “Siniša Stanković, University of Belgrade, Belgrade, Serbia 3 Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia In this publication we present the first data concerning ecological characteristics of the grass snake, Natrix natrix, based on the data collected during the capture‐mark‐recapture study in the area of marshy‐pond ecosystem Bardača between 2011 and 2014. We captured, processed th
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and marked 172 adult individuals (96 ♀ : 76 ♂), in which we analysed body weight, seven morphometric characteristics and two meristic traits. Females had larger average values of body weight and of all analysed morphometric traits; however, considered as a ratio of total body length, males had longer tails than females (22.3% compared to 19.3%). Additionally, males had significantly larger numbers of ventral and subcaudal scales. The analysis of diet of the snakes we processed was conducted on the contents spontaneously regurgitated by the animals, while by palpating abdomens of gravid females, we assessed the numbers of growing follicles/eggs they had. We found statistically significant differences in the type of consumed prey among different years, which indicates the grass snake’s dietary plasticity. We also found differences in the diets of the two genders, where females more often consumed adult green frogs (Pelophylax sp.), while males usually consumed fish (especially Pseudorasbora parva). The smallest female with growing follicles had standard body length (SVL) of 62.6 cm, while the smallest male which was observed in mating had a SVL of 48.5 cm. Clutch size varied between 8 and 28. Key words: grass snake, body size, diet, clutch size P‐56 DETECTION OF CONTINUED ANNUAL MORTALITY IN THE VULNERABLE ALPINE SALAMANDER, Salamandra atra prenjensis (MIKŠIĆ, 1969) NOT ASSOCIATED WITH THE PRESENCE OF KNOWN AMPHIBIAN PATHOGENS E. Šunje1,2, F. Pasmans3, Z. Maksimović4, A. Martel3, M. Rifatbefović4 1 Faculty of Natural Science Sarajevo, Sarajevo, Bosnia and Herzegovina ([email protected]) 2 Herpetological Association of Bosnia and Herzegovina: ATRA (BH‐HU: ATRA), Sarajevo, Bosnia and Herzegovina 3 Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium 4 Department of Microbiology, Faculty of Veterinary Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina The emerging disease, chytridiomycosis plays a key role in amphibian declines and species extinctions. Additional agents contributing to this issue are Rana virus (Rv) and a recently discovered fungus: Batrachochytridium salamandrivorans (Bs). Chytridiomycosis’s etiological agent, the fungus Batrachochytrium dendrobatidis (Bd), has been reported on all continents inhabited by amphibians. In Europe it is detected in 17 EU Member states. Occurrence of the disease in Balkan area is almost unknown. In this study we report first results for chytridiomycosis analyzes in Bosnia and Herzegovina (B&H) for caudate endemic Salamandra atra prenjensis. During summer 2013, fieldwork was performed on two B&H Mountains. In total, 145 individuals of S. a. prenjensis were swabbed and screened for Bd infection. Analyzes were performed using quantitative real‐time PCR (qPCR). Results were negative for all individuals. On Mt. Prenj, sick and dead individuals expressing specific symptoms (abnormal posture, cachexia) were found and collected. Besides for Bd, dead animals were additionally screened for Bs and Rv, which showed absence of these infections. Additional histopathology of skin also proved absence of chytridiomycosis. The phenomenon has been regularly registered during several years of fieldwork (2% incidence). Possible explanations of its occurrence are presented in this paper. th
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Key words: amphibian disease, Bosnia and Herzegovina (B&H), mountain ecosystem P‐57 PRELIMINARY ASSESSMENT OF THE LACERTID SKULL MODULARITY – A CASE STUDY OF Podarcis muralis A. Urošević1, K. Ljubisavljević1, A. Ivanović2 1 University of Belgrade, Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia ([email protected]) 2 Institute of Zoology, Faculty of Biology, University of Belgrade, Belgrade, Serbia The concepts of modularity and morphological integration have gained prominent attention in the evolutionary biology in recent years. Here we present a study of dorsal and ventral cranium modularity within the single species – European wall lizard (Podarcis muralis Laurenti, 1768), on a sample of 25 neonates, 26 females and 26 males. For the dorsal cranium, we evaluated three hypotheses which were already suggested for lizard dorsal cranium: mammalian hypothesis, ‘Anolis’ hypothesis and tripartite hypothesis. For the ventral cranium, we tested two hypotheses: the developmental hypothesis (braincase vs. dermatocranium) and the functional hypothesis (jaw and palate vs. posterior cranium). Since the allometry was significant in the whole sample, we did our tests of modularity hypotheses on size corrected dataset. For the dorsal cranium only ‘Anolis’ hypothesis was supported for adult females and males, but not for the neonates. For the ventral cranium, only the functional hypothesis was supported for adult females and males, but not for neonates. Our conclusions would be that there is an onthogenetic shift in the pattern of skull integration, from neonates which have highly integrated skull, to adults in which functional constraints lead to distinct functional modularity. Key words: cranium modularity, developmental hypothesis, European wall lizard, functional hypothesis P‐58 RIĐOVKA, Vipera berus (SQUAMATA, VIPERIDAE) U HRVATSKOJ: POPULACIJSKA EKOLOGIJA, ODABIR MIKROSTANIŠTA I TERMOREGULACIJA I. Vilaj, B. Lauš, D. Jelić Hrvatsko herpetološko društvo HYLA, Zagreb, Hrvatska ([email protected]) Riđovka, Vipera berus (Linnaeus, 1785) je najšire, a ujedno i najsjevernije rasprostranjena terestrička vrsta zmije u svijetu. U Hrvatskoj nalazimo dvije podvrste u tri odvojene populacije. Terenska istraživanja provedena su na dva različita lokaliteta u Hrvatskoj: Okuje (Turopolje, dolina rijeke Save; V. b. bosniensis) i Begovo Razdolje (Gorski kotar; V. b. berus). Monitoring vrste proveden je Capture – Mark – Recapture metodom tokom četiri godine u Okuju (2009.‐ 2012.), odnosno tri godine u Begovom Razdolju (2009.‐2011.). Cilj istraživanja bio je utvrditi postoje li interpopulacijske i intrapopulacijske sličnosti i razlike u odabiru mikrostaništa, termoregulaciji, dnevnoj i godišnjoj aktivnosti, spolnoj strukturi te analiziranim morfometrijskim i merističkim karakteristikama. Analizirani uzorak obuhvaćao je ukupno 106 jedinki (50 iz Begovog Razdolja, 56 iz Okuja). Utvrđeno je da postoje statistički značajne razlike u duljini i visini glave te tjelesnoj masi jedinki. Jedinke riđovke u Okuju preferiraju zatvoreni tip, a jedinke u Begovom Razdolju otvoreni tip mikrostaništa. U obje populacije postoji razlika u th
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spolnoj strukturi te termoregulaciji prema spolu i tipu obojenja. U Okuju riđovke počinju svoju aktivnost u mjesecu ožujku, a u Begovom Razdolju u mjesecu lipnju. Ključne riječi: Capture – Mark – Recapture metoda, Vipera berus, ekološke preferencije, spolna struktura, merističke karakteristike COMMON ADDER, Vipera berus (SQUAMATA, VIPERIDAE) IN CROATIA: POPULATION ECOLOGY, MICROHABITAT PREFERENCES AND THERMOREGULATION I. Vilaj, B. Lauš, D. Jelić Croatian Herpetological Society HYLA, Zagreb, Hrvatska ([email protected]) The Adder, Vipera berus (Linnaeus, 1785) is the most widespread and also the northernmost distributed terrestrial snake species in the world. In Croatia, there are two subspecies of the adder in three separate populations. Field work was conducted on two different localities in Croatia: Okuje (Turopolje, river Sava Valley; Vipera berus bosniensis) and Begovo Razdolje (Gorski kotar; Vipera berus berus). The monitoring was conducted by Capture – Mark – Recapture method during a four year period in Okuje (2009 – 2012), and a three year period in Begovo Razdolje (2009 – 2011). The main goals of this study were to determine if there are intrapopulation or interpopulation similarities or differences in microhabitat preferences, thermoregulation, daily or yearly activity period, gender structure of population and meristic characteristics. We collected 106 adders (50 from Begovo Razdolje and 56 from Okuje). The analyses have shown that there are statistically significant differences between the two populations in head length, head height and weight. The individuals from Okuje prefer a closed type of microhabitat, unlike individuals from Begovo Razdolje that prefer an opened type of microhabitat. In both populations, there are differences in the gender structure, and thermoregulation which is sex and colour morph dependent. In Okuje, Adders begin their activity period in March as opposed to individuals from Begovo Razdolje whose activity period begins in June. Key words: Capture – Mark – Recapture, activity period, gender structure, meristic characteristics. P‐59 GMAZOVI I VODOZEMCI VUGROVEČKE OKOLICE M. Zadravec Hrvatsko herpetološko društvo HYLA, Zagreb, Hrvatska ([email protected]) Faunistička istraživanja gmazova i vodozemaca u Hrvatskoj nisu česta, pogotovo u kontinentalnom dijelu. U ovom radu su dani nalazi 11‐godišnjeg inventariziranja gmazova i vodozemaca na području sela Vugrovca i okolice, nedaleko od Zagreba, provedenog od 2004. do 2014. godine. Dio istraživanog područja spada pod novo Natura 2000 područje 'Vejalnica i Krč', a dio u Značajni krajobraz 'Goranec'. Objema upravlja Javna ustanova 'Maksimir'. Gmazovi su lovljeni rukom, ponekad uz uporabu zaštitnih rukavica i kuka za zmije. Vodozemci su lovljeni rukama i ručnim mrežama. Ulovljene jedinke su fotografirane i neozlijeđene puštene na mjesto ulova u najkraćem mogućem roku. Zabilježena je prisutnost 11 vrsta gmazova i sedam vrsta vodozemaca. Među gmazovima je prisutna jedna unešena vrsta – čančara, Testudo hermanni Gmelin, 1789, koja ne obitava prirodno u ovom dijelu Hrvatske. Nalaz žutog mukača, Bombina variegata (Linnaeus, 1758), je od posebnog značaja jer je na Dodacima II i IV Direktive th
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o staništima Europske komisije. Rezultati ovog istraživanja daju doprinos poznavanju prisutnosti i raširenosti gmazova i vodozemaca u zagrebačkoj okolici te na Natura 2000 području 'Vejalnica i Krč' i u Značajnom krajobrazu 'Goranec'. Prikupljeni podaci mogu pomoći djelatnicima Javne ustanove 'Maksimir' i prilikom upravljanja tim područjem. Ključne riječi: herpetofauna, Natura 2000, 'Vejalnica i Krč' REPTILES AND AMPHIBIANS OF THE VUGROVEC AREA M. Zadravec Croatian Herpetological Society HYLA, Zagreb, Croatia ([email protected]) Faunistic research of reptiles and amphibians in Croatia are not so commonplace, especially in the continental part. Here I present the results of an 11‐year inventarisation study of reptiles and amphibians in the village of Vugrovec and its environs, near Zagreb, conducted from 2004 till 2014. A part of the research area falls under the new Natura 2000 site 'Vejalnica and Krč' and another one under the Significant landscape 'Goranec'. Both are being administered by the Public institution 'Maksimir'. Reptiles were caught by hand, sometimes with the use of special gloves and snake hooks. Amphibians were caught by hand and handheld nets. The caught individuals were photographed and released afterwards at the same spot in the shortest time possible. A total of 11 reptile and seven amphibian species were recorded. One of the reptile species is introduced – the Hermann's tortoise, Testudo hermanni (Gmelin, 1789). It does not occur naturally in this part of Croatia. Of special importance is the finding of Bombina variegata (Linnaeus, 1758), which is on Annexes II and IV of the European Comission's Habitats Directive. The results of this research contribute to the knowledge about the occurence and distribution of reptiles and amphibians in the Zagreb area, and within the Natura 2000 site 'Vejalnica and Krč' and the Significant landscape 'Goranec'. They can also be of use to the employees of the Public institution 'Maksimir' for the creation of management plans for the two sites. Key words: herpetofauna, Natura 2000, 'Vejalnica and Krč' P‐60 GMAZOVI I VODOZEMCI KAMENOLOMA BIZEK M. Zadravec1,2, R. Cesarec3, Z. Tadić1 1 Zavod za animalnu fiziologiju, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska 2 Hrvatsko herpetološko društvo HYLA, Zagreb, Hrvatska ([email protected]) 3 Treščak 6, Zagreb, Hrvatska Na ovom posteru predstavljeni su rezultati faunističkog istraživanja gmazova i vodozemaca u neaktivnom kamenolomu Bizek, od 2008. do 2014. godine. Na istraživanom području su raznolika staništa: mješovita šuma bukve, hrasta i graba okružuje kamenolom sa sjeverne, istočne i zapadne strane. Termofilni elementi dominiraju na zapadnoj strani, dok na južnoj šuma prelazi u dvorišta i stambene objekte. Dva stalna vodotoka i jedna lokva su također prisutni, uz nekoliko povremenih vodotokova i lokvica. Jedinke obiju skupina su lovljene rukom, a u nekim slučajevima koristilo se štap s omčicom za guštere, kuke i rukavice za zmije te mreža za vodozemce. Pronađeno je sedam vrsta gmazova i sedam vrsta vodozemaca. Sve pronađene vrste su već poznate iz zagrebačke okolice. Zbog složenosti i nemogućnosti određivanja zelenih žaba (Pelophylax spp.) do vrste na temelju vanjskog izgleda, sve nađene jedinke ostavljene su na razini th
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svojte. Od posebnog značaja je nalaz žutog mukača, Bombina variegata (Linnaeus, 1758), koji je na Dodacima II i IV Direktive o staništima Europske komisije. Rezultati ovog istraživanja daju doprinos poznavanju prisutnosti i rasprostranjenosti vrsta gmazova i vodozemaca na samome rubu grada Zagreba i unutar Parka prirode Medvednica. Ključne riječi: herpetofauna, Natura 2000, Park prirode Medvednica REPTILES AND AMPHIBIANS OF THE BIZEK QUARRY M. Zadravec1,2, R. Cesarec3, Z. Tadić1 1 Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia 2 Croatian Herpetological Society HYLA, Zagreb, Croatia ([email protected]) 3 Treščak 6, Zagreb, Croatia This was a study of reptiles and amphibians which lasted from 2008 till 2014 in the inactive Bizek quarry, where we collected information on the presence of species belonging to those two groups. A variety of habitats occur in the quarry: a mixed deciduous forest of Quercus sp., Fagus sylvatica and Carpinus betulus surrounds the quarry to the north, east and west. Thermophilic elements are predominant on the western side, while on the southern side the forest gives way to yards and housing. Two permanent streams and a pond are also present, along with some intermittent streams and puddles. We caught animals by hand and, in some cases, we also used a stick with a noose for lizards, gloves and hooks for snakes, and a net for the amphibians. We found seven reptile and seven amphibian species in the quarry, all of which are already known from the surroundings of Zagreb. Frogs belonging to the genus Pelophylax Fitzinger, 1843 were identified down to the genus level only, because of the complexity of the determination and the inability to reliably identify them on the basis of external morphology. The finding of Bombina variegata (Linnaeus, 1758), which is on Annexes II and IV of the European Comission's Habitats Directive, is of special significance. The results of this study contribute to the knowledge of the occurrence and distribution of suburban reptiles and amphibians within the Nature Park Medvednica. Key words: herpetofauna, Natura 2000, Nature Park Medvednica P‐61 NEW REVISION OF THE CHECKLIST OF AMPHIBIAN FAUNA IN BOSNIA AND HERZEGOVINA A. Zimić1, D. Jelić2, S. Lelo3, M. Čengić4 1 Herpetological Association in Bosnia and Herzegovina 'Atra', Sarajevo, Bosnia and Herzegovina ([email protected]) 2 Croatian Herpetological Society HYLA, Zagreb, Croatia 3 Department of Biology, Faculty of Science and Mathematics, Sarajevo, Bosnia and Herzegovina 4 Department of physical geography and ecosystem science, Lund University, Lund, Sweden Research of batrahofauna of Bosnia and Herzegovina (B&H) has a long tradition. It can be traced back to the O. Möellendorf PhD dissertation in 1873. The batrachological investigations in B&H could be divide on four periods: (1) The period of Ottoman Empire/Austro‐Hungarian Monarchy (until 1918), (2) The period of the Kingdom of Yugoslavia (1918‐1943), (3) The period of the Socialist Federal Republic of Yugoslavia (1943‐1992) and (4) The period of independent B&H. The main aim of this paper is to give an update of biosystematic information and to th
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present the new precise distribution maps for all amphibian species in B&H. With new information (literature and field data) we found significant differences comparative to the previous reviews. Two new amphibian species were found, so there is evidence that 21 amphibian species (including one klepton) occurs in B&H. Authors have confirmed existence of all 21 species. The most common species are Pelophylax ridibundus and Bombina variegata and the rarest ones are Triturus carnifex, Triturus dobrogicus, Pelobates fuscus, Salamandra atra and Triturus macedonicus, with just several findings. With 65% species of batrahofauna of Balkan penninsula and two subendemic taxa (Proteus anguinus and Salamandra atra prenjensis) B&H can be considered as important area of amphibian biodiversity in Southern Europe. New expected (sub)species and some biosystematic problems are also discussed in this paper. Key words: distribution, biosystematics, batrachology, Bosnia and Herzegovina BIOLOGIJA MORA MARINE BIOLOGY P‐62 UVIDI O EKOLOGIJI PREHRANE SREDOZEMNE MEDVJEDICE J.Antolović1, E.Coppola2, L.Bundone2, S.Žalac1, D.Škalić1, V.Stinga Perusco1, G.Hernandez Milan3 1 Grupa sredozemna medvjedica, Udruga za istraživanje i zaštitu prirode, Zagreb, Hrvatska ([email protected]‐com.hr) 2 Gruppo Foca Monaca, Rim, Italija 3 University College Cork, Western Road, Cork, Irska Sredozemna medvjedica je vrsta kritično ugroženih morskih sisavaca čije stanište i ponašanje su u velikom dijelu i dalje nepoznati u Mediteranu i Jadranu. U ovoj studiji smo predstavili neke uvide o prehrani ovog grabežljivca. Uzorci izmeta iz sjevernog Jadrana (Istra‐Hrvatska, n=24, 2010.‐2014.) prikupljeni su i analizirani za istraživanje o prehrani. U svim uzorcima pronađene su samo riblje kosti i ljuske. Većina plijena (36%) sastoji se od riba Ljuskavki, od čega su najčešći identificirani plijen bili ušata i trlja, tj. barbun. Ostale ostatke je moguće samo klasificirati kao Grgečke zbog stupnja probave kostiju. Iako je studija provedena s ograničenom veličinom uzorka, možemo istaknuti važnost ove informacije za ugroženog sisavca kao što je sredozemna medvjedica, te korištenje izmeta po prvi puta za ovu vrstu. Ključne riječi: sredozemna medvjedica, uzorci izmeta, prehrana INSIGHTS ON THE FORAGING ECOLOGY OF THE MEDITERRANEAN MONK SEAL J. Antolović1, E. Coppola2, L. Bundone2, S. Žalac1, D. Škalić1, V. Stinga Perusco1, G. Hernandez Milan3 1 The Mediterranean Monk Seal Group, Association for Wildlife Research and Protection, Zagreb, Croatia ([email protected]‐com.hr) 2 Gruppo Foca Monaca, Roma, Italy 3 University College Cork, Western Road, Cork, Ireland The Mediterranean monk seal is a critically endangered marine mammal species whose habitat and behavior are in great part still unknown in the Mediterranean and Adriatic. In this study we present some insights about the foraging of this predator. Scat samples from north Adriatic th
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(Croatia, n=24, 2010‐2014) were collected and analyzed for dietary investigations. In all samples, only fish bones and scales were recovered. Most of the preys items (36%) consist on Sparidae fish were saddle sea bream and surmullet were the most common identified prey. Other remains were only possible to classify as Perciforms due to the digestion degree of the bones. Although the study was carried out with a limited sample size, we underline the importance of this information for an endanger mammal such as the Mediterranean Monk Seal, and the use of scats for the first time in this species. Key words: The Mediterranean monk seal, Scat samples, The foraging P‐63 Brooksia lacromae sp. nov. (Tunicata, Thaliacea), NOVA VRSTA DVOOTVORKE U JADRANSKOM MORU M. Batistić, R. Garić Sveučilište u Dubrovniku, Institut za more i priobalje, Dubrovnik, Hrvatska ([email protected]) Brooksia lacromae sp. nov. Garić & Batistić je opisana iz zooplanktonskog materijala sakupljenog na stalnoj postaji u južnom Jadranu u jesen 2014. Vrsta se morforloški značajno razlikuje od jedine dvije poznate vrste istog roda, B. rostrata i B. berneri. Genetička analiza bazirana na genu za 18S rRNA potvrdila je različitost vrste B. lacromae od vrste B. rostrata (p‐ vrijednost između sekvenci je bila 1,5%). U istim uzorcima pronađena je i atlantska vrsta repnjaka Fritillaria helenae pa možemo pretpostaviti da su obje vrste atlantskog/zapadnomediteranskog podrijetla te su mogući indikatori atlantsko/zapadnomediteranske struje. Ključne riječi: Thaliacea, nova vrsta, Brooksia lacromae, Jadransko more Brooksia lacromae sp. nov. (Tunicata, Thaliacea), A NEW SPECIES OF SALP FROM THE ADRIATIC SEA M. Batistić, R. Garić Institute for marine and coastal research, University of Dubrovnik, Croatia ([email protected]) Brooksia lacromae sp. nov. Garić & Batistić was described from zooplankton material collected at a marine monitoring station in the South Adriatic in autumn 2014. This new species is morphologically markedly different from B. rostrata and B. berneri, so far the only two species in the genus Brooksia. Genetic analysis based on 18S rRNA gene confirmed distinctness (p‐ value between sequences 1.5%) of B. lacromae from B. rostrata. Occurrence of B. lacromae with an Atlantic appendicularian, Fritillaria helenae, in the same samples suggests its Atlantic or Western Mediterranean origin and its potential for being an indicator species of Atlantic/Western Mediterranean waters. Key words: Thaliacea, new species, Brooksia lacromae, Adriatic Sea th
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P‐64 ZBIRKA RAKOVA PRIRODOSLOVNOG MUZEJA U SPLITU J. Boban, D. Vladović Prirodoslovni muzej i zoološki vrt, Split, Hrvatska ([email protected]) Zbirku rakova u Prirodoslovnom muzeju u Splitu započeo je skupljati profesor Umberto Girometta te njezin početak nastajanja možemo smjestiti u 20‐te godine dvadesetog stoljeća. Zbirka se temelji na uzorcima osam sakupljača. Zbirka rakova Prirodoslovnog muzeja u Splitu broji 409 primjeraka raspoređenih u 124 inventarna broja. Analizom je utvrđeno da zbirka sadrži 70 morskih vrsta rakova unutar 53 roda, 40 porodica i 6 redova. Važno je istaknuti 89 primjeraka koji su sakupljeni u Jadranu na znanstvenim ekspedicijama Instituta za oceanografiju i ribarstvo: AdriaMed i MEDITS. Ostali primjerci su sakupljeni u osobnim terenskim istraživanjima sakupljača, a dva primjerka su darovana. Ključne riječi: Zbirka rakova, rakovi, Prirodoslovni muzej, Umberto Girometta NATURAL HISTORY MUSEUM CRUSTACEAN COLLECTION IN SPLIT J. Boban, D. Vladović Natural History Museum and Zoo, Split, Croatia ([email protected]) Crustacean collection of the Natural History Museum in Split began to collect Professor Umberto Girometta and its origin can be dated in the early 1920‐ies. The collection is based on samples from eight collectors. The collection of the Natural Museum in Split has 409 copies distributed to 124 inventory numbers. The analysis shows that the collection contains 70 marine species of crustaceans within 53 genus, 40 families and 6 orders. It is important to highlight 89 specimens that were collected in the Adriatic Sea on the scientific expeditions of the Institute of Oceanography and Fisheries: AdriaMed and MEDITS. Other specimens were collected in the personal field researches of collectors, and two copies were donated. Key words: Crustacean collection, Crustaceans, Natural History Museum, Umberto Girometta P‐65 MORFOLOGIJA I FILOGENIJA RODA Bacteriastrum (BACILLARIOPHYTA) U JADRANSKOM MORU S. Bosak1, L. Šupraha2, D. Nanjappa3, W. Kooistra3, D. Sarno3 1 Botanički zavod, Biološki odsjek, Prirodoslovno‐matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska ([email protected]) 2 Sveučilište u Uppsali, Uppsala, Švedska 3 Stazione Zoologica Anton Dohrn, Napulj, Italija Četiri kolonijalne vrste planktonskih dijatomeja roda Bacteriastrum – B. furcatum, B. hyalinum, B. jadranum i B. mediterraneum analizirane su u uzorcima iz Jadranskog mora te su iz njih uzgojene kulture u laboratoriju. Opisana je morfologija i ultrastruktura frustula koristeći svjetlosni i elektronski mikroskop, te su dobivene sekvence velike podjedinice (LSU) rDNA. Opis vrste B. jadranum je dopunjen i ispravljen na osnovi otkrića novog i jedinstvenog načina formiranja kolonija kod te vrste koji uključuje povezivanje stanica u lance pomoću organske tvari. Dopunjeni su opisi dvije od tri preostale vrste; vegetativne stanice vrste B. hyalinum posjeduju kratke spinule th
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u obliku slova Y na spojenim dijelovima seta i izdanke na valve u obliku slova T, dok mirujuće spore posjeduju nepravilnu površinu vanjskog dijela omotača primarne valve. Za vrstu B. furcatum na đeno je da posjeduje specifičan raspored pora na površini valve. Filogenetski rezultati grupirali su LSU sekvence četiri vrste u dvije sestrinske grane; na prvoj se nalaze B. furcatum (odjel Sagittata) i B. hyalinum (odjel Isomorpha) dok su na drugoj smješteni B. jadranum (odjel Isomorpha) i B. mediterraneum (odjel Sagittata). Možemo zaključiti da je rod Bacteriastrum monofiletski rod, međutim odjeli ne dijele monofiletsko porijeklo te njihove pretpostavljene određujuće taksonomske značajke: izomorfne terminalne sete kod vrsta koje pripadaju odjelu Isomorpha te dimorfne kod vrsta odjela Sagitatta nisu sinapomorfije. Ključne riječi: Planktonske dijatomeje, Bacteriastrum, Jadransko more MORPHOLOGY AND PHYLOGENY OF THE GENUS Bacteriastrum (BACILLARIOPHYTA) IN THE ADRIATIC SEA S. Bosak1, L. Šupraha2, D. Nanjappa3, W. Kooistra3, D. Sarno3 1 Division of Botany, Department of Biology, Zagreb, Croatia ([email protected]) 2 Paleobiology/Department of Earth Sciences, Uppsala University, Uppsala, Sweden 3 Stazione Zoologica Anton DohrnNaples, Italy Four colonial species of the multipolar centric diatom genus Bacteriastrum: B. furcatum, B. hyalinum, B. jadranum and B. mediterraneum were investigated using field samples from the Adriatic Sea and cultured strains obtained from these samples. Morphology and frustule ultrastructure were examined using light and electron microscopy, and nuclear‐encoded large‐ subunit (LSU) rDNA sequences were obtained from the strains. The description of B. jadranum has been emended based on a novel mechanism of colony formation in which cells are joined through an organic cell jacket which holds together cells in chains. Descriptions for two of the three other species were supplemented; vegetative cells of B. hyalinum have short Y spinules on the fused part of the setae and T‐shaped outgrowths, and the spores of this species possess a granular surface on the exterior side of t he primary valve mantle. Specific for B. furcatum is the location of pores scattered around the setae bases. Phylogenetic results grouped the sequences of the four species in a clade in which B. furcatum (section Sagittata) and B. hyalinum (section Isomorpha) formed a clade as sister to a clade with B. jadranum (section Isomorpha) and B. mediterraneum (section Sagittata). Apparently, neither section was monophyletic, and the supposed defining features of isomorphic terminal setae in Isomorpha and dimorphic ones in Sagittata were not synapomorphies. Nonetheless, the genus Bacteriastrum itself remained monophyletic. Key words: planktonic diatoms, Bacteriastrum, Adriatic Sea P‐66 SASTAV MAKROZOOBENTOSKIH BESKRALJEŠNJAKA U OBRAŠTAJNIM ZAJEDNICAMA U LUCI SPLIT (HRVATSKA, JADRANSKO MORE) M. Despalatović, I. Cvitković, A. Žuljević Laboratorij za bentos, Institut za oceanografiju i ribarstvo, Split, Hrvatska ([email protected]) Istraživanje obraštajnih zajednica u luci Split, važnoj hrvatskoj luci za međunarodni pomorski promet, obavljeno je na dva teretna terminala (Sjeverna luka i Sveti Kajo) u proljeće i jesen th
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2014. godine. Korištena je metodologija uzorkovanja koja se bazira na CRIMP protokolu, a na pojedinom terminalu uzorci su sakupljani na tri transekta, međusobno udaljena 15 m, s betonskih vertikalnih zidova, s dubina od 0,5 m, 3 m i 7 m, te iz sedimenta. Ukupno su zabilježene 132 vrste makrozoobentoskih beskralješnjaka, 84 na terminalu Sjeverna luka i 108 na terminalu Sveti Kajo. Zabilježene su dvije alohtone vrste: morski puž Siphonaria pectinata (Linnaeus, 1758), prvi put zabilježen u akvatoriju grada Splita 2003. godine; te kolonijalni kameni koralj Oculina patagonica de Angelis, 1908, prvi put zabilježen u luci Split 2011. godine. Ključne riječi: obraštajna zajednica, Siphonaria pectinata, Oculina patagonica, luka Split, Jadransko more COMPOSITION OF MACROZOOBENTHIC INVERTEBRATES IN FOULING COMMUNITIES IN THE HARBOUR OF SPLIT (CROATIA, ADRIATIC SEA) M. Despalatović, I. Cvitković, A. Žuljević Laboratory for benthos, Institute of Oceanography and Fisheries, Split, Croatia ([email protected]) Investigation of fouling communities in harbour of Split, important Croatian harbour for international maritime transport, was performed on two cargo terminals (Sjeverna luka and Sveti Kajo) in spring and autumn in 2014. Sampling methodology was based on CRIMP protocol, and samples were collected on each terminal from three transects, 15 m apart from each other, from concrete vertical walls, at depths of 0.5 m, 3 m and 7 m, and from sediment. Total of 132 species of macrozoobenthic invertebrates was recorded, 84 in Sjeverna luka terminal and 108 in Sveti Kajo terminal. Two non‐indigenous species were recorded: marine gastropod Siphonaria pectinata (Linnaeus, 1758), for the first time recorded in Split city aquatorium in 2003; and colonial stony coral Oculina patagonica de Angelis, 1908, for the first time recorded in harbour of Split in 2011. Key words: fouling community, Siphonaria pectinata, Oculina patagonica, harbour Split, Adriatic Sea P‐67 TAKSONOMSKI SASTAV MORSKIH BENTOSKIH DIJATOMEJA I NJEGOV ODNOS S GLAVNIM EKOLOŠKIM ČIMBENICIMA U BOSNI I HERCEGOVINI N. Jasprica1, D. Hafner2, A. Car1, T. Kapetanović3 1 Laboratorij za ekologiju planktona i populacijsku genetiku, Institut za more i priobalje, Sveučilište u Dubrovniku, Dubrovnik, Hrvatska ([email protected]) 2 Bartulovići 4, Blace, Hrvatska 3 Fond za zaštitu okoliša Federacije Bosne i Hercegovine, Sarajevo, Bosna i Hercegovina Na istočnojadranskoj obali postoji svega nekoliko studija o morskim bentoskim dijatomejama, koje su uglavnom bile usmjerene na istraživanje taksonomije i ekologije perifitona u hrvatskim estuarijima i albanskim slanim močvarama. Morske planktonske i bentoske dijatomeje do sada nisu nikada istraživane u Bosni i Hercegovini. Ova studija bila je dio većeg istraživačkog programa s ciljem utvrđivanja bioraznolikosti te funkcioniranja ekosustava u pograničnim obalnim vodama između Hrvatske i Bosne i Hercegovine. Program je uključivao algološka, zoološka i hidrografska istraživanja. U radu se prikazuju taksonomski sastav i sezonalnost morskih bentoskih dijatomeja u odnosu na osnovne ekološke parametre (temperatura, th
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salinitet, gustoća) u Neumskom zaljevu u Bosni i Hercegovini. Ključne riječi: Neumski zaljev, bentos, dijatomeje, istočni Jadran, SI Sredozemlje TAXONOMIC COMPOSITION OF MARINE BENTHIC DIATOMS IN THE RELATION TO SOME KEY ENVIRONMENTAL VARIABLES IN BOSNIA AND HERZEGOVINA N. Jasprica1, D. Hafner2, A. Car1, T. Kapetanović3 1 Laboratory of Plankton Ecology and Population Genetics, Institute for Marine and Coastal Research, University of Dubrovnik, Dubrovnik, Croatia ([email protected]) 2 Bartulovići 4, Blace, Croatia 3 Environmental Fund of the Federation of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina In the eastern Adriatic, there are only a few studies on benthic diatoms mostly focused on ecology and taxonomy of periphytic diatoms in the Croatian estuaries or Albanian coastal wetlands. Until now, both planktonic and benthic marine diatoms have never been studied in Bosnia and Herzegovina. This study forms part of a research program investigating the biodiversity and ecosystem functioning in the border marine coastal waters between the two countries – Croatia and Bosnia and Herzegovina. The program includes phycological, zoological and hydrographical research. This paper considers the taxonomic composition and seasonality of marine benthic diatoms in the relation to some key environmental variables (temperature, salinity and density) in the Bay of Neum in Bosnia and Herzegovina on the south‐eastern Adriatic coast. Key words: Neum Bay, benthos, diatoms, eastern Adriatic, NE Mediterranean P‐68 DUBINSKA RASPROSTRANJENOST KORALJA U KORALIGENSKOJ BIOCENOZI ISTOČNOG JADRANA P. Kružić1, P. Rodić2, A. Popijač3, K. Vučković1, P. Ankon1, V. Matas1, K. Cetinić1, S. Sviben1, A. Kovačev1, N. Moraj1 1 Zoologijski zavod, Prirodoslovno‐matematički fakultet, Zagreb, Hrvatska ([email protected]) 2 Državni zavod za zaštitu prirode, Zagreb, Hrvatska 3 Oikon d.o.o. ‐ Institut za primijenjenu ekologiju, Zagreb, Hrvatska Jedan od glavnih problema vezanih uz spoznaje o koraligenskoj zajednici u Jadranskom moru je nedostatak podataka o dubinskoj rasprostranjenosti pojedinih vrsta. Koraligen se smatra jednim od glavnih 'vrućih točaka' biološke raznolikosti u Sredozemnom moru. Karakterizira ga i velik broj ugroženih dugoživućih vrsta (poput koralja), koje su vrlo osjetljive na antropogene promjene, pogotovo zbog sporog rasta i neizvjesnog oporavka. Početna dubina koraligenske zajednice ovisi o količini svjetlosti koja dopire do morskog dna. Uz smanjenu količinu svjetlosti (dovoljne za veći razvoj crvenih alga (red Corallinales)), ponegdje početak koraligena može biti vrlo plitko. U Jadranskom moru, na podmorskim strmcima, najplića početna dubina za koraligensku biocenozu je oko 20 metara. Raznolikost vrsta koralja, vezano uz dubinsku rasprostranjenost, ovisna je o nekoliko ekoloških čimbenika (morske struje, količina svjetlosti, temperatura i sedimentacija). Najčešće vrste koralja u ovoj biocenozi su Parazoanthus th
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axinellae, Epizoanthus arenaceus, Alcyonium coralloides, Alcyonium acaule, Paramuricea clavata, Eunicella singularis, E. cavolini, Leptopsammia pruvoti, Hoplangia durotrix, Caryophyllia inornata, C. smithii i Madracis pharensis. Crveni koralj (Corallium rubrum) komercijalno je iskorištavan u gotovo svim dijelovima istočnog Jadrana, te su njegove populacije znatno smanjene, pogotovo u plitkom koraligenu. Ključne riječi: Koralji, koraligen, Jadransko more DEPTH DISTRIBUTION OF CORALS IN THE CORALLIGENOUS OF THE EASTERN ADRIATIC SEA P. Kružić1, P. Rodić2, A. Popijač3, K. Vučković1, P. Ankon1, V. Matas1, K. Cetinić1, S. Sviben1, A. Kovačev1, N. Moraj1 1 Department of Biology, Faculty of Science, Zagreb University, Zagreb, Croatia ([email protected]) 2 The State Institute for Nature Protection, Zagreb, Croatia 3 Oikon Ltd, Institute for applied ecology, Zagreb, Croatia
One of the major gaps concerning the current state of knowledge of coralligenous community in the Adriatic Sea is the scarcity of information on species bathymetrical distribution. The coralligenous is considered as one of the most important 'hot spots' of species diversity in the Mediterranean. It is also characterized by harbouring a great number of endangered species, which are long‐lived organisms, whose slow growth and recruitment rates make them very sensitive to disturbances. The minimal depth for the formation of coralligenous concretions depends on the amount of irradiance reaching the sea bottom. Coralligenous concretions can appear in very shallow waters if light conditions are dim enough to allow a great development of coralline algae. In the Adriatic Sea, on the vertical slopes, the minimal depth reaches about 20 meters. Differences among species of corals in tolerances to several physical factors (currents, light, temperature and sedimentation) are thought to determine the upper and lower limits of species depth distributions. The most common coral species are Parazoanthus axinellae, Epizoanthus arenaceus, Alcyonium coralloides, Alcyonium acaule, Paramuricea clavata, Eunicella singularis, E. cavolini, Leptopsammia pruvoti, Hoplangia durotrix, Caryophyllia inornata, C. smithii and Madracis pharensis. Populations of red coral (Corallium rubrum) declined in most areas in the Adriatic Sea, particularly in shallow coralligenous.
Key words: Corals, Coralligenous, Adriatic Sea
P‐69 TROFIČKE INTERAKCIJE U ZAŠTIĆENIM MORSKIM PODRUČJIMA: ULOGA STRANIH INVAZIVNIH VRSTA
M. Marić1, M. De Troch2, S. Olenin1 1 Sveučilište u Klaipedi, Klaipeda, Litva ([email protected]) 2 Sveučilište u Gentu, Biološki odsjek, Ghent, Belgija
Cilj ovog istraživanja je procjena utjecaja invazivnih stranih vrsta (zelene alge Caulerpa cylindracea, crvene alge Asparagopsis sp., desetonožnog raka Percnon gibbesi i puža stražnjoškržnjaka Aplysia dactylomela) u zaštićenom morskom području Pelagijski otoci. Analizom stabilnih izotopa bentičkih organizama kvantificirane su trofičke interakcije između alohtonih i autohtonih morskih vrsta. Usporedbom širina izotopskih niša i modela miješanja izotopa temeljenih na Bayesovoj statistici, istražili smo preklapa li se izotopska niša herbivornih vrsta P. th
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gibbesi i A. dactylomela s nišama autohtonih herbivora i izračunali udio različitih vrsta alga u prehrani stranih i autohtonih vrsta. Rezultati su pokazali da je udio C. cylindracea između 9% i 22%, a najveći je u prehrani P. gibbesi i A. dactylomela (16% i 22%). Ovo istraživanje pokazalo je da invazivne strane alge povećavaju raznolikost raspoloživog plijena i vjerojatno olakšavaju širenje drugih stranih vrsta. Iako strane vrste nisu u izravnoj kompeticiji s autohtonim ježincima Paracentrotus lividus i Arbacia lixula, moguća posljedica njihove velike brojnosti je smanjenje energije dostupne organizmima na višem trofičkom nivou u hranidbenoj mreži. Ključne riječi: strane invazivne vrste, trofičke interakcije, zaštićeno morsko područje, Sredozemno more TROPHIC INTERACTIONS IN MARINE PROTECED AREAS: THE ROLE OF INVASIVE ALIEN SPECIES M. Marić1, M. De Troch2, S. Olenin1 1 Klaipeda University, Klaipeda, Lithuania ([email protected]) 2 Ghent University, Department of Biology, Ghent University, Ghent, Belgium The objective of this study was to evaluate the effects of invasive alien species (green algae Caulerpa cylindracea, red algae Asparagopsis sp., herbivorous crab Percnon gibbesi and sea hare Aplysia dactylomela) in MPA Pelagie Islands. Here, we present the results of the stable isotope analysis of benthic organisms in order to characterise trophic interactions between native and alien species. Using comparisons of species’ isotopic niche widths and Bayesian mixing models, we investigated whether the diet of alien herbivores overlapped with those of native species and quantified the food source contribution to diets of native and alien consumers. Mixing models showed that contribution of C. cylindracea is between 9% and 22%. Highest contributions are those in diets of P. gibbesi and A. dactylomela (16% and 22%, respectively). Results indicated that invasion of macroalgae is increasing the diversity of available prey and facilitates spread of another alien species. Although P. gibbesi is not in direct competition with native sea urchins Paracentrotus lividus and Arbacia lixula, high abundances of this invasive crab are likely to be reducing the energy available to higher consumers in the food web. Key words: invasive alien species, herbivores, trophic interactions, marine protected area, Mediterranean P‐70 DNEVNI PRIRASTI OTOLITA KAO METODA VALIDACIJE TOČNOG ODREĐIVANJA STAROSTI TRLJE OD KAMENA, Mullus surmuletus S. Matić‐Skoko1, J. Ferri2, N. Stagličić1, P. Tutman1, D. Brajčić Jurica3 1 Institut za oceanografiju i ribarstvo, Split, Hrvatska ([email protected]) 2 Sveučilište u Splitu, Odjel za studije mora, Split, Hrvatska 3 Ribarska zadruga Friška riba, Split, Hrvatska Postoji pojačana potreba za relevantnom, vjerodostojnom metodom određivanja starosti kod trlje od kamena, Mullus surmuletus kao ciljane vrste mediteranskog priobalnog ribarstva. Ukupno je analizirano 387 jedinki ukupnih dužina od 6,8 do 32,9 cm. Godišnja periodičnost nastanka prstenova potvrđena je analizom rubnog prirasta. Godišnji rast otolita je verificiran analizom tipa ruba. Udio otolita s tamnim rubom je bio najveći zimi (>80%). Mjesečni srednji rubni prirast je bio najmanji u lipnju. Analiza mjerenih udaljenosti je pokazala da se prvi pravi th
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prsten pojavljuje na udaljenosti od 0,78 mm (± 0,104 SD) od centra i pri starosti ribe od 0,83 godina. Analiza starosti je utvrdila 6 starosnih razreda i točnost je potvrđena brojanjem dnevnih prirasta. Svi su mjereni parametri otolita bili linearni sa starosti. Najpreciznija je procjena za analiziranu populaciju dobivena iz širine otolita, a slijedila je dužina i masa. Rast je procijenjen Von Bertalanffy‐om jednadžbom rasta. Rast je bio najbrži do druge godine života, a onda usporava. Uzimajući u obzir utvrđene biološke odrednice, trenutno gospodarenje je potrebno nadopuniti cjelovitijim, ekološkim pristupom propisima, kako bi se osigurale učinkovite mjere zaštite za ovu ekonomski i ekološki važnu vrstu riba. Ključne riječi: dnevni prirast, starost, otolit, Mullus surmuletus DAILY OTOLITH INCREMENTS AS VALIDATION METHOD FOR AGE DETERMINATION OF THE STRIPED RED MULLET, Mullus surmuletus S. Matić‐Skoko1, J. Ferri2, N. Stagličić1, P. Tutman1, D. Brajčić Jurica3 1 Institute of Oceanography and Fisheries Split, Croatia ([email protected]) 2 University of Split, Center of Marine Studies, Split, Croatia 3 Fishermen Cooperative Fresh Fish, Split, Split, Croatia There is a high need to establish a method for reliable age determination of the striped red mullet, Mullus surmuletus as target fish species of Mediterranean artisanal fisheries. In total, 387 specimens ranging in total length from 6.8 to 32.9 cm were analyzed. The annual periodicity of annulus deposition was supported by the marginal increment analysis. The annual growth of otoliths was verified using edge‐type analysis. The proportion of otoliths with opaque margins was the highest (>80%) in the winter. The monthly mean marginal increment showed a single minimum in June. A distance measurements analysis revealed that first regular ring appears at distance of 0.78 mm (± 0.104 SD) from otolith nucleus and fish age of about 0.83 years. The age analysis revealed 6 age classes. Age determination was validated by daily otoliths increments readings. All measured otolith morphometric parameters were linear with fish age. The most precise age estimations of analyzed population were obtained from the otolith width, followed by the otolith length and mass. The growth was estimated by the fit of the Von Bertalanffy growth function. The growth of M. surmuletus was rapid up to 2 years of age and then slowed. Considering the identified biological implications, existing management should be complemented by a more holistic, ecosystem approach to regulations, in order to assure effective conservation measures for this economically and ecologically important fish species. Key words: daily increments, age, otolith, Mullus surmuletus P‐71 PROCJENA BROJNOSTI ZAJEDNICE DOBRIH DUPINA (Tursiops truncatus) VIŠKOG AKVATORIJA J. Miočić‐Stošić1, G. Pleslić2, N. Rako Gospić2, C.M. Fortuna3, D. Holcer4 1 Plavi svijet Vis, Vis, Hrvatska ([email protected]‐vis.hr) 2 Plavi svijet Institut za istraživanje i zaštitu mora, Veli Lošinj, Hrvatska 3 Institut za zaštitu i istraživanje okoliša, Rim, Italija 4 Hrvatski prirodoslovni muzej, Zagreb, Hrvatska U razdoblju od 2007. do 2014. istraživana je biologija i ekologija dobrih dupina (Tursiops truncatus) u akvatoriju otoka Visa (~5.500 km2). Terensko prikupljanje podataka provedeno je th
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od proljeća do jeseni plovilom duljine 6 m. Opaženo je 327 skupina dobrih dupina, a metodom fotoidentifikacije je utvrđena prisutnost 723 jedinke. Brojnost populacije procijenjena je metodom ulova i ponovnog ulova za zatvorene populacije pri čemu je korišten Chao Mth procjenitelj. U analizu su uvrštene samo jedinke s izraženim prirodnim oznakama kako bi se umanjila vjerojatnost pogrešne identifikacije. Rezultati ukazuju na to da je brojnost populacije u ovom razdoblju bila stabilna budući da usporedbom utvrđenih vrijednosti nije ustanovljena statistički značajna razlika između pojedinih godina (Z‐test; z0,0674 za sve usporedbe). Na temelju podataka iz 2014. godine, procijenjena brojnost populacije iznosi 278 (95% CI=208‐413) jedinki dobrih dupina. Budući da je dio istraživanog područja uvršten u Natura 2000 mrežu s ciljem očuvanja dobrih dupina, potrebno je osigurati nastavak praćenja brojnosti populacije u ovom području. Ključne riječi: dobri dupin, procjena brojnosti, zaštićeno područje ABUNDANCE ESTIMATES OF THE BOTTLENOSE DOLPHIN (Tursiops truncatus) COMMUNITY IN VIS ARCHIPELAGO J. Miočić‐Stošić1, G. Pleslić2, N. Rako Gospić2, C.M. Fortuna3, D. Holcer4 1 Blue World Vis, Vis, Croatia ([email protected]‐vis.hr) 2 Blue World Institute of Marine Research and Conservation, Veli Lošinj, Croatia 3 Institute for Environmental Protection and Research, Rome, Italy 4 Croatian Natural History Museum, Zagreb, Croatia We studied the biology and ecology of the resident population of bottlenose dolphins (Tursiops truncatus) in the area around Vis Island (~5,500 km2) between 2007 and 2014. Data was collected in the spring and summer using a 6 meter rigid inflatable boat. We obtained 327 sightings and photo‐identified 723 individuals. Abundance of the bottlenose dolphin population was estimated using the mark‐recapture model for closed populations and Mth estimator of Chao. To avoid misidentification errors, only well‐marked individuals were included in the analysis. No significant difference in the year‐to‐year abundance was detected (Z‐test; z0.0674 for all comparisons), so the population appeared stable during the study period. Based on data collected in 2014, the population abundance estimate is 278 (95% CI=208‐413) bottlenose dolphins. As a part of the research area was included in the Natura 2000 network with the aim of conserving the local bottlenose dolphin population, it is necessary to continue with monitoring of the population abundance in this area. Key words: bottlenose dolphin, abundance estimate, protected area P‐72 STANJE POPULACIJE INĆUNA (Engraulis encrasicolus L. 1758) U ISTOČNOM DIJELU JADRANSKOG MORA B. Mustać Sveučilište u Zadru, Odjel za ekologiju, agronomiju i akvakulturu, Zadar, Hrvatska ([email protected]) Inćun (brgljun) Engraulis encrasicolus L. 1758., je jedna od najbrojnijih vrsta u ribarskim lovinama RH. Međutim, zadnjih godina se uočava pad ulova ove izrazito značajne vrste za gospodarstvo svih zemalja uz Jadransko more, kao i za ekosustav Jadranskoga mora. Ukupan th
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ulov inćuna u RH je 2011. godine bio 14163 t, dok je 2013. godine iznosio 8904 tone. Svrha je ovog rada utvrditi stanje populacije inćuna u istočnom dijelu Jadrana.Analizirano je ukupno 612 jedinki uzorkovanih od lipnja do prosinca 2014. godine. Svi uzorci su potjecali iz lovina plivarica, ostvarenih u ribolovnim zonama B, C, E i F istočnog, hrvatskog dijela Jadranskoga mora.Totalne dužine (LT) svih jedinki su se kretale u rasponu od 10,0 do 16,6 cm. Srednja vrijednost je iznosila 13,5 ± 0,99 cm, a modalni dužinski razred ‐ 13,0 cm. Odnos spolova je bio u korist mužjaka; iznosio je m/ž=1,63. Analizom dužinsko‐masenog odnosa utvrđen je pozitivan alometrijski rast (b=3,469; r2=0,899). Kubični koeficijent kondicije (K) se kretao u rasponu od 0,52 do 0,69, te je pokazao, ukupno gledajući, povećanje vrijednosti s porastom dužine ribe. Početkom ovog istraživanja, u lipnju, inćun se mrijestio, a faza spolnog mirovanja je započela nakon rujna. Ključne riječi: inćun, Jadransko more, dužinsko‐maseni donos, kondicija ANCHOVY (Engraulis encrasicolus L. 1758) POPULATION STATUS IN THE EASTERN ADRIATIC SEA B. Mustać University of Zadar, Department of ecology, agronomy and aquaculture, Zadar, Croatia ([email protected]) Anchovy Engraulis encrasicolus L. 1758, is one of the most abundant species in Croatian fisheries. However, in the last few years, landings of this ecologically and commercially very important species have decreased from 14163 tons in 2011 to 8904 tons in 2013. The aim of this study was to analyse current state of anchovy population in the Eastern Adriatic Sea. In total, 612 anchovy specimens were sampled from June to December 2014. All fishes were sampled from purse seine landings, i.e. from fisheries zones B, C, E and F of the Eastern Adriatic Sea. Total length (LT) varied from 10.0 to 16.6 cm, the average length was 13.5 ± 0.99 cm, and modal length class ‐ 13.0 cm. Number of male specimens exceeded the number of female specimens (m/f=1.63). Analyses of length‐weight relation indicated positive allometric growth (b=3.469; r2=0.899). Condition coefficient (K) ranged from 0.52 to 0.69. In general, condition values were higher in longer fishes. In the beginning of this study, in June, anchovy was spawning, and inactive phase of maturity cycle started after September. Key words: Anchovy, Adriatic Sea, Length‐weight relation, Condition coefficient P‐73 PRAĆENJE RADIOEKOLOŠKOG STANJA SJEVERNOG JADRANA KORIŠTENJEM DAGNJE Mytilus galloprovincialis KAO INDIKATORA D. Pavičić‐Hamer CIM, IRB, Rovinj, Hrvatska ([email protected]) Izvor radionuklida u Jadranskom moru je atmosfersko zagađivanje, prvenstveno od Černobilske nesreće 1986. godine, a posredni izvori su donosi rijekama. Dagnja Mytilus galloprovincialis se koristi kao indikatorski organizam u monitoringu onečišćenja mora. Program sustavnog ispitivanja radioaktivnosti u morskim indikatorskim organizmima temelji se na određivanju koncentracije aktivnosti umjetnog radionuklida 137Cs, te prirodnih 40K, 232Th (228Ra), 226Ra i 238U i kozmogenog 7Be u tkivu dagnje. Dagnje su uzorkovane u travnju i listopadu od 2008. do 2014. godine na th
12. HBK 209 12 CBC
lokacijama pod izrazitim utjecajem dotoka slatkih voda: Limski kanal, Raški i Bakarski zaljev. U morskoj vodi Limskog kanala koncentracije aktivnosti 137Cs su bile niske (1,83 Bq m‐3). Na svim lokacijama koncentracije aktivnosti 137Cs u dagnji kretale su se ispod granica detekcije (